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    CITRUS TRISTEZA VIRUS BASED VECTORS FOR FOREIGN GENE/S EXPRESSION
    43.
    发明申请
    CITRUS TRISTEZA VIRUS BASED VECTORS FOR FOREIGN GENE/S EXPRESSION 有权
    CITRUS TRISTEZA病毒基因外源基因/ S表达载体

    公开(公告)号：US20150096078A1

    公开(公告)日：2015-04-02

    申请号：US14269637

    申请日：2014-05-05

    申请人： University of Florida Research Foundation, Inc.

    发明人： William O. Dawson ,  Svetlana Folimonova ,  Choaa Amine El Mohtar ,  Siddarame Gowda ,  Subhas Hajeri

    IPC分类号： C12N7/00 ,  C12N15/82

    CPC分类号： C12N7/00 ,  C12N15/8203 ,  C12N2770/00043 ,  C12N2770/00045

    摘要： Disclosed herein are viral vectors based on modifications of the Citrus Tristeza virus useful for transfecting citrus trees for beneficial purposes. Included in the disclosure are viral vectors including one or more gene cassettes that encode heterologous polypeptide s. The gene cassettes are positioned at desirable locations on the viral genome so as to enable expression while preserving functionality of the virus. Also disclosed are methods of transfecting plants and plants transfected with viral vector embodiments.

    摘要翻译： 本文公开了基于用于转化柑橘树以用于有益目的的柑橘属Tristeza病毒的修饰的病毒载体。 本公开中包括病毒载体，包括编码异源多肽的一个或多个基因盒。 将基因盒定位在病毒基因组上的期望位置，以便能够表达同时保持病毒的功能。 还公开了用病毒载体实施方案转染植物和植物的方法。

    System for expression of genes in plants
    44.
    发明授权
    System for expression of genes in plants 有权

    公开(公告)号：US08597942B2

    公开(公告)日：2013-12-03

    申请号：US13243796

    申请日：2011-09-23

    申请人： Oleg Fedorkin ,  Shailaja Rabindran ,  Vidadi Yusibov

    发明人： Oleg Fedorkin ,  Shailaja Rabindran ,  Vidadi Yusibov

    IPC分类号： C12N7/01

    CPC分类号： C12N15/8203 ,  C07K14/61 ,  C07K14/62 ,  C12N7/00 ,  C12N15/8257 ,  C12N2770/00043 ,  C12P21/02

    摘要： The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants.

    Viral based transient-expression vector system that allows multiple applications
    45.
    发明授权
    Viral based transient-expression vector system that allows multiple applications 有权
    基于病毒的瞬态表达载体系统，允许多种应用

    公开(公告)号：US08389804B2

    公开(公告)日：2013-03-05

    申请号：US13207731

    申请日：2011-08-11

    申请人： William O. Dawson ,  Svetlana Y. Folimonova

    发明人： William O. Dawson ,  Svetlana Y. Folimonova

    IPC分类号： C12N15/82 ,  C12N15/33

    CPC分类号： C12N15/8203 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/00034 ,  C12N2770/00043

    摘要： Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease. Specifically exemplified herein are methods of transfecting woody trees that allow multiple applications of vectors while avoiding superinfection exclusion.

    摘要翻译： 本文公开了适于转染木本树的病毒载体，用于递送和表达有益基因。 本文具体举例说明用于转染柑橘树的载体。 载体允许表达有用的蛋白质，例如可以保护树免于疾病的蛋白质。 具体举例说明的是转染木本树的方法，其允许多次施用载体同时避免超感染排除。

    VIRUS VECTOR AND USE THEREOF
    46.
    发明申请
    VIRUS VECTOR AND USE THEREOF 有权
    病毒矢量及其使用

    公开(公告)号：US20110107466A1

    公开(公告)日：2011-05-05

    申请号：US12450999

    申请日：2008-04-11

    申请人： Masashi Mori ,  Koji Dohi

    发明人： Masashi Mori ,  Koji Dohi

    IPC分类号： C07H21/04 ,  C12N15/63 ,  A01H5/00 ,  C12N5/10 ,  C12P21/00

    CPC分类号： C12N15/8203 ,  C12N15/8257 ,  C12N2770/00043

    摘要： It is intended to provide a polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein, and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, the linking site being located downstream of the second base sequence, the second base sequence being obtained by modifying with a base sequence in a native sequence derived from a virus by insertion, substitution, or addition. By using this, a vector containing a viral base sequence is constructed, and a protein is efficiently produced without worsening growth of a host cell containing the vector.

    摘要翻译： 旨在提供包含病毒碱基序列的多核苷酸，所述病毒碱基序列包含编​​码病毒复制蛋白的第一碱基序列和编码病毒运动蛋白的第二碱基序列，所述第二碱基序列位于第一 碱基序列并具有与编码待表达多肽的外源碱基序列连接的连接位点，所述连接位点位于第二碱基序列的下游，第二碱基序列通过用天然序列衍生的碱基序列修饰获得 从病毒通过插入，替换或添加。 通过使用该方法构建含有病毒碱基序列的载体，能有效地产生蛋白质，而不会使包含载体的宿主细胞的生长恶化。