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105 条记录 (耗时 0.039 秒)

    Rhodamine derivatives and the use thereof
    42.
    发明授权
    Rhodamine derivatives and the use thereof 有权
    罗丹明衍生物及其用途

    公开(公告)号：US06184379B2

    公开(公告)日：2001-02-06

    申请号：US09324265

    申请日：1999-06-02

    申请人： Hans-Peter Josel ,  Rupert Herrmann ,  Dieter Heindl ,  Klaus Muhlegger ,  Gregor Sagner ,  Karl Heinz Drexhage ,  Jorg Frantzeskos ,  Jutta Arden-Jacob

    发明人： Hans-Peter Josel ,  Rupert Herrmann ,  Dieter Heindl ,  Klaus Muhlegger ,  Gregor Sagner ,  Karl Heinz Drexhage ,  Jorg Frantzeskos ,  Jutta Arden-Jacob

    IPC分类号： C07D49147

    CPC分类号： C09B11/24 ,  Y10S436/80

    摘要： The invention concerns rhodamine derivatives of the general formulae in which Ca-Cd each denote a C atom, and Ca and Cb as well as Cc and Cd are either linked together by a single bond or by a double bond; X1 to X16 denote independently of one another halogen, sulfonic acid, hydrogen or an alkyl residue with 1-20 C atoms in which the alkyl residue can be substituted with one or several halogen or sulfonic acid residues; R1 and R2 are either identical or different and denote either hydrogen, alkyl with 1-20 C atoms, polyoxyhydrocarbyl units, phenyl or phenylalkyl with 1-3 carbon atoms in the alkyl chain in which the alkyl and/or phenyl residues can be substituted by one or several hydroxy, halogen, sulfonic acid, amino, carboxy or alkoxycarbonyl groups where alkoxy can have 1-4 carbon atoms, R1 contains at least one activatable group, R2 and X4 can be optionally linked together via a bridge composed of 0-2 C atoms. In contrast to the prior art, these compounds are characterized in that A1, A2 and A3 can independently of one another denote hydrogen, cyano, halogen and sulfonic acid; B1 denotes either halogen, cyano or hydrogen; B2 denotes hydrogen, amide, halogen and an alkyl residue with 1-20 C atoms. In addition the invention concerns activated rhodamine derivatives, correspondingly conjugated biomolecules and their use in diagnostic systems.

    摘要翻译： 本发明涉及一般式的罗丹明衍生物，其中Ca-Cd各自表示C原子，Ca和Cb以及C c和Cd通过单键或双键连接在一起; X1至X16彼此独立地表示卤素，磺酸，氢或具有1-20个C原子的烷基残基，其中烷基残基可被一个或几个卤素或磺酸残基取代; R 1和R 2可以相同或不同，表示氢，具有1-20个C原子的烷基，聚氧烃基单元，烷基链中具有1-3个碳原子的苯基或苯基烷基，其中烷基和/或苯基残基可被 一个或几个羟基，卤素，磺酸，氨基，羧基或烷氧基羰基，其中烷氧基可以具有1-4个碳原子，R 1包含至少一个可活化基团，R 2和X 4可以任选地通过由0-2 C原子。 与现有技术相反，这些化合物的特征在于，A1，A2和A3可以彼此独立地表示氢，氰基，卤素和磺酸; B1表示卤素，氰基或氢; B2表示氢，酰胺，卤素和具有1-20个C原子的烷基。 此外，本发明涉及活化的罗丹明衍生物，相应的共轭生物分子及其在诊断系统中的用途。

    Stable NAD/NADH derivatives
    44.
    发明授权
    Stable NAD/NADH derivatives 有权
    稳定的NAD / NADH衍生物

    公开(公告)号：US08809013B2

    公开(公告)日：2014-08-19

    申请号：US12020244

    申请日：2008-01-25

    申请人： Dieter Heindl ,  Joachim Hoenes ,  Carina Horn ,  Claudia Gaessler-Dietsche

    发明人： Dieter Heindl ,  Joachim Hoenes ,  Carina Horn ,  Claudia Gaessler-Dietsche

    IPC分类号： C12Q1/32

    CPC分类号： C12Q1/32

    摘要： The present invention provides for stable nicotinamide adenine dinucleotide (NAD/NADH) and nicotinamide adenine dinucleotide phosphate (NADP/NADPH) derivatives of formula (I), enzyme complexes of these derivatives and their use in biochemical detection methods and reagent kits.

