公开(公告)号：US12169034B2

公开(公告)日：2024-12-17

申请号：US16077779

申请日：2017-02-21

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer ,  Sudheer Mygapula ,  Nachiket Karmarkar ,  Sasikumar Arumugam ,  Bashkar Hv ,  Ajit S. Vernekar ,  Nityananda Maiya ,  Prashanth Hosabettu Mohan ,  Sasi Kumar Nutalapati ,  Sameera Simha Hindupur ,  Shashidhar Hedge

IPC: F16K7/06 ,  B01D61/12 ,  B01D61/22 ,  F16K7/07 ,  G05D16/20

Abstract: The invention discloses a method of controlling a transmembrane pressure (TMP) in a crossflow filtration apparatus, comprising the steps of: a) providing a crossflow filtration apparatus comprising a pump (21) fluidically connected via a retentate compartment (33) of a crossflow filter (32) and a length of flexible tubing to a regulator valve (1; 101) acting on the flexible tubing, wherein the regulator valve is a high precision pinch valve. The apparatus further comprises a first pressure transducer (22) adapted to measure a pressure between the retentate compartment and the regulator valve, a second pressure transducer (39) adapted to measure a pressure between the pump and the retentate compartment and a third pressure transducer (40) adapted to measure a pressure at a permeate outlet (36) of the crossflow filter, and a control unit (23) electrically or electromagnetically connected to said regulator valve, the first, second and third pressure transducers and optionally to the pump; b) pumping a liquid with the pump via the second pressure transducer, through the retentate compartment via the length of flexible tubing, the first pressure transducer and the regulator valve; c) controlling the regulator valve with the control unit, such that a TMP calculated by formula (I) TMP=(Pinlet+Poutlet)/2−Pperm corresponds to a predetermined TMP value or to an interval between two predetermined TMP values, wherein Pinlet is a pressure reading from the second pressure transducer, Poutlet is a pressure reading from the first pressure transducer and Pperm is a pressure reading from the third pressure transducer.

公开(公告)号：US12135276B2

公开(公告)日：2024-11-05

申请号：US16464778

申请日：2017-11-30

Applicant: Cytiva Sweden AB

Inventor： Hanno Ehring ,  Mats Rimmo

IPC: G01N21/01 ,  G02B6/36

Abstract: An optical measuring device (1; 1′; 1″; 41; 61) configured for being connected in a flow path, said optical measuring device (1; 1′; 1″; 41; 61) comprising:—a first and a second flow path connector (5, 7; 5′, 7′; 5″, 7″);—at least one flow cell part (3; 3a, 3b; 3a, 3b, 3c, 3d) provided in between the first and second flow path connectors (5, 7; 5′, 7′; 5″, 7″) such that a flow in a flow path in which the optical measuring device (1; 1′; 1″; 41; 61) can be arranged will flow through the first flow path connector (5; 5′; 5″), the at least one flow cell part (3; 3a, 3b; 3a, 3b, 3c, 3d) and through the second flow path connector (7; 7′; 7″); and—at least one releasable connection device (9) arranged for releasably connecting the at least one flow cell part (3; 3a, 3b; 3a, 3b, 3c, 3d) in between the first and second flow path connectors (5, 7; 5′, 7′; 5″, 7″).

公开(公告)号：US20240361282A1

公开(公告)日：2024-10-31

申请号：US18770467

申请日：2024-07-11

Applicant: Cytiva Sweden AB

Inventor： Daniel Salomonsson ,  Mats Lundkvist

IPC: G01N30/60 ,  B01D15/20 ,  B01D15/22

CPC classification number: G01N30/6082 ,  B01D15/206 ,  B01D15/22

Abstract: Disclosed is a chromatographic separation column assembly (100) comprising a column cylinder (120) having a cylinder wall (126) including an inner wall surface (124) partially defining a column volume (50), and a column component (110/130) insertable into to the cylinder (120), wherein at least an edge region (113/133) of the column component is intended to be in contact or adjacent a part of the inner wall surface (124) in use, the edge region and the contacting or adjacent part of inner wall surface each being formed from a compatible heat fusible material or materials, and wherein at least the cylinder wall in the area of the contacting or adjacent part of the inner wall is formed from a material which allows transmission of the light energy needed to cause said fusing of the adapter plate edge region to the inner wall surface.

