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    Variants of beta-glucosidases
    21.
    发明申请
    Variants of beta-glucosidases 有权
    β-葡糖苷酶的变体

    公开(公告)号：US20040253702A1

    公开(公告)日：2004-12-16

    申请号：US10836063

    申请日：2004-04-30

    申请人： Novozymes Biotech, Inc.

    发明人： Ana Fidantsef ,  Michael Lamsa ,  Brian Gorre Clancy

    IPC分类号： C12N009/42 ,  C12Q001/68 ,  C07H021/04 ,  C12N001/21 ,  C11D003/386

    CPC分类号： C12N9/2445 ,  A61K36/062 ,  C12P19/02 ,  C12P19/14 ,  C12Q1/6895 ,  C12Y302/01021

    摘要： The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

    摘要翻译： 本发明涉及亲本β-葡糖苷酶的变体，其包含在对应于SEQ ID NO：2的氨基酸1至842的位置142,183,266和703的一个或多个位置处的取代或对应于位置142， SEQ ID NO：70的氨基酸1至844的183,266和705，其中所述变体具有β-葡糖苷酶活性。 本发明还涉及编码变体β-葡糖苷酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

    Methods for modifying the production of a polypeptide
    22.
    发明申请
    Methods for modifying the production of a polypeptide 审中-公开
    修饰多肽生产的方法

    公开(公告)号：US20040197854A1

    公开(公告)日：2004-10-07

    申请号：US10000845

    申请日：2001-10-24

    申请人： Novozymes Biotech, Inc. ,  Novozymes A/S

    发明人： Howard Brody ,  Debbie S. Yaver ,  Michael H. Lamsa ,  Kim Hansen

    IPC分类号： C12P021/02 ,  C12N005/06

    CPC分类号： C12N9/2434 ,  C12N9/20 ,  C12N15/67 ,  C12N15/80 ,  C12P21/02 ,  C12Y302/01004

    摘要： The present invention relates to methods for modifying the production of a polypeptide, comprising: (a) introducing a nucleic acid construct into a cell, wherein the cell comprises a DNA sequence encoding a polypeptide, under conditions in which the nucleic acid construct integrates into the genome of the cell at a locus not within the DNA sequence encoding the polypeptide to produce a mutant cell, wherein the integration of the nucleic acid construct modifies the production of the polypeptide by the mutant cell relative to the cell when the mutant cell and the cell are cultured under the same conditions; and (b) identifying the mutant cell with the modified production of the polypeptide.

    摘要翻译： 本发明涉及用于修饰多肽生产的方法，包括：（a）将核酸构建体导入细胞，其中所述细胞包含编码多肽的DNA序列，其中所述核酸构建体整合到 在编码多肽的DNA序列内的不在编码突变细胞的DNA序列中的基因座的细胞的基因组，其中当突变细胞和细胞时，核酸构建体的整合修饰了突变细胞相对于细胞的多肽的产生 在相同条件下培养; 和（b）鉴定具有多肽修饰产生的突变细胞。

    Method for preparing polypeptide variants
    23.
    发明申请
    Method for preparing polypeptide variants 审中-公开
    制备多肽变体的方法

    公开(公告)号：US20030199038A1

    公开(公告)日：2003-10-23

    申请号：US10422013

    申请日：2003-04-22

    申请人： Novozymes Biotech, Inc.

    发明人： Howard Brody ,  Suzanne M. Otani ,  Joel R. Cherry

    IPC分类号： C12P021/02 ,  C12N009/02 ,  C12N009/10 ,  C12N009/16 ,  C12N009/88 ,  C12N009/90 ,  C12N001/16 ,  C12N015/74

    CPC分类号： C12N15/1082 ,  C12N15/102 ,  C12N15/1034

    摘要： The present invention relates to methods for preparing variants of a nucleotide sequence, comprising: (a) introducing into a population of filamentous fungal host cells: (i) one or more circular plasmids comprising a DNA sequence and a plasmid replicator mediating autonomous replication, wherein the one or more circularized plasmids are linearized by digestion of the DNA sequence and removal of a portion of the DNA sequence; and (ii) a library of DNA fragments comprising one or more mutations of the DNA sequence, wherein the fragments comprise at least two regions, one or more regions which are homologous to the 5null region or the 3null region of the gap in the linearized DNA sequence and/or plasmid sequence and one or more second regions which are homologous to the 5null region or the 3null region of the DNA fragments of the library; wherein the linearized plasmids and the DNA fragments recombine by in vivo recombination to produce a plurality of autonomously replicating plasmids comprising one or more variants of the DNA sequence; (b) cultivating the population of recombinant filamentous fungal cells in a medium suitable for growth; and (c) screening the population of recombinant filamentous fungal cells for variants of the DNA sequence contained on one or more autonomously replicating circularized plasmids.

