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    Polypeptides that bind HIV gp120 and related nucleic acids, antibodies, compositions, and methods of use
    22.
    发明申请
    Polypeptides that bind HIV gp120 and related nucleic acids, antibodies, compositions, and methods of use 失效
    结合HIV gp120和相关核酸，抗体，组合物和使用方法的多肽

    公开(公告)号：US20030068615A1

    公开(公告)日：2003-04-10

    申请号：US10084813

    申请日：2002-02-27

    申请人： Department of Health and Human Services

    发明人： Carl Saxinger

    IPC分类号： C12Q001/70 ,  C12P021/06 ,  A61K039/21 ,  C07K007/08

    CPC分类号： G01N33/531 ,  A61K2039/525 ,  G01N33/56988 ,  G01N2333/162

    摘要： The present invention provides, among other things, a polypeptide that binds with the gp120 envelope protein of HIV, in particular HIV-1, under physiological conditions, a nucleic acid that encodes such a polypeptide and can be expressed in a cell, a composition comprising such a polypeptide or nucleic acid or an antibody and a carrier therefor, a composition comprising a solid support matrix to which is attached an above-described polypeptide or an anti-antibody to a specified polypeptide sequence, a method of making an antibody to gp120, and a method of removing HIV from a bodily fluid.

    摘要翻译： 本发明尤其提供了在生理条件下与HIV，特别是HIV-1的gp120包膜蛋白结合的多肽，编码这种多肽并可在细胞中表达的核酸，包含 这样的多肽或核酸或抗体及其载体，包含与特定多肽序列连接上述多肽或抗体的固体支持基质的组合物，制备gp120抗体的方法， 以及从体液中除去HIV的方法。

    Peptides of human immunodeficiency virus type 2 (HIV-2), antibodies against peptides of HIV-2, and methods and kits for detecting HIV-2
    23.
    发明授权
    Peptides of human immunodeficiency virus type 2 (HIV-2), antibodies against peptides of HIV-2, and methods and kits for detecting HIV-2 失效
    人类免疫缺陷病毒2型（HIV-2）env多肽和使用所述肽的诊断测定

    公开(公告)号：US06514691B1

    公开(公告)日：2003-02-04

    申请号：US09191384

    申请日：1998-11-13

    申请人： Marc Alizon ,  Luc Montagnier ,  Denise Guetard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

    发明人： Marc Alizon ,  Luc Montagnier ,  Denise Guetard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

    IPC分类号： C12Q170

    CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12Q1/703 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20

    摘要： This invention is directed toward the isolation of a novel retrovirus, the human immune deficiency virus type 2 (HIV-2, previously named LAV-2), from patients with acquired immune deficiency syndrome (AIDS) originating from West Africa. This virus is related to HIV-1, the causative agent of AIDS, both by its morphology and by its tropism and in vitro cytopathic effect on CD4 (T4) positive cell lines and lymphocytes. However, preliminary hybridization experiments indicated that there are substantiated differences between the sequences of the two genomes. Furthermore, the proteins of HIV-1 and HIV-2 have different sizes and their serological cross-reactivity is restricted to the major core protein, as the envelope glycoproteins of HIV-2 are not immunoprecipitated by HIV-1 positive sera. Overlapping molecular clones were obtained and the complete nucleotide sequence of the gag and env genes was ascertained. An antigenic envelope polypeptide having the following amino acid sequence was identified: NH2-VTAIEKYLEDQARLNSWGCAFRQVC-COOH. The peptide is useful for the detection of HIV-2-specific immunological reagents.

    摘要翻译： 本发明针对来自西非的获得性免疫缺陷综合征（AIDS）患者的新型逆转录病毒，2型免疫缺陷病毒（HIV-2，以前称为LAV-2））的分离。 这种病毒与HIV-1（艾滋病的致病因子）有关，其形态以及其对CD4（T4）阳性细胞系和淋巴细胞的向性和体外细胞病变作用。 然而，初步杂交实验表明，两个基因组的序列之间存在证实的差异。 此外，HIV-1和HIV-2的蛋白质具有不同的大小，并且其血清学交叉反应性限于主要核心蛋白质，因为HIV-2的包膜糖蛋白不被HIV-1阳性血清免疫沉淀。 获得重叠的分子克隆，并确定了gag和env基因的完整核苷酸序列。 鉴定了具有以下氨基酸序列的抗原性包膜多肽：NH2-VTAIEKYLEDQA​​RLNSWGCAFRQVC-COOH。 该肽可用于检测HIV-2特异性免疫试剂。

    Nucleic acids of a human immunodeficiency virus type 2 (HIV-2)
    24.
    发明授权
    Nucleic acids of a human immunodeficiency virus type 2 (HIV-2) 失效
    人类免疫缺陷病毒2型（HIV-2）的核酸

    公开(公告)号：US06429306B1

    公开(公告)日：2002-08-06

    申请号：US08392613

    申请日：1995-02-22

    申请人： Luc Montagnier ,  Denise Guetard ,  Marc Alizon ,  François Clavel ,  Mireille Guyader ,  Pierre Sonigo ,  Françoise Brun-Vezinet

    发明人： Luc Montagnier ,  Denise Guetard ,  Marc Alizon ,  François Clavel ,  Mireille Guyader ,  Pierre Sonigo ,  Françoise Brun-Vezinet

    IPC分类号： C07H2104

    CPC分类号： C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12Q1/703 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20

    摘要： The present invention is directed toward a novel human retrovirus isolated from West African AIDS patients. This virus was originally designated lymphadenopathy associated virus (LAV) type II and subsequently renamed the human immunodeficiency virus type 2, or HIV-2. This virus is genotypically and phenotypically distinct from both human immunodeficiency virus type 1 (HIV-1) and the simian immunodeficiency virus (SIV). A recombinant &lgr; phage library was prepared by subjecting HIV-2-infected CEM genomic DNA to digestion with Sau3AI. The library was screened with an HIV-2-specific cDNA probe and molecular clones of the virus were obtained. Restriction maps and the nucleotide sequences of these clones were ascertained. These nucleic acids should prove useful, inter alia, as probes for the detection of HIV-2 in biological samples and for the expression of HIV-2 gene products.

