Method for making a semiconductor device that has a dual damascene interconnect
    21.
    发明授权
    Method for making a semiconductor device that has a dual damascene interconnect 失效
    制造具有双镶嵌互连的半导体器件的方法

    公开(公告)号:US06448185B1

    公开(公告)日:2002-09-10

    申请号:US09872109

    申请日:2001-06-01

    IPC分类号: H01L21302

    摘要: An improved method of forming a semiconductor device is described. In that method, a dielectric layer that comprises a carbon doped oxide is formed on a substrate. After a first etched region is formed in the dielectric layer, that region is filled with a sacrificial light absorbing material. A layer of photoresist is then deposited and patterned, followed by forming a second etched region by removing part of the sacrificial light absorbing material and a second part of the dielectric layer. Remaining portions of the photoresist are then removed by exposing the resulting device to a plasma generated from a forming gas. The device is then exposed to a solution for removing the remaining portions of the sacrificial light absorbing material.

    摘要翻译: 描述了一种形成半导体器件的改进方法。 在该方法中,在基板上形成包含碳掺杂氧化物的电介质层。 在电介质层中形成第一蚀刻区域之后,该区域填充牺牲光吸收材料。 然后沉积和图案化一层光致抗蚀剂,然后通过去除部分牺牲光吸收材料和介电层的第二部分形成第二蚀刻区域。 然后通过将所得到的装置暴露于由成形气体产生的等离子体来除去光致抗蚀剂的剩余部分。 然后将该装置暴露于溶液以除去牺牲光吸收材料的剩余部分。

    Dull1 coding for a novel starch synthase and uses thereof
    22.
    发明授权
    Dull1 coding for a novel starch synthase and uses thereof 失效
    Dull1编码新型淀粉合成酶及其用途

    公开(公告)号:US5981728A

    公开(公告)日:1999-11-09

    申请号:US968542

    申请日:1997-11-12

    摘要: The maize gene dull1 (du1) of the present invention is a determinant of the structure of endosperm starch. Mutations of du1 affect the activity of at least two enzymes involved in starch biosynthesis, namely the starch synthase, SSII, and the starch branching enzyme, SBEIIa. Du1 codes for a predicted 1674 residue protein, and is expressed with a unique temporal pattern in endosperm but is undetectable in leaf or root. The size of the Du1 product and its expression pattern match precisely the known characteristics of maize SSII. The Du1 product contains two different repeated regions in its unique amino terminus, one of which is identical to a conserved segment of the starch debranching enzymes. The cDNA provided for in the present invention encodes SSII, and mutations within this gene affect multiple aspects of starch biogenesis by disrupting an enzyme complex containing starch synthase(s), starch branching enzyme(s), and possibly starch debranching enzyme(s).

    摘要翻译: 本发明的玉米基因dull1(du1)是胚乳淀粉结构的决定因素。 du1的突变影响淀粉生物合成中至少两种酶的活性,即淀粉合成酶,SSII和淀粉分支酶SBEIIa。 Du1编码预测的1674残基蛋白,并且在胚乳中以独特的时间模式表达,但在叶或根中是不可检测的。 Du1产品的大小及其表达模式与玉米SSII的已知特征精确匹配。 Du1产品在其独特的氨基末端含有两个不同的重复区域,其中之一与淀粉脱支酶的保守区段相同。 本发明中提供的cDNA编码SSII,并且该基因内的突变通过破坏含有淀粉合成酶,淀粉分支酶和可能的淀粉脱支酶的酶复合物影响淀粉生物发生的多个方面。

    Identification and characterization of a novel alpha-amylase from maize endosperm
    24.
    发明授权
    Identification and characterization of a novel alpha-amylase from maize endosperm 失效
    来自玉米胚乳的新型α-淀粉酶的鉴定和表征

    公开(公告)号:US07270988B2

    公开(公告)日:2007-09-18

    申请号:US10952551

    申请日:2004-09-27

    摘要: SHE, a Starch Hydrolytic Enzyme active in maize endosperm (Zea mays), and the cDNA sequence encoding SHE are disclosed. The specificity of native, purified SHE is similar, in general terms, to previously known alpha-amylases. However, the activity of SHE toward amylopectin results in hydrolysis products that are distinctly different from those of other alpha-amylases. SHE, and its homologous equivalents in other plants such as rice, Arabidopsis, apple and potato, can be used in starch processing for generating different, e.g., larger sized, alpha-limit dextrins for industrial use, as compared to those generated by previously known alpha-amylases or other starch hydrolytic enzymes. In addition, modification of the expression of this enzyme in transgenic maize plants or in other transgenic organisms (including bacteria, yeast, and other plant species) can be useful for the generation of novel starch forms or altered starch metabolism.

