利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

32 条记录 (耗时 0.043 秒)

    MULTI EPITOPE ASSAY
    21.
    发明申请
    MULTI EPITOPE ASSAY 有权
    多重抗生素测定

    公开(公告)号：US20130095495A1

    公开(公告)日：2013-04-18

    申请号：US13704258

    申请日：2011-06-14

    申请人： Gwenola Sabatte ,  Menno Willem Jose Prins ,  Toon Hendrik Evers ,  Wilhelmina Maria Hardeman ,  Joukje Garrelina Orsel

    发明人： Gwenola Sabatte ,  Menno Willem Jose Prins ,  Toon Hendrik Evers ,  Wilhelmina Maria Hardeman ,  Joukje Garrelina Orsel

    IPC分类号： G01N33/53

    CPC分类号： G01N33/5306 ,  G01N33/54306 ,  G01N33/54333

    摘要： The present invention is related to a method for detection of a biological target in an affinity assay, the method comprising the steps of providing a biological sample volume containing the biological target, adding a first capturing moiety to the biological sample volume comprising the biological target, wherein the first capturing moiety is adhered to a particle, concentration of the captured biological target into an elution volume that is smaller than the biological sample volume in step a), cleavage of the first capturing moiety or the biological target from the particle and direct or indirect detection and/or quantification of the biological target in a sandwich or competitive affinity assay format, wherein the biological target is associated with at least one capturing moiety, preferably at least two capturing moieties.

    摘要翻译： 本发明涉及亲和测定中生物靶标检测方法，该方法包括以下步骤：提供含生物靶的生物样品体积，向包含生物靶标的生物样品体积添加第一捕获部分， 其中所述第一捕获部分被粘附到颗粒上，所捕获的生物靶的浓度在步骤a）中小于生物样品体积的洗脱体积，从所述颗粒切割所述第一捕获部分或所述生物靶，并直接或 间接检测和/或以三明治或竞争亲和测定格式定量生物学靶标，其中生物靶标与至少一个捕获部分，优选至少两个捕获部分相关联。

    DETECTION OF MAGNETIC PARTICLES AND THEIR CLUSTERING
    22.
    发明申请
    DETECTION OF MAGNETIC PARTICLES AND THEIR CLUSTERING 审中-公开
    检测磁性颗粒及其聚集

    公开(公告)号：US20130088221A1

    公开(公告)日：2013-04-11

    申请号：US13705000

    申请日：2010-11-30

    申请人： Joannes Baptist Adrianus Dionisius Van Zon ,  Mkhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Mara Johanna Jacoba Sijbers

    发明人： Joannes Baptist Adrianus Dionisius Van Zon ,  Mkhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Mara Johanna Jacoba Sijbers

    IPC分类号： G01N27/72

    CPC分类号： G01N27/72 ,  B01L3/502761 ,  B82Y25/00 ,  G01N15/06 ,  G01N15/0656 ,  G01N2015/0693 ,  G01R33/1276 ,  H01F1/0036

    摘要： The invention relates to a method and associated apparatuses (100) for the detection of magnetic particles (MP) in a sample chamber (111). The method comprises the determination of a “particle-parameter” that is related to the amount of magnetic particles (MP) in a first detection region (P, C), the determination of a “cluster-parameter” that is related to the degree of clustering of magnetic particles (MP) in a second detection region (P, C), and the evaluation of the particle-parameter based on the cluster-parameter. Various apparatuses are disclosed that can be applied in said method. In one apparatus (100), a magnetic field (B) is generated in the sample chamber (111) in such a way that it has different inclinations in a first and second field region (P, C) and/or that it is oblique to the binding surface (112) in at least one field region. Magnetic particles (MP) are then detected in said first and second field region and/or in said at least one field region before and after a permanent switch-off of the inclined magnetic field. The resulting detection signals are related to each other to determine a cluster-parameter. In other embodiments, a cluster-parameter may be determined from light transmission measurements during the application of a magnetic field that is switched on and off.

    摘要翻译： 本发明涉及一种用于检测样品室（111）中的磁性颗粒（MP）的方法和相关装置（100）。 该方法包括确定与第一检测区域（P，C）中的磁性粒子（MP）的量相关的粒子参数，确定与磁性聚类程度相关的聚类参数 颗粒（MP）在第二检测区域（P，C）中，以及基于聚类参数的粒子参数的评估。 公开了可以应用于所述方法的各种装置。 在一个装置（100）中，在样品室（111）中产生磁场（B），使得其在第一和第二场区域（P，C）中具有不同的倾斜度和/或倾斜 到达至少一个场区域中的结合表面（112）。 然后在永久关闭倾斜磁场之前和之后，在所述第一和第二场区和/或所述至少一个场区中检测磁性颗粒（MP）。 所得到的检测信号彼此相关，以确定簇参数。 在其他实施例中，可以在施加接通和关断的磁场期间的光透射测量来确定簇参数。

    SENSING DEVICE FOR DETECTING A TARGET SUBSTANCE
    23.
    发明申请
    SENSING DEVICE FOR DETECTING A TARGET SUBSTANCE 有权
    用于检测目标物质的感应装置

    公开(公告)号：US20120040475A1

    公开(公告)日：2012-02-16

    申请号：US13201927

    申请日：2010-02-08

    申请人： Dominique Maria Bruls ,  Toon Hendrik Evers ,  Johannes Joseph Hubertina Barbara Schleipen

    发明人： Dominique Maria Bruls ,  Toon Hendrik Evers ,  Johannes Joseph Hubertina Barbara Schleipen

