公开(公告)号：US6069703A

公开(公告)日：2000-05-30

申请号：US86975

申请日：1998-05-28

申请人： Matthew J. Banet ,  Martin Fuchs ,  John A. Rogers ,  Keith A. Nelson ,  Timothy F. Crimmins ,  Alexei Maznev

发明人： Matthew J. Banet ,  Martin Fuchs ,  John A. Rogers ,  Keith A. Nelson ,  Timothy F. Crimmins ,  Alexei Maznev

IPC分类号： G01B11/06 ,  G01B17/02 ,  G01N3/02 ,  G01N21/00 ,  G01N29/00 ,  G01N29/24 ,  G01N29/04

CPC分类号： G01N29/2418 ,  G01B11/0666 ,  G01B17/025 ,  G01N2021/1725 ,  G01N2203/0222 ,  G01N2203/0282 ,  G01N2291/0231 ,  G01N2291/0237 ,  G01N2291/02854

摘要： An apparatus for measuring a property of a structure comprising at least one layer, the appratus including a light source that produces an optical pulse having a duration of less than 10 ps; a diffractive element that receives the optical pulse and diffracts it to generate at least two excitation pulses; an optical system that spatially and temporally overlaps at least two excitation pulses on or in the structure to form an excitation pattern, containing at least two light regions, that launches an acoustic wave having an out-of-plane component that propagates through the layer, reflects off a lower boundary of the layer, and returns to a surface of the structure to modulate a property of the structure; a light source that produces a probe pulse that diffracts off the modulated property to generate at least one signal pulse; a detector that receives at least one signal pulse and in response generates a light-induced electrical signal; and an analyzer that analyzes the light-induced electrical signal to measure the property of the structure.

摘要翻译： 一种用于测量包括至少一层的结构的性质的装置，所述装置包括产生具有小于10ps的持续时间的光脉冲的光源; 衍射元件，其接收光脉冲并衍射以产生至少两个激励脉冲; 在该结构上或其结构中空间和时间上重叠至少两个激励脉冲以形成激发图案的光学系统，其包含至少两个光区域，该至少两个光区域发射具有传播通过该层的平面外部分的声波， 反射层的下边界，并返回到结构的表面以调节结构的性质; 产生探测脉冲的光源，其衍射出调制特性以产生至少一个信号脉冲; 接收至少一个信号脉冲并作为响应的检测器产生光诱导的电信号; 以及分析器，其分析光感应电信号以测量结构的性质。

公开(公告)号：US5958202A

公开(公告)日：1999-09-28

申请号：US792933

申请日：1997-01-22

申请人： Fred E. Regnier ,  Xian-Wei Yao ,  Todd A. Taylor ,  Martin Fuchs ,  Dieter Schmalzing ,  Lance Koutny ,  Wassim Nashabeh

发明人： Fred E. Regnier ,  Xian-Wei Yao ,  Todd A. Taylor ,  Martin Fuchs ,  Dieter Schmalzing ,  Lance Koutny ,  Wassim Nashabeh

IPC分类号： G01N33/561 ,  G01N27/447 ,  G01N33/537 ,  G01N33/543 ,  G01N33/78 ,  G01N27/26 ,  C12Q1/68 ,  G01N33/53

CPC分类号： G01N27/44747 ,  G01N27/44726 ,  G01N33/537 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/78

摘要： Disclosed are methods and apparatus for performing highly sensitive enzyme-amplified assays using channel electrophoresis of an enzyme having a biorecognition moiety. Combining a binding reaction of an analyte and an enzyme having a biorecognition moiety, and an electrophoretic separation which uses enzyme-amplified detection methodology, assay methods and apparatus are provided that offer simplicity, quantitative sensitivity and rapid analysis. Also disclosed are highly sensitive catalytically-amplified assays using a capillary electrophoresis format in which the binding reaction prior to electrophoresis is heterogeneous or homogeneous, and direct or competitive. The methods and apparatus for conducting sensitive automated catalytically-amplified assays using electrophoresis may be conducted in a microscale format.

摘要翻译： 公开了使用具有生物识别部分的酶的通道电泳进行高度灵敏的酶扩增测定的方法和装置。 结合分析物和具有生物识别部分的酶的结合反应以及使用酶扩增检测方法的测定方法和装置，提供了简单性，定量灵敏度和快速分析的电泳分离。 还公开了使用毛细管电泳方式的高度敏感的催化扩增测定法，其中电泳前的结合反应是异质或均相的，直接的或竞争性的。 使用电泳进行敏感的自动化催化扩增测定的方法和装置可以以微型格式进行。

公开(公告)号：US5948231A

公开(公告)日：1999-09-07

申请号：US853031

申请日：1997-05-08

申请人： Martin Fuchs ,  Wassim A. Nashabeh ,  Dieter R. Schmalzing

发明人： Martin Fuchs ,  Wassim A. Nashabeh ,  Dieter R. Schmalzing

IPC分类号： G01N33/533 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/537 ,  G01N33/561 ,  G01N33/563 ,  G01N37/00 ,  G01N27/26

