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21.发明授权公开(公告)号：US07932071B2
公开(公告)日：2011-04-26
申请号：US12493428
申请日：2009-06-29
CPC分类号： C12N9/22
摘要： A polypeptide having an RNaseH activity from Archaeoglobus profundus and being highly useful in gene engineering; a gene encoding this polypeptide; and a genetic engineering process for producing the polypeptide.
摘要翻译： 具有来自古细菌球菌的RNaseH活性并且在基因工程中非常有用的多肽; 编码该多肽的基因; 以及用于产生多肽的遗传工程方法。
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公开(公告)号：US20090269809A1
公开(公告)日：2009-10-29
申请号：US12493428
申请日：2009-06-29
CPC分类号： C12N9/22
摘要： A polypeptide having an RNaseH activity and being highly useful in gene engineering; a gene encoding this polypeptide; and a genetic engineering process for producing the polypeptide.
摘要翻译： 具有RNaseH活性并且在基因工程中非常有用的多肽; 编码该多肽的基因; 以及用于产生多肽的遗传工程方法。
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公开(公告)号：US20070037247A1
公开(公告)日：2007-02-15
申请号：US10526073
申请日：2003-08-26
CPC分类号： C12N9/22
摘要： A polypeptide having an RNaseH activity and being highly useful in gene engineering; a gene encoding. this polypeptide; and a genetic engineering process for producing the polypeptide.
摘要翻译： 具有RNaseH活性并且在基因工程中非常有用的多肽; 一个基因编码。 该多肽; 以及用于产生多肽的遗传工程方法。
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公开(公告)号：US20070298415A1
公开(公告)日：2007-12-27
申请号：US10582345
申请日：2004-12-06
申请人： Takashi Uemori , Osamu Takeda , Junko Yamamoto , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Osamu Takeda , Junko Yamamoto , Hiroyuki Mukai , Kiyozo Asada , Ikunoshin Kato
CPC分类号： C12Q1/6844 , C12Q2531/119 , C12Q2525/185 , C12Q2537/143 , C12Q2525/161
摘要： In the case where a template nucleic acid has sequences substantially the same as each other, a primer is designed in a region between these sequences so as to give a product in a ladder structure in which a target region is polymerized in a single sequence via the same sequences in the ICAN reaction. By using a chimeric oligonucleotide primer and a ladder-forming oligonucleotide primer containing a specific base sequence, an amplification product having a ladder structure can be positively formed and thus the sensitivity, amplification efficiency and reaction speed in the ICAN reaction can be elevated.
摘要翻译： 在模板核酸具有彼此基本相同的序列的情况下，在这些序列之间的区域中设计引物，以便产生梯形结构中的产物，其中靶区域通过经由 在ICAN反应中相同的序列。 通过使用嵌合寡核苷酸引物和含有特定碱基序列的梯形寡核苷酸引物，可以积极地形成具有梯形结构的扩增产物，从而可以提高ICAN反应中的灵敏度，扩增效率和反应速度。
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公开(公告)号：US20110151467A1
公开(公告)日：2011-06-23
申请号：US13059703
申请日：2009-08-31
申请人： Kanako Usui , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
发明人： Kanako Usui , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
CPC分类号： C12N15/1096 , C12Q1/6848 , C12Q2563/179 , C12Q2561/113 , C12Q2521/301 , C12Q2521/107
摘要： A composition for use in amplifying cDNA synthesized by a reverse transcription reaction and detecting RNA that serves as a template of the reverse transcription reaction, the composition containing a thermostable DNA polymerase, a thermostable ribonuclease H, and an intercalating dye. Since the composition of the present invention can suppress the influences to the nucleic acid amplification reaction by RNA that serves as a template for cDNA synthesis, the composition is useful in the detection of RNA, and more useful in quantification of RNA having a desired sequence by real-time RT-PCR.