    摘要翻译: SHE,在玉米胚乳(Zea mays)中活性的淀粉水解酶,以及编码SHE的cDNA序列。 天然纯化的SHE的特异性通常与以前已知的α-淀粉酶相似。 然而,SHE对支链淀粉的活性导致与其它α-淀粉酶明显不同的水解产物。 SHE及其在其他植物如水稻,拟南芥,苹果和马铃薯中的同源等同物可用于淀粉加工中,用于产生不同的,例如较大尺寸的工业用α限制糊精,与之前已知的 α-淀粉酶或其他淀粉水解酶。 此外,在转基因玉米植物或其他转基因生物(包括细菌,酵母和其他植物物种)中修饰该酶的表达可用于产生新的淀粉形式或改变的淀粉代谢。

    Isolation of SU1, a starch debranching enzyme, the product of the maize gene sugary1
    25.
    发明授权
    Isolation of SU1, a starch debranching enzyme, the product of the maize gene sugary1 失效
    分离SU1,淀粉脱支酶,玉米基因的产物含糖1

    公开(公告)号:US06995300B2

    公开(公告)日:2006-02-07

    申请号:US10179562

    申请日:2002-06-25

    摘要: SU1, a starch debranching enzyme active in maize endosperm (Zea mays), and the cDNA and genomic sequences encoding SU1 are disclosed. The amino acid sequence is significantly similar to that of bacterial isoamylases, enzymes that hydrolyze α-(1→6) glycosidic bonds. Amino acid sequence similarity establishes SU1 as a member of the α-amylase superfamily of starch hydrolytic enzymes. Also disclosed are antibodies reactive with the SU1 protein, methods of producing antibodies to the SU1 protein, methods of producing fusion proteins including SU1 as well as recombinant SU1 and methods of producing transgenic plants with a modified Su1 gene. The native or expressed SU1 protein can serve as a replacement for the bacterial and fungal enzymes currently used in the starch processing industry.

    摘要翻译: SU1是在玉米胚乳(Zea mays)中活性的淀粉脱支酶,并且公开了编码SU1的cDNA和基因组序列。 氨基酸序列与细菌异戊酰酶(水解α-(1-> 6)糖苷键的酶)显着相似。 氨基酸序列相似性建立了SU1作为淀粉水解酶的α-淀粉酶超家族的成员。 还公开了与SU1蛋白反应的抗体,产生SU1蛋白的抗体的方法,产生包括SU1的融合蛋白的方法以及重组SU1以及用修饰的Su1基因产生转基因植物的方法。 天然或表达的SU1蛋白可以作为淀粉加工业目前使用的细菌和真菌酶的替代物。

    Isolation of SU1, a starch debranching enzyme, the product of the maize gene sugaryl
    26.
    发明授权
    Isolation of SU1, a starch debranching enzyme, the product of the maize gene sugaryl 失效
    分离SU1,淀粉脱支酶,玉米基因的产物含糖1

    公开(公告)号:US06410716B1

    公开(公告)日:2002-06-25

    申请号:US09256741

    申请日:1999-02-24

    IPC分类号: A01H400

    摘要: SU1, a starch debranching enzyme active in maize endosperm (Zea mays), and the cDNA and genomic sequences encoding SU1 are disclosed. The amino acid sequence is significantly similar to that of bacterial isoamylases, enzymes that hydrolyze &agr;-(1→6) glycosidic bonds. Amino acid sequence similarity establishes SU1 as a member of the &agr;-amylase superfamily of starch hydrolytic enzymes. Also disclosed are antibodies reactive with the SU1 protein, methods of producing antibodies to the SU1 protein, methods of producing fusion proteins including SU1 as well as recombinant SU1 and methods of producing transgenic plants with a modified su1 gene. The native or expressed SU1 protein can serve as a replacement for the bacterial and fungal enzymes currently used in the starch processing industry.

    摘要翻译: SU1是在玉米胚乳(Zea mays)中活性的淀粉脱支酶,并且公开了编码SU1的cDNA和基因组序列。 氨基酸序列与细菌异戊酰酶(水解α-(1-> 6)糖苷键的酶)显着相似。 氨基酸序列相似性建立了SU1作为淀粉水解酶的α-淀粉酶超家族的成员。 还公开了与SU1蛋白反应的抗体,产生SU1蛋白的抗体的方法,产生包括SU1的融合蛋白的方法以及重组SU1以及用修饰的su1基因产生转基因植物的方法。 天然或表达的SU1蛋白可以作为淀粉加工业目前使用的细菌和真菌酶的替代物。