CPC分类号： G01N27/44726 ,  C12Q1/68 ,  G01N33/5375 ,  G01N33/561 ,  G01N2333/59

摘要： Compositions, methods, and apparatus for performing ultrafast binding assays by capillary electrophoresis or other electroseparation techniques are disclosed. In one embodiment, a first binding partner carries a detectable label and a second binding partner is modified to be highly charged. When used in combination with a sample containing an analyte with which both binding partners can interact and bind thereto, a three-membered complex is formed. The electrophoretic mobility difference between the unbound and complex-bound forms of labeled first binding partner is such that electroseparation and subsequent detection of an analyte can be accomplished. The compositions, methods, and apparatus disclosed herein also permit quantitative determination of the concentration of an analyte in a sample.

摘要翻译： 公开了通过毛细管电泳或其它电离分离技术进行超快速结合测定的组合物，方法和装置。 在一个实施方案中，第一结合伴侣携带可检测标记，第二结合伴侣被修饰为高度带电。 当与含有两个结合配偶体可以与其结合的分析物的样品组合使用时，形成三元复合物。 标记的第一结合配偶体的未结合和复合结合形式之间的电泳迁移率差异可以实现电分离和随后的分析物检测。 本文公开的组合物，方法和装置还允许定量测定样品中分析物的浓度。

公开(公告)号：US20140186832A1

公开(公告)日：2014-07-03

申请号：US14084026

申请日：2013-11-19

申请人： Martin Fuchs ,  Michelle Meltzer

发明人： Martin Fuchs ,  Michelle Meltzer

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N13/00 ,  C12Q1/04

摘要： The present invention features methods for selective lysis of endogenous cells in a biological sample. In preferred embodiments, the methods of the invention comprise contacting the biological sample with lysis solution, and subjecting the mixture to ultrasound, thereby selectively lysing the endogenous cells in the biological sample. The invention also features a lysis solution comprising Saponin and Proteinase.

摘要翻译： 本发明的特征在于在生物样品中选择性裂解内源性细胞的方法。 在优选的实施方案中，本发明的方法包括使生物样品与裂解溶液接触，并使混合物超声波，从而选择性地裂解生物样品中的内源细胞。 本发明还具有包含皂苷和蛋白酶的裂解溶液。

公开(公告)号：US08423294B2

公开(公告)日：2013-04-16

申请号：US10762207

申请日：2004-01-21

申请人： Mark Nadel ,  Eugene Y. Chan ,  Martin Fuchs ,  Rudolf Gilmanshin

发明人： Mark Nadel ,  Eugene Y. Chan ,  Martin Fuchs ,  Rudolf Gilmanshin

IPC分类号： G01N33/50

CPC分类号： B82Y5/00 ,  B82Y10/00 ,  C12Q1/6869 ,  C12Q2565/629 ,  C12Q2565/102 ,  C12Q2563/107

摘要： The invention provides methods and systems for improved spatial resolution of signal detection, particularly as applied to the analysis of polymers such as biological polymers. Some of the methods and systems comprise differentially tagging polymers in order to increase resolution. Some of the methods and systems comprise techniques for improving the precision of separation distance measurements, without necessarily requiring improvements in the known detection resolution of prior art systems.

摘要翻译： 本发明提供用于改善信号检测的空间分辨率的方法和系统，特别是应用于聚合物如生物聚合物的分析。 一些方法和系统包括差异化标记聚合物以提高分辨率。 一些方法和系统包括用于提高分离距离测量的精度的技术，而不一定需要改进现有技术系统的已知检测分辨率。

公开(公告)号：US07708884B2

公开(公告)日：2010-05-04

申请号：US10586222

申请日：2006-04-21

申请人： Leonhard Fuchs ,  Martin Fuchs

发明人： Leonhard Fuchs ,  Martin Fuchs

IPC分类号： C02F3/00

CPC分类号： C02F3/121 ,  C02F3/006 ,  C02F3/1221 ,  C02F3/26 ,  C02F11/02 ,  C02F2209/02 ,  C02F2209/04 ,  C02F2209/22 ,  C02F2209/44 ,  C02F2301/106 ,  C02F2303/02 ,  C02F2303/04 ,  Y02W10/15 ,  Y02W10/27

摘要： A process for the stabilization and disinfection of sludge wherein a) raw sludge having a dry matter content of from 3 to 7% by weight is fed continuously or quasi-continuously into a first stage, where it remains for an average retention time of three to ten days under aerobic-thermophilic conditions to obtain a partially stabilized sludge; and b) the partially stabilized sludge is fed into a second stage in which disinfection of the partially stabilized sludge is effected at temperatures of at least 50° C., wherein prior to discharging, there is no charging until the partially stabilized sludge has been disinfected.

