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67 条记录 (耗时 0.034 秒)

    Receptor polypeptides and their production and uses
    41.
    发明授权
    Receptor polypeptides and their production and uses 失效
    受体多肽及其生产和用途

    公开(公告)号：US06455262B1

    公开(公告)日：2002-09-24

    申请号：US08125065

    申请日：1993-09-21

    申请人： Edward T. Cox ,  Jennie P. Mather ,  Mary B. Sliwkowski ,  Teresa K. Woodruff

    发明人： Edward T. Cox ,  Jennie P. Mather ,  Mary B. Sliwkowski ,  Teresa K. Woodruff

    IPC分类号： G01N3353

    CPC分类号： C07K14/71 ,  C07K14/51 ,  C07K14/575

    摘要： An isolated TGF-&bgr; supergene family (TSF) receptor polypeptide is provided. This polypeptide preferably is an inhibin/activin receptor polypeptide and has at least 75% sequence identity with the mature human inhibin/activin receptor sequence. Also provided is a method for purifying TGF-&bgr; supergene family members such as inhibin or activin using the polypeptide, and a method for screening for compounds with TGF-&bgr; supergene family member activity by contacting the compound with the polypeptide and detecting if binding has occurred and the compound is active.

    摘要翻译： 提供了分离的TGF-β超基因家族（TSF）受体多肽。 该多肽优选是抑制素/激活素受体多肽，并且与成熟的人抑制素/激活素受体序列具有至少75％的序列同一性。 还提供了使用多肽纯化TGF-β超基因家族成员如抑制素或激活素的方法，以及通过使化合物与多肽接触并检测是否发生结合而筛选具有TGF-β超基因家族成员活性的化合物的方法 并且化合物是活性的。

    Cell arrays and the uses thereof
    42.
    发明授权
    Cell arrays and the uses thereof 有权
    电池阵列及其用途

    公开(公告)号：US06406840B1

    公开(公告)日：2002-06-18

    申请号：US09466011

    申请日：1999-12-17

    申请人： Ronghao Li ,  Jennie P. Mather

    发明人： Ronghao Li ,  Jennie P. Mather

    IPC分类号： C12N502

    CPC分类号： C40B30/04 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/0052 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01J2219/00673 ,  B01J2219/00689 ,  B01J2219/00702 ,  B01J2219/0072 ,  C40B60/14 ,  G01N1/36 ,  G01N33/6845

    摘要： The present invention provides cell arrays comprising a plurality of tubes containing populations of cells that are immobilized therein. The arrays are particularly useful for conducting comparative cell-based analyses. Specifically, the subject arrays allow protein-protein interactions to be studied in multiple types of cell simultaneously. The arrays also support simultaneous detection of the differential expression of a target polynucleotide in a multiplicity of cell types derived from multiple subjects. The subject arrays further permit high throughput screening for candidate modulators of a signal transduction pathway of interest. Further provided by the invention are kits, computer-implemented methods and systems for conducting the comparative cell-based analyses.

    摘要翻译： 本发明提供了包含多个管的细胞阵列，其中含有固定在其中的细胞群。 阵列特别适用于进行基于细胞的比较分析。 具体来说，主题阵列允许在多种类型的细胞中同时研究蛋白质 - 蛋白质相互作用。 阵列还支持同时检测衍生自多个受试者的多种细胞类型中靶多核苷酸的差异表达。 受试者阵列还允许感兴趣的信号转导途径的候选调节剂的高通量筛选。 本发明进一步提供的是用于进行基于比较细胞的分析的试剂盒，计算机实施的方法和系统。

    Method for culturing recombinant cells
    43.
    发明授权
    Method for culturing recombinant cells 失效
    培养重组细胞的方法

    公开(公告)号：US06399331B2

    公开(公告)日：2002-06-04

    申请号：US09728342

    申请日：2000-11-30

    申请人： Jennie P Mather ,  Axel Ullrich

    发明人： Jennie P Mather ,  Axel Ullrich

    IPC分类号： C12P2102

    CPC分类号： C07K14/59 ,  C07K14/79

    摘要： A method for culturing a recombinant host cell comprising: determining a polypeptide factor for a polypeptide factor-dependent host cell; transforming said host cell with nucleic acid encoding said polypeptide factor; transforming the host cell with nucleic acid encoding a desired protein; and, culturing the transformed host cells in a medium lacking the polypeptide factor.

    摘要翻译： 一种用于培养重组宿主细胞的方法，包括：确定多肽因子依赖性宿主细胞的多肽因子; 用编码所述多肽因子的核酸转化所述宿主细胞; 用编码所需蛋白质的核酸转化宿主细胞; 并且在缺乏多肽因子的培养基中培养转化的宿主细胞。

    Method of treating a nervous system injury with cultured schwann cells
    45.
    发明授权
    Method of treating a nervous system injury with cultured schwann cells 失效
    用培养的雪旺细胞治疗神经系统损伤的方法

    公开(公告)号：US6033660A

    公开(公告)日：2000-03-07

    申请号：US438862

    申请日：1995-05-10

    申请人： Jennie P. Mather ,  Ronghao Li ,  Jian Chen

    发明人： Jennie P. Mather ,  Ronghao Li ,  Jian Chen

    IPC分类号： A61K35/12 ,  C12N5/079 ,  C12N5/06

    CPC分类号： C12N5/0622 ,  A61K35/12 ,  C12N2500/90 ,  C12N2501/195 ,  C12N2501/40 ,  C12N2501/998

    摘要： A method for enhancing the survival and/or proliferation of Schwann cells (especially human Schwann cells) in cell culture is disclosed which involves culturing the cells in serum free culture medium comprising gas6 and other mitogenic agents, such as heregulin and forskolin. The culturing step is generally preceded by a pre-incubation period wherein nerve tissue comprising the Schwann cells is cultured under appropriate conditions and for a period of time such that demyelination occurs. The isolated Schwann cells can be used as cellular prostheses to treat patients with nervous system injuries. The invention also provides a cell culture medium for culturing Schwann cells.

