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公开(公告)号:US20120115738A1
公开(公告)日:2012-05-10
申请号:US12249872
申请日:2008-10-10
申请人: Peng Zhou , Lincoln C. Young , Todd Roswech , Gwendolyn Spizz , Zongyuan Chen , Benjamin W. Thomas , Travis Lee
发明人: Peng Zhou , Lincoln C. Young , Todd Roswech , Gwendolyn Spizz , Zongyuan Chen , Benjamin W. Thomas , Travis Lee
CPC分类号: B01F13/0059 , B01F5/0683 , B01F5/0688 , B01F11/0074 , B01L3/50273 , B01L7/52 , B01L2200/027 , B01L2200/0621 , B01L2200/10 , B01L2300/0816 , B01L2300/1816 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2300/185 , B01L2400/0487 , B01L2400/0638 , B01L2400/084
摘要: A microfluidic device for analyzing a sample of interest is provided. The microfluidic device can comprise a microfluidic device body, wherein the microfluidic device body comprises a sample preparation area, a nucleic acid amplification area, a nucleic acid analysis area, and a network of fluid channels. Each of the sample preparation area, the nucleic acid amplification area and the nucleic acid analysis area are fluidly interconnected to at least one of the other two areas by at least one of the fluid channels. Using the microfluidic device, sample preparation can be combined with amplification of a biologically active molecule, and a suitable biological sample can be provided for analysis and/or detection of a molecule of interest. The small-scale apparatus and methods provided are easier, faster, less expensive, and equally efficacious compared to larger scale equipment for the preparation and analysis of a biological sample.
摘要翻译: 提供了用于分析感兴趣样品的微流体装置。 微流体装置可以包括微流体装置主体,其中微流体装置主体包括样品制备区域,核酸扩增区域,核酸分析区域和流体通道网络。 样品制备区域,核酸扩增区域和核酸分析区域中的每一个通过至少一个流体通道流体地互连到另外两个区域中的至少一个区域。 使用微流体装置,样品制备可以与生物活性分子的扩增相结合,并且可以提供合适的生物样品用于分析和/或检测目的分子。 与用于制备和分析生物样品的较大规模设备相比,提供的小型装置和方法更容易,更快,更便宜,并且同样有效。
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公开(公告)号:US20110214745A1
公开(公告)日:2011-09-08
申请号:US13111137
申请日:2011-05-19
申请人: Peng Zhou , Lincoln C. Young
发明人: Peng Zhou , Lincoln C. Young
IPC分类号: F17D3/00
CPC分类号: G01N1/28 , B01L3/5025 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/00 , B01L2200/026 , B01L2200/027 , B01L2200/028 , B01L2200/04 , B01L2200/0621 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/0887 , B01L2300/1827 , B01L2400/0481 , B01L2400/0622 , B01L2400/0633 , B01L2400/0655 , B01L2400/0666 , B01L2400/084 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0059 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T436/117497 , Y10T436/2575
摘要: The systems and methods described herein include a microfluidic chip having a plurality of microfeatures interconnected to provide a configurable fluid transport system for processing at least one reagent. Inserts are provided to removably interfit into one or more of the microfeatures of the chip, wherein the inserts include sites for interactions with the reagent. As will be seen from the following description, the microfluidic chip and the inserts provide an efficient and accurate approach for conducting parallel assays.