    摘要翻译： 本发明涉及用于制备核苷酸序列变体的方法，包括：（a）将丝状真菌宿主细胞群引入：（i）一种或多种包含DNA序列的环状质粒和介导自主复制的质粒复制子，其中 一个或多个环化质粒通过消化DNA序列并去除部分DNA序列而线性化; 和（ii）包含DNA序列的一个或多个突变的DNA片段的文库，其中所述片段包含至少两个区域，与所述DNA序列中的间隙的5'区域或3'区域同源的一个或多个区域 线性化DNA序列和/或质粒序列以及与文库DNA片段的5'区域或3'区域同源的一个或多个第二区域; 其中线性化质粒和DNA片段通过体内重组重组以产生包含DNA序列的一个或多个变体的多个自主复制质粒; （b）在适合生长的培养基中培养重组丝状真菌细胞群; 和（c）筛选重组丝状真菌细胞群体用于包含在一个或多个自主复制的环化质粒上的DNA序列的变体。

    Methods for producing a polypeptide in a bacillus cell
    24.
    发明申请
    Methods for producing a polypeptide in a bacillus cell 有权
    在芽孢杆菌细胞中产生多肽的方法

    公开(公告)号：US20030170876A1

    公开(公告)日：2003-09-11

    申请号：US09834271

    申请日：2001-04-12

    申请人： Novozymes Biotech, Inc.

    发明人： William Widner ,  Alan Sloma ,  Michael D. Thomas

    IPC分类号： C12N001/20

    CPC分类号： C12N15/75

    摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a nullconsensusnull promoter having the sequence TTGACA for the nullnull35null region and TATAAT for the nullnull10null region operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and (ii) an mRNA processing/stabilizing sequence located downstream of the nullconsensusnull promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.

    摘要翻译： 本发明涉及产生多肽的方法，其包括：（a）在有助于产生多肽的培养基中培养芽孢杆菌宿主细胞，其中所述芽孢杆菌细胞包含核酸构建体，其包含（i）串联启动子，其中 串联启动子的每个启动子序列可操作地连接到编码多肽的核酸序列的单拷贝，或者还有（ii）位于串联启动子下游的mRNA加工/稳定序列和编码多肽的核酸序列的上游 ; 和（b）从培养基中分离多肽。 本发明还涉及产生多肽的方法，其包括：（a）在有助于产生多肽的培养基中培养芽孢杆菌宿主细胞，其中所述芽孢杆菌细胞包含核酸构建体，其包含（i）“共有” 具有“-35”区域的TTGACA序列的启动子和“-10”区域的TATAAT可操作地连接到编码多肽的核酸序列的单拷贝，和（ii）位于“ 共有“启动子和编码多肽的核酸序列的上游; 和（b）从培养基中分离多肽。

    Methods for producing potato products
    26.
    发明申请
    Methods for producing potato products 失效
    马铃薯产品的生产方法

    公开(公告)号：US20020004085A1

    公开(公告)日：2002-01-10

    申请号：US09834560

    申请日：2001-04-13

    申请人： Novozymes Biotech, Inc.

    发明人： Feng Xu ,  Lene Venke Kofod ,  Hans Sejr Olsen

    IPC分类号： A23L001/216

    CPC分类号： C12Y101/03004 ,  A23L19/18 ,  A23L19/19 ,  C12Y110/03002 ,  C12Y203/02013 ,  C12Y301/01 ,  C12Y301/01003 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01015 ,  C12Y302/01133 ,  C12Y304/00

    摘要： The present invention relates to methods for producing consumable products from potatoes, comprising: (a) treating a potato substance with an effective amount of one or more exogenous enzymes selected from the group consisting of an amyloglucosidase, glucose oxidase, laccase, lipase, maltogenic amylase, pectinase, pentosanase, protease, and transglutaminase, and (b) processing the enzyme-treated potato substance to produce a potato product. The invention also relates to consumable products obtained from potatoes by the methods of the present invention.