    摘要翻译： 本发明提供式（I）的稳定的烟酰胺腺嘌呤二核苷酸（NAD / NADH）和烟酰胺腺嘌呤二核苷酸磷酸（NADP / NADPH）衍生物，这些衍生物的酶复合物及其在生物化学检测方法和试剂盒中的用途。

    Nucleic acid amplification in the presence of modified randomers
    45.
    发明授权
    Nucleic acid amplification in the presence of modified randomers 失效
    在改良的随机存在下进行核酸扩增

    公开(公告)号：US08592184B2

    公开(公告)日：2013-11-26

    申请号：US13711846

    申请日：2012-12-12

    申请人： Dieter Heindl ,  Waltraud Ankenbauer ,  Frank Laue

    发明人： Dieter Heindl ,  Waltraud Ankenbauer ,  Frank Laue

    IPC分类号： C12P19/34 ,  C07H21/00

    CPC分类号： C12P19/34 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q2525/179 ,  C12Q2525/101

    摘要： The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.

    摘要翻译： 本发明涉及包含优选热稳定性的DNA聚合酶，脱氧核苷酸，至少一种引物寡核苷酸或一对扩增引物和随机化的5-8位寡核苷酸的组合物，其特征在于所述寡核苷酸包含与有机 疏水部分这样的组合物特别适用于进行热启动PCR。

    CONJUGATE BETWEEN A THIOPHILIC SOLID PHASE AND AN OLIGONUCLEOTIDE COMPRISING A THIOOXONUCLEOTIDE
    46.
    发明申请
    CONJUGATE BETWEEN A THIOPHILIC SOLID PHASE AND AN OLIGONUCLEOTIDE COMPRISING A THIOOXONUCLEOTIDE 有权
    一个硫代固体相与一个含有硫代核苷酸的寡核苷酸之间的结合

    公开(公告)号：US20130210009A1

    公开(公告)日：2013-08-15

    申请号：US13866412

    申请日：2013-04-19

    申请人： Simone Budow ,  Ping Ding ,  Dieter Heindl ,  Alfons Nichtl ,  Frank Seela

    发明人： Simone Budow ,  Ping Ding ,  Dieter Heindl ,  Alfons Nichtl ,  Frank Seela

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  C07H21/02

    CPC分类号： C12Q1/6876 ,  C07H21/02

    摘要： An oligonucleotide-solid phase conjugate, wherein the solid phase is thiophilic and the oligonucleotide comprises at least one thiooxonucleobase according to Formula I, wherein X is CH or N, R1 is H or NH2, --- indicates a covalent bond, and said oligonucleotide is bound to said solid phase via the sulfur atom of said thiooxonucleotide. Also disclosed are methods for producing such conjugate as well as various uses for such oligonucleotide metal conjugate.

    摘要翻译： 一种寡核苷酸 - 固相缀合物，其中所述固相是亲核的，并且所述寡核苷酸包含至少一种根据式I的硫氧基核碱基，其中X为CH或N，R 1为H或NH 2，表示共价键，所述寡核苷酸 通过所述硫代核苷酸的硫原子与所述固相结合。 还公开了用于生产这种缀合物的方法以及这种寡核苷酸金属缀合物的各种用途。

    Thermostable Taq polymerase fragment
    50.
    发明授权
    Thermostable Taq polymerase fragment 有权
    热稳定性Taq聚合酶片段

    公开(公告)号：US07972830B2

    公开(公告)日：2011-07-05

    申请号：US10917157

    申请日：2004-08-12

    申请人： Thomas Meier ,  Waltraud Ankenbauer ,  Annette Deufel ,  Dieter Heindl ,  Gisela Betzl ,  Rainer Schmuck ,  Bernd Schneidinger ,  Jessica Strey

    发明人： Thomas Meier ,  Waltraud Ankenbauer ,  Annette Deufel ,  Dieter Heindl ,  Gisela Betzl ,  Rainer Schmuck ,  Bernd Schneidinger ,  Jessica Strey

    IPC分类号： C12N9/12

    CPC分类号： C12N9/1252

    摘要： It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (TaqΔ288) possesses an increased thermostability over TaqWT, TaqΔ279, and TaqΔ289. The present invention therefore provides TaqΔ288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for TaqΔ288. The invention also encompasses kits containing TaqΔ288 as well as the use of TaqΔ288 and kits containing TaqΔ288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.

    摘要翻译： 发现缺乏288个N-末端氨基酸（Taq＆Dgr; 288）的天然的水生栖热菌水生DNA聚合酶（TaqWT）的片段具有比TaqWT，Taq＆Dgr; 279和Taq＆Dgr; 289增加的热稳定性。 因此，本发明提供Taq＆Dgr; 288，编码其相同或衍生物的重组表达载体，以及Taq＆Dgr; 288的纯化方案。 本发明还包括含有Taq＆Dgr; 288的试剂盒，以及使用Taq＆Dgr; 288和含有Taq＆Dgr; 288的试剂盒。 此外，本发明包括用于测序核酸模板的方法和用于扩增靶核酸的方法。