公开(公告)号：US10466163B2

公开(公告)日：2019-11-05

申请号：US14113964

申请日：2012-04-23

申请人： Toon Hendrik Evers ,  Derk Jan Wilfred Klunder ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus Dionisius Van Zon

发明人： Toon Hendrik Evers ,  Derk Jan Wilfred Klunder ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus Dionisius Van Zon

IPC分类号： G01N33/53 ,  G01N21/00 ,  G01N21/552 ,  G01N21/88 ,  G01N21/94 ,  G06T7/00 ,  G01N33/52 ,  G01N21/15

摘要： During an assay, optical measurements are made at a sensing surface (112), and at least one “homogeneity-image” of the sensing surface (112) is generated. From this image, an “homogeneity-indicator” is determined for at least one region of interest, and the optical measurements are then evaluated in dependence on said indicator. The homogeneity-indicator may for example be a binary value which indicates if an inhomogeneity was detected or not. If an inhomogeneity was detected, all optical measurements may be rejected, only measurements for the involved region of interest may be rejected, or measurements for a selected sub-area of the involved region of interest (ROI) may be rejected.

公开(公告)号：US09772272B2

公开(公告)日：2017-09-26

申请号：US13498615

申请日：2010-09-24

申请人： Toon Hendrik Evers ,  Johannes Joseph Hubertina Barbara Schleipen ,  Joannes Baptist Adrianus Dionisius Van Zon ,  Derk Jan Wilfred Klunder ,  Josephus Arnoldus Hendricus Maria Kahlman ,  Ron Martinus Laurentius Van Lieshout ,  Mikhail Mikhaylovich Ovsyanko ,  Kim Van Ommering

发明人： Toon Hendrik Evers ,  Johannes Joseph Hubertina Barbara Schleipen ,  Joannes Baptist Adrianus Dionisius Van Zon ,  Derk Jan Wilfred Klunder ,  Josephus Arnoldus Hendricus Maria Kahlman ,  Ron Martinus Laurentius Van Lieshout ,  Mikhail Mikhaylovich Ovsyanko ,  Kim Van Ommering

IPC分类号： G01N15/06 ,  G01N15/14 ,  G01N21/47 ,  G01N21/552 ,  G01N21/64 ,  G01N27/74 ,  G01N33/543 ,  G01N15/02 ,  G01N35/00

CPC分类号： G01N15/0612 ,  G01N15/1434 ,  G01N21/47 ,  G01N21/552 ,  G01N21/648 ,  G01N27/745 ,  G01N33/54333 ,  G01N35/0098 ,  G01N2015/025 ,  G01N2201/0627

摘要： A substance determining apparatus determines a substance within a fluid where particles, which have attached the substance, are bound to a binding surface. A sensing unit is configured to generate a sensing signal being indicative of at least one of i) a distance between the particles bound on the binding surface and the binding surface, and ii) an in-plane position of the particles bound on the binding surface. A binding discrimination unit is configured to discriminate between different kinds of binding of the particles bound on the binding surface depending on the generated sensing signal. The binding discrimination unit may be a unit for determining the part of the sensing signal being caused by specifically bound particles and for determining the substance based on this determined part of the sensing signal.

公开(公告)号：US09339813B2

公开(公告)日：2016-05-17

申请号：US13516060

申请日：2010-12-10

申请人： Josephus Arnoldus Henricus Maria Kahlman ,  Joannes Baptist Adrianus Dionisius Van Zon ,  Johannes Joseph Hubertina Barbara Schleipen ,  Derk Jan Wilfred Klunder ,  Toon Hendrik Evers ,  Ron Martinus Laurentius Van Lieshout

发明人： Josephus Arnoldus Henricus Maria Kahlman ,  Joannes Baptist Adrianus Dionisius Van Zon ,  Johannes Joseph Hubertina Barbara Schleipen ,  Derk Jan Wilfred Klunder ,  Toon Hendrik Evers ,  Ron Martinus Laurentius Van Lieshout