    摘要翻译： 本发明针对从西非艾滋病患者分离的新的人类逆转录病毒。 该病毒最初被命名为II型淋巴结病相关病毒（LAV），随后更名为2型人类免疫缺陷病毒或HIV-2。 这种病毒在人类免疫缺陷病毒1型（HIV-1）和猿猴免疫缺陷病毒（SIV）的基因型和表型上不同。 通过使感染了HIV-2感染的CEM基因组DNA用Sau3AI消化来制备重组羔羊噬菌体文库。 用HIV-2特异性cDNA探针筛选文库，获得病毒的分子克隆。 确定了这些克隆的限制图和核苷酸序列。 这些核酸尤其应用于检测生物样品中的HIV-2和HIV-2基因产物的表达。

    ELECTROCHEMICAL PROBES FOR DETECTION OF MOLECULAR INTERACTIONS AND DRUG DISCOVERY
    25.
    发明申请
    ELECTROCHEMICAL PROBES FOR DETECTION OF MOLECULAR INTERACTIONS AND DRUG DISCOVERY 无效
    用于检测分子相互作用和药物发现的电化学探针

    公开(公告)号：US20020012943A1

    公开(公告)日：2002-01-31

    申请号：US09019679

    申请日：1998-02-06

    发明人： DANA M. FOWLKES ,  H. HOLDEN THORP

    IPC分类号： G01N033/53

    CPC分类号： B82Y30/00 ,  C12Q1/004 ,  G01N33/5438 ,  G01N2333/02 ,  G01N2333/162 ,  G01N2333/36 ,  G01N2333/42 ,  G01N2333/4727 ,  G01N2333/503 ,  G01N2333/70503 ,  G01N2333/70546 ,  G01N2333/7056 ,  G01N2333/71 ,  G01N2333/715 ,  G01N2333/745 ,  G01N2333/78 ,  G01N2333/8107 ,  G01N2333/9726

    摘要： This invention relates to methods and apparati for performing electrochemical analyses. The invention provides an electrochemical apparatus for performing potentio metric analyses for detecting specific binding between a first member of a biological binding pair immobilized on an electrode and a second member of a biological binding pair that is electrochemically labeled, in the presence of an electrochemical mediator. Methods for using the apparatus of the invention for performing binding and competition binding assays are provided. The invention also provides methods for performing high throughput screening assays for detecting inhibition of specific binding between the members of the biological binding pair for use in drug development, biochemical analysis and protein purification assays.

    摘要翻译： 本发明涉及进行电化学分析的方法和装置。 本发明提供了一种电化学装置，用于在电化学介体的存在下进行电位计量分析，以检测固定在电极上的生物结合对的第一个成员与电化学标记的生物结合对的第二个成员之间的特异性结合。 提供了使用本发明的装置进行结合和竞争结合测定的方法。 本发明还提供了用于进行高通量筛选测定以检测用于药物开发，生物化学分析和蛋白质纯化测定中的生物结合对成员之间特异性结合的抑制的方法。

    Immunodissociation for improving the immunochemical determination of an analyte
    28.
    发明授权
    Immunodissociation for improving the immunochemical determination of an analyte 失效
    用于改善分析物的免疫化学测定的免疫解离

    公开(公告)号：US5989806A

    公开(公告)日：1999-11-23

    申请号：US992007

    申请日：1997-12-17

    申请人： Stefan Brust

    发明人： Stefan Brust

    IPC分类号： G01N33/543 ,  G01N33/569

    CPC分类号： G01N33/54306 ,  G01N33/56988 ,  G01N2333/162 ,  Y10S435/962 ,  Y10S435/974

    摘要： The present invention relates to a process for the immunochemical determination of one or more analytes in a sample using an immobilized specific receptor R1, which exhibits interactive bioaffinity with the analyte, and a specific receptor R2, which likewise exhibits interactive bioaffinity with the analyte and which as a rule is labeled. In the novel process, a receptor R3, which possesses one or more than one specific binding site for the analyte, is added and the resulting immune complexes are entirely or partially dissociated, after which they are reassociated and subsequently detected. According to a further embodiment, a receptor R4 is employed in addition to the receptor R3, which receptor R4 possesses an affinity towards R3 and is immobilized on the solid phase, with the resulting immune complexes being entirely or partially dissociated, after which they are reassociated and subsequently detected.

    摘要翻译： 本发明涉及用样品中一种或多种分析物进行免疫化学测定的方法，所述固定化的特异性受体R1与被分析物呈现出相互作用的生物亲和性，并且特异性受体R2与分析物同样具有互动的生物亲和性， 通常被标记。 在该新方法中，加入具有一个或多于一个分析物特异性结合位点的受体R3，并且所得免疫复合物完全或部分解离，之后它们被重新结合并随后检测。 根据另一个实施方案，除了受体R 3之外还使用受体R4，该受体R 4对R 3具有亲和力并固定在固相上，所得到的免疫复合物完全或部分解离，之后它们重新结合 并随后检测。