摘要翻译: 本文所述的系统和方法包括具有互连以提供用于处理至少一种试剂的可配置流体输送系统的多个微特征的微流体芯片。 提供插入件可移除地与芯片的一个或多个微特征相配合,其中插入物包括与试剂相互作用的位点。 从以下描述可以看出,微流体芯片和插入物提供用于进行平行测定的有效和准确的方法。
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公开(公告)号:US20090294055A1
公开(公告)日:2009-12-03
申请号:US12539797
申请日:2009-08-12
申请人: Peng Zhou , Lincoln C. Young
发明人: Peng Zhou , Lincoln C. Young
IPC分类号: B32B37/12
CPC分类号: B32B37/0038 , B01F5/0683 , B01F5/0688 , B01F11/0071 , B01F13/0059 , B01L3/502707 , B01L2200/12 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/0887 , B01L2400/0481 , B01L2400/0655 , B01L2400/084 , B29C65/4895 , B29C66/1122 , B29C66/54 , B29C66/543 , B29C66/71 , B29C66/83411 , B29C66/8362 , B29L2031/756 , F04B43/043 , F04B43/06 , F16K99/0001 , F16K99/0015 , F16K99/0021 , F16K99/0034 , F16K99/0055 , F16K99/0057 , F16K2099/0074 , F16K2099/0078 , F16K2099/0084 , F16K2099/0094 , Y10T156/1002 , B29K2069/00 , B29K2033/08 , B29K2025/06
摘要: A method for making a polymeric microfluidic structure in which two or more components (layers) of the microfluidic structure are fixedly bonded or laminated with a weak solvent bonding agent, particularly acetonitrile or a mixture of acetonitrile and alcohol. In an aspect, acetonitrile can be used as a weak solvent bonding agent to enclose a microstructure fabricated in or on a non-elastomeric polymer such as polystyrene, polycarbonate, acrylic or other linear polymer to form a three-dimensional microfluidic network. The method involves the steps of wetting at least one of the opposing surfaces of the polymeric substrate components with the weak solvent bonding agent in a given, lower temperature range, adjacently contacting the opposing surfaces, and thermally activating the bonding agent at a higher temperature than the lower temperature range for a given period of time. The contacted polymeric substrates may also be aligned prior to thermal activation and compressed during thermal activation. A laminated, polymeric microfluidic structure is also disclosed.
摘要翻译: 一种制备聚合微流体结构的方法,其中微流体结构的两个或多个组分(层)用弱溶剂粘合剂,特别是乙腈或乙腈和醇的混合物固定粘合或层压。 在一个方面,乙腈可以用作弱溶剂粘合剂以包围在非弹性聚合物如聚苯乙烯,聚碳酸酯,丙烯酸或其它线性聚合物中或其上制造的微结构,以形成三维微流体网络。 该方法包括以下步骤:在给定的较低温度范围内用弱溶剂粘合剂润湿聚合物基材组分的至少一个相对表面,相邻接触相对表面,并在比 在一定时间内较低的温度范围。 接触的聚合物基材还可以在热活化之前对准,并在热活化期间被压缩。 还公开了层压聚合物微流体结构。
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公开(公告)号:US07608160B2
公开(公告)日:2009-10-27
申请号:US10964216
申请日:2004-10-13
申请人: Peng Zhou , Lincoln C. Young
发明人: Peng Zhou , Lincoln C. Young
IPC分类号: B32B38/04
CPC分类号: B32B37/0038 , B01F5/0683 , B01F5/0688 , B01F11/0071 , B01F13/0059 , B01L3/502707 , B01L2200/12 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/0887 , B01L2400/0481 , B01L2400/0655 , B01L2400/084 , B29C65/4895 , B29C66/1122 , B29C66/54 , B29C66/543 , B29C66/71 , B29C66/83411 , B29C66/8362 , B29L2031/756 , F04B43/043 , F04B43/06 , F16K99/0001 , F16K99/0015 , F16K99/0021 , F16K99/0034 , F16K99/0055 , F16K99/0057 , F16K2099/0074 , F16K2099/0078 , F16K2099/0084 , F16K2099/0094 , Y10T156/1002 , B29K2069/00 , B29K2033/08 , B29K2025/06
摘要: A method for making a polymeric microfluidic structure in which two or more components (layers) of the microfluidic structure are fixedly bonded or laminated with a weak solvent bonding agent, particularly acetonitrile or a mixture of acetonitrile and alcohol. In an aspect, acetonitrile can be used as a weak solvent bonding agent to enclose a microstructure fabricated in or on a non-elastomeric polymer such as polystyrene, polycarbonate, acrylic or other linear polymer to form a three-dimensional microfluidic network. The method involves the steps of wetting at least one of the opposing surfaces of the polymeric substrate components with the weak solvent bonding agent in a given, lower temperature range, adjacently contacting the opposing surfaces, and thermally activating the bonding agent at a higher temperature than the lower temperature range for a given period of time. The contacted polymeric substrates may also be aligned prior to thermal activation and compressed during thermal activation. A laminated, polymeric microfluidic structure is also disclosed.
