公开(公告)号：US20050255564A1

公开(公告)日：2005-11-17

申请号：US10500834

申请日：2003-01-17

申请人： Takeshi Sakai ,  Hitomi Amarume ,  Katsushige Ikai ,  Ikunoshin Kato

发明人： Takeshi Sakai ,  Hitomi Amarume ,  Katsushige Ikai ,  Ikunoshin Kato

IPC分类号： C07H11/00 ,  C08B37/00 ,  C12N9/18 ,  C12N9/24 ,  C12N9/26 ,  C12P19/04 ,  C12P19/14 ,  C12P19/20 ,  C08B35/02

CPC分类号： C12N9/2408 ,  C07H11/00 ,  C08B37/0006 ,  C12N9/18 ,  C12N9/2402 ,  C12P19/04 ,  C12P19/14 ,  C12P19/20 ,  C12Y302/01051

摘要： An enzyme capable of degrading sulfated fucan originating in a brown alga of Fucales which is useful in sugar chain engineering; a process for producing this enzyme; an oligosaccharide obtained by treating sulfated fucan with the enzyme; and production thereof.

摘要翻译： 能够降解起源于Fucales褐藻的硫酸化岩藻糖的酶，其可用于糖链工程; 一种生产该酶的方法; 通过用该酶处理硫酸化的岩藻糖获得的寡糖; 及其生产。

公开(公告)号：US20050202425A1

公开(公告)日：2005-09-15

申请号：US10487097

申请日：2002-09-05

申请人： Harumi Ueno ,  Jun Tomono ,  Hiroaki Sagawa ,  Takeshi Sakai ,  Ikunoshin Kato

发明人： Harumi Ueno ,  Jun Tomono ,  Hiroaki Sagawa ,  Takeshi Sakai ,  Ikunoshin Kato

IPC分类号： C12N1/19 ,  C12N1/21 ,  C12N9/24 ,  C12N9/40 ,  C12N15/56 ,  C12P19/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/14 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/74

CPC分类号： C12N9/2465 ,  C12N9/2402 ,  C12P19/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/14 ,  G01N2333/924

摘要： A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.

摘要翻译： 编码具有消化硫酸化的赖氨酰半乳糖的活性的多肽的基因，其可用于糖链工程试剂，分析硫酸化岩藻糖多糖的结构并制备多糖的降解产物; 用于产生多肽的基因工程方法; 和通过该方法获得的多肽。

公开(公告)号：US06927289B2

公开(公告)日：2005-08-09

申请号：US10694352

申请日：2003-10-28

申请人： Takeshi Sakai ,  Hitomi Amarume ,  Takashi Kawai ,  Kaoru Kojima ,  Kazuo Shimanaka ,  Katsushige Ikai ,  Ikunoshin Kato

发明人： Takeshi Sakai ,  Hitomi Amarume ,  Takashi Kawai ,  Kaoru Kojima ,  Kazuo Shimanaka ,  Katsushige Ikai ,  Ikunoshin Kato

IPC分类号： C08B37/00 ,  C07H5/00

CPC分类号： C08B37/006

摘要： A smaller molecule obtainable by allowing a sulfated fucan-digesting enzyme which digests a novel sulfated polysaccharide derived from an alga belonging to Laminariales to act on a sulfated fucan, and a method for producing the same.

摘要翻译： 通过使来自属于层状藻酸盐的藻类的新型硫酸化多糖消化的硫酸化的岩藻糖消化酶作用于硫酸化的岩藻石上的小分子及其制造方法。

公开(公告)号：US06924097B1

公开(公告)日：2005-08-02

申请号：US09368572

申请日：1999-08-05

申请人： Toshiharu Ohba ,  Shuichi Takahashi ,  Yoshiko Anma ,  Kiyozo Asada ,  Ikunoshin Kato

发明人： Toshiharu Ohba ,  Shuichi Takahashi ,  Yoshiko Anma ,  Kiyozo Asada ,  Ikunoshin Kato

IPC分类号： A01H1/00 ,  C12N9/10 ,  C12N15/29 ,  C12N15/82 ,  C12P21/06 ,  C12Q1/68

CPC分类号： C12N15/8223 ,  C12N9/1051

摘要： A plant promoter capable of inducing the expression specifically at the site and stage wherein the reconstitution of plant cell wall xyloglucan is necessary, namely, a plant promoter originating in a gene which encodes an endo-xyloglucan transferase or a gene which encodes a substance having a function equivalent thereto; and a method for modifying the function of a plant with the use of the plant promoter and a method for cloning the plant promoter.

