公开(公告)号：US20200325517A1

公开(公告)日：2020-10-15

申请号：US16806812

申请日：2020-03-02

Applicant: Evolva SA

Inventor： Jens Houghton-Larsen ,  Katarzyna Krzystanek ,  Angelika Semmler ,  Iver Klavs Riishede Hansen ,  Soren Damkiaer ,  Yaoquan Liu ,  Jorgen Hansen ,  Sathish Kumar ,  Muthuswamy Panchapagesa Murali ,  Nina Nicoline Rasmussen

IPC: C12P33/00 ,  C12P19/56 ,  C12P19/18 ,  C12N9/14 ,  C12N9/10 ,  C12N9/06 ,  C12N9/90 ,  C12N9/02 ,  A23L27/30 ,  C07H1/06 ,  C12N15/00

Abstract: Methods for recombinant and enzymatic production of mogroside compounds and compositions containing mogroside compounds are provided by this invention.

公开(公告)号：US20190106722A1

公开(公告)日：2019-04-11

申请号：US16095564

申请日：2017-04-12

Applicant: EVOLVA SA

Inventor： Laura Occhipinti ,  Yiming Chang ,  Jorgen Hansen

IPC: C12P19/60 ,  C12N9/10 ,  C12N9/02

Abstract: The invention relates to methods for producing melanin and melanin precursors, derivatives, and intermediates. In particular, recombinant microorganisms are disclosed that express tyrosinases to produce 5,6-DHI and express UGT polypeptides capable of either in vivo or in vitro glycosylation of melanin precursors, derivatives, and intermediates. Glycosylated 5,6-DHI is produced both in vivo and in vitro.

公开(公告)号：US10011859B2

公开(公告)日：2018-07-03

申请号：US14504109

申请日：2014-10-01

Applicant: Evolva SA

Inventor： Yaoquan Liu ,  Jorgen Hansen ,  Jens Houghton-Larsen ,  Muthuswamy Panchapagesa Murali ,  Sathish Kumar ,  Nina Nicoline Rasmussen

IPC: C12P33/20 ,  C12P19/18 ,  C12P19/56 ,  C12N1/00 ,  C12N9/10 ,  C12P33/08 ,  C12N9/02 ,  C12N9/14 ,  C12N9/90 ,  C12P33/00 ,  C12P33/12 ,  C12N15/80

CPC classification number: C12P33/20 ,  C12N9/0071 ,  C12N9/0073 ,  C12N9/1048 ,  C12N9/14 ,  C12N9/90 ,  C12N15/80 ,  C12P19/18 ,  C12P19/56 ,  C12P33/00 ,  C12P33/08 ,  C12P33/12 ,  C12Y114/00 ,  C12Y114/13132 ,  C12Y303/00 ,  C12Y504/99033

Abstract: The invention relates to methods for producing mogrosides with the aid of enzymes. In particular the invention proposes various biosynthetic pathways useful for mogroside production and enzymes useful for mogroside production are provided. Furthermore, the invention provides recombinant hosts useful in performing the methods of the invention.

公开(公告)号：US20150361476A1

公开(公告)日：2015-12-17

申请号：US14764898

申请日：2014-02-11

Applicant: EVOLVA SA

Inventor： Ernesto Simon ,  Iben Nordmark Anderson ,  Michael Dalgaard Mikkelsen ,  Jorgen Hansen ,  Veronique Douchin

IPC: C12P19/56 ,  C07K14/395 ,  C12N9/88 ,  C12N9/10 ,  C12N9/02

CPC classification number: C12P19/56 ,  C07K14/395 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/1062 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12P15/00 ,  C12Y106/02004 ,  C12Y114/13078 ,  C12Y204/00 ,  C12Y204/01013 ,  C12Y204/01035 ,  C12Y205/01029 ,  C12Y402/03 ,  C12Y402/03019 ,  C12Y505/01013

Abstract: Recombinant microorganisms are disclosed that produce steviol glycosides and have altered expression of one or more endogenous transporter or transcription factor genes, or that overexpress one or more heterologous transporters, leading to increased excretion of steviol glycosides of interest.

Abstract translation: 公开了重组微生物，其产生甜菊糖苷并且改变一种或多种内源性转运蛋白或转录因子基因的表达，或者过表达一种或多种异源转运蛋白，导致感兴趣的甜菊糖苷的排泄增加。

公开(公告)号：US20150342234A1

公开(公告)日：2015-12-03

申请号：US14648747

申请日：2013-12-04

Applicant: Evolva SA

Inventor： Paula Hicks ,  Michael Mikkelsen ,  Jens Houghton-Larsen ,  Jorgen Hansen ,  Hans Peter Smits ,  Jens Mueller

IPC: A23L1/236 ,  C12P19/56

CPC classification number: C12P19/56 ,  A23L27/33 ,  A23L27/36 ,  A23V2002/00

Abstract: Materials and methods for producing particular steviol glycosides, and high-purity compositions of particular steviol glycosides with improved sensory profilesare provided herein. The steviol glycosides can be, for example, rebaudioside D, rebaudioside A, and rebaudioside B, and can be produced by recombinant microorganisms.

Abstract translation: 本文提供了用于生产特定甜菊糖苷的材料和方法，以及具有改善的感官特性的特定甜菊糖苷的高纯度组合物。 甜菊糖苷可以是例如新蛇菊苷D，莱鲍迪甙A和莱鲍迪甙B，并且可以通过重组微生物产生。

公开(公告)号：US20150064743A1

公开(公告)日：2015-03-05

申请号：US14504109

申请日：2014-10-01

Applicant: Evolva SA

Inventor： Yaoquan Liu ,  Jorgen Hansen ,  Jens Houghton-Larsen ,  Muthuswamy Panchapagesa Murali ,  Sathish Kumar ,  Nina Nicoline Rasmussen

IPC: C12P33/20 ,  C12P33/00 ,  C12N9/02 ,  C12P33/12

CPC classification number: C12P33/20 ,  C12N9/0071 ,  C12N9/0073 ,  C12N9/1048 ,  C12N9/14 ,  C12N9/90 ,  C12N15/80 ,  C12P19/18 ,  C12P19/56 ,  C12P33/00 ,  C12P33/08 ,  C12P33/12 ,  C12Y114/00 ,  C12Y114/13132 ,  C12Y303/00 ,  C12Y504/99033

Abstract: The invention relates to methods for producing mogrosides with the aid of enzymes. In particular the invention proposes various biosynthetic pathways useful for mogroside production and enzymes useful for mogroside production are provided. Furthermore, the invention provides recombinant hosts useful in performing the methods of the invention.

Abstract translation: 本发明涉及借助于酶的方法来生产芒果苷。 特别地，本发明提出了可用于罗汉果苷生产的各种生物合成途径，并提供了可用于罗汉果苷生产的酶。 此外，本发明提供可用于实施本发明方法的重组宿主。