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    METHOD FOR QUANTIFYING HUMAN DNA USING AN INTERNAL CONTROL
    41.
    发明申请
    METHOD FOR QUANTIFYING HUMAN DNA USING AN INTERNAL CONTROL 有权
    使用内部控制量化人类DNA的方法

    公开(公告)号：US20140147843A1

    公开(公告)日：2014-05-29

    申请号：US14000092

    申请日：2012-02-20

    申请人： Francesca Di Pasquale ,  Holger Engel ,  Sascha Strauss ,  Nicola Jo Thel Well

    发明人： Francesca Di Pasquale ,  Holger Engel ,  Sascha Strauss ,  Nicola Jo Thel Well

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6879 ,  C12Q1/6851 ,  C12Q1/6888 ,  C12Q2600/16 ,  C12Q2600/166 ,  C12Q2525/151 ,  C12Q2545/101 ,  C12Q2561/113

    摘要： The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.

    摘要翻译： 本发明涉及用于量化和/或检测样品中基因组的一种或多种核酸的方法，其中在扩增反应中，（i）第一个核酸被扩增，被扩增的基因座是多拷贝基因座 （MCL），其中所述基因座与SEQ ID NO：1的序列具有至少80％的序列同一性。 并且其中多拷贝基因座在至少两个不同染色体上具有拷贝，（ii）作为内部对照（IC）加入的第二个核酸也被扩增，并且（iii） 确定来自扩增第一核酸的扩增产物的量。

    NOVEL COMBINATION OF FLUORESCENT DYES FOR THE DETECTION OF NUCLEIC ACIDS
    43.
    发明申请
    NOVEL COMBINATION OF FLUORESCENT DYES FOR THE DETECTION OF NUCLEIC ACIDS 有权
    用于检测核酸的荧光染料的新组合

    公开(公告)号：US20140087956A1

    公开(公告)日：2014-03-27

    申请号：US14091804

    申请日：2013-11-27

    申请人： QIAGEN GMBH

    发明人： Werner Brabetz ,  Cornelia Weber

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  C12Q1/6846 ,  C12Q2563/107 ,  C12Q2537/143

    摘要： The present invention relates to combinations of fluorescent dyes used in molecular biology, particularly in multiplex PCR. In particular, the present invention relates to a combination of dyes for amplification reactions, wherein at least four different dyes are used, wherein the first dye is 5-FAM or 6-FAM or a blend thereof, the second dye is selected from the group consisting of DY-530, HEX, CAL Fluor Orange 560 and ATTO 532, the third dye is selected from the group consisting of ATTO 550, DY-555 and DY-556, the fourth dye is selected from the group consisting of ROX, DY-510XL and ATTO 565, and optionally a fifth dye is selected from the group consisting of DY 632 and DY-520XL.

    摘要翻译： 本发明涉及分子生物学中特别是多重PCR中使用的荧光染料的组合。 特别地，本发明涉及用于扩增反应的染料的组合，其中使用至少四种不同的染料，其中第一种染料是5-FAM或6-FAM或其共混物，第二种染料选自 由DY-530，HEX，CAL Fluor Orange 560和ATTO 532组成，第三种染料选自ATTO 550，DY-555和DY-556，第四种染料选自ROX，DY -510XL和ATTO 565，以及任选的第五染料选自DY 632和DY-520XL。

    MODIFIED HYBRIDIZATION PROBES
    44.
    发明申请
    MODIFIED HYBRIDIZATION PROBES 审中-公开
    改性杂交探针

    公开(公告)号：US20140057263A1

    公开(公告)日：2014-02-27

    申请号：US14006794

    申请日：2012-03-21

    申请人： Holger Engel ,  Alexander Azzawi ,  Francesca Di Pasquale

    发明人： Holger Engel ,  Alexander Azzawi ,  Francesca Di Pasquale

    IPC分类号： C12Q1/68

    摘要： The invention relates to a method for detecting a nucleic acid of an organism in a composition, comprising the steps of, (i) amplifying the nucleic acid to be detected, (ii) during or after amplification, hybridizing to said nucleic acid to be detected a first probe that comprises an abasic site additionally optionally carrying a detectable label, (iii) wherein the position of the abasic site corresponds to a position in said nucleic acid to be detected, known to have a polymorphism in said organism, and wherein said nucleic acid is detected if hybridization occurs.