摘要翻译: 一种制备聚合微流体结构的方法,其中微流体结构的两个或多个组分(层)用弱溶剂粘合剂,特别是乙腈或乙腈和醇的混合物固定粘合或层压。 在一个方面,乙腈可以用作弱溶剂粘合剂以包围在非弹性聚合物如聚苯乙烯,聚碳酸酯,丙烯酸或其它线性聚合物中或其上制造的微结构,以形成三维微流体网络。 该方法包括以下步骤:在给定的较低温度范围内用弱溶剂粘合剂润湿聚合物基材组分的至少一个相对表面,相邻接触相对表面,并在比 给定时间段的较低温度范围。 接触的聚合物基材还可以在热活化之前对准,并在热活化期间被压缩。 还公开了层压聚合物微流体结构。
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公开(公告)号:US08715447B2
公开(公告)日:2014-05-06
申请号:US13185619
申请日:2011-07-19
申请人: Peng Zhou , Lincoln C. Young
发明人: Peng Zhou , Lincoln C. Young
IPC分类号: B32B38/04
CPC分类号: B32B37/0038 , B01F5/0683 , B01F5/0688 , B01F11/0071 , B01F13/0059 , B01L3/502707 , B01L2200/12 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/0887 , B01L2400/0481 , B01L2400/0655 , B01L2400/084 , B29C65/4895 , B29C66/1122 , B29C66/54 , B29C66/543 , B29C66/71 , B29C66/83411 , B29C66/8362 , B29L2031/756 , F04B43/043 , F04B43/06 , F16K99/0001 , F16K99/0015 , F16K99/0021 , F16K99/0034 , F16K99/0055 , F16K99/0057 , F16K2099/0074 , F16K2099/0078 , F16K2099/0084 , F16K2099/0094 , Y10T156/1002 , B29K2069/00 , B29K2033/08 , B29K2025/06
摘要: A method for making a polymeric microfluidic structure in which two or more components (layers) of the microfluidic structure are fixedly bonded or laminated with a weak solvent bonding agent, particularly acetonitrile or a mixture of acetonitrile and alcohol. In an aspect, acetonitrile can be used as a weak solvent bonding agent to enclose a microstructure fabricated in or on a non-elastomeric polymer such as polystyrene, polycarbonate, acrylic or other linear polymer to form a three-dimensional microfluidic network. The method involves the steps of wetting at least one of the opposing surfaces of the polymeric substrate components with the weak solvent bonding agent in a given, lower temperature range, adjacently contacting the opposing surfaces, and thermally activating the bonding agent at a higher temperature than the lower temperature range for a given period of time. The contacted polymeric substrates may also be aligned prior to thermal activation and compressed during thermal activation. A laminated, polymeric microfluidic structure is also disclosed.
摘要翻译: 一种制备聚合微流体结构的方法,其中微流体结构的两个或多个组分(层)用弱溶剂粘合剂,特别是乙腈或乙腈和醇的混合物固定粘合或层压。 在一个方面,乙腈可以用作弱溶剂粘合剂以包围在非弹性聚合物如聚苯乙烯,聚碳酸酯,丙烯酸或其它线性聚合物中或其上制造的微结构,以形成三维微流体网络。 该方法包括以下步骤:在给定的较低温度范围内用弱溶剂粘合剂润湿聚合物基材组分的至少一个相对表面,相邻接触相对表面,并在比 在一定时间内较低的温度范围。 接触的聚合物基材还可以在热活化之前对准,并在热活化期间被压缩。 还公开了层压聚合物微流体结构。
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26.
