公开(公告)号：US09926598B2

公开(公告)日：2018-03-27

申请号：US14575808

申请日：2014-12-18

Applicant: Illumina, Inc.

Inventor： Hongxia Xu ,  Alex Aravanis ,  Shengrong Lin

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2565/543

Abstract: The present disclosure relates to the field of molecular biology and more specifically to methods for capturing, amplifying and sequencing target polynucleotides on a solid surface.

公开(公告)号：US20150197798A1

公开(公告)日：2015-07-16

申请号：US14575808

申请日：2014-12-18

Applicant: ILLUMINA, INC.

Inventor： Hongxia Xu ,  Alex Aravanis ,  Shengrong Lin

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2565/543

Abstract: The present disclosure relates to the field of molecular biology and more specifically to methods for capturing, amplifying and sequencing target polynucleotides on a solid surface.

Abstract translation: 本公开涉及分子生物学领域，更具体地涉及在固体表面上捕获，扩增和测序靶多核苷酸的方法。

公开(公告)号：US20240392284A1

公开(公告)日：2024-11-28

申请号：US18795512

申请日：2024-08-06

Applicant: ILLUMINA, INC.

Inventor： Fiona Kaper ,  Jian-Bing Fan ,  Neeraj Salathia ,  Gordon M. Cann ,  Arash Jamshidi ,  Alex Aravanis

IPC: C12N15/10 ,  C12Q1/6876

Abstract: Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).

公开(公告)号：US20210108262A1

公开(公告)日：2021-04-15

申请号：US17076715

申请日：2020-10-21

Applicant: Illumina, Inc.

Inventor： Sante Gnerre ,  Byoungsok Jung ,  Emrah Kostem ,  Alex Aravanis ,  Alex So ,  Xuyu Cai ,  Zhihong Zhang ,  Frank J. Steemers

IPC: C12Q1/6869 ,  G16B30/00 ,  C12Q1/6806 ,  C12N15/10 ,  C12Q1/6855

Abstract: The disclosed embodiments concern methods, apparatus, systems and computer program products for determining sequences of interest using unique molecular index (UMI) sequences that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies and long sequence length. In some implementations, the UMIs include both physical UMIs and virtual UMIs. In some implementations, the unique molecular index sequences include non-random sequences. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.

公开(公告)号：US10975371B2

公开(公告)日：2021-04-13

申请号：US14855207

申请日：2015-09-15

Applicant: ILLUMINA, INC.

Inventor： Neeraj Salathia ,  Jian-Bing Fan ,  Fiona Kaper ,  Gordon M. Cann ,  Arash Jamshidi ,  Alex Aravanis

IPC: C12N15/10 ,  C12Q1/6869 ,  C12Q1/6853 ,  C12Q1/6806

Abstract: Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).

公开(公告)号：US20210010073A1

公开(公告)日：2021-01-14

申请号：US16609869

申请日：2019-03-20

Applicant: Illumina, Inc.

Inventor： Hongxia Xu ,  Dan Cao ,  Alex Aravanis

IPC: C12Q1/6869 ,  C12Q1/6806 ,  C12N9/12 ,  C40B40/08

Abstract: Some embodiments of the methods and compositions provided herein relate to the preparation and use of nucleic acid libraries derived from RNA and DNA. In some embodiments, a nucleic acid library can be prepared by tagging polynucleotides derived from RNA. Some embodiments include the analysis of sequence data from such libraries.

公开(公告)号：US10457969B2

公开(公告)日：2019-10-29

申请号：US14804068

申请日：2015-07-20

Applicant: Illumina, Inc.

Inventor： Gordon M. Cann ,  Jeffrey G. Mandell ,  Alex Aravanis ,  Steven Norberg ,  Dmitry K. Pokholok ,  Frank J. Steemers ,  Farnaz Absalan ,  Leila Bazargan

IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/6816 ,  C12Q1/683 ,  C12Q1/6869

Abstract: A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

公开(公告)号：US12179190B2

公开(公告)日：2024-12-31

申请号：US18087499

申请日：2022-12-22

Applicant: ILLUMINA, INC.

Inventor： Alex Aravanis ,  Boyan Boyanov ,  M. Shane Bowen ,  Dale Buermann ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Poorya Sabounchi ,  Hai Quang Tran

IPC: B01L3/00 ,  B01L7/00 ,  F16K99/00

Abstract: Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.

公开(公告)号：US20210198659A1

公开(公告)日：2021-07-01

申请号：US17197836

申请日：2021-03-10

Applicant: Illumina, Inc.

Inventor： Neeraj Salathia ,  Jian-Bing Fan ,  Fiona Kaper ,  Gordon M. Cann ,  Arash Jamshidi ,  Alex Aravanis

IPC: C12N15/10 ,  C12Q1/6869 ,  C12Q1/6853 ,  C12Q1/6806

Abstract: Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).

公开(公告)号：US20200206737A1

公开(公告)日：2020-07-02

申请号：US16818953

申请日：2020-03-13

Applicant: Illumina, Inc.

Inventor： Sebastian Bohm ,  Alex Aravanis ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Hai Quang Tran ,  Majid Aghababazadeh ,  M. Shane Bowen ,  Boyan Boyanov ,  Dale Buermann

IPC: B01L3/00 ,  F16K99/00 ,  C12Q1/6869 ,  B01L7/00 ,  G01N35/08

Abstract: Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.