摘要翻译： 用于扩增通过逆转录反应合成的cDNA并检测作为逆转录反应的模板的RNA的组合物，所述组合物含有热稳定的DNA聚合酶，热稳定性核糖核酸酶H和嵌入染料。 由于本发明的组合物可以抑制用作cDNA合成模板的RNA对核酸扩增反应的影响，因此该组合物可用于RNA的检测，并且更有用于定量具有所需序列的RNA 实时RT-PCR。
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公开(公告)号：US5366883A
公开(公告)日：1994-11-22
申请号：US894212
申请日：1992-06-09
申请人： Kiyozo Asada , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato , Kenneth Laderman , Christian B. Anfinsen
发明人： Kiyozo Asada , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato , Kenneth Laderman , Christian B. Anfinsen
IPC分类号： C07K14/195 , C12N1/21 , C12N9/26 , C12N9/28 , C12N15/09 , C12N15/56 , C12R1/125 , C12R1/19 , C12N15/63 , C12N15/75
CPC分类号： C12N9/2417 , C07K14/195
摘要： The present invention relates, in general, to a cloned .alpha.-amylase gene, and, in particular, to a cloned hyperthermophilic .alpha.-amylase gene and to methods of producing .alpha.-amylase using same.
摘要翻译： 本发明一般涉及克隆的α-淀粉酶基因，特别是涉及克隆的超嗜热α-淀粉酶基因以及使用其生产α-淀粉酶的方法。
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公开(公告)号：US20090098600A1
公开(公告)日：2009-04-16
申请号：US12194364
申请日：2008-08-19
申请人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kikyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kikyozo Asada , Ikunoshin Kato
CPC分类号： C12N9/22
摘要： Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.
摘要翻译： 具有在遗传工程中非常有用的RNase H活性的多肽; 编码这些多肽的基因; 以及用于遗传工程化生产这些多肽的方法。
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28.发明申请Method of stabilizing reagent for amplifying or detecting nucleic acid and storage method 审中-公开稳定核酸扩增或检测试剂的方法及储存方法公开(公告)号：US20050059000A1
公开(公告)日：2005-03-17
申请号：US10478633
申请日：2002-06-12
申请人： Hiroaki Sagawa , Takashi Uemori , Hiroyuki Mukai , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Kiyozo Asada , Ikunoshin Kato
发明人： Hiroaki Sagawa , Takashi Uemori , Hiroyuki Mukai , Junko Yamamoto , Jun Tomono , Eiji Kobayashi , Tatsuji Enoki , Kiyozo Asada , Ikunoshin Kato
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q2521/327 , C12Q2525/121 , C12Q2527/137
摘要： A method of stabilizing a reaction reagent for highly sensitively and specifically amplifying a target nucleic acid in a sample with the use of a chimeric oligonucleotide primer and a method of storing the same over a long time; and a method of highly sensitively detecting a pathogenic microorganism and a virus.
摘要翻译： 使用嵌合寡核苷酸引物对样品中的靶核酸进行高灵敏度和特异性扩增的反应试剂的稳定化方法及其长时间存储的方法; 以及高敏感性检测病原微生物和病毒的方法。
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公开(公告)号：US07422888B2
公开(公告)日：2008-09-09
申请号：US10380430
申请日：2001-09-13
申请人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kiyozo Asada , Ikunoshin Kato
发明人： Takashi Uemori , Yoshimi Sato , Nobuto Koyama , Ryo Hirano , Hikaru Takakura , Hiroshi Kobori , Yuji Hashimoto , Kiyozo Asada , Ikunoshin Kato
CPC分类号： C12N9/22
摘要： Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.
摘要翻译： 具有在遗传工程中非常有用的RNase H活性的多肽; 编码这些多肽的基因; 以及用于遗传工程化生产这些多肽的方法。
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公开(公告)号：US10760074B2
公开(公告)日：2020-09-01
申请号：US13059703
申请日：2009-08-31
申请人： Kanako Usui , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
发明人： Kanako Usui , Takashi Uemori , Hiroyuki Mukai , Ikunoshin Kato
IPC分类号： C12Q1/68 , C12N15/10 , C12Q1/6848
摘要： A composition for use in amplifying cDNA synthesized by a reverse transcription reaction and detecting RNA that serves as a template of the reverse transcription reaction, the composition containing a thermostable DNA polymerase, a thermostable ribonuclease H, and an intercalating dye. Since the composition of the present invention can suppress the influences to the nucleic acid amplification reaction by RNA that serves as a template for cDNA synthesis, the composition is useful in the detection of RNA, and more useful in quantification of RNA having a desired sequence by real-time RT-PCR.
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