公开(公告)号:US08715446B2
公开(公告)日:2014-05-06
申请号:US13010014
申请日:2011-01-20
申请人: Todd Roswech , Peng Zhou , Lincoln C. Young
发明人: Todd Roswech , Peng Zhou , Lincoln C. Young
IPC分类号: B32B38/04
CPC分类号: F16K99/0021 , B01F5/0688 , B01L3/502707 , B01L2200/12 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/0887 , B01L2400/0481 , B01L2400/0655 , B01L2400/084 , B29C65/4895 , B29C66/1122 , B29C66/54 , B29C66/5412 , B29C66/71 , B29C66/73161 , B29C66/953 , B29L2031/756 , F04B43/043 , F04B43/06 , F16K99/0001 , F16K99/0015 , F16K99/0034 , F16K99/0055 , F16K99/0057 , F16K2099/0074 , F16K2099/0078 , F16K2099/0084 , F16K2099/0094 , Y10T156/1002 , B29K2023/38
摘要: A latent solvent-based microfluidic apparatus and method involves laminate bonding of two non-elastomeric, cyclic olefin copolymer (COC) components having opposing surfaces to be bonded, wherein in a contacted state there is at least one interstitial space between the contacted surfaces, applying an organic latent solvent to at least one of the opposing surfaces, wherein the latent solvent is in an inactive state, contacting the two opposing surfaces together, actively removing the latent solvent from the at least one interstitial space; and adjusting a latent solvency parameter to activate the latent solvent, wherein the opposing contacted surfaces become bonded.
摘要翻译: 潜在的溶剂型微流体装置和方法涉及两种具有相互接合表面的非弹性环状烯烃共聚物(COC)组分的层压结合,其中在接触状态下,在接触表面之间存在至少一个间隙空间, 一种有机潜溶剂,其中所述潜在溶剂处于非活性状态,使所述两个相对的表面接触在一起,从所述至少一个间隙空间主动去除所述潜在溶剂; 以及调整潜在溶解度参数以激活所述潜在溶剂,其中所述相对的接触表面变粘合。
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公开(公告)号:US08609039B2
公开(公告)日:2013-12-17
申请号:US13331211
申请日:2011-12-20
申请人: Peng Zhou , Lincoln C. Young
发明人: Peng Zhou , Lincoln C. Young
CPC分类号: F04B43/043 , B01L3/5025 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/00 , B01L2200/027 , B01L2200/028 , B01L2200/04 , B01L2200/0621 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/0887 , B01L2300/1827 , B01L2400/0481 , B01L2400/0622 , B01L2400/0633 , B01L2400/0655 , B01L2400/0666 , B01L2400/084 , F16K99/0001 , F16K99/0015 , F16K99/0059 , F16K2099/0084 , F16K2099/0094 , G01N21/5907
摘要: The systems and methods disclosed herein include a microfluidic system, comprising a pneumatic manifold having a plurality of apertures, and a chip manifold having channels disposed therein for routing pneumatic signals from respective ones of the apertures to a plurality of valves in a microfluidic chip, wherein the channels route the pneumatic signals in accordance with a configuration of the plurality of valves in the microfluidic chip.
摘要翻译: 本文公开的系统和方法包括微流体系统,其包括具有多个孔的气动歧管和具有设置在其中的通道的芯片歧管,用于将来自相应孔的气动信号路由到微流体芯片中的多个阀,其中 通道根据微流体芯片中的多个阀的结构来传送气动信号。
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公开(公告)号:US08372355B2
公开(公告)日:2013-02-12
申请号:US13111137
申请日:2011-05-19
申请人: Peng Zhou , Lincoln C. Young
发明人: Peng Zhou , Lincoln C. Young
IPC分类号: B01L3/00
CPC分类号: G01N1/28 , B01L3/5025 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/00 , B01L2200/026 , B01L2200/027 , B01L2200/028 , B01L2200/04 , B01L2200/0621 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/0887 , B01L2300/1827 , B01L2400/0481 , B01L2400/0622 , B01L2400/0633 , B01L2400/0655 , B01L2400/0666 , B01L2400/084 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0059 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T436/117497 , Y10T436/2575
摘要: The systems and methods described herein include a microfluidic chip having a plurality of microfeatures interconnected to provide a configurable fluid transport system for processing at least one reagent. Inserts are provided to removably interfit into one or more of the microfeatures of the chip, wherein the inserts include sites for interactions with the reagent. As will be seen from the following description, the microfluidic chip and the inserts provide an efficient and accurate approach for conducting parallel assays.
