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    System and Method for Performing G Protein Coupled Receptor (GPCR) Cell Assays Using Waveguide-Grating Sensors
    31.
    发明申请
    System and Method for Performing G Protein Coupled Receptor (GPCR) Cell Assays Using Waveguide-Grating Sensors 有权
    使用波导光栅传感器进行G蛋白偶联受体（GPCR）细胞测定的系统和方法

    公开(公告)号：US20090275074A1

    公开(公告)日：2009-11-05

    申请号：US12469054

    申请日：2009-05-20

    申请人： Ye Fang ,  Ann M. Ferrie ,  Norman H. Fontaine ,  Joydeep Lahiri ,  Po Ki Yuen

    发明人： Ye Fang ,  Ann M. Ferrie ,  Norman H. Fontaine ,  Joydeep Lahiri ,  Po Ki Yuen

    IPC分类号： C12Q1/02

    CPC分类号： G01N33/5041 ,  G01N21/4788 ,  G01N21/553 ,  G01N2333/726 ,  G01N2500/10

    摘要： The present invention includes a system and method that uses optical LID biosensors to monitor in real time agonist-induced GPCR signaling events within living cells. Particularly, the present invention includes a system and method for using an optical LID biosensor to screen compounds against a target GPCR within living cells based on the mass redistribution due to agonist-induced GPCR activation. In an extended embodiment, the present invention discloses different ways for self-referencing the optical LID biosensor to eliminate unwanted sensitivity to ambient temperature, pressure fluctuations, and other environmental changes. In yet another extended embodiment, the present invention discloses different ways for screening multiple GPCRs in a single type of cell or multiple GPCRs in multiple types of cells within a single medium solution. In still yet another extended embodiment, the present invention discloses different ways to confirm the physiological or pharmacological effect of a compound against a specific GPCR within living cells.

    摘要翻译： 本发明包括使用光学LID生物传感器实时监测活细胞内激动剂诱导的GPCR信号事件的系统和方法。 特别地，本发明包括使用光学LID生物传感器基于由于激动剂诱导的GPCR活化导致的质量再分布来筛选活细胞内的靶GPCR的化合物的系统和方法。 在扩展实施例中，本发明公开了用于自参考光学LID生物传感器以消除对环境温度，压力波动和其他环境变化的不期望的灵敏度的不同方式。 在又一个扩展的实施方案中，本发明公开了在单一培养基溶液中的多种类型的细胞中单种类型细胞或多种GPCR筛选多种GPCR的不同方式。 在又一个延伸的实施方案中，本发明公开了确定化合物对活细胞内特异性GPCR的生理或药理作用的不同方式。

    LABEL-FREE METHODS RELATED TO hERG POTASSIUM ION CHANNELS
    36.
    发明申请
    LABEL-FREE METHODS RELATED TO hERG POTASSIUM ION CHANNELS 审中-公开
    与无机磷离子通道相关的无标记方法

    公开(公告)号：US20130040331A1

    公开(公告)日：2013-02-14

    申请号：US13519246

    申请日：2010-12-16

    申请人： Ye Fang ,  Haiyan Sun

    发明人： Ye Fang ,  Haiyan Sun

    IPC分类号： C12Q1/02 ,  G01N27/26

    CPC分类号： G01N33/6872 ,  G01N2500/10

    摘要： Disclosed are methods to classify human ether-à-go-go related gene (hERG) ion channel modulators using label-free biosensors. Disclosed is the use of label-free resonant waveguide grating (RWG) biosensors to reveal the patterns of the DMR signals of hERG modulators across three types of cell lines (a native cell line endogenously expressing hERG, a native cell line without hERG and its engineered cell line stably expressed hERG), as well as the corresponding modulation index of the modulators molecules against a hERG activator acting on a panel of markers/cells, particularly the known hERG activator mallotoxin DMR signals in the two hERG expressing cell lines.

