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公开(公告)号：US20100159585A1
公开(公告)日：2010-06-24
申请号：US12440013
申请日：2007-09-07
申请人： Feng Tian , Thea Norman , Stephanie Chu
发明人： Feng Tian , Thea Norman , Stephanie Chu
摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in vertebrate cells. The components include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNA's/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in vertebrate cells are also provided.
摘要翻译： 本发明提供用于生产翻译成分的组合物和方法，其扩展脊椎动物细胞中遗传编码氨基酸的数目。 组分包括正交tRNA，正交氨酰-tRNA合成酶，tRNA /合成酶和非天然氨基酸的正交对。 还提供了在脊椎动物细胞中产生具有非天然氨基酸的蛋白质的蛋白质和方法。
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公开(公告)号：US07632823B2
公开(公告)日：2009-12-15
申请号：US11293629
申请日：2005-12-01
申请人： Feng Tian
发明人： Feng Tian
摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.
摘要翻译： 提供了包括正交tRNA，正交氨酰-tRNA合成酶和正交对tRNA /合成酶的蛋白质生物合成机理组分的组合物和方法。 还提供用于鉴定这些正交对的方法以及使用这些正交对产生蛋白质的方法。
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43.发明申请Accelerants for the Modification of Non-Natural Amino Acids and Non-Natural Amino Acid Polypeptides 审中-公开非天然氨基酸和非天然氨基酸多肽的改性促进剂公开(公告)号：US20090137736A1
公开(公告)日：2009-05-28
申请号：US12092265
申请日：2006-11-08
申请人： Feng Tian , Zhenwei Miao
发明人： Feng Tian , Zhenwei Miao
IPC分类号： C07K1/107
CPC分类号： C07K14/61 , C07K1/1077
摘要： Disclosed herein are accelerants for the formation of oxime-containing compounds from the reaction of a carbonyl-containing compound and a hydroxylamine-containing compound. The oxime-containing compound, the carbonyl-containing compound and the hydroxylamine-containing compound can each be a non-natural amino acid or a non-natural amino acid polypeptide. Also disclosed is the use of such accelerants to form oxime-containing compounds, the resulting oxime-containing compounds, and reaction mixtures containing such accelerants.
摘要翻译： 本文公开了用于由含羰基化合物和含羟胺化合物的反应形成含肟化合物的促进剂。 含肟化合物，含羰基化合物和含羟胺化合物各自可以是非天然氨基酸或非天然氨基酸多肽。 还公开了使用这种促进剂来形成含肟化合物，所得到的含肟化合物和含有这种促进剂的反应混合物。
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公开(公告)号：US20090018029A1
公开(公告)日：2009-01-15
申请号：US12092791
申请日：2006-11-16
CPC分类号： G01N33/6812
摘要： Disclosed herein are methods of detecting non-natural amino acids and polypeptides that include at least one non-natural amino acid. The non-natural amino acids, by themselves or as a part of a polypeptide, can include a wide range of functionalities, including but not limited to oxime, carbonyl, and/or hydroxylamine groups. Also disclosed herein are non-natural amino acid polypeptides that are further modified post-translationally, and methods for detecting such polypeptides.
摘要翻译： 本文公开了检测包括至少一种非天然氨基酸的非天然氨基酸和多肽的方法。 非天然氨基酸本身或作为多肽的一部分可以包括宽范围的官能团，包括但不限于肟，羰基和/或羟胺基团。 本文还公开了在翻译后进一步修饰的非天然氨基酸多肽，以及用于检测这些多肽的方法。
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公开(公告)号：US20080179948A1
公开(公告)日：2008-07-31
申请号：US12024734
申请日：2008-02-01
CPC分类号： H03F3/20 , H01J37/32082 , H01J37/32183 , H03F1/565 , H03F3/195 , H03F2200/321 , H03F2200/387 , H03F2200/391 , H03F2200/393 , H03F2200/451 , H03H7/40 , Y10T307/305
摘要： A system and method are provided for delivering power to a dynamic load. The system includes a power supply providing DC power having a substantially constant power open loop response, a power amplifier for converting the DC power to RF power, a sensor for measuring voltage, current and phase angle between voltage and current vectors associated with the RF power, an electrically controllable impedance matching system to modify the impedance of the power amplifier to at least a substantially matched impedance of a dynamic load, and a controller for controlling the electrically controllable impedance matching system. The system further includes a sensor calibration measuring module for determining power delivered by the power amplifier, an electronic matching system calibration module for determining power delivered to a dynamic load, and a power dissipation module for calculating power dissipated in the electrically controllable impedance matching system.
