公开(公告)号：US20170313965A1

公开(公告)日：2017-11-02

申请号：US15653967

申请日：2017-07-19

Applicant: OLYMPUS CORPORATION

Inventor： Yasunobu IGA ,  Yohei TANIKAWA ,  Shinichi TAKIMOTO ,  Yoshinobu AKAHORI

IPC: C12M1/34 ,  G01N21/88 ,  G01N21/01

CPC classification number: C12M1/3446 ,  C12M41/46 ,  C12M41/48 ,  G01N21/8851 ,  G01N2021/0143

Abstract: A cell analysis apparatus including: a cell image acquisition unit that acquires an image of cells in a culture vessel in which cells are cultured; a smoothing processing unit that smooths luminance values in the image acquired by the cell image acquisition unit; a minimum-value detecting unit that detects minimum values of the luminance values smoothed by the smoothing processing unit; a smallest-minimum-value extracting unit that extracts smallest minimum values which are the smallest in regions according to the sizes of the cells, from the minimum values detected by the minimum-value detecting unit; a counting unit that counts the number of smallest minimum values extracted by the smallest-minimum-value extracting unit; and a cell-count calculating unit that calculates the number of cells in the culture vessel on the basis of the number of smallest minimum values counted by the counting unit.

公开(公告)号：US20170261732A1

公开(公告)日：2017-09-14

申请号：US15607666

申请日：2017-05-29

Applicant: OLYMPUS CORPORATION

Inventor： Shintaro TAKAHASHI ,  Tadashi HIRATA ,  Yasunobu IGA ,  Shinichi TAKIMOTO ,  Takashi MIYOSHI

IPC: G02B21/06 ,  G02B21/24 ,  C12M1/34 ,  G02B21/36

CPC classification number: G02B21/06 ,  C12M1/34 ,  G02B21/084 ,  G02B21/14 ,  G02B21/24 ,  G02B21/361

Abstract: Provided is an observation apparatus and an observation method with which it is possible to observe imaging subjects, such as cells or the like, without labeling the imaging subjects and without causing an increase in the apparatus size. Provided is an observation apparatus including: a light-source unit that emits illumination light upward from below a sample; and an image-capturing optical system that has an objective lens that collects transmitted light, which is the illumination light emitted from the light-source unit that has passed through the sample by being reflected above the sample and that captures, below the sample, the transmitted light collected by the objective lens.

公开(公告)号：US20170046362A1

公开(公告)日：2017-02-16

申请号：US15246411

申请日：2016-08-24

Applicant: OLYMPUS CORPORATION

Inventor： Yasunobu IGA ,  Yohei TANIKAWA ,  Shinichi TAKIMOTO

IPC: G06F17/30

CPC classification number: G06F16/5866 ,  G06F16/20 ,  G06F16/51 ,  G16B99/00

Abstract: A cell observation information processing system includes: a tag acquiring section that acquires tags, as search tags for searching for observation information acquired in response to an acquisition order at one time point; a storing section that stores into an archive respective sets of the observation information acquired at respective time points upon assigning to them the search tags; a retrieving section that retrieves a list of tags as possible search tags from the archive; and an output section that outputs the list of tags to a display. For retrieving the list of tags, the retrieving section designates a narrowing-down criterion and narrows down tags to the possible search tags using the narrowing-down criterion. The tag acquiring section acquires a tag selected by the user from the list of tags as displayed. This configuration can save operation effort and time of the user taken to acquire tags.

Abstract translation: 小区观测信息处理系统包括：标签获取部，其获取标签，作为用于搜索在一个时间点响应于获取次序获取的观察信息的搜索标签; 存储部，其在向其分配了所述搜索标签的同时，将在各个时间点获取的观察信息的各组中存储; 检索部分，其从归档中检索可能的搜索标签的标签列表; 以及将标签列表输出到显示器的输出部。 为了检索标签列表，检索部分指定缩小标准，并使用缩小标准将标签缩小到可能的搜索标签。 标签获取部分从显示的标签列表中获取用户选择的标签。 该配置可以节省用户获取标签的操作时间和时间。

公开(公告)号：US20160341657A1

公开(公告)日：2016-11-24

申请号：US15227086

申请日：2016-08-03

Applicant: OLYMPUS CORPORATION

Inventor： Akira SATO ,  Shinichi TAKIMOTO

IPC: G01N21/27 ,  G01J3/28

CPC classification number: G01N21/27 ,  G01J3/00 ,  G01J3/28 ,  G01J3/44 ,  G01J2003/283 ,  G01N21/65 ,  G01N2201/12

Abstract: A spectral measurement method of the present invention includes: a measuring step of measuring optical spectra of some sections specified among a plurality of sections on a specimen; a scalar-value calculating step of calculating, for individual measured sections, scalar values that represent information contained in the obtained optical spectra; an interpolating step of interpolating scalar values of unmeasured sections by using the calculated scalar values and by using two types of interpolation methods; an identifying step of identifying sections in which absolute values of differences between the two scalar values interpolated by using the two types of interpolation methods are equal to or greater than a predetermined threshold; and a repeating step of re-executing steps from the measuring step to the identifying step after specifying the identified sections.