IPC分类号： G01N21/17 ,  B01L3/00 ,  G01N21/552 ,  G01N21/84 ,  G01N33/543

CPC分类号： B01L3/5027 ,  B01L2200/0605 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2400/0406 ,  G01N21/552 ,  G01N21/8483 ,  G01N33/54373

摘要： The invention relates to a substance determining apparatus for determining a substance within a fluid. Particles attach the substance and bind to a binding surface (30), wherein a sensing signal is generated depending on the bound particles. Binding events indicating a binding of a particle on the binding surface (30) are determined from the generated sensing signal, and the substance within the fluid is determined based on the determined binding events. During a procedure of determining a substance within a fluid, particles may bind to the binding surface and may leave the binding surface. Therefore, during this procedure a number of binding events can be determined being much larger than the number of bound particles. The determination of the substance within the fluid can therefore be based on a very large amount of data, thereby increasing the accuracy of determining the substance within the fluid.

摘要翻译： 本发明涉及一种用于确定流体内物质的物质确定装置。 颗粒附着物质并结合到结合表面（30），其中根据结合的颗粒产生感测信号。 根据产生的感测信号确定指示结合表面（30）上的颗粒结合的结合事件，并且基于所确定的结合事件确定流体内的物质。 在确定流体内的物质的过程中，颗粒可能结合到结合表面并且可能离开结合表面。 因此，在此过程中，可以确定许多结合事件比结合粒子的数量大得多。 因此，流体内的物质的测定可以基于非常大量的数据，从而提高确定流体内物质的准确性。

公开(公告)号：US20120329039A1

公开(公告)日：2012-12-27

申请号：US13516060

申请日：2010-12-10

申请人： Josephus Arnoldus Henricus Maria Kahlman ,  Joannes Baptist Adrianus Dionisius Van Zon ,  Johannes Joseph Hubertina Barbara Schleipen ,  Derk Jan Wilfred Klunder ,  Toon Hendrik Evers ,  Ron Martinus Laurentius Van Lieshout

发明人： Josephus Arnoldus Henricus Maria Kahlman ,  Joannes Baptist Adrianus Dionisius Van Zon ,  Johannes Joseph Hubertina Barbara Schleipen ,  Derk Jan Wilfred Klunder ,  Toon Hendrik Evers ,  Ron Martinus Laurentius Van Lieshout

IPC分类号： G01N33/566 ,  C12M1/34 ,  C12Q1/70 ,  G01N30/00 ,  G01N33/53

CPC分类号： B01L3/5027 ,  B01L2200/0605 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2400/0406 ,  G01N21/552 ,  G01N21/8483 ,  G01N33/54373

摘要： The invention relates to a substance determining apparatus for determining a substance within a fluid. Particles attach the substance and bind to a binding surface (30), wherein a sensing signal is generated depending on the bound particles. Binding events indicating a binding of a particle on the binding surface (30) are determined from the generated sensing signal, and the substance within the fluid is determined based on the determined binding events. During a procedure of determining a substance within a fluid, particles may bind to the binding surface and may leave the binding surface. Therefore, during this procedure a number of binding events can be determined being much larger than the number of bound particles. The determination of the substance within the fluid can therefore be based on a very large amount of data, thereby increasing the accuracy of determining the substance within the fluid.

摘要翻译： 本发明涉及一种用于确定流体内物质的物质确定装置。 颗粒附着物质并结合到结合表面（30），其中根据结合的颗粒产生感测信号。 根据产生的感测信号确定指示结合表面（30）上的颗粒结合的结合事件，并且基于所确定的结合事件确定流体内的物质。 在确定流体内的物质的过程中，颗粒可能结合到结合表面并且可能离开结合表面。 因此，在此过程中，可以确定许多结合事件比结合粒子的数量大得多。 因此，流体内的物质的测定可以基于非常大量的数据，从而提高确定流体内物质的准确性。

公开(公告)号：US20120202194A1

公开(公告)日：2012-08-09

申请号：US13498615

申请日：2010-09-24

申请人： Toon Hendrik Evers ,  Johannes Joseph Hubertina Barbara Schleipen ,  Joannes Baptist Adrianus Dionisius Van Zon ,  Derk Jan Wilfred Klunder ,  Josephus Arnoldus Hendricus Maria Kahlman ,  Ron Martinus Laurentius Van Lieshout ,  Mikhail Mikhaylovich Ovsyanko ,  Kim Van Ommering

