公开(公告)号：US4192784A

公开(公告)日：1980-03-11

申请号：US873026

申请日：1978-01-27

Applicant: Eric Brown ,  Egisto Boschetti ,  Monique M. Corgier ,  Joel Touet

Inventor： Eric Brown ,  Egisto Boschetti ,  Monique M. Corgier ,  Joel Touet

IPC: C08F20/00 ,  B01D15/08 ,  C08F20/52 ,  C08F220/58 ,  C08L33/24 ,  C08L33/26 ,  C12N11/08 ,  C08L89/00 ,  C08F20/54 ,  C08L3/00

CPC classification number: C08F220/58 ,  C12N11/08 ,  Y10S524/916 ,  Y10S526/909 ,  Y10T428/2998

Abstract: Water-insoluble, tridimensional cross-linked random copolymers, which contain in copolymerized form:(a) 25% to 98% by weight of N-[tris(hydroxymethyl)methyl]acrylamide or N-[tris(hydroxymethyl)methyl]methacrylamide or a mixture of these two compounds;(b) 2% to 50% by weight of one or more monomers having several polymerizable ethylenic double bonds and one or more hydroxy groups, and free from NH.sub.2 or COOH groups; and(c) 0% to 50% by weight of one or more monomers having a polymerizable ethylenic double bond and one or more amino or carboxy groups. These copolymers may be used, in the form of aqueous gels, as supports in techniques of separation such as gell permeation chromatography and in techniques of immobilization of natural substances.

Abstract translation: 含有共聚形式的水不溶性三维交联无规共聚物：（a）25-98重量％的N- [三（羟甲基）甲基]丙烯酰胺或N- [三（羟甲基）甲基]甲基丙烯酰胺或 这两种化合物的混合物; （b）2重量％至50重量％的一种或多种具有几个可聚合的烯属双键和一个或多个羟基并且不含NH 2或COOH基团的单体; 和（c）0重量％至50重量％的一种或多种具有可聚合烯键式双键和一个或多个氨基或羧基的单体。 这些共聚物可以水凝胶的形式用作分离技术中的载体，例如凝胶渗透色谱法和固定天然物质的技术。

公开(公告)号：US4189370A

公开(公告)日：1980-02-19

申请号：US917549

申请日：1978-06-21

Applicant: Egisto Boschetti

Inventor： Egisto Boschetti

IPC: G01N27/447 ,  G01N27/26 ,  G01N27/28

CPC classification number: G01N27/44747

Abstract: A process for preparing plates of a new gel polymer, said plates being adapted to the electrophoresis separation of the seric or plasmatic lipoproteins under a stepped gradient. The gel polymers are prepared by radical polymerization of N-methylol-acrylamide and of a bifunctional allylic or acrylic compound causing cross-linking to yield a tridimensional configuration polymer. The polymerization may optionally be catalyzed by peroxides and/or ultraviolet radiation. Anionic polysaccharides containing only COOH groups may be added to assist migration of lipoproteins in the gel.

Abstract translation: 一种制备新凝胶聚合物的平板的方法，所述板适于在梯度梯度下电泳分离浆液或血浆脂蛋白。 凝胶聚合物通过N-羟甲基丙烯酰胺和双官能烯丙基或丙烯酸化合物的自由基聚合制备，引起交联以产生三维构型聚合物。 聚合可以任选地被过氧化物和/或紫外线辐射催化。 可以加入仅含有COOH基团的阴离子多糖以辅助凝胶中脂蛋白的迁移。

公开(公告)号：US09803184B2

公开(公告)日：2017-10-31

申请号：US13976557

申请日：2011-12-30

Applicant: Egisto Boschetti ,  Gérald Perret

Inventor： Egisto Boschetti ,  Gérald Perret

IPC: C07H21/00 ,  C12Q1/68 ,  C12N9/64 ,  B01J20/289 ,  C07H21/02 ,  C07H21/04 ,  C07K1/22 ,  B01J20/32

CPC classification number: C12N9/6437 ,  B01J20/289 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3278 ,  C07H21/02 ,  C07H21/04 ,  C07K1/22

Abstract: The invention relates to a method for immobilizing nucleic ligands including at least one reactive amine function, by grafting on an activated solid substrate, including a step of coupling said nucleic acids on said activated solid substrate having a pH of less than 6.

公开(公告)号：US20110111978A1

公开(公告)日：2011-05-12

申请号：US13004281

申请日：2011-01-11

Applicant: Egisto Boschetti ,  Lee Lomas

Inventor： Egisto Boschetti ,  Lee Lomas

IPC: C40B30/04 ,  C40B50/14 ,  B82Y5/00

CPC classification number: C07K1/047 ,  C07K1/22

Abstract: The present invention provides diverse chemical libraries bound to small particle with paramagnetic properties. Typically, the chemical structures comprise a plurality of different chemical moieties, the particles are paramagnetic and have a diameter between about 100 nm and about 10 microns, the chemical structures bound to each particular particle have substantially the same structure and the combinatorial library comprises at least 100,000 different chemical structures.