公开(公告)号：US20240279637A1

公开(公告)日：2024-08-22

申请号：US18693116

申请日：2022-09-30

Applicant: Cytiva Sweden AB

Inventor： Hans Blom ,  Markus Pitkanen ,  Linda Hagman ,  Annika Morrison

IPC: C12N15/10 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12N15/1003 ,  C12M23/14 ,  C12M41/12 ,  C12M41/26 ,  C12M47/06

Abstract: A method, a system and a use of a single use mixing bag for performing alkaline lysis, neutralisation and clarification of bacterial cells for extraction of plasmid DNA. The method comprises providing a suspension of bacterial cells and providing alkaline lysis solution into a single use mixing bag for performing an alkaline lysis of said bacterial cells, wherein said single use mixing bag comprises an integrated mixer. The method comprises furthermore mixing of the content in the single use mixing bag by the integrated mixer during the alkaline lysis and stopping the alkaline lysis by the addition of a neutralisation solution into the single use mixing bag and clarifying the content in the single use mixing bag after the alkaline lysis has been performed by the addition of a flocculate lifting agent into the single use mixing bag, whereby at least two phases are separated inside the single use mixing bag, whereby one of said phases is a neutralised and clarified alkaline solution comprising plasmid DNA. The neutralised and clarified alkaline solution is removed from the single use mixing bag and filtered.

公开(公告)号：US12066375B2

公开(公告)日：2024-08-20

申请号：US16464759

申请日：2017-11-30

Applicant: Cytiva Sweden AB

Inventor： Hanno Ehring ,  Mats Lundkvist ,  David Carl Martin Bergman ,  Andreas Marcstrom

IPC: G01N21/05 ,  G01N21/03 ,  G01N21/85

CPC classification number: G01N21/05 ,  G01N21/03 ,  G01N21/0303 ,  G01N21/85 ,  G01N2201/0668 ,  G01N2201/08

Abstract: The invention relates to an optical flow cell comprising a housing forming an enclosed and elongated fluid channel arranged along a first axis, an inlet arranged to connect a first outer surface area of the housing to a first end of the fluid channel and an outlet arranged to connect a second outer surface area to a second end of the fluid channel, a first light guide and a second light guide concentrically arranged along a second axis and on opposite side walls of the fluid channel. The invention further relates to a corresponding method to produce an optical flow cell.

公开(公告)号：US12019058B2

公开(公告)日：2024-06-25

申请号：US17938618

申请日：2022-10-06

Applicant: Cytiva Sweden AB

Inventor： Jens Widehammar ,  Key Hyckenberg ,  Orjan Grelsson ,  Lars Mattsson ,  Kjell Karlsson

IPC: G01N30/86 ,  G01N30/02

CPC classification number: G01N30/8658 ,  G01N2030/027

Abstract: The present invention relates to a liquid chromatography system configured to operate with at least one column and configured for purification of a sample comprising a target product using a predefined process. The liquid chromatography system comprises a controller configured to: control the operation of the chromatography system to run the predefined process; retrieve column data accessible from a data storage, the column data being specific to each column; and adapt at least one process parameter of the predefined process for each column based on column data. Whereby the predefined process is adapted to each column to obtain the target product and maintain the performance of the liquid chromatography system.