Abstract translation: 本发明的光谱测量方法包括：测量步骤，测量在样本上的多个部分之间指定的一些部分的光谱; 标量值计算步骤，对于各个测量部分，计算表示所获得的光谱中包含的信息的标量值; 通过使用所计算的标量值和通过使用两种类型的插值方法来内插未测量部分的标量值的内插步骤; 识别步骤，用于识别通过使用两种类型的插值方法内插的两个标量值之间的差的绝对值等于或大于预定阈值的部分; 以及在指定所识别的部分之后重新执行从测量步骤到识别步骤的步骤的重复步骤。

公开(公告)号：US20160203165A1

公开(公告)日：2016-07-14

申请号：US15079237

申请日：2016-03-24

Applicant: OLYMPUS CORPORATION

Inventor： Yohei TANIKAWA ,  Yasunobu IGA ,  Shinichi TAKIMOTO

IPC: G06F17/30 ,  G06T7/00

CPC classification number: G06F16/5866 ,  G06F16/51 ,  G06F16/9535 ,  G06T7/0012 ,  G06T2207/30024 ,  G16B50/00

Abstract: A cell observation information processing system includes: a storing section that assigns, to each of observation information sets acquired in response to an acquisition order at each time point to carry an observation result containing a cell image and activity data, search tags including an image tag as a main tag and subordinate tags subordinately associated with the image tag, and stores the search-tagged observation information set into an archive; an image selector; a search criterion generator that searches through the archive using the selected image tag, retrieves a matched search-tagged observation information set, and automatically generates a search criterion based on subordinate tags assigned to the search-tagged observation information set having been retrieved; and a retrieving section that searches through the archive using the search criterion and retrieves data containing activity data in observation information sets to which search tags matched with the search criterion have been assigned.

Abstract translation: 小区观测信息处理系统包括：存储部分，其针对每个时间点响应于获取顺序而获取的每个观察信息集，分配包含小区图像和活动数据的观察结果，包括图像标签的搜索标签 作为与图像标签下属关联的主标签和下级标签，并将搜索标记的观察信息集合存储到档案中; 图像选择器 搜索标准生成器，使用所选择的图像标签搜索归档，检索匹配的搜索标记的观察信息集，并且基于分配给已经检索到的检索标签的观察信息集的下级标签自动生成搜索标准; 以及检索部分，其使用搜索标准搜索归档，并且检索包含与分配了搜索标准匹配的搜索标签的观察信息集中的活动数据的数据。

公开(公告)号：US20150008340A1

公开(公告)日：2015-01-08

申请号：US14495241

申请日：2014-09-24

Applicant: OLYMPUS CORPORATION

Inventor： Katsumasa FUJITA ,  Takeharu NAGAI ,  Kenta SAITO ,  Masahito YAMANAKA ,  Shinichi TAKIMOTO

IPC: G01N21/64

CPC classification number: G02B21/0076 ,  A61B6/037 ,  A61B6/0478 ,  A61B6/501 ,  G01N21/6458 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2201/068 ,  G01N2201/0697 ,  G01T1/2985

Abstract: A fluorescence observation method of the present invention for detecting plural types of fluorescence emitted from two or more kinds of fluorescent molecules includes: subjecting each of the two or more kinds of fluorescent molecules to multi-photon excitation by exciting light having an exciting wavelength equal to or shorter than 700 nm in a visible region, to generate fluorescence upon making use of an absorption wavelength band in a deep ultraviolet region of each of the two or more kinds of fluorescent molecules; and simultaneously detecting plural types of fluorescence generated on a shorter-wavelength side or on both of the shorter-wavelength side and a longer-wavelength side of the exciting wavelength of the exciting light.

Abstract translation: 本发明的用于检测从两种以上荧光分子发出的多种荧光的荧光观察方法包括：通过激发具有激发波长的光，使两种或更多种荧光分子中的每一种进行多光子激发 或者在可见光区域中短于700nm，在使用两种或更多种荧光分子的深紫外区域中的吸收波长带产生荧光; 并且同时检测在激发光的激发波长的短波长侧或短波长侧和长波长侧的两端产生的多种荧光。

公开(公告)号：US20140018685A1

公开(公告)日：2014-01-16

申请号：US14030464

申请日：2013-09-18

Applicant: OLYMPUS CORPORATION

Inventor： Kenji TAIRA ,  Hiroyoshi YAJIMA ,  Shinichi TAKIMOTO

IPC: G01J1/04 ,  A61B1/04 ,  A61B5/00

CPC classification number: G01J1/0407 ,  A61B1/04 ,  A61B1/043 ,  A61B1/07 ,  A61B5/0066 ,  A61B5/0068 ,  A61B5/0071 ,  A61B5/0073 ,  A61B5/0075 ,  A61B5/0077 ,  A61B5/0084 ,  A61B5/489 ,  A61B5/6852 ,  G01N21/4795 ,  G01N21/6458 ,  G01N21/65 ,  G01N2021/6484 ,  G01N2021/653 ,  G01N2021/655

Abstract: An optical inspection device 1, comprising a light generation means 2, a light irradiation means 3 irradiating an object to be inspected 4 with light generated from the light generation means 2 and a photodetection means 6 photoelectrically converting signal light obtained from the object to be inspected 4 through irradiation of light by the light irradiation means 3, and inspecting the object to be inspected 4 based on output from the photodetection means 6, wherein a light amplification means 5 amplifying signal light obtained from the object to be inspected 4 is provided. There is thus provided an optical inspection device capable of photoelectically converting signal light from the object to be inspected with high sensitivity and promptly with its inexpensive configuration without increasing the intensity of light with which the object to be inspected is irradiated and without using an expensive low-noise and high-sensitivity photodetector.

Abstract translation: 一种光学检查装置1，其包括光发生装置2，用从发光装置2产生的光照射被检查物体4的光照射装置3以及对从被检查物体获得的信号光进行光电转换的光检测装置6 通过光照射装置3照射光，并且根据来自光检测装置6的输出检查待检查对象4，其中提供放大从被检查对象4获得的信号光的光放大装置5。 因此，提供了一种光学检查装置，其能够以高灵敏度和及时地以其便宜的构造对来自被检查物体的信号光进行光电转换，而不增加被照射物体照射的光的强度，并且不使用昂贵的低 噪声和高灵敏度光电探测器。