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    ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL
    32.
    发明申请
    ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL 审中-公开

    公开(公告)号：US20170275654A1

    公开(公告)日：2017-09-28

    申请号：US15616841

    申请日：2017-06-07

    Applicant: Genomatica, Inc.

    Inventor： Anthony P. BURGARD ,  Mark J. BURK ,  Robin E. OSTERHOUT ,  Priti PHARKYA

    IPC: C12P7/18 ,  C12N9/88 ,  C12N9/04 ,  C12N9/02 ,  C07C29/80 ,  C12N15/52 ,  C12N9/10

    CPC classification number: C12P7/18 ,  C07C29/80 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01 ,  C12Y101/01157 ,  C12Y102/01 ,  C12Y203/01 ,  C12Y206/01 ,  C12Y401/01 ,  C12Y402/01 ,  C12Y403/01

    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2-one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

    MICROORGANISMS AND METHODS FOR IMPROVING PRODUCT YIELDS ON METHANOL USING ACETYL-COA SYNTHESIS
    34.
    发明申请
    MICROORGANISMS AND METHODS FOR IMPROVING PRODUCT YIELDS ON METHANOL USING ACETYL-COA SYNTHESIS 审中-公开

    公开(公告)号：US20170159075A1

    公开(公告)日：2017-06-08

    申请号：US15039221

    申请日：2014-11-25

    Applicant: GENOMATICA, INC.

    Inventor： Robin E. OSTERHOUT ,  Anthony P. BURGARD ,  Priti PHARKYA ,  Stefan ANDRAE

    IPC: C12P5/02 ,  C12P7/04 ,  C12N15/81 ,  C12P17/10 ,  C12P13/00 ,  C12N15/70 ,  C12P7/18 ,  C12P7/44

    Abstract: The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention.

    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF FUMARATE, MALATE, AND ACRYLATE
    37.
    发明申请
    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF FUMARATE, MALATE, AND ACRYLATE 审中-公开
    富马酸和丙烯酸的生物合成的微生物和方法

    公开(公告)号：US20160017382A1

    公开(公告)日：2016-01-21

    申请号：US14716767

    申请日：2015-05-19

    Applicant: Genomatica, Inc.

    Inventor： Mark J. BURK ,  Anthony P. BURGARD ,  Priti PHARKYA

    IPC: C12P7/46 ,  C12N9/88 ,  C12P7/40

    CPC classification number: C12P7/46 ,  C12N9/88 ,  C12P7/40

    Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.

    Abstract translation: 非天然存在的真核生物或原核生物包括当基因破坏降低酶的活性时，编码在赋予生物体中增加的富马酸盐，苹果酸或丙烯酸酯产生的酶的基因中发生的一种或多种基因破坏。 一个或多个基因破坏使丙烯酸酯的生产增加到生物体上。 生产丙烯酸酯的生物体具有丙烯酸酯途径，至少一种编码丙烯酸酯途径酶的外源核酸以足够的量表达以产生丙烯酸酯，丙烯酸酯途径包含脱羧酶。 产生富马酸盐，苹果酸盐或丙烯酸盐的方法包括培养这些生物体。

    MICROORGANISMS AND METHODS FOR PRODUCING BUTADIENE AND RELATED COMPOUNDS BY FORMATE ASSIMILATION
    38.
    发明申请
    MICROORGANISMS AND METHODS FOR PRODUCING BUTADIENE AND RELATED COMPOUNDS BY FORMATE ASSIMILATION 审中-公开
    微生物和通过正式评估生产丁二烯及相关化合物的方法

    公开(公告)号：US20150050708A1

    公开(公告)日：2015-02-19

    申请号：US14213806

    申请日：2014-03-14

    Applicant: Genomatica, Inc.

    Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Priti PHARKYA ,  Stefan ANDRAE

    IPC: C12P5/02 ,  C12N15/81 ,  C12P7/16 ,  C12N15/70 ,  C12P7/04 ,  C12P7/18

    CPC classification number: C12P5/026 ,  C12N15/52 ,  C12P7/04 ,  C12P7/16 ,  C12P7/18 ,  Y02E50/10 ,  Y02E50/343

    Abstract: Provided herein are non-naturally occurring microbial organisms having a formaldehyde fixation pathway and a formate assimilation pathway, which can further include a methanol metabolic pathway, a methanol oxidation pathway, a hydrogenase and/or a carbon monoxide dehydrogenase. These microbial organisms can further include a butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol pathway. Additionally provided are methods of using such microbial organisms to produce butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol.

    Abstract translation: 本文提供了具有甲醛固定途径和甲酸同化途径的非天然存在的微生物生物，其可以进一步包括甲醇代谢途径，甲醇氧化途径，氢化酶和/或一氧化碳脱氢酶。 这些微生物可进一步包括丁二烯，1,3-丁二醇，巴豆醇或3-丁烯-2-醇途径。 另外提供了使用这些微生物生产丁二烯，1,3-丁二醇，巴豆醇或3-丁烯-2-醇的方法。

    MICROORGANISMS AND METHODS FOR THE CO-PRODUCTION OF ISOPROPANOL WITH PRIMARY ALCOHOLS, DIOLS AND ACIDS
    39.
    发明申请
    MICROORGANISMS AND METHODS FOR THE CO-PRODUCTION OF ISOPROPANOL WITH PRIMARY ALCOHOLS, DIOLS AND ACIDS 审中-公开
    用主要醇，二醇和酸共同生产异丙酚的微生物和方法

    公开(公告)号：US20140377820A1

    公开(公告)日：2014-12-25

    申请号：US14167693

    申请日：2014-01-29

    Applicant: Genomatica, Inc.

    Inventor： Priti PHARKYA ,  Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Mark J. BURK ,  Jun SUN

    IPC: C12P7/40 ,  C12P7/18 ,  C12P7/04

    CPC classification number: C12P7/40 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18

    Abstract: The invention provides a non-naturally occurring microbial organism having n-propanol and isopropanol pathways, 1,4-butanediol (14-BDO) and isopropanol pathways, 1,3-butanediol (13-BDO) and isopropanol pathways or methylacrylic acid (MAA) and isopropanol pathways. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in each of the respective n-propanol, 14-BDO, 13-BDO or MAA and isopropanol pathways. The invention additionally provides a method for co-producing n-propanol and isopropanol, 14-BDO and isopropanol, 13-BDO and isopropanol or MAA and isopropanol. The method can include culturing an n-propanol and an isopropanol co-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an n-propanol, an isopropanol, a 14-BDO, a 13-BDO and/or a MAA pathway enzyme in a sufficient amount to produce each of the respective products, under conditions and for a sufficient period of time to produce each of the respective products.

    Abstract translation: 本发明提供了具有正丙醇和异丙醇途径的非天然存在的微生物生物，1,4-丁二醇（14-BDO）和异丙醇途径，1,3-丁二醇（13-BDO）和异丙醇途径或甲基丙烯酸（MAA ）和异丙醇途径。 微生物生物体含有至少一种外源核酸，其编码在各自的正丙醇，14-BDO，13-BDO或MAA和异丙醇途径中的酶。 本发明另外提供了共同生产正丙醇和异丙醇，14-BDO和异丙醇，13-BDO和异丙醇或MAA和异丙醇的方法。 该方法可以包括培养正丙醇和异丙醇共同生产的微生物，其中微生物生物体表达至少一种编码正丙醇，异丙醇，14-BDO，13-BDO和/或 足够量的MAA途径酶，以在条件下和足够的时间内产生各个产物中的每一种以产生各个产物。

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