发明人： Toon Hendrik Evers ,  Johannes Joseph Hubertina Barbara Schleipen ,  Joannes Baptist Adrianus Dionisius Van Zon ,  Derk Jan Wilfred Klunder ,  Josephus Arnoldus Hendricus Maria Kahlman ,  Ron Martinus Laurentius Van Lieshout ,  Mikhail Mikhaylovich Ovsyanko ,  Kim Van Ommering

IPC分类号： G01N21/63 ,  C12M1/34 ,  C12Q1/02 ,  C12Q1/70 ,  G01N33/53 ,  G01N21/64

CPC分类号： G01N15/0612 ,  G01N15/1434 ,  G01N21/47 ,  G01N21/552 ,  G01N21/648 ,  G01N27/745 ,  G01N33/54333 ,  G01N35/0098 ,  G01N2015/025 ,  G01N2201/0627

摘要： The invention relates to a substance determining apparatus for determining a substance within a fluid. Particles, which have attached the substance, are bound to a binding surface (30). A sensing unit (33) is adapted to generate a sensing signal being indicative of at least one of i) a distance between the particles bound on the binding surface (30) and the binding surface (30) and ii) an in-plane position of the particles bound on the binding surface. A binding discrimination unit (34) is adapted to discriminate between different kinds of binding of the particles bound on the binding surface (30) depending on the generated sensing signal. The binding discrimination unit (34) is preferentially a unit for determining the part of the sensing signal being caused by specifically bound particles and to determine the substance based on this determined part of the sensing signal.

摘要翻译： 本发明涉及一种用于确定流体内物质的物质确定装置。 具有附着物质的颗粒结合至结合表面（30）。 感测单元（33）适于产生感测信号，该感测信号指示i）结合在装订表面（30）和装订表面（30）上的颗粒之间的距离和ii）平面内位置 的结合表面上的颗粒。 绑定识别单元（34）适于根据产生的感测信号来区分绑定在绑定表面（30）上的粒子的不同种类的绑定。 装订识别单元（34）优选地是用于确定由特定结合的粒子引起的感测信号的部分的单元，并且基于该确定的感测信号的部分来确定物质。

公开(公告)号：US09612239B2

公开(公告)日：2017-04-04

申请号：US14001566

申请日：2012-02-27

申请人： Joannes Baptist Adrianus Dionisius Van Zon ,  Toon Hendrik Evers ,  Ron Martinus Laurentius Van Lieshout ,  Wilhelmina Maria Hardeman ,  Derk Jan Wilfred Klunder

发明人： Joannes Baptist Adrianus Dionisius Van Zon ,  Toon Hendrik Evers ,  Ron Martinus Laurentius Van Lieshout ,  Wilhelmina Maria Hardeman ,  Derk Jan Wilfred Klunder

IPC分类号： G01N21/17 ,  G01N33/543 ,  G01N21/552 ,  G01N21/64 ,  G01N21/77 ,  G01N27/74

CPC分类号： G01N33/54326 ,  G01N21/552 ,  G01N21/648 ,  G01N21/7703 ,  G01N27/745

摘要： The invention relates to a substance determining apparatus (19) for determining a substance within a fluid. A location event determination unit (34) determines location events indicating that a particle, which may have attached the substance, is located in a sensing region and a property of the location events from the sensing signal generated by sensing the sensing region. A location events selection unit (70) selects a part of the location events having a property in a predefined property range, and a substance determination unit (40) determines the substance within the fluid (3) based on the selected part of the location events. This allows tuning the determination of the substance within the fluid to a desired property range, which can relate to a desired kind of bound particles, thereby reducing the influence of unwanted effects on the determination of the substance and, thus, improving the determination accuracy.