    摘要翻译： 本发明涉及一种用于检测组合物中生物体的核酸的方法，其包括以下步骤：（i）扩增要检测的核酸，（ii）在扩增期间或扩增后与待检测的核酸杂交 第一探针，其包含另外任选携带可检测标记的脱碱基位点，（iii）其中所述无碱基位点的位置对应于所述待检测核酸中的位置，已知在所述生物体中具有多态性，并且其中所述核酸 如果发生杂交，则检测到酸。

    Method for isolating and purifying nucleic acids
    45.
    发明授权
    Method for isolating and purifying nucleic acids 失效
    分离和纯化核酸的方法

    公开(公告)号：US08624020B2

    公开(公告)日：2014-01-07

    申请号：US13061928

    申请日：2009-09-02

    申请人： Ralf Himmelreich ,  Sabine Werner

    发明人： Ralf Himmelreich ,  Sabine Werner

    IPC分类号： C07H21/00

    CPC分类号： C12N15/1006

    摘要： The present invention relates to a method for the isolation and purification of nucleic acids by elution of nucleic acids from nucleic acid-containing samples, and biological materials, using a wash buffer comprising an alcohol having 1 to 3 carbon atoms and at least one further solvent selected from the group consisting of alkane diols and alkane triols having 2 to 6 carbon atoms, monocarboxylic acid esters and dicarboxylic acid diesters having 2 to 6 carbon atoms in the acidic component and 1 to 4 carbon atoms in the alcoholic component; (poly)ethylene glycols and ether derivatives and ester derivatives thereof, and poly(4-styrene sulfonic acid-co-maleic acid) sodium salt solution. The present invention further relates to a kit for carrying out the method of the invention.

    摘要翻译： 本发明涉及通过使用含有1至3个碳原子的醇和至少一种其它溶剂的洗涤缓冲液从核酸样品和生物材料中洗脱核酸来分离和纯化核酸的方法 选自烷烃二醇和具有2至6个碳原子的烷烃三醇，在酸性组分中具有2至6个碳原子的二羧酸二酯和醇组分中的1至4个碳原子的单羧酸酯和二羧酸二酯; （聚）乙二醇及其醚衍生物及其酯衍生物，以及聚（4-苯乙烯磺酸 - 共 - 马来酸）钠盐溶液。 本发明还涉及用于实施本发明方法的试剂盒。

    REAGENT FOR THE DISRUPTION OF CELL MATERIAL HAVING A COMPLETELY INTEGRATED INTERNAL STANDARD
    47.
    发明申请
    REAGENT FOR THE DISRUPTION OF CELL MATERIAL HAVING A COMPLETELY INTEGRATED INTERNAL STANDARD 审中-公开
    具有完整的内部标准的电池材料的破坏试剂

    公开(公告)号：US20130236900A1

    公开(公告)日：2013-09-12

    申请号：US13825487

    申请日：2011-09-23

    申请人： Dirk Heckel ,  Katharina Beller

    发明人： Dirk Heckel ,  Katharina Beller

    IPC分类号： G01N1/30

    CPC分类号： G01N1/30 ,  C12N1/06 ,  C12N1/066 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q2545/101 ,  C12Q2563/149

    摘要： The present invention relates to a reagent for the disruption of cell material, containing an internal standard that is completely integrated into the reagent for control and evaluation of the completeness of disruption of the cell material and subsequent steps, comprising a step selected from sample preparation, extraction, enrichment, isolation, purification, reverse transcription, amplification and detection of the cell components obtained from the disrupted cells, or a combination of a plurality or all of these steps.

