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    ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS
    37.
    发明申请
    ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS 审中-公开
    用于植物基因工程的土地垫

    公开(公告)号：US20150040267A1

    公开(公告)日：2015-02-05

    申请号：US14458086

    申请日：2014-08-12

    Applicant: Dow AgroSciences LLC

    Inventor： William Michael Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Richard Corbin ,  Rebecca Ruth Miles ,  Steven R. Webb

    IPC: C12N15/82

    CPC classification number: C12N15/8201 ,  C12N15/8213 ,  C12N15/8241

    Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

    Abstract translation: 一种用于生产转基因植物的方法包括提供核酸分子，其包含与植物细胞的基因组DNA缺乏序列同源性的至少两个核酸序列区域和至少两个锌指核酸酶核酸酶识别位点，其中所述至少两个区域 与在至少两个锌指核酸酶识别位点相邻的植物细胞的基因组DNA缺乏序列同源性的核酸序列。 转化具有稳定整合到植物细胞基因组中的核酸分子的植物细胞或组织。 植物从植物细胞再生。 通过该方法产生转基因植物。 种子由转基因植物产生。

    DNA DETECTION METHODS FOR SITE SPECIFIC NUCLEASE ACTIVITY
    39.
    发明申请
    DNA DETECTION METHODS FOR SITE SPECIFIC NUCLEASE ACTIVITY 有权
    用于现场特异性核素活性的DNA检测方法

    公开(公告)号：US20140298547A1

    公开(公告)日：2014-10-02

    申请号：US14105223

    申请日：2013-12-13

    Applicant: Dow AgroSciences LLC

    Inventor： Lakshmi Sastry-Dent ,  Matthew Simpson ,  Zehui Cao ,  Wei Chen ,  Ning Zhou ,  Steven R. Webb

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  A01G24/00 ,  C12N5/00 ,  C12N15/1082 ,  C12N15/66 ,  C12N15/70 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C40B40/08

    Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

    Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座的完整性或破坏的高通量过程，并且任选地用于检测目标基因组基因座上的供体DNA多核苷酸插入。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。

    ENGINEERED TRANSGENE INTEGRATION PLATFORM (ETIP) FOR GENE TARGETING AND TRAIT STACKING
    40.
    发明申请
    ENGINEERED TRANSGENE INTEGRATION PLATFORM (ETIP) FOR GENE TARGETING AND TRAIT STACKING 审中-公开
    用于基因定位和路由堆栈的工程变换集成平台（ETIP）

    公开(公告)号：US20140090113A1

    公开(公告)日：2014-03-27

    申请号：US14020575

    申请日：2013-09-06

    Applicant: Dow AgroSciences LLC

    Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Mike Ainley ,  Matthew J. Henry ,  John Mason ,  Sandeep Kumar ,  Stephen Novak

    IPC: C12N15/82

    Abstract: An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5′ and 3′ ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

    Abstract translation: 描述了可以随机或在植物基因组中的目标位置插入工程化转基因整合平台（ETIP），以便于在ETIP基因组位置上完全靶向（5'和3'末端）的GOI的快速选择和检测 。 主题披露中的一个要素是在ETIP内引入特定的双链断裂。 在一些实施方案中，使用锌指核酸酶结合位点描述ETIP，但可以利用其它靶向技术，例如大范围核酸酶，CRISPR，TAL或亮氨酸拉链。 还描述了转基因植物的组合物和生产方法，其中供体或有效载体DNA表达已经稳定整合到植物细胞中的ETIP中的外源核酸序列（例如蛋白质或RNA）的一种或多种产物。 在实施方案中，ETIP有助于从构想到开发阶段测试基因候选物和植物表达载体。

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