Thermostable flap endonuclease derived from hyperthermophile bacterium belonging to the genus pyrococcus
    41.
    发明授权
    Thermostable flap endonuclease derived from hyperthermophile bacterium belonging to the genus pyrococcus 失效
    衍生自属于高球菌属的嗜热菌细菌的热稳定性瓣内切酶

    公开(公告)号:US06255081B1

    公开(公告)日:2001-07-03

    申请号:US09175973

    申请日:1998-10-21

    IPC分类号: C12P1934

    CPC分类号: C12N9/22

    摘要: The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.

    摘要翻译: 本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码(a)由SEQ ID NO:2所示的氨基酸序列组成的蛋白质的DNA,或(b)具有瓣内切核酸酶活性的蛋白质 由氨基酸序列(a),一个或多个氨基酸缺失,取代或添加的氨基酸序列组成。 根据本发明,提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。 此外,该酶是热稳定的,因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术

    Thermostable flap endonuclease derived from a hyperthermophile bacterium belonging to the genus pyrococcus
    42.
    发明授权
    Thermostable flap endonuclease derived from a hyperthermophile bacterium belonging to the genus pyrococcus 失效
    衍生自属于高球菌属的嗜热菌细菌的热稳定性瓣内切酶

    公开(公告)号:US06251649B1

    公开(公告)日:2001-06-26

    申请号:US09146319

    申请日:1998-09-03

    IPC分类号: C12N922

    CPC分类号: C07K14/195 C12N9/22

    摘要: The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.

    摘要翻译: 本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码(a)由SEQ ID NO:2所示的氨基酸序列组成的蛋白质的DNA,或(b)具有瓣内切核酸酶活性的蛋白质 由氨基酸序列(a),一个或多个氨基酸缺失,取代或添加的氨基酸序列组成。 根据本发明,提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。 此外,该酶是热稳定的,因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术

    Molecular element, manufacturing method thereof, integrated circuit device, manufacturing method thereof, three-dimensional integrated circuit device, and manufacturing method thereof
    43.
    发明授权
    Molecular element, manufacturing method thereof, integrated circuit device, manufacturing method thereof, three-dimensional integrated circuit device, and manufacturing method thereof 失效
    分子元件及其制造方法,集成电路器件,其制造方法,三维集成电路器件及其制造方法

    公开(公告)号:US08482000B2

    公开(公告)日:2013-07-09

    申请号:US13119674

    申请日:2009-09-01

    申请人: Eriko Matsui

    发明人: Eriko Matsui

    IPC分类号: H01L27/28

    摘要: In one example embodiment, a molecular element is configured by bridging a gap between a source electrode and a drain electrode by a functional molecule. The functional molecule arises from covalent linkage of a side chain composed of a pendant molecule that has dielectric constant anisotropy and/or dipole moments and in which orientation change occurs due to an electric field to a main chain composed of a conjugated molecule in which structural change occurs due to the orientation change of the pendant molecule and an electrical characteristic changes. The molecular element is made to work as a diode, a transistor, or a memory by an electric field applied to the pendant molecule of the functional molecule by gate electrodes.

    摘要翻译: 在一个示例性实施例中,分子元件通过用功能分子桥接源电极和漏电极之间的间隙来配置。 功能分子由具有介电常数各向异性和/或偶极矩的侧分子构成的侧链的共价连接引起,并且由于由共轭分子构成的主链的电场而发生取向变化,其中结构变化 由于侧分子的取向变化而发生电特性变化。 通过施加到功能分子的侧分子的电场通过栅电极使分子元件作为二极管,晶体管或存储器工作。

    DATA PROCESSING APPARATUS, DATA PROCESSING METHOD, IMAGE PROCESSING APPARATUS AND METHOD, AND PROGRAM
    44.
    发明申请
    DATA PROCESSING APPARATUS, DATA PROCESSING METHOD, IMAGE PROCESSING APPARATUS AND METHOD, AND PROGRAM 有权
    数据处理装置,数据处理方法,图像处理装置和方法及程序

    公开(公告)号:US20130070971A1

    公开(公告)日:2013-03-21

    申请号:US13637233

    申请日:2011-02-28

    IPC分类号: G06K9/46

    摘要: The present disclosure relates to a data processing apparatus and a data processing method capable of generating evaluation index data for performing accurate and detailed evaluation of cultured cardiomyocytes.A motion detecting unit divides frame image data obtained by photographing the cultured cardiomyocytes for a predetermined time into blocks and obtains motion detection data in units of blocks per each frame period. A feature amount calculating unit calculates a feature amount for each block at the same position in a frame image using the motion detection data. A classification processing unit classifies each of the blocks into any one of a plurality of classification categories using the calculated feature amount. On the basis of the classification result, evaluation index data made of individual classification result data that represent correspondences between the blocks and the classification categories is generated.