摘要翻译： 需要在植物细胞壁木葡聚糖重构的位点和阶段特异性诱导表达的植物启动子，即来源于编码木糖葡聚糖转移酶的基因的植物启动子或编码具有 功能等同于 以及使用植物启动子修饰植物功能的方法和用于克隆植物启动子的方法。

公开(公告)号：US20050153326A1

公开(公告)日：2005-07-14

申请号：US10995005

申请日：2004-11-23

申请人： Junko Yamamoto ,  Kazue Miyake ,  Hiroyuki Mukai ,  Fumitsugu Hino ,  Ikunoshin Kato

发明人： Junko Yamamoto ,  Kazue Miyake ,  Hiroyuki Mukai ,  Fumitsugu Hino ,  Ikunoshin Kato

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C12N9/22 ,  C12P19/34

CPC分类号： C12Q1/6844 ,  C12N15/10 ,  C12Q1/686 ,  C12Q2521/319 ,  C12Q2521/107

摘要： A method for synthesizing cDNA characterized by performing a reverse transcription reaction in the presence of an enzyme having a reverse transcriptional activity and another enzyme different from the former one which as a 3′-5′ exonuclease activity.

摘要翻译： 一种合成cDNA的方法，其特征在于在具有逆转录活性的酶的存在下进行逆转录反应，另一种不同于前者的3'-5'核酸外切酶活性的酶。

公开(公告)号：US06897042B2

公开(公告)日：2005-05-24

申请号：US10268229

申请日：2002-10-10

申请人： Masanori Takayama ,  Yoshiko Nomura ,  Ikunoshin Kato

发明人： Masanori Takayama ,  Yoshiko Nomura ,  Ikunoshin Kato

IPC分类号： C07K14/245 ,  C12N9/24 ,  C12N15/00 ,  C12N15/63 ,  C12N15/67 ,  C12N15/70 ,  C12N15/72 ,  C12P21/06 ,  C07H17/00

CPC分类号： C12N15/72 ,  C07K14/245 ,  C12N9/2471 ,  C12N15/00 ,  C12N15/635 ,  C12N15/67 ,  C12N15/70 ,  C12Y302/01023

摘要： A vector which is characterized in containing each of the following elements: (1) a promoter which shows its action in the host to be used; (2) regulatory region for regulating the action of the promoter of (1); and (3) a region which codes for the 5′-untranslated region derived from cold-shock protein gene mRNA or a region which codes for the region where substitution, deletion, insertion or addition of at least one base is applied to the said untranslated region.

摘要翻译： 一种载体，其特征在于含有以下元素：（1）在宿主中显示其作用的启动子; （2）调节（1）启动子作用的调节区; 和（3）编码来自冷休克蛋白基因mRNA的5'非翻译区的区域或编码至少一个碱基的取代，缺失，插入或添加的区域的区域，该区域应用于所述非翻译区 地区。

公开(公告)号：US20050048480A1

公开(公告)日：2005-03-03

申请号：US10333015

申请日：2001-07-18

申请人： Yasuhhiro Tsubosa ,  Kazuhiko Aoyagi ,  Hiroki Sasaki ,  Masaaki Terada ,  Junichi Mineno ,  Kiyozo Asada ,  Ikunoshin Kato

发明人： Yasuhhiro Tsubosa ,  Kazuhiko Aoyagi ,  Hiroki Sasaki ,  Masaaki Terada ,  Junichi Mineno ,  Kiyozo Asada ,  Ikunoshin Kato

IPC分类号： C12N15/12 ,  C12Q1/68 ,  C12Q1/6837 ,  C12Q1/6886

CPC分类号： C12Q1/6886 ,  C12Q1/6837 ,  C12Q2600/158

摘要： To provide a method for selecting a marker gene useful for cancer classification; a method for classifying cancer using the gene; a method for detecting cancer; a kit usable for the classification method or detection method; and a DNA array carrying the gene. According to the present invention, there can be obtained a gene, wherein expression of the above gene is altered independently from genes each of which expression is altered specifically during cell proliferation and expression level of the above gene is specifically altered depending on every type of cancer samples to be tested, whereby the classification or detection of cancer can be carried out conveniently and quickly without giving surgical treatment. Therefore, the present invention is useful for the diagnosis, the treatment, and the like of cancer.

摘要翻译： 提供选择用于癌症分类的标记基因的方法; 使用该基因分类癌症的方法; 一种检测癌症的方法; 可用于分类方法或检测方法的试剂盒; 和携带该基因的DNA阵列。 根据本发明，可以获得上述基因的表达独立于在细胞增殖期间特异性改变的基因的各基因的变化，并且根据每种类型的癌症特异性地改变上述基因的表达水平 待测试的样品，从而可以方便快捷地进行癌症的分类或检测，而无需外科治疗。 因此，本发明对癌症的诊断，治疗等有用。

公开(公告)号：US06548543B1

公开(公告)日：2003-04-15

申请号：US09763244

申请日：2001-02-20

申请人： Eiji Kobayashi ,  Hiromu Ohnogi ,  Hiroaki Sagawa ,  Takanari Tominaga ,  Eiji Nishiyama ,  Nobuto Koyama ,  Katsushige Ikai ,  Ikunoshin Kato