Abstract translation: 本发明提供了与具有顺磁性质的小颗粒结合的不同化学文库。 通常，化学结构包括多个不同的化学部分，颗粒是顺磁性的并且具有约100nm至约10微米之间的直径，与每个特定颗粒结合的化学结构具有基本上相同的结构，组合文库至少包含 100,000种不同的化学结构。

公开(公告)号：US07833625B2

公开(公告)日：2010-11-16

申请号：US11074591

申请日：2005-03-08

Applicant: Egisto Boschetti ,  Pier Giorgio Righetti

Inventor： Egisto Boschetti ,  Pier Giorgio Righetti

IPC: B32B5/16

CPC classification number: G01N27/44747 ,  B01D57/02 ,  B01J20/26 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3268 ,  B01J20/327 ,  B01J20/3274 ,  B01J20/3276 ,  B01J20/3282 ,  B01J20/3293 ,  B01J2220/54 ,  C07K1/16 ,  C07K1/24 ,  C07K1/28 ,  G01N27/44795 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2995 ,  Y10T428/2996 ,  Y10T428/2998

Abstract: Materials, apparatuses, methods, and systems for isolating and identifying mixture components on the basis of isoelectric point (“pI”). In one aspect, the materials are particles adapted to have a predetermined isoelectric point. The particles include a porous body into which a plurality of cavities extend. A substance of a predetermined isoelectric point is deposited in the cavities to provide thereby the particle's isoelectric value.

Abstract translation: 用于基于等电点（“pI”）分离和鉴定混合物组分的材料，装置，方法和系统。 在一个方面，材料是适于具有预定等电点的颗粒。 颗粒包括多个空腔延伸到其中的多孔体。 将预定等电点的物质沉积在空腔中，从而提供颗粒的等电位值。

公开(公告)号：US07815783B2

公开(公告)日：2010-10-19

申请号：US11073999

申请日：2005-03-08

Applicant: Egisto Boschetti ,  Lee O. Lomas ,  Pierre Girot

Inventor： Egisto Boschetti ,  Lee O. Lomas ,  Pierre Girot

IPC: G01N27/453 ,  B01D57/02

CPC classification number: G01N27/44704 ,  B01D57/02 ,  B01J20/3204 ,  B01J20/327 ,  B01J20/3272 ,  B01J20/3274 ,  B01J2220/54 ,  C07K1/24 ,  C07K1/34 ,  G01N27/44795

Abstract: Each embodiment includes a central sample reservoir and a plurality of satellite reservoirs. In a first embodiment, a first electrode in electrical contact with the central reservoir is charged and second electrodes in electrical contact with the satellite reservoirs are sequentially charged, thereby pI filtering molecules in the central reservoir into the satellite reservoirs. In a second embodiment, the central reservoir is configured to rotate so that molecules in a sample in the central reservoir are centrifugally pI-filtered into the satellite reservoirs. In a third embodiment, first and second electrodes proximate opposite first and second satellite reservoirs, respectively, are charged. Some molecules in a sample are pI filtered into the first and second satellite reservoirs. Third and fourth electrodes proximate opposite third and fourth satellite reservoirs, respectively, are then charged. Some molecules in a sample are pI filtered into the third and fourth satellite reservoirs.

Abstract translation: 每个实施例包括中央样品储存器和多个卫星储存器。 在第一实施例中，与中央储存器电接触的第一电极被充电，并且与卫星储存器电接触的第二电极被顺序地充电，从而将中央储存器中的分子过滤到卫星储存器中。 在第二实施例中，中央储存器被配置为旋转，使得中央储存器中的样品中的分子被离心地pI过滤到卫星储存器中。 在第三实施例中，分别与第一和第二卫星储存器相邻的第一和第二电极被充电。 样品中的一些分子被pI过滤到第一和第二卫星储层中。 然后分别靠近相对的第三和第四卫星储存器的第三和第四电极被充电。 样品中的一些分子被pI过滤到第三和第四卫星储层中。

公开(公告)号：US07348184B2

公开(公告)日：2008-03-25

申请号：US10626493

申请日：2003-07-23

Applicant: William E. Rich ,  Egisto Boschetti ,  Lee O. Lomas ,  Tai-Tung Yip

Inventor： William E. Rich ,  Egisto Boschetti ,  Lee O. Lomas ,  Tai-Tung Yip

IPC: G01N33/543

CPC classification number: C40B30/04 ,  G01N33/54306 ,  G01N33/6803

Abstract: This invention provides methods and materials for mapping interaction characteristics between components of a multicomponent biological complex. The methods involve capturing a multicomponent complex on a solid support and washing the support with a series of elution washes forming a gradient of solute concentrations, and determining whether a particular elution wash eluted a particular component.

