公开(公告)号：US20170361298A1

公开(公告)日：2017-12-21

申请号：US15697904

申请日：2017-09-07

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: B01J19/00 ,  G05D23/20

CPC classification number: B01J19/0046 ,  B01J2219/00495 ,  B01J2219/00698 ,  G05D23/1919 ,  G05D23/2034 ,  G05D23/2037

Abstract: A system for regulating a temperature of a measurement array is disclosed. The system includes a measurement array including a plurality of sensors, wherein the plurality of sensors are integrated onto an integrated circuit die. The system includes a thermal sensor integrated onto the integrated circuit die, wherein the thermal sensor senses a temperature associated with the plurality of sensors. The system further includes a heat pump coupled to the integrated circuit die, wherein the heat pump is controlled by a feedback control circuit including the thermal sensor.

公开(公告)号：US20170283867A1

公开(公告)日：2017-10-05

申请号：US15085713

申请日：2016-03-30

Applicant: Genia Technologies, Inc.

Inventor： Ashraf Wahba ,  William Nielsen ,  J. William Maney, JR. ,  Kevin Aliado ,  Kyle Umeda ,  Roger J.A. Chen ,  Jing Luo

IPC: C12Q1/68

Abstract: A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A salt buffer solution is flowed over the array of cells in the nanopore based sequencing chip to substantially fill the wells in the cells with the salt buffer solution. A lipid and solvent mixture is flowed over the array of cells to deposit the lipid and solvent mixture over at least some of the wells in the cells. A first portion of the cells, each having a lipid bilayer over its well, is detected. A second portion of the cells, each having a lipid membrane but not a lipid bilayer over its well, is detected. An electrical lipid-thinning stimulus is selectively applied to the second portion of the cells but not to the first portion of the cells.

公开(公告)号：US20170283866A1

公开(公告)日：2017-10-05

申请号：US15085700

申请日：2016-03-30

Applicant: Genia Technologies, Inc.

Inventor： Ashraf Wahba ,  William Nielsen ,  Jing Luo ,  Kevin Aliado ,  Kyle Umeda ,  Roger J.A. Chen

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  G01N33/48721 ,  C12Q2565/607

Abstract: A method of detecting a lipid bilayer formed in a cell of a nanopore based sequencing chip is disclosed. An integrating capacitor is coupled with a lipid membrane, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to a change in the AC voltage is determined. Whether the lipid membrane comprises a lipid bilayer is detected based on the determined change in the sampled voltage across the integrating capacitor in response to the change in the AC voltage.

公开(公告)号：US20170267983A1

公开(公告)日：2017-09-21

申请号：US15444020

申请日：2017-02-27

Applicant: Genia Technologies, Inc.

Inventor： Aruna Ayer ,  Arkadiusz Bibillo ,  Preethi Sarvabhowman ,  Dhruti Vasudev Dalal ,  Ilya Lederman ,  Eileen Thai

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C12Y207/07007

Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.

公开(公告)号：US09678055B2

公开(公告)日：2017-06-13

申请号：US14150322

申请日：2014-01-08

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Randy Davis

IPC: G01N33/487 ,  G01N15/12 ,  B81B1/00 ,  B82Y5/00

CPC classification number: G01N33/48721 ,  B81B1/00 ,  B82Y5/00 ,  G01N15/12

Abstract: A method of forming a nanopore in a lipid bilayer is disclosed. A nanopore forming solution is deposited over a lipid bilayer. The nanopore forming solution has a concentration level and a corresponding activity level of pore molecules such that nanopores are substantially not formed un-stimulated in the lipid bilayer. Formation of a nanopore in the lipid bilayer is initiated by applying an agitation stimulus level to the lipid bilayer. In some embodiments, the concentration level and the corresponding activity level of pore molecules are at levels such that less than 30 percent of a plurality of available lipid bilayers have nanopores formed un-stimulated therein.

