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    Ionic compounds containing the cationic meriquinone of a benzidine
    3.
    发明授权
    Ionic compounds containing the cationic meriquinone of a benzidine 失效
    含有联苯胺阳离子季戊四醇的离子化合物

    公开(公告)号：US4789630A

    公开(公告)日：1988-12-06

    申请号：US896677

    申请日：1986-08-20

    申请人： Will Bloch ,  Patrick J. Sheridan ,  Robert J. Goodson

    发明人： Will Bloch ,  Patrick J. Sheridan ,  Robert J. Goodson

    IPC分类号： G01N33/545 ,  C12Q1/28 ,  C12Q1/68 ,  G01N21/82 ,  G01N33/535 ,  G01N33/543 ,  G01N33/58 ,  G01N33/53

    CPC分类号： C12Q1/28 ,  C12Q1/68 ,  G01N33/581 ,  C12Q2326/12 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S435/96 ,  Y10S435/975

    摘要： Useful for visualizing biological materials in a solid phase, on a gel, or in a liquid phase is a solid salt of the meriquinone of benzidine or a substituted benzidine. An immobilized or dissolved complex of a polymeric anion and the meriquinone of benzidine or a substituted benzidine having controllable solubility may also be employed. Preferred are meriquinone salts and complexes of 3,3,5,5'-tetramethylbenzidine. For visualization, the benzidine or substituted benzidine is oxidized to its meriquinone at pH 3 to 7 in the presence of an effective anion or polymeric anion, an oxidation catalyst, and an effective amount of oxidant to form a solid salt or immobilized complex of the meriquinone under conditions where the meriquinone solubility lies below about 10.sup.-5 M.

    摘要翻译： 可用于在固相，凝胶或液相中可视化生物材料是联苯胺或取代的联苯胺的固醇的固体盐。 还可以使用固定或溶解的聚合阴离子的复合物和具有可控的溶解度的联苯胺或取代的联苯胺的金刚酮。 优选3,3,5,5'-四甲基联苯胺的季戊四醇盐和络合物。 为了可视化，联苯胺或取代的联苯胺在有效阴离子或聚合阴离子，氧化催化剂和有效量的氧化剂的存在下，在pH 3至7下被氧化成其三聚物，以形成固体盐或固定的复合物 在meriquinone溶解度低于约10 -5 M的条件下。

    Quantitative determination of a DNA polymerase and a test kit useful in same
    5.
    发明授权
    Quantitative determination of a DNA polymerase and a test kit useful in same 失效
    DNA聚合酶的定量测定和可用于其的测试试剂盒

    公开(公告)号：US5049490A

    公开(公告)日：1991-09-17

    申请号：US482137

    申请日：1990-02-20

    申请人： John W. H. Sutherland ,  Patrick J. Sheridan ,  Louis M. Mezei

    发明人： John W. H. Sutherland ,  Patrick J. Sheridan ,  Louis M. Mezei

    IPC分类号： C12N15/09 ,  C12N15/00 ,  C12Q1/48 ,  C12Q1/68

    CPC分类号： C12Q1/48

    摘要： A convenient method for the quantitative determination of a DNA polymerase includes contacting an aqueous test specimen suspected of containing the enzyme with the following: a single-stranded DNA template present in a concentration of at least about 10.sup.-8 molar bases, a DNA primer complementary to the template, a source of a metal polymerase cofactor, sufficient deoxyribonucleoside triphosphates to synthesize double-stranded DNA in the presence of the polymerase, and a colorimetric or fluorescent dye which is capable of providing a detectable signal when a primed single-stranded nucleic acid is converted to double-stranded DNA by the polymerase. The rate of signal generation is then measured and can be correlated with the level of DNA polymerase in the specimen using graphical or mathematical means. The results of this method are precise, having a covariance of less than about 10%. A test kit includes the reagents needed for carrying out this method.