    摘要翻译： 本发明涉及从马铃薯生产易消耗品的方法，包括：（a）用有效量的一种或多种外源酶处理马铃薯物质，所述外源酶选自淀粉葡糖苷酶，葡萄糖氧化酶，漆酶，脂肪酶，麦芽糖淀粉酶 ，果胶酶，戊聚糖酶，蛋白酶和转谷氨酰胺酶，和（b）加工经酶处理的马铃薯物质以产生马铃薯产品。 本发明还涉及通过本发明的方法从马铃薯获得的消耗品。

    Methods for monitoring multiple gene expression
    29.
    发明申请
    Methods for monitoring multiple gene expression 有权
    多基因表达监测方法

    公开(公告)号：US20040229367A1

    公开(公告)日：2004-11-18

    申请号：US10653047

    申请日：2003-08-29

    申请人： Novozymes Biotech, Inc. ,  Novozymes A/S, Inc.

    发明人： Randy M. Berka ,  Michael W. Rey ,  Jeffrey R. Shuster ,  Sakari Kauppinen ,  Ib Groth Clausen ,  Peter Bjarke Olsen

    IPC分类号： C12N015/74 ,  C12N001/16

    CPC分类号： C12Q1/6895 ,  C07K14/37 ,  C12Q1/6837 ,  C12Q2600/158

    摘要： The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing filamentous fungal expressed sequenced tags. The present invention also relates to filamentous fungal expressed sequenced tags and to computer readable media and substrates containing such expressed sequenced tags for monitoring expression of a plurality of genes in filamentous fungal cells.

    摘要翻译： 本发明涉及用于使用含有丝状真菌表达的测序标签的微阵列来监测第一丝状真菌细胞中多个基因的差异表达相对于一个或多个第二丝状真菌细胞中相同基因表达的差异表达的方法。 本发明还涉及丝状真菌表达的测序标签以及包含用于监测丝状真菌细胞中多种基因的表达的表达的测序标签的计算机可读介质和底物。

    Methods for producing biological substances in enzyme-deficient mutants of Aspergillus
    30.
    发明申请
    Methods for producing biological substances in enzyme-deficient mutants of Aspergillus 有权
    在曲霉菌酶缺陷突变体中生产生物物质的方法

    公开(公告)号：US20040191864A1

    公开(公告)日：2004-09-30

    申请号：US10815495

    申请日：2004-03-31

    申请人： Novozymes Biotech, Inc.

    发明人： Mariah Connelly ,  Howard Brody

    IPC分类号： C12P021/02 ,  C12P019/34 ,  C12P019/04 ,  C12N009/28 ,  C12N001/16

    CPC分类号： C07K14/37 ,  C07K14/38 ,  C07K14/40 ,  C12N9/00 ,  C12N15/52 ,  C12P1/02 ,  C12P21/02

    摘要： The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Aspergillus niger strain in a medium suitable for the production of the heterologous biological substance, wherein (i) the mutant strain comprises a first nucleotide sequence encoding the heterologous biological substance and one or more second nucleotide sequences comprising a modification of glaA and at least one of the genes selected from the group consisting of asa, amyA, amyB, prtT, and oah, and (ii) the mutant strain is deficient in the production of glucoamylase and at least one enzyme selected from the group consisting of acid stable alpha-amylase, neutral alpha-amylase A, and neutral alpha-amylase B, protease, and oxalic acid hydrolase compared to the parent Aspergillus niger strain when cultivated under identical conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Aspergillus niger strains and methods for producing such mutants.

    摘要翻译： 本发明涉及产生异源生物物质的方法，包括：（a）在适于生产异源生物物质的培养基中培养母本黑曲霉菌株的突变体，其中（i）突变株包含第一 编码异源生物物质的核苷酸序列和一个或多个第二核苷酸序列，其包含glaA的修饰和至少一种选自asa，amyA，amyB，prtT和oah的基因，以及（ii）突变株 与母本黑曲霉菌株相比，生产葡糖淀粉酶和至少一种选自酸稳定的α-淀粉酶，中性α-淀粉酶A和中性α-淀粉酶B，蛋白酶和草酸水解酶的酶不足 在相同的条件下耕种; 和（b）从培养基中回收异源生物物质。 本发明还涉及黑曲霉菌株的酶缺陷型突变体及其生产方法。