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68 条记录 (耗时 0.038 秒)

    Polynucleotide determination with selectable cleavage sites
    31.
    发明授权
    Polynucleotide determination with selectable cleavage sites 失效
    可选切割位点的多核苷酸测定

    公开(公告)号：US5118605A

    公开(公告)日：1992-06-02

    申请号：US251152

    申请日：1988-09-29

    申请人： Michael S. Urdea

    发明人： Michael S. Urdea

    IPC分类号： G01N33/50 ,  C07H21/00 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/58

    CPC分类号： C12Q1/683 ,  C07H21/00 ,  C12Q1/6813 ,  C12Q1/6823 ,  C12Q1/6827 ,  Y10S435/803 ,  Y10S435/81

    摘要： Novel methods for assaying a nucleic acid analyte are provided, which employ polynucleotides having oligonucleotide sequences substantially homologous to a sequence of interest in the analyte, where the presence or absence of hybridization at a predetermined stringency provides for the release of a label from a support. Particularly, various techniques are employed for binding a label to a support, whereupon cleavage of either a single or double strand, a label may be released from a support, where the release of the label can be detected as indicative of the presence of a particular oligonucleotide sequence in a sample. The method finds use in diagnosis of disease, genetic monitoring, and analysis of nucleic acid mixtures.

    摘要翻译： 提供了用于测定核酸分析物的新方法，其使用具有与分析物中感兴趣的序列基本上同源的寡核苷酸序列的多核苷酸，其中以预定的严格性存在或不存在杂交提供了标记从载体的释放。 特别地，使用各种技术将标签结合到载体上，因此单链或双链的切割可以从载体释放，其中标记的释放可以被检测为指示存在特定的 样品中的寡核苷酸序列。 该方法用于诊断疾病，遗传监测和核酸混合物的分析。

    Differential hybridization for relative quantification of variant populations
    34.
    发明授权
    Differential hybridization for relative quantification of variant populations 失效
    差异杂交用于变异种群的相对定量

    公开(公告)号：US5827648A

    公开(公告)日：1998-10-27

    申请号：US688088

    申请日：1996-07-29

    申请人： P. Scott Eastman ,  Michael S. Urdea ,  Janice A. Kolberg

    发明人： P. Scott Eastman ,  Michael S. Urdea ,  Janice A. Kolberg

    IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C12P19/34

    CPC分类号： C12Q1/703 ,  C12Q1/6827 ,  C12Q1/6851

    摘要： This invention relates to a method of measuring the relative populations of first and second variants of a target nucleotide sequence of a target genome in a sample utilizing an amplification step, followed by probing with first and second variant probes and a control probe. Specific embodiments include an assay to monitor the conversion of codon 215 for HIV-1 reverse transcriptase from wild type to mutant form.

    摘要翻译： 本发明涉及使用扩增步骤测量样品中目标基因组的靶核苷酸序列的第一和第二变异体的相对群体的方法，然后用第一和第二变体探针和对照探针进行探测。 具体实施方案包括监测HIV-1逆转录酶的密码子215从野生型到突变体形式的转化的测定。

    Process for immobilizing nucleic acid probes on polystyrene surfaces
    36.
    发明授权
    Process for immobilizing nucleic acid probes on polystyrene surfaces 失效
    将核酸探针固定在聚苯乙烯表面上的方法

    公开(公告)号：US5712383A

    公开(公告)日：1998-01-27

    申请号：US438639

    申请日：1995-05-10

    申请人： Patrick Sheridan ,  Chu-An Chang ,  Joyce Running ,  Michael S. Urdea

    发明人： Patrick Sheridan ,  Chu-An Chang ,  Joyce Running ,  Michael S. Urdea

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/70 ,  C12R1/92 ,  G01N33/53 ,  G01N33/547 ,  G01N33/566 ,  C07H21/02

    CPC分类号： C12Q1/6813 ,  C12Q1/6834

    摘要： Nucleic acid probes are immobilized on polystyrene surfaces such as the wells of microtiter plates for use in solution phase nucleic acid sandwich hybridization assays, particularly those using large branched DNA amplification multimers, by: (a) cleansing the surface by washing it sequentially with a strong acid, a strong base, and water, (b) passively adsorbing a polypeptide having primary amino groups onto the cleansed surface, and (c) covalently bonding the probe to the adsorbed polypeptide via a base-stable bifunctional crosslinking agent, and (d) subjecting the surface to conditions that simulate the hybridization conditions used in the assay.