    摘要翻译： 公开了一种用于增强细胞培养物中雪旺细胞（尤其是人雪旺细胞）的存活和/或增殖的方法，其涉及在包含气体6和其它有丝分裂剂（例如鸟氨酸和毛喉素）的无血清培养基中培养细胞。 培养步骤通常在预孵育期之前，其中包含雪旺细胞的神经组织在合适的条件下培养一段时间以使脱髓鞘发生。 孤立的施旺细胞可用作细胞假体来治疗神经系统损伤的患者。 本发明还提供了用于培养雪旺氏细胞的细胞培养基。

    Rse receptor activation
    46.
    发明授权
    Rse receptor activation 失效
    Rse受体激活

    公开(公告)号：US5955420A

    公开(公告)日：1999-09-21

    申请号：US438864

    申请日：1995-05-10

    申请人： Jian Chen ,  R. Glenn Hammonds ,  Paul J. Godowski ,  Melanie R. Mark ,  Jennie P. Mather ,  Ronghao Li

    发明人： Jian Chen ,  R. Glenn Hammonds ,  Paul J. Godowski ,  Melanie R. Mark ,  Jennie P. Mather ,  Ronghao Li

    IPC分类号： A61K38/00 ,  C07K14/745 ,  C12N1/21 ,  C12N15/12 ,  A61K38/18 ,  C07K14/475

    CPC分类号： C07K14/745 ,  A61K38/00

    摘要： An activator of the Rse receptor protein tyrosine kinase has been identified which is encoded by growth arrest-specific gene 6 (gas6). Accordingly, the present invention provides a method of activating the Rse receptor by exposing a cell comprising the Rse receptor to exogenous gas6 polypeptide. Moreover, the present invention is directed to a method for enhancing the survival, proliferation or differentiation of a cell comprising a Rse receptor by exposing the cell to exogenous gas6 polypeptide. The types of cells which can be treated according to the method include glial cells such as Schwann cells.

    摘要翻译： 已经鉴定了由生长停滞特异性基因6（gas6）编码的Rse受体蛋白酪氨酸激酶的激活剂。 因此，本发明提供了通过将包含Rse受体的细胞暴露于外源性气体6多肽来激活Rse受体的方法。 此外，本发明涉及通过将细胞暴露于外源性气体6多肽来增强包含Rse受体的细胞的存活，增殖或分化的方法。 根据该方法可以治疗的细胞类型包括神经胶质细胞，例如施旺细胞。

    Method of isolating lung cell line
    48.
    发明授权
    Method of isolating lung cell line 失效
    分离肺细胞系的方法

    公开(公告)号：US5364785A

    公开(公告)日：1994-11-15

    申请号：US60466

    申请日：1993-05-11

    申请人： Jennie P. Mather ,  Penelope E. Roberts

    发明人： Jennie P. Mather ,  Penelope E. Roberts

    IPC分类号： C07K14/475 ,  C07K14/785 ,  C12N5/071 ,  C12N15/85 ,  C12N5/00 ,  C12N15/00 ,  C12P21/02 ,  C12Q1/00

    CPC分类号： C12N15/85 ,  C07K14/475 ,  C07K14/785 ,  C12N5/0688 ,  C12N2500/25 ,  C12N2500/84 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2503/02

    摘要： A novel bronchial or bronchiolar epithelial cell from normal neonatal rat lung has been isolated, established and maintained for multiple passages in the absence of serum, without undergoing crisis or senescence. By careful manipulation of the nutritional/hormonal microenvironment we have been able to select, from a heterogeneous population, a single epithelial cell type which can maintain highly differentiated features in vitro. This cell type has characteristics of bronchiolar epithelial cells. A clonal line, RL-65, has been selected and observed for more than 2 years in continuous culture. It has been characterized by ultrastructural, morphological and biochemical criteria.

    摘要翻译： 已经分离出来自正常新生大鼠肺的新型支气管或细支气管上皮细胞，在不存在血清的情况下建立和维持多次传代，而不经历危机或衰老。 通过仔细操纵营养/激素微环境，我们已经能够从异质群体中选择能在体外保持高度分化特征的单一上皮细胞类型。 该细胞类型具有细支气管上皮细胞的特征。 克隆系RL-65在连续培养中被选择并观察了2年以上。 其特征在于超微结构，形态和生化标准。

    Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
    49.
    发明授权
    Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins 失效
    培养中国仓鼠卵巢细胞以改善重组蛋白产生的方法

    公开(公告)号：US5122469A

    公开(公告)日：1992-06-16

    申请号：US592107

    申请日：1990-10-03

    申请人： Jennie P. Mather ,  Mary C. Tsao

    发明人： Jennie P. Mather ,  Mary C. Tsao

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0037 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/40

    摘要： A method for culturing mammalian cells to improve production of recombinant proteins is provided. The culture media contain high levels of selected amino acids and may be supplemented with various growth factors and trace elements. In addition, the media are optionally serum-free. The media are preferably used for suspension culturing of Chinese hamster ovary cells at high densities.

    摘要翻译： 提供了培养哺乳动物细胞以改善重组蛋白质生产的方法。 培养基含有高水平的选择性氨基酸，可补充各种生长因子和微量元素。 此外，培养基任选地不含血清。 培养基优选以高密度悬浮培养中国仓鼠卵巢细胞。