摘要翻译： 提供了一种将功率传递给动态负载的系统和方法。 该系统包括提供具有基本上恒定的功率开环响应的DC电力的电源，用于将DC功率转换为RF功率的功率放大器，用于测量与RF功率相关联的电压和电流矢量之间的电压，电流和相位角的传感器 ，电可控阻抗匹配系统，用于将功率放大器的阻抗修改为动态负载的至少基本匹配的阻抗;以及用于控制电可控阻抗匹配系统的控制器。 该系统还包括用于确定由功率放大器输出的功率的传感器校准测量模块，用于确定输送到动态负载的功率的电子匹配系统校准模块，以及用于计算在电气可控阻抗匹配系统中消耗的功率的功率耗散模块。
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46.发明申请Accelerants for the modification of non-natural amino acids and non-natural amino acid polypeptides 审中-公开用于修饰非天然氨基酸和非天然氨基酸多肽的促进剂公开(公告)号：US20080139794A1
公开(公告)日：2008-06-12
申请号：US11875741
申请日：2007-10-19
申请人： Feng Tian , Zhenwei Miao
发明人： Feng Tian , Zhenwei Miao
IPC分类号： C07K2/00
CPC分类号： C07K14/61 , C07K1/1077
摘要： Disclosed herein are accelerants for the formation of oxime-containing compounds from the reaction of a carbonyl-containing compound and a hydroxylamine-containing compound. The oxime-containing compound, the carbonyl-containing compound and the hydroxylamine-containing compound can each be a non-natural amino acid or a non-natural amino acid polypeptide. Also disclosed is the use of such accelerants to form oxime-containing compounds, the resulting oxime-containing compounds, and reaction mixtures containing such accelerants.
摘要翻译： 本文公开了用于由含羰基化合物和含羟胺化合物的反应形成含肟化合物的促进剂。 含肟化合物，含羰基化合物和含羟胺化合物各自可以是非天然氨基酸或非天然氨基酸多肽。 还公开了使用这种促进剂来形成含肟化合物，所得到的含肟化合物和含有这种促进剂的反应混合物。
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公开(公告)号：US20180142235A1
公开(公告)日：2018-05-24
申请号：US15356191
申请日：2016-11-18
申请人： Jianguang Zhang , Yang Gao , Yanbin Shi , Di Chen , Feng Tian
发明人： Jianguang Zhang , Yang Gao , Yanbin Shi , Di Chen , Feng Tian
IPC分类号： C12N15/10
CPC分类号： C12N15/1093 , C12Q1/68 , C12Q1/6806 , C12Q2521/501 , C12Q2525/191 , C12Q2531/113 , C12Q2535/122
摘要： The disclosure relates a method for constructing a plasma Deoxyribonucleic acid (DNA) sequencing library. The method includes: extracting a plasma DNA; making the plasma DNA ligate to a sequencing linker, and purifying a ligation product; performing Polymerase Chain Reaction (PCR) amplification for the purified ligation product, purifying the PCR amplification product, and obtaining the plasma DNA sequencing library, wherein, the method does not include the step of performing 5′-terminus phosphorylation for the plasma DNA. The kit provided by the disclosure includes: a reagent which ligates a plasma DNA to a sequencing linker, including the sequencing linker, a ligase and a ligation buffer; and reagents and instruments for purifying the ligation product; a reagent which performs PCR amplification for a purified ligation product, and reagents and instruments for purifying the PCR amplification product; wherein, the kit does not include the reagent which performs 5′-terminus phosphorylation for the plasma DNA.
