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    Aligned and electrospun piezoelectric polymer fiber assembly and scaffold
    32.
    发明授权
    Aligned and electrospun piezoelectric polymer fiber assembly and scaffold 有权
    对称和静电压电聚合物纤维组装和支架

    公开(公告)号：US09005604B2

    公开(公告)日：2015-04-14

    申请号：US12969076

    申请日：2010-12-15

    申请人： Lisa A. Scott-Carnell ,  Emilie J. Siochi ,  Nancy M. Holloway ,  Kam W. Leong ,  Karina Kulangara

    发明人： Lisa A. Scott-Carnell ,  Emilie J. Siochi ,  Nancy M. Holloway ,  Kam W. Leong ,  Karina Kulangara

    IPC分类号： C12N11/08 ,  C12N13/00 ,  A61L27/16 ,  A61L27/38 ,  C12N5/00 ,  A61K35/12

    CPC分类号： C12N5/0068 ,  A61K35/12 ,  A61K35/28 ,  A61L27/16 ,  A61L27/3834 ,  C12N11/08 ,  C12N13/00 ,  C12N2529/00 ,  C12N2533/30 ,  C08L27/16

    摘要： A scaffold assembly and related methods of manufacturing and/or using the scaffold for stem cell culture and tissue engineering applications are disclosed which at least partially mimic a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct. In another aspect, the invention provides a method of manufacturing the assembly including the steps of providing a first pre-electroded substrate surface; electrospinning a first substantially aligned polymer fiber layer onto the first surface; providing a second pre-electroded substrate surface; electrospinning a second substantially aligned polymer fiber layer onto the second surface; and, retaining together the layered surfaces with a clamp and/or an adhesive compound.

    摘要翻译： 公开了制造和/或使用用于干细胞培养和组织工程应用的支架的支架组件和相关方法，其通过使用电活性材料提供生物化学，地形学，机械和电学提示，至少部分地模拟天然生物环境。 该组件包括至少一层基本对准的电纺聚合物纤维，其具有用于单独电压施加的操作连接。 细胞组织工程和/或干细胞分化的方法利用悬浮在细胞培养基中的细胞样品接种组合物，孵育并施加电压至一层或多层，从而产生细胞和/或组织构建体。 另一方面，本发明提供一种制造组件的方法，其包括以下步骤：提供第一预电镀基底表面; 将第一基本上排列的聚合物纤维层静电纺丝到第一表面上; 提供第二预电镀衬底表面; 将第二基本上排列的聚合物纤维层静电纺丝到第二表面上; 并且用夹具和/或粘合剂化合物将层压表面保持在一起。

    Biodegradable foams for cell transplantation
    37.
    发明授权
    Biodegradable foams for cell transplantation 失效
    可生物降解的泡沫用于细胞移植

    公开(公告)号：US5686091A

    公开(公告)日：1997-11-11

    申请号：US219017

    申请日：1994-03-28

    申请人： Kam W. Leong ,  Hungnan Lo ,  Sudhakar Kadiyala

    发明人： Kam W. Leong ,  Hungnan Lo ,  Sudhakar Kadiyala

    IPC分类号： A61F2/02 ,  A61F2/00

    CPC分类号： A61F2/022 ,  Y10T428/249955 ,  Y10T428/249958 ,  Y10T428/249978

    摘要： A structurally rigid biodegradable foam scaffold useful for cell transplantation is provided. The foam can be loaded with nutrients and/or drugs that elute from the foam during transplant to promote growth of the cells. The foam, which features a continuous network of pores, is fabricated using a novel method involving phase separation and consequent expansion upon sublimation of a liquid solvent, preferably naphthalene.

    摘要翻译： 提供了可用于细胞移植的结构刚性可生物降解泡沫支架。 泡沫可以装载在移植期间从泡沫中洗脱的营养物和/或药物以促进细胞的生长。 具有连续的孔孔网的泡沫体使用涉及液相溶剂（优选萘）升华的相分离和随后膨胀的新方法制造。

    Bioerodible articles useful as implants and prostheses having predictable degradation rates
    39.
    发明授权
    Bioerodible articles useful as implants and prostheses having predictable degradation rates 失效
    用作具有可预测降解速率的植入物和假体的生物蚀刻制品

    公开(公告)号：US4886870A

    公开(公告)日：1989-12-12

    申请号：US702168

    申请日：1985-02-15

    申请人： Patricia D'Amore ,  Kam W. Leong ,  Robert S. Langer

    发明人： Patricia D'Amore ,  Kam W. Leong ,  Robert S. Langer

    IPC分类号： A61L17/10 ,  A61L27/18 ,  A61L27/58 ,  C08G67/04

    CPC分类号： C08G67/04 ,  A61L17/10 ,  A61L27/18 ,  A61L27/58

    摘要： A novel series of articles useful as implants and prostheses and methods for their preparation and use are provided which utilize polyanhydride polymeric matrices as a general class of materials. These articles are biocompatible, non-inflammatory and degrade predictably into non-toxic residues after introduction in-vivo. The articles may be formed in any desired dimensions and configuration and may take specific shape as vascular or skin grafts, as biodegradable sutures or as orthopedic appliances such as bone plates and the like.

    摘要翻译： 提供了可用作植入物和假体的新型系列文章及其制备和使用方法，其利用聚酐聚合物基质作为一般材料类。 这些制品在生物相容性，非炎症性和体内引入后可预测地降解成无毒性残留。 制品可以以任何期望的尺寸和构型形成，并且可以具有特定形状作为血管或皮肤移植物，例如可生物降解的缝线或矫形器具如骨板等。

    Methods And Products For Delivering Biological Molecules To Cells Using Multicomponent Nanostructures
    40.
    发明申请
    Methods And Products For Delivering Biological Molecules To Cells Using Multicomponent Nanostructures 审中-公开
    使用多组分纳米结构向生物分子提供细胞的方法和产品

    公开(公告)号：US20080199531A1

    公开(公告)日：2008-08-21

    申请号：US11936574

    申请日：2007-11-07

    申请人： Aliasger Karimjee Salem ,  Kam W. Leong ,  Peter Charles Searson

    发明人： Aliasger Karimjee Salem ,  Kam W. Leong ,  Peter Charles Searson

    IPC分类号： A61K9/50 ,  A61K31/70 ,  A61K39/00

    CPC分类号： A61K9/5115 ,  A61K47/02 ,  A61K47/6923 ,  A61K47/6929 ,  B82Y30/00 ,  C12N15/87

    摘要： This invention is predicated on the present applicants' discovery that nanostructures comprising discrete regions of different composition can be used to deliver to a biological cell a desired combination of molecules in close proximity. Different molecules can be selectively bonded to discrete regions of different composition in sufficiently close physical relationship to enhance delivery or effectiveness within the cell. The preferred nanostructures are multicomponent nanorods. Important applications include delivery of missing DNA sequences for gene therapy and delivery of antigens or DNA encoding antigens for vaccination.

    摘要翻译： 本发明基于本申请人的发现，包括不同组成的离散区域的纳米结构可以用于向生物细胞递送非常接近的期望的分子组合。 不同分子可以以足够接近的物理关系选择性地键合到不同组成的离散区域，以增强细胞内的递送或有效性。 优选的纳米结构是多组分纳米棒。 重要的应用包括递送缺失的DNA序列用于基因治疗和递送抗原或DNA编码抗原用于疫苗接种。