公开(公告)号：US5948895A

公开(公告)日：1999-09-07

申请号：US893161

申请日：1997-07-15

申请人： Koji Sugiyama ,  Akane Ohnaka

发明人： Koji Sugiyama ,  Akane Ohnaka

IPC分类号： A61K38/00 ,  C07K1/107 ,  C07K9/00 ,  C07K14/76 ,  A61K38/14

CPC分类号： C07K1/1077 ,  C07K9/00

摘要： A process which makes possible to produce a glycosylated amino compound (e.g. protein) easily in short period without denaturation of protein. The sugar (e.g. glucose) and protein (e.g. serum) are mixed, and the mixture is dried under reduced pressure, so that sugar and protein quickly react with each other to produce the glycosylated protein. The drying under reduced pressure is preferably lyophilization which is conducted under the following conditions: the temperature is about -20.degree. C., the pressure is about 2 mmHg, and the treatment time is 12 hours. Unreacted glucose is then removed by the dialysis process, thus providing the required glycosylated protein sterilization need not be carried out. This process makes it possible to produce a glycosylated protein in about one day as compared to 7 days by the conventional process, so that the denaturation of protein is less likely to occur.

摘要翻译： 可以在短时间内容易地生成糖基化氨基化合物（例如蛋白质）而不使蛋白质变性的方法。 将糖（例如葡萄糖）和蛋白质（例如血清）混合，并将混合物在减压下干燥，使得糖和蛋白质彼此快速反应以产生糖基化蛋白质。 减压干燥优选为在以下条件下进行的冷冻干燥：温度为约-20℃，压力为约2mmHg，处理时间为12小时。 然后通过透析过程除去未反应的葡萄糖，从而提供所需的糖基化蛋白质灭菌不需要进行。 与常规方法相比，该方法可以在约1天内产生糖基化蛋白质，从而不太可能发生蛋白质的变性。

公开(公告)号：US5846359A

公开(公告)日：1998-12-08

申请号：US598528

申请日：1996-02-08

申请人： Minoru Ota ,  Yoshinori Takahashi ,  Yoshihiko Higuchi ,  Takashi Tsujii

发明人： Minoru Ota ,  Yoshinori Takahashi ,  Yoshihiko Higuchi ,  Takashi Tsujii

IPC分类号： B29C65/00 ,  B29C65/02 ,  B29C65/06 ,  B29C65/08 ,  G01N33/52 ,  B32B31/16

CPC分类号： G01N33/525 ,  B29C65/08 ,  B29C65/082 ,  B29C66/472 ,  B29C66/7392 ,  B29C66/7487 ,  B29C65/0618 ,  B29C66/21 ,  B29C66/303 ,  B29C66/30326 ,  B29C66/71 ,  B29C66/727 ,  B29C66/729 ,  B29C66/73921 ,  B29K2313/00 ,  B29K2705/00 ,  B29K2709/02 ,  B29K2709/08 ,  B29K2711/00 ,  B29K2711/12 ,  B29K2711/14

摘要： A method for fixing a reagent layer directly onto a supporting base plate in the preparation of a dry analysis kit for determining a specific component in a liquid specimen wherein at least one of the reagent layer and the base plate is a thermoplastic resin, and which comprises the steps of: placing the reagent layer in contact with the base plate and externally applying ultrasonic vibration and pressure to the two layers to generate frictional heat thereby to melt the surface of the thermoplastic resin; applying pressure to make the molten surface of thermoplastic resin bite the non-thermoplastic material or to integrate the surfaces of thermoplastic layer and base plate; and removing the ultrasonic vibration and pressure.