摘要翻译： 一种用于污泥稳定化和消毒的方法，其中a）将干物质含量为3至7重量％的原始污泥连续或准连续地进料到第一阶段，其中保持平均停留时间为3至 在好氧 - 嗜热条件下10天，以获得部分稳定的污泥; 和b）将部分稳定的污泥进料到第二阶段，其中在至少50℃的温度下进行部分稳定的污泥的消毒，其中在排出之前，直到部分稳定的污泥已经被消毒 。

公开(公告)号：US20090273792A1

公开(公告)日：2009-11-05

申请号：US12419438

申请日：2009-04-07

申请人： Matthias Hullin ,  Martin Fuchs ,  Ivo Ihrke ,  Hendrik Lensch ,  Hans-Peter Seidel

发明人： Matthias Hullin ,  Martin Fuchs ,  Ivo Ihrke ,  Hendrik Lensch ,  Hans-Peter Seidel

IPC分类号： G01B11/24 ,  G01B11/14

CPC分类号： G01B11/2504 ,  G01B11/2518 ,  G06T7/521

摘要： A system (100) for scanning a three-dimensional object (150) comprises a device for illuminating a plane (110); an imaging sensor (120); and a transparent container (130) for immersing the object (150) in a participating medium (140).

摘要翻译： 用于扫描三维物体（150）的系统（100）包括用于照亮平面（110）的装置; 成像传感器（120）; 以及用于将物体（150）浸入参与介质（140）中的透明容器（130）。

公开(公告)号：US20070026419A1

公开(公告)日：2007-02-01

申请号：US11324108

申请日：2005-12-29

申请人： Martin Fuchs ,  Ying-Xin Wang ,  Yi-Shuian Huang

发明人： Martin Fuchs ,  Ying-Xin Wang ,  Yi-Shuian Huang

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/34

CPC分类号： G01N33/54366 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/086 ,  B82Y5/00 ,  B82Y10/00 ,  G01N2800/52

摘要： The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.

公开(公告)号：US06734982B2

公开(公告)日：2004-05-11

申请号：US09795015

申请日：2001-02-28

申请人： Matthew J. Banet ,  Martin Fuchs ,  John A. Rogers

发明人： Matthew J. Banet ,  Martin Fuchs ,  John A. Rogers

IPC分类号： G01B1106

CPC分类号： G01B11/0666

摘要： A method for measuring a structure that contains overlying and underlying films in a region where the overlying film's thickness rapidly decreases until the underlying film is exposed (e.g., an edge-exclusion structure). The method includes the steps of: (1) exciting acoustic modes in a first portion of the region with at least one excitation laser beam; (2) detecting the acoustic modes with a probe laser beam that is either reflected or diffracted to generate a signal beam; (3) analyzing the signal beam to determine a property of the structure (e.g., the thickness of the overlying layer) in the first portion of the region; (4) translating the structure or the excitation and probe laser beams; and (5) repeating the exciting, detecting, and analyzing steps to determine a property of the structure in a second portion of the region.

摘要翻译： 一种用于测量在覆盖膜的厚度迅速减小直到底层膜暴露的区域（例如，边缘排除结构）中的覆盖和下层膜的结构的测量方法。 该方法包括以下步骤：（1）利用至少一个激发激光束激发该区域的第一部分中的声模; （2）用反射或衍射的探针激光束检测声学模式以产生信号光束; （3）分析信号光束以确定该区域的第一部分中的结构的性质（例如，上覆层的厚度）; （4）平移结构或激发和探测激光束; 和（5）重复激励，检测和分析步骤以确定该区域的第二部分中的结构的属性。

公开(公告)号：US06188478B1

公开(公告)日：2001-02-13

申请号：US09176174

申请日：1998-10-21

申请人： Martin Fuchs ,  Michael A. Joffe ,  Matt Banet

发明人： Martin Fuchs ,  Michael A. Joffe ,  Matt Banet

IPC分类号： G01B1106

CPC分类号： G01B11/0666 ,  G01N21/636

摘要： The invention describes a method for measuring a property of a sample by: 1) irradiating a portion of the sample with an excitation pattern characterized by at least one spatial phase and spatial period; 2) diffracting a portion of a probe beam off a surface of the sample; 3) detecting the diffracted portion of the probe beam with an optical detector to generate a light-induced signal; 4) adjusting the spatial phase of the excitation pattern; 5) repeating the irradiating, diffracting and detecting steps to generate an additional light-induced signal; and 6) processing the light-induced signals to determine a property of the sample.

摘要翻译： 本发明描述了一种用于通过以下步骤测量样品的性质的方法：1）用以至少一个空间相位和空间周期为特征的激发图案照射样品的一部分; 2）将探针的一部分衍射离开样品的表面; 3）用光学检测器检测探测光束的衍射部分以产生光诱导信号; 4）调整激励模式的空间相位; 5）重复照射，衍射和检测步骤以产生附加的光诱导信号; 和6）处理光诱导信号以确定样品的性质。