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    Arginine Analogs, and Methods for Their Synthesis and Use
    41.
    发明申请
    Arginine Analogs, and Methods for Their Synthesis and Use 有权
    精氨酸类似物及其合成和使用方法

    公开(公告)号：US20080293920A1

    公开(公告)日：2008-11-27

    申请号：US11795173

    申请日：2006-01-19

    申请人： Kenneth F. Buechler ,  Mariusz G. Banaszczyk ,  Joseph Barry Noar ,  Violeta Dinauer

    发明人： Kenneth F. Buechler ,  Mariusz G. Banaszczyk ,  Joseph Barry Noar ,  Violeta Dinauer

    IPC分类号： C07C229/04 ,  C07K14/765 ,  C07K14/00 ,  C07H21/00 ,  C07D207/40

    CPC分类号： C07C327/32 ,  C07C323/59 ,  C07C323/60 ,  C07D333/36

    摘要： The present invention relates to novel arginine analogs, and methods for their synthesis and use. Such analogs are designed to provide a protected or free thiol (—SH) group, thereby providing a convenient linkage chemistry for coupling to a suitable group on a target such as a protein, polypeptide, detectable label or solid phase, and at a site distal to the guanidino group. Arginine analog conjugates are useful for generating antibodies that can bind specifically with dimethylarginine, which can be detected using such antibodies in immunoassays.

    摘要翻译： 本发明涉及新颖的精氨酸类似物及其合成和使用的方法。 这样的类似物被设计成提供受保护或游离的硫醇（-SH）基团，从而提供方便的连接化学物质，用于偶联到靶上的合适的基团，例如蛋白质，多肽，可检测的标记或固相，并且在远端位点 到guanidino组。 精氨酸类似物缀合物可用于产生可与二甲基精氨酸特异性结合的抗体，其可以在免疫测定中使用这样的抗体进行检测。

    Polypeptides related to natriuretic peptides and methods of their identification and use
    42.
    发明授权
    Polypeptides related to natriuretic peptides and methods of their identification and use 失效
    与利尿钠肽相关的多肽及其鉴定和使用方法

    公开(公告)号：US07341838B2

    公开(公告)日：2008-03-11

    申请号：US10827919

    申请日：2004-04-19

    申请人： Kenneth F. Buechler ,  Eric Thomas Fung ,  Tai-Tung Yip

    发明人： Kenneth F. Buechler ,  Eric Thomas Fung ,  Tai-Tung Yip

    IPC分类号： G01N33/53

    CPC分类号： G01N33/74 ,  G01N2500/04

    摘要： The present invention relates to the identification and use of polypeptides that bind to antibodies directed to a desired polypeptide of interest. Using natriuretic peptides and their precursors, and in particular BNP, as an example, the present invention describes a number of natriuretic peptides fragments produced in biological samples, most preferably blood-derived samples, that bind to antibodies directed to BNP. Because production of such fragments is an ongoing process that may be a function of, inter alia, the elapsed time between onset of an event triggering natriuretic peptide release into the tissues and the time the sample is obtained or analyzed; the elapsed time between sample acquisition and the time the sample is analyzed; the type of tissue sample at issue; the storage conditions; the quantity of proteolytic enzymes present; etc., such fragments may be used when both designing an assay for one or more natriuretic peptides, and when performing such an assay, in order to provide an accurate prognostic or diagnostic result.

    摘要翻译： 本发明涉及与靶向所需感兴趣的多肽的抗体结合的多肽的鉴定和使用。 使用利尿钠肽及其前体，特别是BNP作为实例，本发明描述了与生物样品中产生的许多利钠肽片段，最优选血液衍生的样品，其结合针对BNP的抗体。 因为这样的片段的产生是一个持续的过程，其可以是尤其是引发利钠肽释放到组织中的事件发生之间经过的时间与获得或分析样品的时间的函数; 样品采集与样品分析时间之间的经过时间; 有关组织样本的类型; 储存条件; 存在蛋白水解酶的数量; 当设计用于一种或多种利钠肽的测定法和进行这样的测定时，可以使用这样的片段，以便提供准确的预后或诊断结果。

    Methods for the assay of troponin I and T and complexes of troponin I and T and selection of antibodies for use in immunoassays
    43.
    发明授权
    Methods for the assay of troponin I and T and complexes of troponin I and T and selection of antibodies for use in immunoassays 失效
    测定肌钙蛋白I和T的方法以及肌钙蛋白I和T的复合物以及免疫测定中使用的抗体选择

    公开(公告)号：US06991907B1

    公开(公告)日：2006-01-31

    申请号：US09687051

    申请日：2000-10-12

    申请人： Kenneth F. Buechler ,  Paul H. McPherson

    发明人： Kenneth F. Buechler ,  Paul H. McPherson

    IPC分类号： G01N33/53

    CPC分类号： G01N33/6887 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S436/811 ,  Y10S436/819

    摘要： Antibodies and methods are described for the detection and quantitation of cardiac specific troponin I in samples. Cardiac-specific troponin isoforms exist in various forms in the blood, including free and complexed forms. By selecting antibodies that are insensitive and/or sensitive to these various forms, the present invention can provide immunoassays that more accurately reflect the clinical state of an individual. These described antibodies and methods can be used for providing indicators of myocardial infarction and other cardiac pathologies.