公开(公告)号：US5844239A

公开(公告)日：1998-12-01

申请号：US858567

申请日：1997-05-19

申请人： Eiichi Kimura

发明人： Eiichi Kimura

IPC分类号： G01N21/35 ,  A61B5/00 ,  A61B10/00 ,  G01N21/47 ,  G01N21/49 ,  G02B6/04

CPC分类号： A61B5/1455 ,  A61B5/14532 ,  G01N21/474 ,  G02B6/04 ,  G01N2021/4747 ,  G01N21/49

摘要： Light of a specific wavelength is introduced into respective optical fiber members forming a light projecting optical fiber member group (14a) through a condensing lens (26). A second end of an optical fiber light guide path (10) whose first end is brought into close contact with a target (20) is branched into three optical fiber member groups including a light projecting optical fiber member group (14a) and first and second photoreceiving optical fiber member groups (16a, 18a), and each of the optical fiber member groups (14a, 16a, 18a) is formed by bundling optical fiber members forming respective unit bundles respectively. Each unit bundle includes at least one light projecting optical fiber member which is arranged at the center, a first photoreceiving optical fiber member group arranged around the light projecting optical fiber member substantially on the circumference of a first circle concentric with the optical fiber member, and a second photoreceiving optical fiber member group arranged substantially on the circumference of a second circle which is concentric with the at least one optical fiber member and larger in radius than the first circle.

摘要翻译： 将特定波长的光通过聚光透镜（26）引入形成光投射用光纤部件组（14a）的各个光纤部件。 其第一端与靶（20）紧密接触的光纤导光路径（10）的第二端被分支成包括光投射光纤部件组（14a）的第一和第二光纤部件组 光接收光纤部件组（16a，18a），并且通过分别形成各个单元束的光纤部件来形成光纤部件组（14a，16a，18a）。 每个单元束包括布置在中心的至少一个光投射光纤部件，基本上在与光纤部件同心的第一圆周的圆周上布置有光投射光纤部件的第一光接收光纤部件组，以及 第二光接收光纤部件组，其基本上布置在与所述至少一个光纤部件同心并且比所述第一圆的半径大的第二圆的圆周上。

公开(公告)号：US5824527A

公开(公告)日：1998-10-20

申请号：US630175

申请日：1996-04-11

申请人： Nobuo Kato ,  Yasuyoshi Sakai ,  Yoshiki Tani ,  Masayuki Yagi ,  Fumiyo Funatsu

发明人： Nobuo Kato ,  Yasuyoshi Sakai ,  Yoshiki Tani ,  Masayuki Yagi ,  Fumiyo Funatsu

IPC分类号： C12N9/06 ,  C12N9/02 ,  C12Q1/26

CPC分类号： C12N9/0022 ,  C12R1/80

摘要： A novel fructosyl amino acid oxidase derived from genus Penicillium, which is active on both of fructosyl lysine and fructosyl valine, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme is provided.

摘要翻译： 提供了一种来源于青霉属的新型果糖基氨基酸氧化酶，其在果糖基赖氨酸和果糖基缬氨酸两者上均有活性，提供了生产酶的方法，使用该酶的阿马多利化合物的测定法，含有该酶的试剂或试剂盒 。

公开(公告)号：US5615009A

公开(公告)日：1997-03-25

申请号：US630974

申请日：1996-04-12

申请人： Takeshi Sakura ,  Yutaka Yamasaki ,  Hiroko Kubo ,  Kexin Xu ,  Motonobu Shiomi

发明人： Takeshi Sakura ,  Yutaka Yamasaki ,  Hiroko Kubo ,  Kexin Xu ,  Motonobu Shiomi

IPC分类号： G01J3/28 ,  G01N21/27 ,  G01J3/42

CPC分类号： G01N21/274

摘要： A method of quantitative analysis of a specific component of an object to be measured comprises steps of measuring an energy spectrum of light transmitted through or reflected from the object, dividing the energy spectrum into a plurality of wavelength domains, thereby obtaining a plurality of partial energy spectra, normalizing the plurality of partial energy spectra within each wavelength domain using an energy measured at a predetermined wavelength contained in each wavelength domain, and performing the quantative analysis by multivariate analysis using the plurality of partial energy spectra having been normalized.

