摘要:
The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.
摘要翻译:本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码(a)由SEQ ID NO:2所示的氨基酸序列组成的蛋白质的DNA,或(b)具有瓣内切核酸酶活性的蛋白质 由氨基酸序列(a),一个或多个氨基酸缺失,取代或添加的氨基酸序列组成。 根据本发明,提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。 此外,该酶是热稳定的,因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术
摘要:
The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75° C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.
摘要翻译:本发明涉及最适温度为75℃或更高的热稳定翼片内切核酸酶和编码(a)由SEQ ID NO:2所示的氨基酸序列组成的蛋白质的DNA,或(b)具有瓣内切核酸酶活性的蛋白质 由氨基酸序列(a),一个或多个氨基酸缺失,取代或添加的氨基酸序列组成。 根据本发明,提供了其最佳反应温度为75℃或更高的热稳定翼片内切核酸酶。 此外,该酶是热稳定的,因此可以开发通过将酶反应与PCR偶联来高效地进行人工同源重组或遗传改组的新技术
摘要:
In one example embodiment, a molecular element is configured by bridging a gap between a source electrode and a drain electrode by a functional molecule. The functional molecule arises from covalent linkage of a side chain composed of a pendant molecule that has dielectric constant anisotropy and/or dipole moments and in which orientation change occurs due to an electric field to a main chain composed of a conjugated molecule in which structural change occurs due to the orientation change of the pendant molecule and an electrical characteristic changes. The molecular element is made to work as a diode, a transistor, or a memory by an electric field applied to the pendant molecule of the functional molecule by gate electrodes.
摘要:
The present disclosure relates to a data processing apparatus and a data processing method capable of generating evaluation index data for performing accurate and detailed evaluation of cultured cardiomyocytes.A motion detecting unit divides frame image data obtained by photographing the cultured cardiomyocytes for a predetermined time into blocks and obtains motion detection data in units of blocks per each frame period. A feature amount calculating unit calculates a feature amount for each block at the same position in a frame image using the motion detection data. A classification processing unit classifies each of the blocks into any one of a plurality of classification categories using the calculated feature amount. On the basis of the classification result, evaluation index data made of individual classification result data that represent correspondences between the blocks and the classification categories is generated.
摘要:
A cell culturing device includes: an electrode on a surface of which a hydrophilicity/hydrophobicity converting material adapted to be electrically changed between hydrophilicity and hydrophobicity is provided. In this case, the electrode is disposed within a region in which a cell to be cultured is adapted to be accommodated. With the cell culturing device, the application of the suitable voltage to the electrode changes the hydrophilicity/the hydrophobicity of the hydrophilicity/hydrophobicity converting material. Thus, the feed material adsorbed to the hydrophilicity/hydrophobicity converting material is desorbed, thereby making it possible to feed the feed material to the cell.
摘要:
Disclosed is a recording apparatus including: a movement cycle determining section which receives moving image data including image contents of an object which performs a movement in which a movement state and a non-movement state are alternately and periodically repeated, and determines an operating period which corresponds to the movement state and a static period which corresponds to the non-movement state; and a recording control section which records the number of frame image data, per unit time, for forming the moving image data in the static period to be smaller than in the operating period, when the moving image data is recorded.
摘要:
A mutation is introduced into the substrate-binding site of flap endonuclease to prepare a mutant with modified substrate specificity. Using the mutant as a reagent for the analysis of genetic polymorphism, the analysis of genetic polymorphism can be performed more accurately, easily and sensitively as compared with conventional methods.
摘要:
A functional molecular element with a reduced contact resistance between the constituting molecule and the electrode, a method for production thereof, and a functional molecular device are provided herein. The method consists of the following steps. A closely adhering molecular monolayer is formed on the surface of the electrodes from a π-electron conjugated molecule (one species of linear tetrapyrrole) composed of the porphyrin-like nearly discoid skeleton and the flexible side chains (alkyl chains). A π-electron conjugated molecule of the same species as the one or different species from the one is piled on the monolayer by π-π stacking to form the array structure. As the result of the foregoing steps, the π-electron conjugated molecule constituting the first molecular layer of the array structure takes on such a configuration that the flexible side chains adsorb to the surface of the electrode and the discoid skeleton orients parallel to and adheres to the surface of the electrode. The second and subsequent molecular layers constituting the array structure have their piling direction controlled by the π-π mutual action.
摘要:
A pattern forming method is provided. The pattern forming method includes a first step of forming a resist pattern including a lactone group-containing skeleton above an etched layer provided on a substrate; a second step of performing plasma processing using a hydrogen-containing gas to lower a glass transition temperature or a softening point of the resist pattern; and a third step of transferring the resist pattern after the plasma processing to the etched layer by etching, and forming the pattern of the etched layer.
摘要:
The invention provides an electrochromic display unit with high-quality display. The display unit comprises a transparent electrode (1), a display layer (2) which is formed on the transparent electrode (1) and which changes a color according to the amount of accumulated electrical charges, and an ion conductive layer (3) formed on the display layer (2). A plurality of picture electrodes (4) are formed on the ion conductive layer (3) on the side opposite to the display layer (2). The picture electrodes (4) are driven independently by, for example, a corresponding thin film transistors (6). In driving, by applying a drive current having given amount of electrical charges and then applying a certain amount of an inverted current, a certain amount of coloration is deducted, and extra coloration of the display layer (2) is eliminated.