公开(公告)号：US20240229109A1

公开(公告)日：2024-07-11

申请号：US18284938

申请日：2022-03-31

Applicant: ModernaTX, Inc.

Inventor： Amy E. Rabideau ,  David Reid ,  Huijuan Li ,  Kristian Link ,  Tao Jiang ,  Eva-Maria Schneeberger

IPC: C12Q1/6825 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C12Q1/6825 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12Q2600/16

Abstract: Aspects of the disclosure relate to methods for analyzing compositions comprising RNA species with unique nucleotide sequences for identification and/or ratio determination of RNAs. The disclosure is based, in part, on methods of cleaving identifying sequences from RNAs in a composition, and detecting the abundance of each identifying sequence to quantify the abundance of corresponding RNA species. Other aspects relate to methods for producing compositions comprising more than two RNA species, such as multivalent RNA compositions. The disclosure is based, in part, on methods of determining the proper amount of input DNA for in vitro transcription (IVT) reactions that will result in RNA being transcribed in a predetermined ratio. In some aspects, the disclosure relates to pharmaceutical compositions comprising multivalent RNA compositions produced by methods described, by the disclosure.

公开(公告)号：US12030057B2

公开(公告)日：2024-07-09

申请号：US18151343

申请日：2023-01-06

Applicant: Plexium, Inc.

Inventor： Kandaswamy Vijayan ,  Andrew Boyd MacConnell ,  Joseph Franklin Rokicki

IPC: C40B30/06 ,  B01L3/00 ,  C12N15/10 ,  C12Q1/6869 ,  C12Q1/6876 ,  C40B30/04 ,  C40B50/04 ,  C40B50/14 ,  C40B50/16

CPC classification number: B01L3/50853 ,  C12N15/1034 ,  C12N15/1065 ,  C12N15/1068 ,  C12N15/1075 ,  C12Q1/6869 ,  C12Q1/6876 ,  C40B30/04 ,  C40B30/06 ,  C40B50/04 ,  C40B50/14 ,  C40B50/16 ,  B01L2300/0829 ,  C12Q2600/16 ,  C12N15/1065 ,  C12Q2563/179

Abstract: This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

公开(公告)号：US20240200154A1

公开(公告)日：2024-06-20

申请号：US18286855

申请日：2022-04-14

Applicant: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

Inventor： Jeffrey A. SORELLE

IPC: C12Q1/70 ,  C12Q1/6844 ,  C12Q1/6876

CPC classification number: C12Q1/701 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: Provided herein are compositions and methods for detecting novel SARS-COV-2 variants. Disclosed herein are nucleic acid constructs that can be used as primers in various molecular assays to identify and distinguish new variants of the SARS-COV-2 virus. Also provided are methods of using said nucleic acid constructs to identify and distinguish new variants of the SARS-COV-2 virus.

公开(公告)号：US20240200149A1

公开(公告)日：2024-06-20

申请号：US18591077

申请日：2024-02-29

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Tomomi ANDO ,  Mitsuko ISHIHARA ,  Yoshitake SANO ,  Takuya MIYAGAWA ,  Koji HASHIMOTO

IPC: C12Q1/6886 ,  C12Q1/6874

CPC classification number: C12Q1/6886 ,  C12Q1/6874 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: According to one embodiment, a method for analyzing the probability of suffering from cancer in a subject is provided. The method includes counting the number of types of RNA in a sample derived from the subject with respect to the types of RNAs in which sequence variation based on RNA editing exists in comparison with a reference sequence, and determining the probability of suffering from cancer in the subject by using the number of types of RNAs obtained as an index.

公开(公告)号：US11999774B2

公开(公告)日：2024-06-04

申请号：US17314668

申请日：2021-05-07

Applicant: Immatics Biotechnologies GmbH

Inventor： Andrea Mahr ,  Toni Weinschenk ,  Colette Song ,  Oliver Schoor ,  Jens Fritsche ,  Harpreet Singh

IPC: C07K16/00 ,  A61K35/17 ,  A61K39/00 ,  C07K14/47 ,  C07K14/635 ,  C07K14/725 ,  C07K14/74 ,  C07K16/28 ,  C12N5/0783 ,  C12Q1/6886 ,  G01N33/574 ,  A61K38/00

CPC classification number: C07K14/70539 ,  A61K35/17 ,  A61K39/0011 ,  C07K14/4748 ,  C07K14/635 ,  C07K14/7051 ,  C07K16/2833 ,  C12N5/0638 ,  C12Q1/6886 ,  G01N33/57484 ,  A61K38/00 ,  A61K39/00 ,  C07K2319/70 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The present invention relates to peptides, proteins, nucleic acids and cells for use in immunotherapeutic methods. In particular, the present invention relates to the immunotherapy of cancer. The present invention furthermore relates to tumor-associated T-cell peptide epitopes, alone or in combination with other tumor-associated peptides that can for example serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses, or to stimulate T cells ex vivo and transfer into patients. Peptides bound to molecules of the major histocompatibility complex (MHC), or peptides as such, can also be targets of antibodies, soluble T-cell receptors, and other binding molecules.