    IPC分类号： G01N21/55

    CPC分类号： G01N21/552 ,  G01N21/274 ,  G01N33/54373

    摘要： The present invention relates to a sensing device (100) for detecting a target substance (2) in an investigation region (113). The sensing device (100) comprises a sensing surface (112) with an investigation region (113) and a reference region (120) thereon. The sensing device (100) further comprises a reference element (121) located at the reference region (120). The reference element (121) is adapted to shield the reference region (120) from the target substance (2) such that light reflected at the reference region (120) under total internal reflection conditions remains unaffected by the presence or absence of the target substance (2). This allows measuring a property, typically the intensity, of light reflected at the reference region (120) independent of the presence or absence of the target substance (2). This measured property of the reflected light can be used for performing an improved correction of light reflected at the investigation region (113).

    摘要翻译： 本发明涉及一种用于在调查区域（113）中检测目标物质（2）的感测装置（100）。 感测装置（100）包括其上具有调查区域（113）和参考区域（120）的感测表面（112）。 感测装置（100）还包括位于参考区域（120）处的参考元件（121）。 参考元件（121）适于屏蔽参考区域（120）与目标物质（2），使得在全内反射条件下在参考区域（120）处反射的光线不会受到目标物质的存在或不存在的影响 （2）。 这允许独立于目标物质（2）的存在或不存在来测量在参考区域（120）处反射的光的特性（通常为强度）。 反射光的测量特性可用于对在调查区域（113）反射的光进行改进的校正。

    DETECTION OF TARGET COMPONENTS WITH THE HELP OF INDICATOR PARTICLES
    24.
    发明申请
    DETECTION OF TARGET COMPONENTS WITH THE HELP OF INDICATOR PARTICLES 有权
    用指示剂颗粒的帮助检测目标组分

    公开(公告)号：US20100330698A1

    公开(公告)日：2010-12-30

    申请号：US12863219

    申请日：2009-01-19

    申请人： Toon Hendrik Evers

    发明人： Toon Hendrik Evers

    IPC分类号： G01N33/50 ,  G01N33/48

    CPC分类号： G01N33/54373 ,  B82Y25/00 ,  B82Y30/00 ,  G01N21/552 ,  G01N27/745 ,  G01N33/542 ,  G01N33/54326

    摘要： The invention relates to a system and a method for the detection of target components (102) in a sample with the help of indicator particles (101) distributed in said sample. The distance (d) between indicator particles (101) and a contact surface (112) is determined after the target components could bind to the contact surface and/or the indicator particles. Thus it is possible to detect how many target components (102) are bound without a need for a binding between indicator particles (101) and contact surface (112). Optionally the indicator particles (101) can be affected by a modulated force, e.g. via an electromagnet (141). The determination of the distance (d) between indicator particles (101) and contact surface (112) may for example be achieved by frustrated total internal reflection, measurement of magnetic fields, or FRET.

    摘要翻译： 本发明涉及借助于分布在所述样品中的指示剂颗粒（101）来检测样品中的目标组分（102）的系统和方法。 在目标组分可以结合到接触表面和/或指示剂颗粒之后，确定指示剂颗粒（101）和接触表面（112）之间的距离（d）。 因此，可以检测多少目标部件（102）被绑定，而不需要指示剂颗粒（101）和接触表面（112）之间的结合。 任选地，指示剂颗粒（101）可以受到调制的力，例如， 经由电磁体（141）。 指示剂颗粒（101）和接触表面（112）之间的距离（d）的确定可以例如通过沮丧的全内反射，磁场测量或FRET来实现。

    EVALUATING ASSAYS WHICH OPTICAL INHOMOGENEITIES
    30.
    发明申请
    EVALUATING ASSAYS WHICH OPTICAL INHOMOGENEITIES 审中-公开
    评估光学不均匀性的测定

    公开(公告)号：US20140051187A1

    公开(公告)日：2014-02-20

    申请号：US14113964

    申请日：2012-04-23

    申请人： Toon Hendrik Evers ,  Derk Jan Wilfred Klunder ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adianus Dionisius Van Zon

    发明人： Toon Hendrik Evers ,  Derk Jan Wilfred Klunder ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adianus Dionisius Van Zon

    IPC分类号： G01N21/00

    摘要： The invention relates to a method and a sensor device (100) for evaluating an assay with a sample. During the assay, optical measurements are made at a sensing surface (112), and at least one “homogeneity-image” of the sensing surface (112) is generated. From this image, an “homogeneity-indicator” is determined for at least one region of interest, and the optical measurements are then evaluated in dependence on said indicator. The homogeneity-indicator may for example be a binary value which indicates if an inhomogeneity was detected or not. If an inhomogeneity was detected, all optical measurements may be rejected, only measurements for the involved region of interest may be rejected, or measurements for a selected sub-area of the involved region of interest(ROI) may be rejected.

    摘要翻译： 本发明涉及用于评价样品的测定方法和传感器装置（100）。 在测定期间，在感测表面（112）处进行光学测量，并且产生感测表面（112）的至少一个“均匀性图像”。 从该图像中，对于至少一个感兴趣的区域确定“均匀度指标”，然后根据所述指标来评估光学测量。 均匀度指示符可以例如是二进制值，其指示是否检测到不均匀性。 如果检测到不均匀性，则所有光学测量可能被拒绝，只有感兴趣区域的测量可能被拒绝，或者所涉及的感兴趣区域（ROI）的选定子区域的测量可能被拒绝。