公开(公告)号：US20170103952A1

公开(公告)日：2017-04-13

申请号：US15240872

申请日：2016-08-18

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: H01L23/552 ,  G01N33/487 ,  G01N27/447 ,  H01L23/522 ,  H01L23/58

CPC classification number: H01L23/552 ,  B82Y15/00 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/48721 ,  H01L21/76877 ,  H01L23/5225 ,  H01L23/585 ,  H01L28/60 ,  H01L2924/0002 ,  H01L2924/00

Abstract: A device having an integrated noise shield is disclosed. The device includes a plurality of vertical shielding structures substantially surrounding a semiconductor device. The device further includes an opening above the semiconductor device substantially filled with a conductive fluid, wherein the plurality of vertical shielding structures and the conductive fluid shield the semiconductor device from ambient radiation. In some embodiments, the device further includes a conductive bottom shield below the semiconductor device shielding the semiconductor device from ambient radiation. In some embodiments, the opening is configured to allow a biological sample to be introduced into the semiconductor device. In some embodiments, the vertical shielding structures comprise a plurality of vias, wherein each of the plurality of vias connects more than one conductive layers together. In some embodiments, the device comprises a nanopore device, and wherein the nanopore device comprises a single cell of a nanopore array.

公开(公告)号：US09617595B2

公开(公告)日：2017-04-11

申请号：US14334523

申请日：2014-07-17

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Randy Davis

IPC: C12Q1/68 ,  B82Y5/00 ,  G01N33/487

CPC classification number: C12Q1/6876 ,  B82Y5/00 ,  G01N33/48721

Abstract: Techniques for assembling a lipid bilayer on a substantially planar solid surface are described herein. In one example, a lipid material such as a lipid suspension is deposited on a substantially planar solid surface, a bubble filled with fast diffusing gas molecules is formed on the solid surface, and the gas molecules are allowed to diffuse out of the bubble to form a lipid bilayer on the solid surface.

公开(公告)号：US09617593B2

公开(公告)日：2017-04-11

申请号：US14471051

申请日：2014-08-28

Applicant: Genia Technologies, Inc.

Inventor： Randall W. Davis ,  Roger J. A. Chen

IPC: G01N27/447 ,  C12Q1/68 ,  G01N33/487 ,  B82Y15/00 ,  B82Y10/00

CPC classification number: C12Q1/6869 ,  B82Y10/00 ,  B82Y15/00 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N27/447 ,  G01N33/48721 ,  Y10S977/924 ,  C12Q2525/119 ,  C12Q2525/121 ,  C12Q2525/204 ,  C12Q2527/101 ,  C12Q2563/149 ,  C12Q2565/631

Abstract: The present invention relates to a method of using nanopores to obtain sequence information of sample DNAs in ss test DNAs. The method comprises using speed bumps to stall the ss test DNAs in the nanopores at random positions of the ss test DNAs to obtain sequence information of each and every nucleotides of the sample DNAs, and to construct the whole sequences of the sample DNAs. The present invention also relates to identification and/or isolation of test DNAs having desired sequence(s) using nanopore detectors facilitated by speed bump.

公开(公告)号：US20170089857A1

公开(公告)日：2017-03-30

申请号：US14864395

申请日：2015-09-24

Applicant: Genia Technologies, Inc.

Inventor： Bill Maney ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N27/26 ,  B82Y35/00

CPC classification number: G01N27/26 ,  B82Y35/00 ,  G01N33/48721

Abstract: A nanopore measurement circuit is disclosed. The nanopore measurement circuit includes a nanopore electrode, a first analog memory and a second analog memory. The nanopore measurement circuit also includes a switch network that selectively connects the nanopore electrode to at least one of the first analog and the second analog memory.

公开(公告)号：US09605309B2

公开(公告)日：2017-03-28

申请号：US14073445

申请日：2013-11-06

Applicant: Genia Technologies, Inc.

Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  G01N27/44717 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/113 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2565/631

Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.