    摘要翻译： 本发明涉及一种用于破坏细胞材料的试剂，其含有完全整合到用于控制和评价细胞材料破坏的完整性的试剂中的内标物和随后的步骤，包括选自样品制备， 提取，富集，分离，纯化，逆转录，扩增和检测从破碎的细胞获得的细胞组分，或这些步骤的多个或全部的组合。

    METHOD FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS
    48.
    发明申请
    METHOD FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS 有权
    核酸的同位素扩增方法

    公开(公告)号：US20130224799A1

    公开(公告)日：2013-08-29

    申请号：US13816081

    申请日：2011-08-09

    申请人： Christian Korfhage

    发明人： Christian Korfhage

    IPC分类号： C12Q1/68 ,  C12N11/04

    CPC分类号： C12Q1/6844 ,  C12N11/04 ,  C12P19/34 ,  C12Q1/6848 ,  C12Q2547/107 ,  C12Q2527/125 ,  C12Q2527/107 ,  C12Q2531/119 ,  C12Q2549/101

    摘要： A method is disclosed for improved isothermal amplification of nucleic acids comprising the step of release of an essential component from a matrix under predetermined conditions. Furthermore, the invention relates to a kit comprising mesophilic enzyme and a matrix with embedded essential components for isothermal amplification. A composition comprising a matrix and a mesophilic enzyme and a method for embedding a mesophilic enzyme are disclosed as well.

    摘要翻译： 公开了用于改进核酸等温扩增的方法，其包括在预定条件下从基质中释放必需组分的步骤。 此外，本发明涉及包含嗜温酶和具有用于等温扩增的嵌入必需成分的基质的试剂盒。 还公开了包含基质和嗜温酶的组合物以及嵌入嗜温酶的方法。

    METHOD FOR ISOLATING A TARGET NUCLEIC ACID INCLUDING SMALL TARGET NUCLEIC ACIDS WITH HIGH YIELD
    50.
    发明申请
    METHOD FOR ISOLATING A TARGET NUCLEIC ACID INCLUDING SMALL TARGET NUCLEIC ACIDS WITH HIGH YIELD 审中-公开
    用于分离靶向核酸的方法，包括具有高YIELD的小目标核酸

    公开(公告)号：US20130164825A1

    公开(公告)日：2013-06-27

    申请号：US13820228

    申请日：2011-09-02

    申请人： Vera Holländer ,  Gabriele Christoffel ,  Martin Schlumpberger

    发明人： Vera Holländer ,  Gabriele Christoffel ,  Martin Schlumpberger

    IPC分类号： C07H1/08

    CPC分类号： C12N15/1006 ,  C12N15/1017

    摘要： The present invention pertains to a method for isolating a target nucleic acid including small target nucleic acids from a sample, said method comprising at least the following steps a) binding at least a portion of the target nucleic acid including small target nucleic acids to a nucleic acid binding solid phase comprised in a column by passing the sample through said column, b) performing an enzymatic and/or chemical treatment on the nucleic acid binding solid phase while the target nucleic acid is bound to said solid phase, c) collecting at least a portion of the small target nucleic acids released from the solid phase during said treatment of step b) as flow-through, d) contacting said flow-through which comprises small target nucleic acids mixed with a recovery solution with a nucleic acid binding solid phase for binding the contained small target nucleic acids to said nucleic acid binding solid phase, e) optionally performing an elution. The present invention results in a considerable increase in the yield of small target nucleic acids in the isolated target nucleic acid because it allows to efficiently capture and recover small target nucleic acids.

    摘要翻译： 本发明涉及从样品中分离包含小靶核酸的靶核酸的方法，所述方法至少包括以下步骤：a）将包含小靶核酸的靶核酸的至少一部分结合至核酸 通过使样品通过所述柱而包含在柱中的酸结合固相，b）对核酸结合固相进行酶和/或化学处理，同时靶核酸与所述固相结合，c）至少收集 在步骤b）的所述处理期间从固相中释放的一部分小目标核酸作为流通，d）使所述流通接触，所述流过包含与回收溶液混合的小目标核酸与核酸结合固相 用于将所含的小靶核酸与所述核酸结合固相结合，e）任选地进行洗脱。 本发明导致分离的靶核酸中小靶核酸的产量显着增加，因为其允许有效捕获和回收小靶核酸。