摘要翻译： 对被测量物体的特定成分进行定量分析的方法包括以下步骤：测量透过物体或通过物体反射的光的能谱，将能谱分成多个波长域，从而获得多个部分能量 光谱，使用在每个波长域中包含的预定波长测量的能量对每个波长域内的多个部分能谱进行标准化，以及使用已经被归一化的多个部分能谱通过多变量分析进行定量分析。

公开(公告)号：US5602647A

公开(公告)日：1997-02-11

申请号：US562785

申请日：1995-11-27

申请人： Kexin Xu ,  Yutaka Yamasaki ,  Harumi Uenoyama ,  Takeshi Sakura

发明人： Kexin Xu ,  Yutaka Yamasaki ,  Harumi Uenoyama ,  Takeshi Sakura

IPC分类号： G01N21/27 ,  G01J3/42 ,  G01N21/03 ,  G01N21/31 ,  G01N21/39 ,  G01N21/59 ,  G01N21/00

CPC分类号： G01N21/0303 ,  G01N21/31 ,  G01J3/42 ,  G01N2021/3129 ,  G01N21/39 ,  G01N21/59 ,  G01N2201/066 ,  G01N2201/0668

摘要： An apparatus and method for optically measuring concentrations of components allow enhancement in measurement accuracy of concentration. The apparatus includes a cell, a light irradiator, a photodetector, and an arithmetic unit. The cell presents different optical path lengths at different locations and is to contain a sample therein. The light irradiator, which includes a variable-wavelength laser generator and a measuring system composed of convex lenses, outputs a collimated, enlarged laser beam, and makes the laser beam incident upon the cell. The photodetector comprises a multiplicity of photodetectors arranged in parallel to the surface of the cell, so that it can detect intensity of rays of transmitted light that have traveled over different optical path lengths at positions of an equal distance from the cell. The arithmetic unit, receiving a signal from the individual photodetectors, calculates concentrations of components in the sample based on optimum optical path lengths for different wavelengths and values of transmitted light at positions of the optimum optical path lengths, and further outputs calculation results.

摘要翻译： 用于光学测量组分浓度的装置和方法允许增强浓度的测量精度。 该装置包括单元，光照射器，光电检测器和运算单元。 细胞在不同位置呈现不同的光程长度，并且其中包含样品。 包括可变波长激光发生器和由凸透镜组成的测量系统的光照射器输出准直的，放大的激光束，并使激光束入射到电池上。 光电检测器包括与电池表面平行设置的多个光电检测器，使得其可以检测在距离电池相等距离的位置处已经在不同光程长度上行进的透射光的光强。 运算单元接收来自各个光电检测器的信号，根据最佳光程长度的不同波长的最佳光路长度和最佳光路长度位置处的透射光值，计算样品中的成分浓度，并进一步输出计算结果。

公开(公告)号：US5496453A

公开(公告)日：1996-03-05

申请号：US321604

申请日：1994-10-12

申请人： Harumi Uenoyama ,  Hisashi Okuda

发明人： Harumi Uenoyama ,  Hisashi Okuda

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/416 ,  G01N27/26

CPC分类号： C12Q1/004 ,  Y10S435/817

摘要： A biosensor which electrochemically detects a material which relates to a reaction of a specific compound in a liquid sample with a biologically active substance or its related substance, in which the biologically active material or an optionally used mediator is placed at a part which is remote from a position of an electrode which acts as electrochemical detector means, and optionally the biological active material or the mediator is covered with a polymer layer.

公开(公告)号：US5304467A

公开(公告)日：1994-04-19

申请号：US809253

申请日：1991-12-18

申请人： Hisashi Sakamoto ,  Shigeki Yamada ,  Hiroshi Taniguchi

发明人： Hisashi Sakamoto ,  Shigeki Yamada ,  Hiroshi Taniguchi

IPC分类号： G01N33/52 ,  G01N31/22 ,  C12Q1/28 ,  C12Q1/54 ,  C12Q1/62

CPC分类号： G01N33/525 ,  Y10S435/805

摘要： A device for colorimetric assay of at least one component in a liquid sample, which device comprises a support, a reagent layer formed on a part of one surface of the support and a sample-receiving layer which covers at least a part of the reagent layer and at least a part of the support surface, with which the timing of application of a sample on the device is automatically detected with accuracy.