    摘要翻译: 本公开涉及能够产生用于对培养的心肌细胞进行准确和详细评估的评价指标数据的数据处理装置和数据处理方法。 运动检测单元将通过拍摄培养的心肌细胞获得的帧图像数据划分成块,并以每帧周期的块为单位获得运动检测数据。 特征量计算单元使用运动检测数据来计算帧图像中的相同位置处的每个块的特征量。 分类处理单元使用所计算的特征量将每个块分类为多个分类类别中的任一个。 基于分类结果,生成表示块与分类类别之间的对应关系的个体分类结果数据的评价指标数据。

    CELL CULTURING DEVICE USING ELECTRICAL RESPONSIVENESS FUNCTIONAL MATERIAL, CELL CULTURING SYSTEM INCLUDING THE SAME, AND CELL CULTURING METHOD
    45.
    发明申请
    CELL CULTURING DEVICE USING ELECTRICAL RESPONSIVENESS FUNCTIONAL MATERIAL, CELL CULTURING SYSTEM INCLUDING THE SAME, AND CELL CULTURING METHOD 审中-公开
    使用电子响应功能材料的细胞培养装置,包括其的细胞培养系统和细胞培养方法

    公开(公告)号:US20130034899A1

    公开(公告)日:2013-02-07

    申请号:US13552335

    申请日:2012-07-18

    IPC分类号: C12M3/00 C12N5/07 C12N5/04

    CPC分类号: C12M23/20 C12M25/04 C12M41/32

    摘要: A cell culturing device includes: an electrode on a surface of which a hydrophilicity/hydrophobicity converting material adapted to be electrically changed between hydrophilicity and hydrophobicity is provided. In this case, the electrode is disposed within a region in which a cell to be cultured is adapted to be accommodated. With the cell culturing device, the application of the suitable voltage to the electrode changes the hydrophilicity/the hydrophobicity of the hydrophilicity/hydrophobicity converting material. Thus, the feed material adsorbed to the hydrophilicity/hydrophobicity converting material is desorbed, thereby making it possible to feed the feed material to the cell.

    摘要翻译: 细胞培养装置包括:在其表面上设置适于在亲水性和疏水性之间电性变化的亲水性/疏水性转换材料的电极。 在这种情况下,电极设置在容纳细胞的区域内。 利用细胞培养装置,向电极施加适当的电压改变了亲水/疏水性转换材料的亲水性/疏水性。 因此,吸附到亲水/疏水性转换材料上的原料被解吸,从而可以将原料进料到电池中。

    RECORDING APPARATUS AND RECORDING METHOD THEREOF
    46.
    发明申请
    RECORDING APPARATUS AND RECORDING METHOD THEREOF 有权
    记录装置及其记录方法

    公开(公告)号:US20110234828A1

    公开(公告)日:2011-09-29

    申请号:US13052286

    申请日:2011-03-21

    IPC分类号: H04N5/225

    摘要: Disclosed is a recording apparatus including: a movement cycle determining section which receives moving image data including image contents of an object which performs a movement in which a movement state and a non-movement state are alternately and periodically repeated, and determines an operating period which corresponds to the movement state and a static period which corresponds to the non-movement state; and a recording control section which records the number of frame image data, per unit time, for forming the moving image data in the static period to be smaller than in the operating period, when the moving image data is recorded.

    摘要翻译: 公开了一种记录装置,包括:运动周期确定部,其接收运动图像数据,运动图像数据包括执行运动状态和非运动状态交替周期性重复的运动的对象的图像内容,并且确定运动周期 对应于运动状态和对应于非运动状态的静态周期; 以及记录控制部分,当记录运动图像数据时,将用于在静态周期中形成运动图像数据的每单位时间的帧图像数据的数量记录为小于操作周期中的运动图像数据。

    Flap endonuclease mutants
    47.
    发明授权
    Flap endonuclease mutants 失效
    瓣内切核酸突变体

    公开(公告)号:US07846704B2

    公开(公告)日:2010-12-07

    申请号:US11658096

    申请日:2005-01-21

    CPC分类号: C12N9/22

    摘要: A mutation is introduced into the substrate-binding site of flap endonuclease to prepare a mutant with modified substrate specificity. Using the mutant as a reagent for the analysis of genetic polymorphism, the analysis of genetic polymorphism can be performed more accurately, easily and sensitively as compared with conventional methods.