公开(公告)号：US09075052B2

公开(公告)日：2015-07-07

申请号：US13498482

申请日：2010-09-23

申请人： Johannes Joseph Hubertina Barbara Schleipen ,  Joannes Baptist Adrianus Dionisius Van Zon ,  Derk Jan Wilfred Klunder ,  Toon Hendrik Evers ,  Josephus Arnoldus Henricus Maria Kahlman ,  Ron Martinus Laurentius Van Lieshout ,  Mikhail Mikhaylovich Ovsyanko

发明人： Johannes Joseph Hubertina Barbara Schleipen ,  Joannes Baptist Adrianus Dionisius Van Zon ,  Derk Jan Wilfred Klunder ,  Toon Hendrik Evers ,  Josephus Arnoldus Henricus Maria Kahlman ,  Ron Martinus Laurentius Van Lieshout ,  Mikhail Mikhaylovich Ovsyanko

IPC分类号： G01N21/00 ,  G01N33/543 ,  G01N21/47 ,  G01N21/552 ,  G01N21/64 ,  G01R33/12 ,  G01N21/51 ,  G01N21/49 ,  G01N27/72 ,  G01N15/10 ,  G01N15/00

CPC分类号： G01N33/54373 ,  G01N15/10 ,  G01N15/1031 ,  G01N21/47 ,  G01N21/49 ,  G01N21/51 ,  G01N21/552 ,  G01N21/6458 ,  G01N27/72 ,  G01N33/54313 ,  G01N2015/0065 ,  G01N2201/12 ,  G01R33/1269

摘要： A biosensor system for the detection of particles includes a biosensor cartridge having a sensor surface. A biosensor magnet assembly is disposed on one side of the cartridge for generating a magnetic field effective at the cartridge and the sensor surface. The biosensor magnet assembly includes at least two magnetic sub-units separated by a gap. A first optical detection system detects the particles arranged at the same side of the cartridge as the magnet assembly. The magnet assembly and the first optical sensor are disposed such that the optical detection is accomplished through the gap of the magnet assembly.

摘要翻译： 用于检测颗粒的生物传感器系统包括具有传感器表面的生物传感器盒。 生物传感器磁体组件设置在盒的一侧，用于在盒和传感器表面产生有效的磁场。 生物传感器磁体组件包括由间隙分开的至少两个磁性子单元。 第一光学检测系统检测与磁体组件布置在盒的同一侧的颗粒。 磁体组件和第一光学传感器被布置成使得通过磁体组件的间隙实现光学检测。

公开(公告)号：US20120250005A1

公开(公告)日：2012-10-04

申请号：US13498482

申请日：2010-09-23

申请人： Johannes Joseph Hubertina Barbara Schleipen ,  Joannes Baptist Adrianus Dionisius Van Zon ,  Derk Jan Wilfred Klunder ,  Toon Hendrik Evers ,  Josephus Arnoldus Henricus Maria Kahlman ,  Ron Martinus Laurentius Van Lieshout ,  Mikhail Mikhaylovich Ovsyanko

发明人： Johannes Joseph Hubertina Barbara Schleipen ,  Joannes Baptist Adrianus Dionisius Van Zon ,  Derk Jan Wilfred Klunder ,  Toon Hendrik Evers ,  Josephus Arnoldus Henricus Maria Kahlman ,  Ron Martinus Laurentius Van Lieshout ,  Mikhail Mikhaylovich Ovsyanko

IPC分类号： G01N21/49 ,  G01R33/12

CPC分类号： G01N33/54373 ,  G01N15/10 ,  G01N15/1031 ,  G01N21/47 ,  G01N21/49 ,  G01N21/51 ,  G01N21/552 ,  G01N21/6458 ,  G01N27/72 ,  G01N33/54313 ,  G01N2015/0065 ,  G01N2201/12 ,  G01R33/1269

摘要： The present invention is related to a biosensor system for the detection of particles comprising: a biosensor cartridge comprising a sensor surface, a biosensor magnet assembly on one side of the cartridge for generating a magnetic field effective at the cartridge and the sensor surface, the first biosensor magnet assembly comprising at least two magnetic sub-units separated by a gap, a first optical detection system for detecting the particles arranged at the same side of the cartridge as the magnet assembly, whereas the magnet assembly is designed in a way that the optical detection is accomplished through the gap of the magnet assembly.