    摘要翻译： 将核酸探针固定在聚苯乙烯表面上，例如微量滴定板的孔，用于溶液相核酸夹心杂交测定法，特别是使用大分支DNA扩增多聚体的那些;（a）通过以下方式顺序洗涤来清洗表面： 酸，强碱和水，（b）被动吸附具有伯氨基的多肽到清洁表面上，和（c）通过碱稳定的双功能交联剂将探针共价结合到吸附的多肽，和（d） 使表面经受模拟测定中使用的杂交条件的条件。

    N-4 modified pyrimidine deoxynucleotides and oligonucleotide probes synthesized therewith
    38.
    发明授权
    N-4 modified pyrimidine deoxynucleotides and oligonucleotide probes synthesized therewith 失效
    N-4修饰的嘧啶脱氧核苷酸和与其合成的寡核苷酸探针

    公开(公告)号：US5580731A

    公开(公告)日：1996-12-03

    申请号：US296793

    申请日：1994-08-25

    申请人： Chu-An Chang ,  Michael S. Urdea ,  Thomas Horn

    发明人： Chu-An Chang ,  Michael S. Urdea ,  Thomas Horn

    IPC分类号： C07H19/10 ,  C07H21/00 ,  C12Q1/68 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/70

    CPC分类号： C07H19/10 ,  C07H21/00 ,  Y02P20/55

    摘要： Novel N-4 modified pyrimidine analogs are provided having the structure (I) or (II) ##STR1## wherein: R.sup.1 is selected from the group consisting of hydrogen, acid-sensitive, base-stable blocking groups and acyl capping groups; R.sup.2 is lower alkyl; R.sup.3 is C.sub.1 -C.sub.12 alkylene containing 0 to 6 linkages selected from the group consisting of --0--, --S-- and --NH--; R.sup.4 is ##STR2## in which R.sup.9 is hydrogen or an optionally substituted aliphatic group; R.sup.10 and R.sup.11 are hydrocarbyl or together form a mono- or polyheterocyclic ring; R.sup.5 is hydrogen or lower alkyl; R.sup.6 is selected from the group consisting of hydrogen, methyl, bromo and iodo; R.sup.7 is C.sub.1 -C.sub.12 alkylene containing 0 to 6 linkages selected from the group consisting of --O--, --S-- and --NR.sup.12 -- wherein R.sup.12 is hydrogen or lower alkyl; and R.sup.8 is a protecting group that can be removed and replaced by reduction. Synthetic methods for preparing the compounds are provided as well, as are methods for making and using polynucleotide probes containing the novel pyrimidine analogs.

    摘要翻译： 提供具有结构（I）或（II）的新型N-4修饰的嘧啶类似物，其中：R1选自氢，酸敏感，碱稳定 封闭基团和酰基封端基团; R2是低级烷基; R3是含有0-6个选自-O - ， - S - 和-NH-的键的C1-C12亚烷基; R4是，其中R9是氢或任选取代的脂族基; R 10和R 11是烃基或一起形成单或多杂环; R5是氢或低级烷基; R 6选自氢，甲基，溴和碘; R7是含有0-6个选自-O - ， - S-和-NR12-​​的键的C1-C12亚烷基，其中R12是氢或低级烷基; R8是可以除去并被还原代替的保护基。 还提供了制备化合物的合成方法，以及制备和使用含有新型嘧啶类似物的多核苷酸探针的方法。