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    Composition and method for production of transformed cells
    45.
    发明授权
    Composition and method for production of transformed cells 有权
    用于生产转化细胞的组合物和方法

    公开(公告)号：US07820634B2

    公开(公告)日：2010-10-26

    申请号：US11799724

    申请日：2007-05-02

    申请人： Stephen F. Badylak ,  Jeffrey Bonadio ,  Sherry Voytik

    发明人： Stephen F. Badylak ,  Jeffrey Bonadio ,  Sherry Voytik

    IPC分类号： A01N43/04 ,  A61K31/70 ,  A61F13/00 ,  A61K35/37 ,  A61K35/38 ,  C12N15/00

    CPC分类号： A61K48/0025 ,  A61F2/28 ,  A61F2002/2835 ,  A61F2002/30062 ,  A61F2002/30677 ,  A61F2210/0004 ,  A61K35/38 ,  A61K48/00 ,  A61L27/3629

    摘要： A composition useful for the production of transformed eukaryotic cells is described. The composition comprises submucosal tissue and a nucleic acid sequence. The nucleic acid sequence is typically recombinant DNA including gene(s) encoding for one or more biofunctional proteins. The submucosal tissue component of the present composition comprises the tunica submucosa of vertebrate intestine delaminated from the tunica muscularis and at least the luminal portion of the tunica mucosa. Injection or implantation of the composition into a host induces the formation of transformed cells capable of expressing gene(s) encoded by the nucleic acid sequence.

    摘要翻译： 描述了可用于生产转化真核细胞的组合物。 组合物包含粘膜下组织和核酸序列。 核酸序列通常是包含编码一种或多种生物功能蛋白质的基因的重组DNA。 本发明组合物的粘膜下组织组分包括从肌层剥离的脊椎动物肠的粘膜下膜和至少中膜粘膜的腔内部分。 将组合物注入或植入宿主中诱导能够表达由核酸序列编码的基因的转化细胞的形成。

    Vascularization Enhanced Graft Constructs
    47.
    发明申请
    Vascularization Enhanced Graft Constructs 有权
    血管化增强移植物构建

    公开(公告)号：US20090324681A1

    公开(公告)日：2009-12-31

    申请号：US12557176

    申请日：2009-09-10

    申请人： Stephen F. BADYLAK

    发明人： Stephen F. BADYLAK

    IPC分类号： A61F2/00 ,  A61K35/12

    CPC分类号： A61L27/3886 ,  A61K35/34 ,  A61L27/3604 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3813 ,  A61L27/3826 ,  A61L27/383

    摘要： A tissue graft construct for use in repairing diseased or damaged tissues is provided. The tissue graft construct comprises a matrix composition selected from the group consisting of liver basement membrane and extracts and hydrolysates thereof, and processed collagen from vertebrate non-submucosal sources, added endothelial cells, and at least one additional preselected, exogenous population of cells which enhance the initiation of vessel-like structures in the grant. The preselected population of cells can be a population of non-keratinized or keratinized epithelial cells or a population of mesodermally derived cells selected from the group consisting of fibroblasts, smooth muscle cells, skeletal muscle cells, cardiac muscle cells, multi-potential progenitor cells, pericytes, osteogenic cells, and any other suitable cell type, preferably selected based on the tissue to be repaired. Methods for enhancing the vascularization in vivo of these tissue graft constructs and for preparing these graft constructs are also provided.

    摘要翻译： 提供了用于修复患病或受损组织的组织移植物构建体。 组织移植物构建体包含选自肝基底膜和其提取物及其水解物的基质组合物，以及来自脊椎动物非粘膜下层源的加工胶原，加入的内皮细胞和至少一种另外预选的外源细胞群，其增强 在授权中启动类似船只的结构。 预选的细胞群可以是非角化角化角化细胞或角化细胞上皮细胞群或选自成纤维细胞，平滑肌细胞，骨骼肌细胞，心肌细胞，多潜能祖细胞， 周细胞，成骨细胞和任何其它合适的细胞类型，优选基于待修复的组织选择。 还提供了用于增强这些组织移植物构建体体内血管形成和用于制备这些移植构建体的方法。