摘要翻译： 本发明涉及一种用于检测颗粒的生物传感器系统，包括：生物传感器盒，包括传感器表面，在盒的一侧上的生物传感器磁体组件，用于产生在盒和感测器表面有效的磁场;第一 生物传感器磁体组件，其包括由间隙分隔开的至少两个磁性子单元;第一光学检测系统，用于检测与磁体组件位于盒的同一侧的颗粒，而磁体组件设计成使得光学 通过磁体组件的间隙实现检测。

公开(公告)号：US20140057366A1

公开(公告)日：2014-02-27

申请号：US13988833

申请日：2011-11-28

申请人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus dionisius Van Zon

发明人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus dionisius Van Zon

IPC分类号： G01N33/543

CPC分类号： G01N33/54373 ,  G01N21/274 ,  G01N21/552 ,  G01N27/72 ,  G01N27/745 ,  G01N27/825 ,  G01N33/54326 ,  G01N35/0098

摘要： The invention relates to a sensor device (100) and a method for the detection of magnetic particles (1) in a sample chamber (2) with a contact surface (11). The sensor device (100) comprises a sensor unit (120, 130) for detecting magnetic particles (1) in a target region (TR) and/or in at least one reference region on the contact surface. Moreover, it comprises a magnetic field generator (140) for generating a magnetic field that shall guide magnetic particles to the contact surface. With the help of these components, an “auxiliary parameter” is determined that is related to the magnetic particles (1) and/or their movement but that is independent of binding processes taking place in the target region between magnetic particles and the contact surface. The auxiliary parameter may for example be related to the degree of mismatch between the positions reached by the magnetic particles (1) under the influence of a magnetic field and the target region (TR). The evaluation results can be used to validate and/or correct the measurements obtained in the target region (TR).

摘要翻译： 本发明涉及一种传感器装置（100）和用于在具有接触表面（11））的样品室（2）中检测磁性颗粒（1）的方法。 传感器装置（100）包括用于检测目标区域（TR）中和/或接触表面上的至少一个参考区域中的磁性颗粒（1）的传感器单元（120,130）。 此外，它包括用于产生磁场的磁场发生器（140），该磁场将磁性颗粒引导到接触表面。 在这些组件的帮助下，确定与磁性颗粒（1）和/或它们的运动相关的“辅助参数”，但是与磁性颗粒和接触表面之间的目标区域中发生的结合过程无关。 辅助参数可以例如与在磁场和目标区域（TR）的影响下由磁性颗粒（1）达到的位置之间的失配程度相关。 评估结果可用于验证和/或校正在目标区域（TR）中获得的测量结果。

公开(公告)号：US09841421B2

公开(公告)日：2017-12-12

申请号：US13988833

申请日：2011-11-28

申请人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus Dionisius Van Zon

发明人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus Dionisius Van Zon

IPC分类号： G01N33/543 ,  G01N27/82 ,  G01N27/72 ,  G01N27/74 ,  G01N35/00 ,  G01N21/27 ,  G01N21/552

CPC分类号： G01N33/54373 ,  G01N21/274 ,  G01N21/552 ,  G01N27/72 ,  G01N27/745 ,  G01N27/825 ,  G01N33/54326 ,  G01N35/0098

摘要： The invention relates to a sensor device (100) and a method for the detection of magnetic particles (1) in a sample chamber (2) with a contact surface (11). The sensor device (100) comprises a sensor unit (120, 130) for detecting magnetic particles (1) in a target region (TR) and/or in at least one reference region on the contact surface. Moreover, it comprises a magnetic field generator (140) for generating a magnetic field that shall guide magnetic particles to the contact surface. With the help of these components, an “auxiliary parameter” is determined that is related to the magnetic particles (1) and/or their movement but that is independent of binding processes taking place in the target region between magnetic particles and the contact surface. The auxiliary parameter may for example be related to the degree of mismatch between the positions reached by the magnetic particles (1) under the influence of a magnetic field and the target region (TR). The evaluation results can be used to validate and/or correct the measurements obtained in the target region (TR).