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    Production of isoprenoids
    45.
    发明授权
    Production of isoprenoids 有权
    生产类异戊二烯

    公开(公告)号：US09102954B2

    公开(公告)日：2015-08-11

    申请号：US13865072

    申请日：2013-04-17

    申请人： Arkion Life Sciences, LLC

    发明人： James R. Millis ,  Julie Maurina-Brunker ,  Thomas W. McMullin

    IPC分类号： C12N1/14 ,  C12N15/00 ,  C12N9/04 ,  C12N9/12 ,  C12N9/88 ,  C07H21/04 ,  C12P7/04 ,  C12N15/81 ,  C07C403/08 ,  C07C403/24 ,  C07D311/72 ,  C12N9/02 ,  C12N9/10 ,  C12P9/00 ,  C12P17/06 ,  C12P23/00 ,  H01Q1/00

    CPC分类号： C12P7/04 ,  C07C403/08 ,  C07C403/24 ,  C07C2601/16 ,  C07D311/72 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/1085 ,  C12N15/81 ,  C12P9/00 ,  C12P17/06 ,  C12P23/00 ,  C12Y101/01034 ,  H01Q1/00

    摘要： A recombinant yeast that makes an isoprenoid compound is provided. The yeast comprises an endogenous mevalonate pathway comprising (i) an enzyme that converts acetyl-CoA to acetoacetyl-CoA, (ii) an enzyme that converts acetoacetyl-CoA to hydroxymethylglutaryl-CoA, (iii) an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid, (iv) an enzyme that converts mevalonic acid to phosphomevalonic acid, (v) an enzyme that converts phosphomevalonic acid to phosphomevalonate, and (vi) an enzyme that converts phosphomevalonate to isopentenyl pyrophosphate. The yeast further comprises heterologous nucleic acid sequences encoding an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid, an enzyme that converts mevalonic acid to phosphomevalonic acid, an enzyme that converts phosphomevalonic acid to phosphomevalonate, and an enzyme that converts phosphomevalonate to isopentenyl pyrophosphate. Carbon flow through the mevalonate pathway is increased in the recombinant yeast compared to a yeast that does not comprise the heterologous nucleic acid molecule encoding an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid.

    摘要翻译： 提供了制备类异戊二烯化合物的重组酵母。 酵母包含内源性甲羟戊酸途径，其包含（i）将乙酰辅酶A转化为乙酰乙酰辅酶A的酶，（ii）将乙酰乙酰辅酶A转化为羟甲基戊二酰辅酶A的酶，（iii）将羟甲基戊二酰辅酶A转化成甲羟戊酸 酸，（iv）将甲羟戊酸转化为磷酸戊糖酸的酶，（v）将磷酸戊糖醛酸转化为磷酸戊糖醛酸的酶，以及（vi）将磷酸戊糖转化为异戊烯焦磷酸的酶。 酵母进一步包含编码将羟甲基戊二酰辅酶A转化为甲羟戊酸的酶的异源核酸序列，将甲羟戊酸转化为磷酸戊糖酸的酶，将磷酸戊糖醛酸转化为磷酸甲羟戊酸的酶，以及将磷酸戊糖转化为异戊烯基焦磷酸的酶。 与不含编码将羟甲基戊二酰辅酶A转化为甲羟戊酸的酶的异源核酸分子的酵母相比，重组酵母中的碳流量增加。

    GENETICALLY MODIFIED ORGANISM FOR THE PRODUCTION OF LIPIDS
    46.
    发明申请
    GENETICALLY MODIFIED ORGANISM FOR THE PRODUCTION OF LIPIDS 有权
    用于生产脂肪的基因改造有机体

    公开(公告)号：US20150024009A1

    公开(公告)日：2015-01-22

    申请号：US14491212

    申请日：2014-09-19

    申请人： Christine Lang ,  Andreas Raab

    发明人： Christine Lang ,  Andreas Raab

    IPC分类号： C12P7/64 ,  C12N9/04 ,  C12P5/00 ,  C12N15/81

    CPC分类号： C12P7/6463 ,  A61K8/99 ,  A61Q19/00 ,  C12N9/0006 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/1029 ,  C12N15/81 ,  C12P5/007 ,  C12P7/6436 ,  C12P7/649 ,  C12P33/00 ,  C12Y101/01034 ,  C12Y203/0102 ,  C12Y203/01026 ,  Y02E50/343 ,  Y02P20/52

    摘要： The invention provides an isolated genetically modified non-mammalian organism, wherein the activity of acyl-CoA:sterol acyltransferase/sterol O-acyltransferase (EC 2.3.1.26) and/or diacylglycerol acyltransferase/diacylglycerol O-acyltranferase (EC 2.3.1.20) and/or lecithin cholesterol acyl transferase/phospholipid: diacylglycerol acyltransferase (EC 2.3.1.158) and/or acyl CoA-wax alcohol acyltransferase (EC 2.3.1.75) is reduced or abolished in comparison with a corresponding wildtype organism, methods of use of such an organism, shuttle vehicles for making such an organism and methods for producing such an organism.

    摘要翻译： 本发明提供了一种分离的遗传修饰的非哺乳动物生物体，其中酰基辅酶A.固醇酰基转移酶/固醇O-酰基转移酶（EC 2.3.1.26）和/或二酰基甘油酰基转移酶/二酰基甘油O-酰基转移酶（EC 2.3.1.20）和 /或卵磷脂胆固醇酰基转移酶/磷脂：二酰基甘油酰基转移酶（EC 2.3.1.158）和/或酰基辅酶A蜡醇酰基转移酶（EC 2.3.1.75）与相应的野生型生物相比被减少或消除，使用这种 生物体，用于制造这种生物体的穿梭载体和用于生产这种生物体的方法。