公开(公告)号：US11938418B2

公开(公告)日：2024-03-26

申请号：US15533591

申请日：2015-12-15

Applicant: Cytiva Sweden AB

Inventor： Karol Maciej Lacki

IPC: B01D15/16 ,  B01D15/18 ,  B01D15/20 ,  B01D15/38 ,  G01N30/42 ,  G01N30/88 ,  G01N30/56

CPC classification number: B01D15/163 ,  B01D15/1807 ,  B01D15/1871 ,  B01D15/20 ,  B01D15/3809 ,  G01N30/42 ,  G01N30/88 ,  G01N30/56 ,  G01N2030/889

Abstract: Certain embodiments of the invention provides a method for monitoring level of saturation of a chromatography media in a column, which method comprises measuring a first pressure at the inlet of an unloaded column; measuring a second pressure at the inlet from a loaded column; and comparing the first and second pressure measurement to determine the level of saturation of the chromatography media. Embodiments of the invention also provide related methods for controlling a chromatography system and methods for controlling a periodic counter current chromatography system, as well as a chromatography system suitable for use with the novel methods.

公开(公告)号：US20240076605A1

公开(公告)日：2024-03-07

申请号：US18272249

申请日：2022-02-01

Applicant: CYTIVA SWEDEN AB

Inventor： ANDREAS AXÉN ,  HELENA VESTERBERG ,  MAGNUS WETTERHALL ,  CAMILLA ESTMER NILSSON

IPC: C12M1/36 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12M41/48 ,  C12M29/10 ,  C12M29/24 ,  C12M41/12 ,  C12M41/26 ,  C12M41/30 ,  C12M41/46 ,  C12M47/10

Abstract: The present invention relates to a method for controlling process parameters in a bioreactor when producing a target product with a pre-determined critical to quality, CTQ, profile. The method comprises: monitoring (S20) at least one product quality attribute, PQA, for the target product; identifying (S30) trends and/or deviations by comparing the monitored at least one PQA to the CTQ profile of the target product; and controlling (S40) process parameters of the bioreactor based on the identified trends and/or deviations to maintain the target product within the CTQ profile.

公开(公告)号：US20240018952A1

公开(公告)日：2024-01-18

申请号：US18465810

申请日：2023-09-12

Applicant: Cytiva Sweden AB

Inventor： Andreas Torbjorn Lundin ,  Klas Marteleur ,  Mats Lundkvist ,  Andreas Marcstrom ,  David Gronowitz

IPC: F04B1/047 ,  F04B43/02

CPC classification number: F04B1/047 ,  F04B43/026 ,  F04B43/02

Abstract: A multiple cavity reciprocating positive displacement pump comprising plural pump cavities each including at least one pair of one-way valves the at least one pair including an inlet valve and an outlet valve, the pump being characterised in that respective inlet valves are in fluid communication with a common inlet and respective outlet valves are in fluid communication with a common outlet. The multiple cavities may include diaphragm arranged in a radial configuration for more uniform pressure output.

公开(公告)号：US11862335B2

公开(公告)日：2024-01-02

申请号：US18147546

申请日：2022-12-28

Applicant: Cytiva Sweden AB

Inventor： Key Hyckenberg ,  Kjell Karlsson ,  Stefan Willehadson ,  Orjan Bo Grelsson

IPC: G08B21/18 ,  G06K19/077 ,  G06F9/445 ,  G06F9/455 ,  G16H40/67 ,  G06F16/955 ,  G06F3/0483 ,  G06F3/04842 ,  H04L67/025 ,  H04L67/125

CPC classification number: G16H40/67 ,  G06F3/0483 ,  G06F3/04842 ,  G06F16/9558 ,  H04L67/025 ,  H04L67/125

Abstract: A method, system, gateway and computer program for remote controlling and monitoring of at least one instrument, for example a bioproces sing instrument. The system can comprise: at least one instrument to be controlled and/or monitored; at least one instrument server connected to the at least one instrument, said instrument server comprising an instrument control software; at least one gateway connected to the at least one instrument server; a transferring means provided in the at least one instrument server, said transferring means being arranged to receive information from the at least one connected instrument and forward said information to the at least one gateway.