摘要翻译： 一种用于液体样品中至少一种组分的比色测定装置，该装置包括载体，形成在载体的一个表面的一部分上的试剂层和覆盖试剂层的至少一部分的样品接收层 以及至少一部分支撑表面，其中准确地自动检测样品在装置上的施加时间。

公开(公告)号：US5266179A

公开(公告)日：1993-11-30

申请号：US733130

申请日：1991-07-19

申请人： Shiro Nankai ,  Mariko Kawaguri ,  Toshihiko Yoshioka ,  Haruhiro Tsutsumi ,  Kyozo Terao ,  Naoki Tanimoto ,  Masahiro Yoshioka ,  Hiroshi Hyodo ,  Takatoshi Uchigaki

发明人： Shiro Nankai ,  Mariko Kawaguri ,  Toshihiko Yoshioka ,  Haruhiro Tsutsumi ,  Kyozo Terao ,  Naoki Tanimoto ,  Masahiro Yoshioka ,  Hiroshi Hyodo ,  Takatoshi Uchigaki

IPC分类号： G01N27/26 ,  G01N27/28 ,  G01N27/416 ,  G01N27/49

CPC分类号： G01N33/48771 ,  G01N2035/009

摘要： A sensor 13 is inserted into a connector 14. A constant voltage required to obtain a response current is applied across the connector 14 by a voltage applying source 15 at timings required. A response current of the sensor 13 inserted into the connector 14 is converted into a voltage by a current-to-voltage converter 16, and the amount thereof is determined by a microcomputer, the analysis results being displayed onto a display unit.

公开(公告)号：US07393502B2

公开(公告)日：2008-07-01

申请号：US10208816

申请日：2002-08-01

申请人： Akio Okubo ,  Atsuko Katayama ,  Yoshiyuki Tanaka ,  Yoshihiko Higuchi ,  Masufumi Koike

发明人： Akio Okubo ,  Atsuko Katayama ,  Yoshiyuki Tanaka ,  Yoshihiko Higuchi ,  Masufumi Koike

IPC分类号： G01N21/00 ,  G01N35/02 ,  G01N15/06

CPC分类号： B01L3/5027 ,  B01L2200/0621 ,  B01L2300/0681 ,  B01L2300/0822 ,  B01L2300/0825 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0688 ,  B01L2400/0694 ,  Y10T436/114165 ,  Y10T436/114998

摘要： A test device 1 for analyzing a specific component in a test solution with a reagent by allowing the solution introduced via a feed opening 4 to react with the reagent maintained in a predetermined position in a capillary tube 3 having the opening 4 and an air outlet 5. The tube is provided with two hydrophilic regions 31, 33 and a hydrophobic region 32. The region 31 transfers the solution from the opening 4 to the reagent. The region 33 is delimited to a predetermined area maintaining the reagent. The region 32 separates the region 31 from the region 33. The reagent and the solution are applied in predetermined amounts to the region 33. A measuring device need not previously measure the solution. The device is useful as an analytical device for rapid and easy analysis, and can be produced in a less number of steps because the reagent can be fixed by merely applying it onto a predetermined position.

摘要翻译： 一种测试装置1，用于通过使经由进料口4引入的溶液与保持在具有开口4的毛细管3中的预定位置的试剂和空气出口5反应，用试剂分析测试溶液中的特定成分 。 该管设置有两个亲水区域31,33和疏水区域32。 区域31将溶液从开口4转移到试剂。 区域33被界定为维持试剂的预定区域。 区域32将区域31与区域33分离。 将试剂和溶液以预定量施加到区域33。 测量装置不需要事先测量解决方案。 该装置可用作用于快速和容易分析的分析装置，并且可以以较少数量的步骤生产，因为试剂可以通过仅将其施加到预定位置来固定。