    摘要翻译： 描述了用于检测和定量样品中心脏特异性肌钙蛋白I的抗体和方法。 心脏特异性肌钙蛋白异构体在血液中以各种形式存在，包括游离和复合形式。 通过选择对这些各种形式不敏感和/或敏感的抗体，本发明可以提供更准确地反映个体临床状态的免疫测定。 这些描述的抗体和方法可用于提供心肌梗塞和其他心脏病变的指标。

    Methods for the assay of troponin I and T and complexes of troponin I and T and selection of antibodies for use in immunoassays
    45.
    发明授权
    Methods for the assay of troponin I and T and complexes of troponin I and T and selection of antibodies for use in immunoassays 失效
    测定肌钙蛋白I和T的方法以及肌钙蛋白I和T的复合物以及免疫测定中使用的抗体选择

    公开(公告)号：US06939678B1

    公开(公告)日：2005-09-06

    申请号：US09349194

    申请日：1999-07-07

    申请人： Kenneth F. Buechler ,  Paul H. McPherson

    发明人： Kenneth F. Buechler ,  Paul H. McPherson

    IPC分类号： G01N33/53 ,  C12P21/08 ,  G01N33/68

    CPC分类号： G01N33/6887 ,  G01N2800/32 ,  Y10S435/96 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S436/811 ,  Y10S436/819

    摘要： Assay systems and specialized antibodies for the detection and quantitation of troponin I and troponin T in body fluids as an indicator of myocardial infarction. Since troponin I and T exist in various conformations in the blood, the ratios of the monomeric troponin I an T and the binary and ternary complexes, as well as which form of troponin present in the blood, may be related to the metabolic state of the heart. Disclosed is a system to determine the presence of a troponin form or a group of troponin forms in a sample of whole blood, serum or plasma.Disclosed is a stabilized composition of troponin; the stabilized composition can comprise a stabilized composition of troponin I, wherein the troponin I is oxidized, the troponin I can be unbound or the troponin I can be in a complex.Disclosed is a method for improving the recovery of troponin I or T from a surface used in immunoassays.Also disclosed are antibodies which recognize, unbound troponin forms, the forms of troponin in binary complexes, the ternary complex of troponin I, T and C, and the conformations of troponin I having intramolecularly oxidized and reduced cysteines.

    摘要翻译： 用于检测和定量体液中肌钙蛋白I和肌钙蛋白T的测定系统和专用抗体作为心肌梗死的指标。 由于肌钙蛋白I和T存在于血液中的各种构象中，单体肌钙蛋白I与T和二元和三元复合物的比例以及存在于血液中的肌钙蛋白形式可能与代谢状态有关 心。 公开了一种确定全血，血清或血浆样品中肌钙蛋白形式或一组肌钙蛋白形式存在的系统。 公开了稳定的肌钙蛋白的组合物; 稳定的组合物可以包含肌钙蛋白I的稳定化组合物，其中肌钙蛋白I被氧化，肌钙蛋白I可以是未结合的，或者肌钙蛋白I可以在复合物中。 公开了一种用于从免疫测定中使用的表面改善肌钙蛋白I或T的恢复的方法。 还公开了识别未结合的肌钙蛋白形式，二元复合物中的肌钙蛋白的形式，肌钙蛋白I，T和C的三元复合物以及具有分子内氧化和还原的半胱氨酸的肌钙蛋白I的构象的抗体。

    Diagnostic devices and apparatus for the controlled movement of reagents without membranes
    47.
    发明授权
    Diagnostic devices and apparatus for the controlled movement of reagents without membranes 失效
    诊断设备和装置，用于无膜的试剂受控运动

    公开(公告)号：US06767510B1

    公开(公告)日：2004-07-27

    申请号：US09805653

    申请日：2001-03-13

    申请人： Kenneth F. Buechler

    发明人： Kenneth F. Buechler

    IPC分类号： G01N3348

    CPC分类号： B01L3/50273 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0825 ,  B01L2300/089 ,  B01L2400/0406 ,  B01L2400/0688 ,  B01L2400/086 ,  B01L2400/088 ,  G01N33/558

    摘要： The assay devices, assay systems and device components of this invention comprise at least two opposing surfaces disposed a capillary distance apart, at least one of which is capable of immobilizing at least one target ligand or a conjugate in an amount related to the presence or amount of target ligand in the sample from a fluid sample in a zone for controlled fluid movement to, through or away the zone. The inventive device components may be incorporated into conventional assay devices with membranes or may be used in the inventive membrane-less devices herein described and claimed. These components include flow control elements, measurement elements, time gates, elements for the elimination of pipetting steps, and generally, elements for the controlled flow, timing, delivery, incubation, separation, washing and other steps of the assay process.

