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    MODIFIED PEPTIDE VACCINE DERIVED FROM INFLUENZA M2
    31.
    发明申请
    MODIFIED PEPTIDE VACCINE DERIVED FROM INFLUENZA M2 有权
    来自流感M2的改性肽疫苗

    公开(公告)号：US20120244184A1

    公开(公告)日：2012-09-27

    申请号：US13392994

    申请日：2010-08-23

    申请人： Kazuyoshi Kaminaka ,  Chikateru Nozaki ,  Junichi Matsuda ,  Kiyoto Nishiyama

    发明人： Kazuyoshi Kaminaka ,  Chikateru Nozaki ,  Junichi Matsuda ,  Kiyoto Nishiyama

    IPC分类号： C07K14/11 ,  C12N5/10 ,  C07H21/04 ,  C12N1/19 ,  C12N15/63 ,  C07K16/12 ,  A61K39/145 ,  C12N1/21

    CPC分类号： A61K39/145 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/55505 ,  C07K14/005 ,  C12N2760/16122 ,  C12N2760/16134

    摘要： A modified peptide derived from matrix protein 2 (hereinafter also referred to as “M2”), one of surface layer proteins of influenza virus, and a method for utilization of the modified peptide are provided. A peptide (hereinafter also referred to as “M2eC peptide”) that is made up by inserting cysteine residue(s) into a peptide (hereinafter also referred to as “M2e”) consisting of 23 amino acid residues of from positions No. 2 to No. 24 of M2 in influenza virus type A, a fusion protein consisting of said modified peptide and a polypeptide, an influenza vaccine comprising said modified peptide or said fusion protein as an active ingredient, a device which can be delivered into the body comprising said influenza vaccine, a nucleic acid fragment consisting of a nucleotide sequence encoding the amino acid sequence of said modified peptide or said fusion protein, an expression vector in which said nucleic acid fragment is incorporated, a host in which said expression vector is introduced, and an antibody that has a protective effect against influenza virus.

    摘要翻译： 提供了衍生自基质蛋白2的修饰肽（以下也称为“M2”），流感病毒的表层蛋白之一和使用修饰肽的方法。 通过将半胱氨酸残基插入由位置2〜23的23个氨基酸残基组成的肽（以下也称为“M2e”）而构成的肽（以下也称为“M2eC肽” 流感病毒A型M2的第24号，由所述修饰肽和多肽组成的融合蛋白，包含所述修饰肽或所述融合蛋白作为活性成分的流感疫苗，可以递送到身体的装置，包括所述 流感疫苗，由编码所述修饰肽或所述融合蛋白质的氨基酸序列的核苷酸序列组成的核酸片段，其中加入所述核酸片段的表达载体，导入所述表达载体的宿主和 对流感病毒具有保护作用的抗体。

    METHOD FOR ENHANCING IMMUNE RESPONSE WITH PEPTIDE
    35.
    发明申请
    METHOD FOR ENHANCING IMMUNE RESPONSE WITH PEPTIDE 有权
    用于增强与肽的免疫应答的方法

    公开(公告)号：US20100291122A1

    公开(公告)日：2010-11-18

    申请号：US12596741

    申请日：2008-04-18

    申请人： Junichi Matsuda ,  Kazuyoshi Kaminaka ,  Chikateru Nozaki

    发明人： Junichi Matsuda ,  Kazuyoshi Kaminaka ,  Chikateru Nozaki

    IPC分类号： A61K39/00 ,  C07K14/435 ,  A61K38/17 ,  A61P25/28 ,  A61P37/04

    CPC分类号： A61K39/0007 ,  A61K31/711 ,  C07K14/4711

    摘要： An object of the present invention is to provide a safe and effective method for enhancing an immune response and a medicament for preventing or treating Alzheimer disease comprising amyloid β peptide that induces an enhanced immune response. An amyloid β peptide or a portion thereof with addition or insertion of cysteine and a method for enhancing an immune response using the peptide or a method for enhancing an immune response using the peptide together with an adjuvant. A medicament for preventing or treating Alzheimer disease comprising an amyloid β peptide or a portion thereof that induces an enhanced immune response. A DNA vaccine, that may have the same effect, comprising the gene encoding an amyloid β peptide or a portion thereof that induces an enhanced immune response with addition or insertion of cysteine.