    摘要翻译： 公开了使用无标记生物传感器分类人类醚转运相关基因（hERG）离子通道调节剂的方法。 公开了使用无标记谐振波导光栅（RWG）生物传感器来显示三种类型的细胞系（内源性表达hERG的天然细胞系，没有hERG的天然细胞系的天然细胞系及其工程化的hERG调节剂的DMR信号的模式 细胞系稳定表达hERG），以及调节剂分子对作用于一组标志物/细胞的hERG激活剂的相应调节指数，特别是两种hERG表达细胞系中已知的hERG活化剂mallotoxin DMR信号。

    SPACED PROJECTION SUBSTRATES AND DEVICES FOR CELL CULTURE
    37.
    发明申请
    SPACED PROJECTION SUBSTRATES AND DEVICES FOR CELL CULTURE 审中-公开
    间隔投影基质和细胞培养装置

    公开(公告)号：US20100129908A1

    公开(公告)日：2010-05-27

    申请号：US12622079

    申请日：2009-11-19

    申请人： Ye Fang ,  Yi-Cheng Hsieh ,  Ling Huang ,  Ying Wei

    发明人： Ye Fang ,  Yi-Cheng Hsieh ,  Ling Huang ,  Ying Wei

    IPC分类号： C12N5/071

    CPC分类号： C12N5/067 ,  C12M25/02 ,  C12N2502/13 ,  C12N2533/54

    摘要： An article for culturing cells includes a substrate on which cells can be cultured. The substrate has a base surface. An array of projections extends from the base surface. The projections have a height of about 1 micrometer to about 100 micrometers, and have a gap distance along the major surface from center to center between neighboring projections of about 10 micrometers to 80 micrometers. A plurality of arrays of projections may extend from the surface with gaps in the base surface between the arrays. Hepatocytes cultures on such microprojection array substrates maintained in vivo like morphology and membrane polarity. Hepatocytes co-cultured with helper cells on such substrates tended to grow in the area of the arrays, while the helper cells tended to grow in the areas between the arrays.

    摘要翻译： 培养细胞的制品包括可以培养细胞的底物。 基板具有基面。 一组突起从基面延伸出来。 突起具有约1微米至约100微米的高度，并且在约10微米至80微米的相邻突起之间具有从中心到中心的主表面的间隙距离。 多个突起阵列可以从阵列之间的基部表面中的间隙从表面延伸。 在这样的微喷射阵列基板上的肝细胞培养物保持体内，如形态和膜极性。 与这种底物上的辅助细胞共培养的肝细胞趋向于在阵列区域中生长，而辅助细胞趋向于在阵列之间的区域中生长。

    Method and device for protein delivery into cells
    39.
    发明授权
    Method and device for protein delivery into cells 失效

    公开(公告)号：US08293484B2

    公开(公告)日：2012-10-23

    申请号：US12893389

    申请日：2010-09-29

    申请人： Ye Fang ,  Fang Lai ,  Laurent A. G. Picard ,  Brian L. Webb

    发明人： Ye Fang ,  Fang Lai ,  Laurent A. G. Picard ,  Brian L. Webb

    IPC分类号： G01N33/53

    CPC分类号： C12N5/0068 ,  C12N2503/00 ,  C12N2535/10

    摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.

    Method and Device for Protein Delivery into Cells
    40.
    发明申请
    Method and Device for Protein Delivery into Cells 失效

    公开(公告)号：US20120083428A1

    公开(公告)日：2012-04-05

    申请号：US12893389

    申请日：2010-09-29

    申请人： Ye Fang ,  Fang Lai ,  Laurent A.G. Picard ,  Brian L. Webb

    发明人： Ye Fang ,  Fang Lai ,  Laurent A.G. Picard ,  Brian L. Webb

    IPC分类号： C40B40/02 ,  C40B50/06

    CPC分类号： C12N5/0068 ,  C12N2503/00 ,  C12N2535/10

    摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.