发明人： Eiji Kobayashi ,  Hiromu Ohnogi ,  Hiroaki Sagawa ,  Takanari Tominaga ,  Eiji Nishiyama ,  Nobuto Koyama ,  Katsushige Ikai ,  Ikunoshin Kato

IPC分类号： A61K31215

CPC分类号： A61K31/215 ,  A61K31/22 ,  C07C69/013 ,  C07C2601/10 ,  Y02A50/411

摘要： Remedies or preventives for diseases with a need for immunoregulation, diseases with a need for inhibition of inflammation, diseases with a need for regulation of tumor necrosis factor production, diseases with a need for regulation of fungal growth, diseases with a need for regulation of cell adhesion or disease with a deed for induction of heat-shock protein, which contain as the active ingredient at least one compound selected from among cyclopentenone derivatives represented by general formula [I], optically active isomers and salts thereof, wherein R1 and R2 are the same or different and each represents hydrogen, an aliphatic group, an aromatic group or an aromatic aliphatic group.

摘要翻译： 需要免疫调节的疾病的治疗或预防措施，需要抑制炎症的疾病，需要调节肿瘤坏死因子产生的疾病，需要调节真菌生长的疾病，需要调节细胞的疾病 含有作为活性成分的至少一种选自通式[I]表示的环戊烯酮衍生物的化合物，其光学活性异构体和盐，其中R 1和R 2为 相同或不同，各自表示氢，脂族基，芳基或芳族脂族基。

公开(公告)号：US06448048B1

公开(公告)日：2002-09-10

申请号：US09214146

申请日：1998-12-29

申请人： Jun Tomono ,  Akihiko Kita ,  Susumu Tsunasawa ,  Ikunoshin Kato

发明人： Jun Tomono ,  Akihiko Kita ,  Susumu Tsunasawa ,  Ikunoshin Kato

IPC分类号： C12N1564

CPC分类号： C12N15/102

摘要： A method for performing site-directed mutagenesis characterized in that the method includes the step of carrying out PCR by the use of a double-stranded DNA vector having one or more amber codons, the vector resulting from insertion of a target DNA fragment for site-directed mutagenesis, and at least two kinds of selection primers; and a kit for site-directed mutagenesis for use in the above method, characterized in that the kit includes amber codon reversion primers. According to the present invention, there can be provided a method for performing site-directed mutagenesis and a kit, which is useful for genetic engineering or protein engineering, more simply and rapidly. By using the method and the kit of the present invention, it is possible to efficiently obtain a mutation-introduced gene at the desired position by simply transforming a host with a PCR product obtained by PCR.

摘要翻译： 一种用于进行定点诱变的方法，其特征在于该方法包括通过使用具有一个或多个琥珀密码子的双链DNA载体进行PCR的步骤，所述双链DNA载体由插入靶DNA片段所产生的载体， 定向诱变和至少两种选择引物; 以及用于上述方法的用于定点诱变的试剂盒，其特征在于所述试剂盒包括琥珀密码子反转引物。 根据本发明，可以提供用于进行定点诱变的方法和用于遗传工程或蛋白质工程的试剂盒，其更简单和快速。 通过使用本发明的方法和试剂盒，通过用PCR获得的PCR产物简单地转化宿主，可以有效地获得所需位置的突变导入基因。

公开(公告)号：US06432664B1

公开(公告)日：2002-08-13

申请号：US09447223

申请日：1999-11-23

申请人： Takashi Okado ,  Kazutoh Takesako ,  Ikunoshin Kato

发明人： Takashi Okado ,  Kazutoh Takesako ,  Ikunoshin Kato

IPC分类号： C12Q118

CPC分类号： C07K14/40 ,  A61K38/00 ,  C07K14/37 ,  C07K16/14 ,  Y10S435/822 ,  Y10S435/911 ,  Y10S530/824

摘要： The invention provides an isolated gene coding for a protein regulating aureobasidin sensitivity; a process for cloning the gene with the use of the gene or a part of the same as a probe; a nucleic acid probe being hybridizable with the gene; an antisense DNA or RNA of the gene; a recombinant or transformant having the gene contained therein; an isolated protein regulating aureobasidin sensitivity and a process for producing the same by using the transformant; an antibody for the protein; a process for detecting the protein or the gene; and a process for screening an antimycotic by using the protein or the transformant. The invention is useful in the diagnosis and treatment of diseases including mycoses.

摘要翻译： 本发明提供编码调节金黄担子素敏感性蛋白质的分离基因; 使用该基因或其一部分作为探针克隆该基因的方法; 核酸探针可与该基因杂交; 该基因的反义DNA或RNA; 其中含有该基因的重组体或转化体; 调节金丝桃素敏感性的分离蛋白质及其使用该转化体的方法。 蛋白质的抗体; 检测蛋白质或基因的方法; 以及通过使用蛋白质或转化体筛选抗真菌药物的方法。 本发明可用于诊断和治疗包括真菌病的疾病。