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公开(公告)号:US08323586B2
公开(公告)日:2012-12-04
申请号:US13189787
申请日:2011-07-25
申请人: Peng Zhou , Lincoln C. Young
发明人: Peng Zhou , Lincoln C. Young
IPC分类号: B01L3/00
CPC分类号: G01N1/28 , B01L3/5025 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/00 , B01L2200/026 , B01L2200/027 , B01L2200/028 , B01L2200/04 , B01L2200/0621 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/0887 , B01L2300/1827 , B01L2400/0481 , B01L2400/0622 , B01L2400/0633 , B01L2400/0655 , B01L2400/0666 , B01L2400/084 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0059 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T436/117497 , Y10T436/2575
摘要: The systems and methods described herein include a microfluidic chip having a plurality of microfeatures interconnected to provide a configurable fluid transport system for processing at least one reagent. Inserts are provided to removably interfit into one or more of the microfeatures of the chip, wherein the inserts include sites for interactions with the reagent. As will be seen from the following description, the microfluidic chip and the inserts provide an efficient and accurate approach for conducting parallel assays.
摘要翻译: 本文所述的系统和方法包括具有互连以提供用于处理至少一种试剂的可配置流体输送系统的多个微特征的微流体芯片。 提供插入件可移除地与芯片的一个或多个微特征相配合,其中插入物包括与试剂相互作用的位点。 从以下描述可以看出,微流体芯片和插入物提供用于进行平行测定的有效和准确的方法。
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公开(公告)号:US08293053B2
公开(公告)日:2012-10-23
申请号:US12248521
申请日:2008-10-09
申请人: Lincoln C. Young , Peng Zhou
发明人: Lincoln C. Young , Peng Zhou
IPC分类号: B29C65/54
CPC分类号: F16K7/12 , B01F5/0683 , B01F5/0688 , B01F11/0071 , B01F13/0059 , B01F2215/0034 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2200/12 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/0887 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , B01L2400/084 , B29C65/4895 , B29C66/004 , B29C66/54 , B29C66/5412 , B29C66/71 , B29K2025/00 , B29K2025/04 , B29K2033/08 , B29K2069/00 , B29K2101/12 , B29L2031/7496 , B29L2031/7506 , B29L2031/756 , B81B3/0021 , B81B2201/036 , B81B2201/054 , B81B2203/0127 , B81C1/00103 , B81C1/00158 , F04B43/028 , F04B43/043 , F04B43/06 , F15C1/08 , F16K99/0001 , F16K99/0015 , F16K99/0021 , F16K99/0034 , F16K99/0055 , F16K99/0057 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0094 , Y10T29/49405 , Y10T137/212 , Y10T137/2202 , Y10T137/2218 , Y10T137/8593 , Y10T137/85978 , Y10T156/1002 , Y10T156/1043 , B29K2033/12 , B29K2025/06
摘要: Plastic microfluidic structures having a substantially rigid diaphragm that actuates between a relaxed state wherein the diaphragm sits against the surface of a substrate and an actuated state wherein the diaphragm is moved away from the substrate. As will be seen from the following description, the microfluidic structures formed with this diaphragm provide easy to manufacture and robust systems, as well readily made components such as valves and pumps.
摘要翻译: 塑料微流体结构具有基本上刚性的膜片,其在松弛状态之间起作用,其中隔膜抵靠衬底的表面,以及致动状态,其中隔膜移动离开衬底。 从以下描述可以看出,用这种隔膜形成的微流体结构提供易于制造和坚固的系统,以及易于制造的部件,例如阀和泵。
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