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    SYSTEM AND METHODS FOR MAKING AND PROCESSING EMULSIONS
    23.
    发明申请
    SYSTEM AND METHODS FOR MAKING AND PROCESSING EMULSIONS 有权
    制备和处理乳液的系统和方法

    公开(公告)号：US20150224457A1

    公开(公告)日：2015-08-13

    申请号：US14696642

    申请日：2015-04-27

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan SCHULTZ ,  John Nobile ,  Brian Reed ,  Prasanna Krishnan Thwar ,  Todd Roswech

    IPC: B01F5/06

    CPC classification number: B01F5/0602 ,  B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F2215/0037 ,  B01L3/502784 ,  B04B5/0414 ,  B04B7/02

    Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

    Abstract translation: 提供了一种自动化的在线模板珠粒制备系统，包括膜基乳液生成子系统，乳液PCR（ePCR）热循环板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式向逆循环子系统提供连续的反相乳液流。 ePCR子系统可以连续热循环通过的反相乳液，并包括静态温度区和可消耗的热循环板。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。

    ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS
    24.
    发明申请
    ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS 有权
    序列合成方法中的替代核酸流

    公开(公告)号：US20130172201A1

    公开(公告)日：2013-07-04

    申请号：US13689252

    申请日：2012-11-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan SCHULTZ ,  John DAVIDSON

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  B01L3/5027 ,  C12Q1/6874 ,  C12Q2537/155 ,  C12Q2563/149 ,  C12Q2565/301 ,  C12Q2565/629

    Abstract: A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.

    Abstract translation: 通过使用按顺序合成技术测序多核苷酸链的方法。 为了解决在逐个合成反应中可能发生的不完全扩展（IE）和/或结转（CF）错误的问题，使用了dNTP的替代流顺序。 与常规流顺序相反，dNTP以不是四种不同dNTP的顺序的连续重复的顺序流动。 这种交替流顺序可以减少由IE和/或CF错误导致的模板多核苷酸链群体中的相位同步损失。

    SEQUENCING DEVICE
    25.
    发明申请
    SEQUENCING DEVICE 有权

    公开(公告)号：US20210130891A1

    公开(公告)日：2021-05-06

    申请号：US17146376

    申请日：2021-01-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan SCHULTZ ,  Todd ROSWECH ,  Jon A. HOSHIZAKI ,  Albert L. CARRILLO ,  James A. BALL

    IPC: C12Q1/6869 ,  G01N27/414 ,  C12Q1/6806 ,  B01F5/06 ,  B01F11/00 ,  B01L3/00 ,  B01F1/00 ,  G01N35/10

    Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

    FLUIDICS SYSTEM FOR SEQUENTIAL DELIVERY OF REAGENTS
    26.
    发明申请
    FLUIDICS SYSTEM FOR SEQUENTIAL DELIVERY OF REAGENTS 审中-公开

    公开(公告)号：US20200086317A1

    公开(公告)日：2020-03-19

    申请号：US16687672

    申请日：2019-11-18

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan SCHULTZ ,  David MARRAN

    IPC: B01L3/00 ,  G01N27/447 ,  C12Q1/6869

    Abstract: The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion. The invention is particularly advantageous in apparatus for performing sensitive multistep reactions, such as pH-based DNA sequencing reactions.

    PHASE-PREOTECTING REAGENT FLOW ORDERING FOR USE IN SEQUENCING-BY-SYNTHESIS
    28.
    发明申请
    PHASE-PREOTECTING REAGENT FLOW ORDERING FOR USE IN SEQUENCING-BY-SYNTHESIS 审中-公开
    相序预处理试剂流顺序用于按顺序合成

    公开(公告)号：US20170044602A1

    公开(公告)日：2017-02-16

    申请号：US15236172

    申请日：2016-08-12

    Applicant: Life Technologies Corporation

    Inventor： Earl HUBBELL ,  Jonathan SCHULTZ

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  C12Q2533/101 ,  C12Q2565/301 ,  C12Q2565/607

    Abstract: A system and machine readable medium for nucleic acid sequencing includes disposing template polynucleotide strands in defined spaces disposed on a sensor array, at least some of the template polynucleotide strands having a sequencing primer and a polymerase operably bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed according to a predetermined ordering; and determining, for each of the series of flows of nucleotide species, how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands, wherein the predetermined ordering (a) is not a series of consecutive repetitions of a 4-flow permutation of four different nucleotide species, (b) is not specifically tailored to a particular combination of a particular template polynucleotide strand to be sequenced and a particular sequencing primer to be used, and (c) comprises a phase-protecting flow ordering.

    Abstract translation: 用于核酸测序的系统和机器可读介质包括在设置在传感器阵列上的限定空间中设置模板多核苷酸链，至少一些模板多核苷酸链具有测序引物和与之可操作地结合的聚合酶; 将模板多核苷酸链暴露于根据预定排序流动的一系列核苷酸类型的流; 并且对于核苷酸种类的每一系列流程，确定针对该特定流出现多少个核苷酸掺入以确定对应于模板多核苷酸链的预测的核苷酸序列，其中预定顺序（a）不是一系列连续的 重复四种不同核苷酸物种的4流置换，（b）不是特定的针对待测序的特定模板多核苷酸链和特定的待测序列的特定组合而定制的，（c） 保护流量顺序。

    CENTRIFUGE AND METHOD FOR LOADING A DEVICE
    29.
    发明申请
    CENTRIFUGE AND METHOD FOR LOADING A DEVICE 审中-公开
    用于加载设备的离散和方法

    公开(公告)号：US20160230214A1

    公开(公告)日：2016-08-11

    申请号：US15133428

    申请日：2016-04-20

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Kristopher BARBEE ,  Ryan JONES ,  Sean MCCUSKER ,  Maximilian CARPINO ,  John LEAMON ,  Jonathan SCHULTZ

    IPC: C12Q1/68 ,  B04B5/04

    Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm.

    Abstract translation: 一种装置包括机器人系统，该机器人系统在三个正交方向上提供可操作以接收移液管尖端的臂并促进流体进入和移出移液管尖端的运动。 此外，该装置可以包括用于接收移液管吸头的托盘，用于接收管的容器，用于形成乳液的装置，用于形成包括多核苷酸的拷贝的颗粒的装置，用于富集颗粒的装置和用于加载颗粒的装置 颗粒到传感器阵列上。 该装置还可以包括容纳试剂溶液容器的容器。 可选地，除了移液管接收臂之外，机器人还可以包括夹持臂。

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