Abstract translation: 本发明提供了用于映射多组分生物复合物的组分之间的相互作用特征的方法和材料。 该方法包括在固体支持物上捕获多组分复合物，并用洗脱洗涤洗涤洗脱洗涤洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱特定组分。

公开(公告)号：US20070015143A1

公开(公告)日：2007-01-18

申请号：US11531863

申请日：2006-09-14

Applicant: Egisto Boschetti ,  Pierre Girot

Inventor： Egisto Boschetti ,  Pierre Girot

IPC: C12Q1/70 ,  C12Q1/68 ,  G01N33/53 ,  C12M3/00

CPC classification number: B01J20/3255 ,  B01D15/1807 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3242 ,  B01J20/3285 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00702 ,  B01J2219/00725 ,  B01J2219/0074 ,  C07K1/165 ,  C07K1/22 ,  C40B40/10 ,  C40B80/00 ,  G01N33/54353 ,  G01N2001/045 ,  G01N2035/00158 ,  Y10S530/812 ,  Y10T436/25

Abstract: For certain mixed mode resins having anionic character, a ligand is joined to a solid support via a linkage that includes a mercapto-, ether- or amino-containing moiety. A suitable ligand comprises an aromatic group, a heteroaromatic group, or a heterocyclic group, optionally fused, that is sulfate-, sulfonate-, phosphonate- or phosphate-substituted and that is linked to such a moiety. These resins possess an anionic character under conditions prescribed for their use. Separation of a biological substance, such as a peptide or protein, can be accomplished with a resin of this type via a change in the pH of eluants, thereby effecting adsorption and desorption.

Abstract translation: 对于具有阴离子特性的某些混合模式树脂，配体通过包含巯基，醚或氨基部分的键连接到固体支持物上。 合适的配体包括芳族基团，杂芳族基团或杂环基团，任选地稠合，其是硫酸酯基，磺酸酯基，膦酸酯基或磷酸酯取代的，并且与该部分连接。 这些树脂在其使用条件下具有阴离子特性。 生物物质如肽或蛋白质的分离可以通过这种类型的树脂通过洗脱液的pH的变化来实现，从而进行吸附和解吸附。

公开(公告)号：US20060205010A1

公开(公告)日：2006-09-14

申请号：US10553923

申请日：2004-04-19

Applicant: Catherine Allioux ,  Egisto Boschetti ,  Virginie Brenac ,  Patrick Santambien ,  James Spencer

Inventor： Catherine Allioux ,  Egisto Boschetti ,  Virginie Brenac ,  Patrick Santambien ,  James Spencer

IPC: G01N33/53

CPC classification number: G01N33/567 ,  C07K1/22 ,  G01N33/543 ,  G01N33/6803 ,  G01N33/6842 ,  G01N33/6851

Abstract: The invention provides methods of determining the presence of host cell proteins in sample. The invention further provides methods of following the purification of target proteins. Kits and articles of manufacture are also provided.

Abstract translation: 本发明提供了确定样品中宿主细胞蛋白质存在的方法。 本发明还提供了追踪靶蛋白纯化的方法。 还提供了工具包和制品。

公开(公告)号：US20050158393A1

公开(公告)日：2005-07-21

申请号：US11030182

申请日：2005-01-07

Applicant: Phillippe Reb ,  Laurent Domas ,  Egisto Boschetti ,  Marie-Paule Leroy-Landercy

Inventor： Phillippe Reb ,  Laurent Domas ,  Egisto Boschetti ,  Marie-Paule Leroy-Landercy

IPC: A61K9/14 ,  A61K33/24 ,  A61K49/18 ,  A61P43/00

CPC classification number: A61K49/18

Abstract: The present invention relates to polymeric materials that are labeled with colloidal metals, preferably colloidal gold, to processes for producing the labeled polymeric material, and to methods of using the materials in prophylactic, therapeutic and cosmetic applications. Specifically, the invention relates to porous injectable and implantable microparticles, preferably microspheres, that are associated with colloidal metals such that the microparticles are visible or detectable under regular light, by radiological and/or magnetic resonance imaging techniques, or both. The microparticles having colloidal metals are particularly useful for embolization, dermal augmentation and tissue bulking, drug delivery, gene therapy, and other prophylactic, therapeutic or cosmetic medical applications.

Abstract translation: 本发明涉及用胶体金属（优选胶体金）标记用于生产标记的聚合物材料的方法的聚合物材料，以及在预防，治疗和美容应用中使用该材料的方法。 具体地，本发明涉及与胶体金属相关的多孔可注射和可植入的微粒，优选微球，使得微粒在常规光下是可见的或可检测的，通过放射和/或磁共振成像技术，或两者兼有。 具有胶体金属的微粒特别可用于栓塞，皮肤增生和组织膨胀，药物递送，基因治疗和其它预防，治疗或美容医学应用。