Haloperoxidases with altered pH profiles
    33.
    发明授权
    Haloperoxidases with altered pH profiles 有权
    具有改变的pH曲线的过氧化氢酶

    公开(公告)号:US06221821B1

    公开(公告)日:2001-04-24

    申请号:US09271778

    申请日:1999-03-18

    IPC分类号: C12N908

    摘要: Variants of a parent vanadium-containing haloperoxidase, which variant has haloperoxidase activity and an altered pH optimum and comprises a mutation in a position corresponding to at least one of the following positions: R490A, L, I, Q, M, E, D; A399G; F397N, Y, E, Q; P395A, S; R360A, L, I, Q, M, E, D; K353Q, M; S402A, T, V, S; D292L; A501S; W350F, Y; V495A, T, V, S; K394 A, L, I, Q, M, E, D; wherein the parent haloperoxidase has the amino acid sequence given in SEQ ID No. 1, or the parent haloperoxidase has an amino acid sequence which is at least 80% homologous to SEQ ID No. 1.

    摘要翻译: 母体含钒卤代过氧化物酶的变体,其变体具有卤代过氧化物酶活性和改变的pH最佳值,并且在与以下至少一个位置相对应的位置包含突变:R490A,L,I,Q,M,E,D; A399G; F397N,Y,E,Q; P395A,S; R360A,L,I,Q,M,E,D; K353Q,M; S402A,T,V,S; D292L; A501S; W350F,Y; V495A,T,V,S; K394 A,L,I,Q,M,E,D; 其中母体卤代过氧化物酶具有SEQ ID No.1所示的氨基酸序列,或者母体过氧化氢酶具有与SEQ ID No.1至少80%同源的氨基酸序列。

    Laccase mutants
    34.
    发明授权
    Laccase mutants 有权
    漆酶突变体

    公开(公告)号:US06218170B1

    公开(公告)日:2001-04-17

    申请号:US09518901

    申请日:2000-03-06

    申请人: Allan Svendsen

    发明人: Allan Svendsen

    IPC分类号: C12N120

    摘要: The present invention relates to laccase mutants with increased oxidation potential and/or changed pH optimum and/or altered mediator pathway and/or altered O2/OH− pathway.

    摘要翻译: 本发明涉及具有增加的氧化电位和/或改变的pH最佳和/或改变的介体途径和/或改变的O 2 / OH途径的漆酶突变体。