    摘要翻译: 将突变引入皮瓣内切核酸酶的底物结合位点以制备具有修饰的底物特异性的突变体。 使用突变体作为遗传多态性分析的试剂,与常规方法相比,遗传多态性的分析可以更准确,更容易和灵敏地进行。

    Functional molecular element, method for producing functional molecular element, and functional molecular device
    48.
    发明授权
    Functional molecular element, method for producing functional molecular element, and functional molecular device 失效
    功能分子元素,功能分子元件的制备方法和功能分子装置

    公开(公告)号:US07629606B2

    公开(公告)日:2009-12-08

    申请号:US11422485

    申请日:2006-06-06

    申请人: Eriko Matsui

    发明人: Eriko Matsui

    IPC分类号: H01L51/30 C07D487/22

    摘要: A functional molecular element with a reduced contact resistance between the constituting molecule and the electrode, a method for production thereof, and a functional molecular device are provided herein. The method consists of the following steps. A closely adhering molecular monolayer is formed on the surface of the electrodes from a π-electron conjugated molecule (one species of linear tetrapyrrole) composed of the porphyrin-like nearly discoid skeleton and the flexible side chains (alkyl chains). A π-electron conjugated molecule of the same species as the one or different species from the one is piled on the monolayer by π-π stacking to form the array structure. As the result of the foregoing steps, the π-electron conjugated molecule constituting the first molecular layer of the array structure takes on such a configuration that the flexible side chains adsorb to the surface of the electrode and the discoid skeleton orients parallel to and adheres to the surface of the electrode. The second and subsequent molecular layers constituting the array structure have their piling direction controlled by the π-π mutual action.

    摘要翻译: 本文提供了构成分子与电极之间的接触电阻降低的功能性分子元件,其制造方法和功能性分子器件。 该方法由以下步骤组成。 由卟啉样的近似圆盘状骨架和柔性侧链(烷基链)构成的π电子共轭分子(一种线性四吡咯)形成电极表面上紧密分散的单层。 通过pi-pi堆叠将与一种或不同种类相同物种的π电子共轭分子堆叠在单层上以形成阵列结构。 作为上述步骤的结果,构成阵列结构的第一分子层的π-电子共轭分子具有这样的构造:柔性侧链吸附到电极的表面并且盘状骨架定向平行并粘附到 电极的表面。 构成阵列结构的第二和随后的分子层的打桩方向由pi-pi相互作用控制。

    Display unit and driving method therefor
    50.
    发明授权
    Display unit and driving method therefor 失效
    显示单元及其驱动方法

    公开(公告)号:US06885489B2

    公开(公告)日:2005-04-26

    申请号:US10473288

    申请日:2002-03-29

    摘要: The invention provides an electrochromic display unit with high-quality display. The display unit comprises a transparent electrode (1), a display layer (2) which is formed on the transparent electrode (1) and which changes a color according to the amount of accumulated electrical charges, and an ion conductive layer (3) formed on the display layer (2). A plurality of picture electrodes (4) are formed on the ion conductive layer (3) on the side opposite to the display layer (2). The picture electrodes (4) are driven independently by, for example, a corresponding thin film transistors (6). In driving, by applying a drive current having given amount of electrical charges and then applying a certain amount of an inverted current, a certain amount of coloration is deducted, and extra coloration of the display layer (2) is eliminated.

    摘要翻译: 本发明提供具有高质量显示器的电致变色显示单元。 显示单元包括透明电极(1),形成在透明电极(1)上的显示层(2),并且根据累积电荷的量改变颜色,并形成离子传导层(3) 在显示层(2)上。 在与显示层(2)相反的一侧的离子传导层(3)上形成有多个图像电极(4)。 图像电极(4)由例如相应的薄膜晶体管(6)独立驱动。 在驱动中,通过施加具有给定量的电荷的驱动电流,然后施加一定量的反相电流,减去一定量的着色,并且消除显示层(2)的额外着色。