    摘要翻译： 本发明的目的是提供一种用于增强免疫应答的安全有效的方法和用于预防或治疗阿尔茨海默氏病的药物，其包括淀粉样蛋白 诱导增强的免疫应答的肽。 淀粉样蛋白 肽或其部分，其中加入或插入半胱氨酸，以及使用肽增强免疫应答的方法或使用肽与佐剂一起增强免疫应答的方法。 一种用于预防或治疗阿尔茨海默病的药物，其包含淀粉样蛋白 肽或其部分诱导增强的免疫应答。 可能具有相同作用的DNA疫苗，其包含编码淀粉样蛋白的基因; 肽或其部分，其通过加入或插入半胱氨酸诱导增强的免疫应答。

    Process for producing human thrombin by gene modification technique
    36.
    发明授权
    Process for producing human thrombin by gene modification technique 失效
    通过基因修饰技术生产人凝血酶的方法

    公开(公告)号：US07635577B2

    公开(公告)日：2009-12-22

    申请号：US10482926

    申请日：2002-07-04

    申请人： Hiroshi Yonemura ,  Tajayuki Imamura ,  Hiroshi Nakatake ,  Kenji Soejima ,  Chikateru Nozaki

    发明人： Hiroshi Yonemura ,  Tajayuki Imamura ,  Hiroshi Nakatake ,  Kenji Soejima ,  Chikateru Nozaki

    IPC分类号： C12P21/04

    CPC分类号： C12N9/6424 ,  C12Y304/21005

    摘要： A genetic recombinant human thrombin is provided. Human thrombin is efficiently prepared by the genetic engineering technique comprising the steps: (1) culturing a transfectant animal cell transfected with an expression vector in which a gene encoding human prethrombin is incorporated to the downstream of a promoter so as to produce and accumulate prethrombin in culture supernatant and recovering the produced human prethrombin; (2) treating a solution containing human prethrombin recovered in step (1) with ecarin so as to convert human prethrombin into human thrombin; and (3) purifying the solution obtained after the above activation process to obtain purified human thrombin. The present invention allows for provision of human thrombin in a large scale in a safe and economical manner due to exclusion of blood-derived components.

    摘要翻译： 提供了基因重组人凝血酶。 通过基因工程技术有效制备人凝血酶，所述方法包括以下步骤：（1）培养用表达载体转染的转染细胞动物细胞，其中将编码人凝血酶原的基因并入启动子的下游，以产生并累积凝血酶原 培养上清液并回收生产的人凝血酶原; （2）用ecarin处理步骤（1）中回收的含有人凝血酶原的溶液，以将人凝血酶原转化为人凝血酶; （3）纯化上述活化处理后得到的溶液，得到纯化人凝血酶。 本发明允许以安全和经济的方式大规模提供人类凝血酶，因为排除了血液衍生的成分。

    Recombinant plasmid inserted with herpes simplex virus gene
    40.
    发明授权
    Recombinant plasmid inserted with herpes simplex virus gene 失效
    用HERPES SIMPLEX病毒基因插入的重组质粒

    公开(公告)号：US5059538A

    公开(公告)日：1991-10-22

    申请号：US896923

    申请日：1986-08-15

    申请人： Chikateru Nozaki ,  Hiroshi Nakatake ,  Yoichiro Kino ,  Tatsuo Eto ,  Keiichi Makizumi ,  Nobuya Ohtomo

    发明人： Chikateru Nozaki ,  Hiroshi Nakatake ,  Yoichiro Kino ,  Tatsuo Eto ,  Keiichi Makizumi ,  Nobuya Ohtomo

    IPC分类号： C12N15/09 ,  A61K39/00 ,  C07H21/04 ,  C07K14/035 ,  C12N15/00 ,  C12N15/38 ,  C12N15/81 ,  C12P21/02 ,  C12R1/19 ,  C12R1/865

    CPC分类号： C12N15/81 ,  C07K14/005 ,  A61K39/00 ,  C12N2710/16622 ,  Y10S435/942

    摘要： A novel recombinant plasmid inserted with a herpes simplex virus gene, which comprises a plasmid vector containing a yeast DNA sequence and an Escherichia coli DNA sequence and carrying a promoter region and a herpes simplex virus gN gene (HSVgB) gene) recombined thereto under control of the promoter, said HSVgB gene lacking an N-terminal portion of the gene including a signal sequence-encoding region and optionally further lacking the region downstream therefrom, such as a gB gene lacking a DNA sequence encoding the N-terminal 30 amino acids, and a gB gene lacking a DNA sequence encoding the N-terminal 83 amino acids. The recombinant plasmid is useful for the production of transformed yeast, which is useful for the production of HSVgB proteins suitable for producing HSV vaccine and diagnostic reagents for herpes simplex virus infections.

    摘要翻译： 一种插入单纯疱疹病毒基因的新型重组质粒，其包含含有酵母DNA序列和大肠杆菌DNA序列并携带启动子区域和单纯疱疹病毒gN基因（HSVgB）基因）的质粒载体， 启动子，所述HSVgB基因缺失包含信号序列编码区的基因的N末端部分，并且任选地进一步缺少其下游区域，例如缺乏编码N末端30个氨基酸的DNA序列的gB基因，以及 缺乏编码N-末端83个氨基酸的DNA序列的gB基因。 重组质粒可用于生产转化酵母，其可用于生产适用于生产HSV疫苗的HSVgB蛋白和用于单纯疱疹病毒感染的诊断试剂。