    摘要翻译： 本发明的测定装置，测定系统和装置组件包括至少两个相对的表面，其布置有毛细管距离，其中至少一个能够固定至少一种靶配体或缀合物，其量与存在或量相关 来自流体样品中的目标配体在用于受控流体运动的区域中，通过或离开区域。 本发明的器件组件可以并入具有膜的常规测定装置中，或者可以用于本文所述和要求保护的本发明的无膜装置中。 这些组件包括流量控制元件，测量元件，时间门，用于消除移液步骤的元件，以及通常用于受控流动，定时，递送，孵育，分离，洗涤和测定过程的其它步骤的元件。

    Media carrier for an assay device
    48.
    发明授权
    Media carrier for an assay device 有权
    用于测定装置的载体

    公开(公告)号：US06392894B1

    公开(公告)日：2002-05-21

    申请号：US09545699

    申请日：2000-04-06

    申请人： Kenneth F. Buechler ,  Joseph M Anderberg ,  Paul H. McPherson

    发明人： Kenneth F. Buechler ,  Joseph M Anderberg ,  Paul H. McPherson

    IPC分类号： H05K500

    CPC分类号： G01N21/645

    摘要： A fluorometer for sensing the fluorescence of a sample utilizes an optical energy source for exciting a sample to be tested and an optical energy detector for detecting the emitted energy from the excited sample. Drive electronics are used for positioning the sample with respect to the optical components allowing a plurality of sample regions to be tested. A processor is utilized to control the operation of the test in accordance with test instructions and for processing the emitted energy detected from the sample to determine test results. A ROM chip socket accepts a plurality of ROM chips, wherein each ROM chip stores test data sets for one or more test types to be performed. ROM chips can be swapped to allow the fluorometer to be configured and reconfigured to perform a plurality of different tests. A communications interface facilitates the sharing of test information between the fluorometer and external entities.

    摘要翻译： 用于感测样品荧光的荧光计利用用于激发待测试样品的光能源和用于检测来自激发样品的发射能量的光能检测器。 驱动电子器件用于相对于光学部件定位样品，允许测试多个样品区域。 使用处理器来根据测试指令来控制测试的操作，并且用于处理从样品检测到的发射能量以确定测试结果。 ROM芯片插座接受多个ROM芯片，其中每个ROM芯片存储要执行的一种或多种测试类型的测试数据集。 可以交换ROM芯片以允许荧光计被配置和重新配置以执行多个不同的测试。 通信接口有助于在荧光计和外部实体之间共享测试信息。

    Fluorescence energy transfer in particles
    49.
    发明授权
    Fluorescence energy transfer in particles 失效
    颗粒中的荧光能量转移

    公开(公告)号：US06238931B1

    公开(公告)日：2001-05-29

    申请号：US08274534

    申请日：1994-07-12

    申请人： Kenneth F. Buechler ,  Joseph Barry Noar ,  Lema Tadesse

    发明人： Kenneth F. Buechler ,  Joseph Barry Noar ,  Lema Tadesse

    IPC分类号： G01N33533

    CPC分类号： G01N33/587 ,  C09B47/08 ,  C09B67/0097 ,  G01N33/582 ,  Y10S436/80 ,  Y10S530/811 ,  Y10S530/812 ,  Y10S530/815 ,  Y10T428/2989 ,  Y10T428/2998

    摘要： Particles and methods for the detection or visualization of analytes using fluorescence energy transfer. Particles comprising an energy donor as a first component and a fluorescent dye as a second component positioned in said particles at an energy exchanging distance from one another, wherein the two components have a Stokes shift of greater than or equal to 50 nm, said particle having bound on its surface, a protein, polypeptide, nucleic acid, nucleotide or protein containing ligand analogue are disclosed and claimed.

    摘要翻译： 使用荧光能量转移检测或可视化分析物的粒子和方法。 包含作为第一成分的能量给体的粒子和以彼此间的能量交换距离定位在所述粒子中的第二成分的荧光染料，其中所述两种成分具有大于或等于50nm的斯托克斯位移，所述粒子具有 结合其表面，蛋白质，多肽，核酸，核苷酸或蛋白质的配体类似物被公开和要求保护。