公开(公告)号：US20240175091A1

公开(公告)日：2024-05-30

申请号：US18544232

申请日：2023-12-18

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi

IPC: C12Q1/6886 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  B01F33/3031 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/686

CPC classification number: C12Q1/6886 ,  B01F23/41 ,  B01F25/4335 ,  B01F33/3011 ,  B01F33/3031 ,  B01L3/502784 ,  B01L7/52 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/686 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q2600/118 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.

公开(公告)号：US11993820B2

公开(公告)日：2024-05-28

申请号：US17234297

申请日：2021-04-19

Applicant: Quest Diagnostics Investments LLC

Inventor： Kevin Qu ,  Feras Hantash ,  Amber Donahue

IPC: C12Q1/6886 ,  C07K14/82 ,  C12N9/16 ,  C12Q1/6816 ,  C12Q1/6827

CPC classification number: C12Q1/6886 ,  C07K14/82 ,  C12N9/16 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q2600/112 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The present technology relates to methods for excluding Lynch syndrome as a possible diagnosis in patients suffering from colorectal cancers or endometrial cancers. These methods are based on detecting the methylation status of the MLH1 promoter ‘C’ region in colorectal and endometrial cancer patients using an improved and highly sensitive methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) assay.

公开(公告)号：US11993809B2

公开(公告)日：2024-05-28

申请号：US17260572

申请日：2019-07-17

Applicant: MAX-DELBRÜCK-CENTRUM FÜR MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT

Inventor： Christin Sünkel ,  Nikolaos Karaiskos ,  Nikolaus Rajewsky

IPC: C12Q1/6841 ,  C12N15/10 ,  C12Q1/6876

CPC classification number: C12Q1/6841 ,  C12N15/1065 ,  C12N15/1096 ,  C12Q1/6876 ,  C12Q2600/16

Abstract: The present invention relates to a method for analyzing a biological sample on a single cell level by compartmentalizing said sample using a grid and performing an optimized combinatorial indexing protocol within cells of the compartmentalized tissue while deducing cell-specific information regarding the cell identity and activity in the spatial context within the sample.

公开(公告)号：US20240167106A1

公开(公告)日：2024-05-23

申请号：US18509322

申请日：2023-11-15

Applicant: China Agricultural University

Inventor： Shaolin WANG ,  Yang ZUO ,  Zhangqi SHEN ,  Lei ZHOU ,  Jun HAN ,  Jianzhong SHEN

IPC: C12Q1/70 ,  C12Q1/6874 ,  C12Q1/6888

CPC classification number: C12Q1/701 ,  C12Q1/6874 ,  C12Q1/6888 ,  C12Q1/6844 ,  C12Q2600/16

Abstract: A primer group for simultaneous detection of 15 porcine pathogens through high-throughput targeted amplicon sequencing and use thereof are provided. The primer group for simultaneous detection of 15 porcine pathogens through high-throughput targeted amplicon sequencing includes: primers having nucleotide sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 302. The nucleotide sequences of pathogenic target genes are set forth in SEQ ID NO: 303 to SEQ ID NO: 344. The primer group of the present invention is used for amplicon sequencing detection analysis method for 42 target genes of 15 porcine respiratory pathogens, has the technical advantages of rapidness, high efficiency, high targeting, strong specificity, and the like, and is suitable for rapid and accurate simultaneous diagnosis of multiple pathogens of diseased pigs in pig farms.

公开(公告)号：US20240150854A1

公开(公告)日：2024-05-09

申请号：US18500048

申请日：2023-11-01

Applicant: Laboratory Corporation of America Holdings

Inventor： Thomas Jacob Urban ,  Ayla Burns Harris ,  Jonathan David Williams ,  Deborah John Boles ,  Timothy Staton ,  Nathan Harris ,  Phyllis Davis ,  Suzanne Dale

IPC: C12Q1/70 ,  C12Q1/6806

CPC classification number: C12Q1/701 ,  C12Q1/6806 ,  C12Q2600/16

Abstract: Disclosed are methods and systems to detect a class of non-variola Orthopoxviruses, and, more particularly, monkeypox virus. In certain embodiments, the method may comprise obtaining a sample from the subject; and detecting a nucleic acid sequence specific to the non-variola Orthopoxvirus. In some embodiments, the method may further isolation of the non-variola Orthopoxvirus from a solid substrate such as a tissue swab and further purification of viral nucleic acid using silica beads. The sample may in some cases be self-collected by a subject. The disclosed methods and systems, including kits for self-collection, may be used for a high throughput assay allowing for screening of hundreds of samples per day.