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公开(公告)号：US09488660B2
公开(公告)日：2016-11-08
申请号：US12902010
申请日：2010-10-11
CPC分类号： G01N33/6812
摘要： Disclosed herein are methods of detecting non-natural amino acids and polypeptides that include at least one non-natural amino acid. The non-natural amino acids, by themselves or as a part of a polypeptide, can include a wide range of functionalities, including but not limited to oxime, carbonyl, and/or hydroxylamine groups. Also disclosed herein are non-natural amino acid polypeptides that are further modified post-translationally, and methods for detecting such polypeptides.
摘要翻译： 本文公开了检测包括至少一种非天然氨基酸的非天然氨基酸和多肽的方法。 非天然氨基酸本身或作为多肽的一部分可以包括宽范围的官能团，包括但不限于肟，羰基和/或羟胺基团。 本文还公开了在翻译后进一步修饰的非天然氨基酸多肽，以及用于检测这些多肽的方法。
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公开(公告)号：US09389221B2
公开(公告)日：2016-07-12
申请号：US14679024
申请日：2015-04-05
申请人： Feng Tian , Daniel Liang Zou , Lingyun Ji
发明人： Feng Tian , Daniel Liang Zou , Lingyun Ji
CPC分类号： G01N33/5304 , B01L1/00 , B01L3/508 , B01L3/52 , B01L2300/02 , B01L2300/0672 , B01L2300/0841 , G01N1/38 , G01N33/5302 , G01N2035/00524
摘要： The present disclosure provides an automated bioprocessing device, which may comprises a processing machine, one or more removable washing cartridges. The removable washing cartridge may comprise a shell, a sample holder, a washing basin, a waste container, a reagent container, a rotation mechanism, and a poking mechanism. The processing machine may comprise a motor, which may provide power to the inserted removable washing cartridge.
摘要翻译： 本公开提供了一种自动生物处理装置，其可以包括处理机器，一个或多个可移除的洗涤筒。 可移除的清洗盒可以包括壳体，样本保持器，洗涤盆，废物容器，试剂容器，旋转机构和戳戳机构。 加工机器可以包括电机，其可以向插入的可移除的清洗盒提供电力。
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50.发明授权公开(公告)号：US09302912B2
公开(公告)日：2016-04-05
申请号：US13432415
申请日：2012-03-28
申请人： Ken Tran , Feng Tian , Xing Chen , Franklin Lee
发明人： Ken Tran , Feng Tian , Xing Chen , Franklin Lee
CPC分类号： H01J37/248 , C01B13/115 , C01B2201/90
摘要： Improvements in the supply of high-frequency electrical power to ozone-producing cells can be accomplished using the systems and techniques described herein. Application of a DC-DC converter operating at a switching frequency substantially greater than a load frequency, supports generation of a high-voltage AC for powering such cells, while allowing for reductions in component size and reductions in a quality factor of a load tuning circuit. Controllable power inverters used in obtaining one or more of the switching and load frequencies can be controlled using feedback techniques to provide stable, high-quality power to ozone-producing cells under variations in one or more of externally supplied power and load conditions. An inrush protection circuit can also be provided to selectively introduce a current-limiting resistance until an input DC bus has been sufficiently initialized as determined by measurements obtained from the DC bus. The current limiting resistance can be a positive-temperature coefficient thermistor.
摘要翻译： 使用本文所述的系统和技术可以实现向产生臭氧的电池供应高频电力的改进。 以明显大于负载频率的开关频率工作的DC-DC转换器的应用支持产生用于为这样的单元供电的高压AC，同时允许减小组件尺寸并降低负载调整电路的品质因数 。 可以使用反馈技术来控制用于获得开关和负载频率中的一个或多个的可控功率逆变器，以在一个或多个外部供电和负载条件的变化下向产生臭氧的电池提供稳定的高质量功率。 还可以提供浪涌保护电路以选择性地引入限流电阻，直到输入DC总线已被充分初始化为由从DC总线获得的测量结果所确定。 限流电阻可以是正温系数热敏电阻。
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