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    Method of and apparatus for the purification of nucleic acids using immobilized metal-ligand complex
    44.
    发明申请
    Method of and apparatus for the purification of nucleic acids using immobilized metal-ligand complex 审中-公开
    使用固定化金属 - 配体复合物纯化核酸的方法和设备

    公开(公告)号：US20060275815A1

    公开(公告)日：2006-12-07

    申请号：US11446795

    申请日：2006-06-05

    Applicant: Chang-eun Yoo ,  Sang-hyun Peak ,  Sook-young Kim ,  Jong-myeon Park

    Inventor： Chang-eun Yoo ,  Sang-hyun Peak ,  Sook-young Kim ,  Jong-myeon Park

    IPC: C12Q1/68 ,  C07H21/04

    CPC classification number: C07H21/04 ,  C12N15/1006

    Abstract: Provided is a method of purifying nucleic acids using a metal-ligand complex, the method comprising: immobilizing the metal-ligand complex on a solid support; bringing a sample containing the nucleic acid into contact with the immobilized complex on the solid support to bind the nucleic acid to the complex; and adding a solution containing a chelate capable of removing the metal to elute the nucleic acid bound to the complex. The nucleic acids can be efficiently purified using a metal-ligand complex interacting with a base of the nucleic acid instead of interacting with a phosphoric acid of the nucleic acid to selectively bind the nucleic acid, and using a chelator capable of removing the metal to elute the nucleic acid.

    Abstract translation: 提供了使用金属 - 配体络合物纯化核酸的方法，所述方法包括：将所述金属 - 配体复合物固定在固体支持物上; 使含有核酸的样品与固体支持物上的固定化复合物接触以将核酸结合到复合物上; 并加入含有能除去金属的螯合物的溶液以洗脱与络合物结合的核酸。 可以使用与核酸的碱基相互作用的金属 - 配体复合物来有效纯化核酸，而不是与核酸的磷酸相互作用以选择性地结合核酸，并且使用能够去除金属洗脱的螯合剂 核酸。

    Hybridization system using the control of pump and valves in closed system
    45.
    发明申请
    Hybridization system using the control of pump and valves in closed system 失效
    混合系统使用封闭系统中的泵和阀的控制

    公开(公告)号：US20060154360A1

    公开(公告)日：2006-07-13

    申请号：US11295843

    申请日：2005-12-06

    Applicant: Soo-suk Lee ,  Sung-ouk Jung ,  Chang-eun Yoo ,  Hun-joo Lee ,  Christopher Ko ,  Nam Huh

    Inventor： Soo-suk Lee ,  Sung-ouk Jung ,  Chang-eun Yoo ,  Hun-joo Lee ,  Christopher Ko ,  Nam Huh

    IPC: C12M1/34

    CPC classification number: B01L7/00 ,  B01F13/0059 ,  B01F13/0222 ,  B01L3/5027 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/14 ,  B01L2300/1822 ,  B01L2400/0487 ,  G01N2035/00544

    Abstract: Provided is a hybridization system for hybridizing a biochip including: a chamber device including at least a hybridization chamber including a support for a biochip and a first cover having a sample inlet; an agitation device including: two air channels connected to ends of the hybridization chamber; two valves disposed in the air channels; an integrated air channel to which the two air channels are connected; and an air pump disposed in the integrated air channel; and a washing and drying device including: a flow channel connected to one of the two air channels through a branched valve; a flow pump disposed in the flow channel; and a buffer inlet disposed opposite the flow channel.

    Abstract translation: 提供了一种用于杂交生物芯片的杂交系统，包括：至少包括一个包括用于生物芯片的支撑物的杂交室的腔室装置和具有样品入口的第一盖子; 一种搅拌装置，包括：两个连接到杂交室端部的空气通道; 设置在空气通道中的两个阀; 两个空气通道连接的集成空气通道; 以及设置在所述集成空气通道中的空气泵; 以及洗涤干燥装置，包括：流路，其通过分支阀连接到所述两个空气通道中的一个; 设置在流路中的流量泵; 以及与流动通道相对设置的缓冲器入口。

    Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer
    47.
    发明申请
    Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer 失效
    使用固定化水凝胶或PEG-水凝胶共聚物分离和纯化核酸的方法

    公开(公告)号：US20060134675A1

    公开(公告)日：2006-06-22

    申请号：US11297783

    申请日：2005-12-07

    Applicant: Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

    Inventor： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

    IPC: C12Q1/68 ,  C07H21/04

    CPC classification number: C07H21/04

    Abstract: Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.

    Abstract translation: 提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。 该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。 因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。 此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

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