公开(公告)号：US12221492B2

公开(公告)日：2025-02-11

申请号：US17149830

申请日：2021-01-15

Applicant: Cytiva BioProcess R&D AB

Inventor： Gustav Rodrigo ,  Tomas Bjorkman ,  Mats Ander

IPC: C07K16/46 ,  B01D15/38 ,  B01J20/289 ,  B01J20/32 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K16/06

Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.

公开(公告)号：US20240402142A1

公开(公告)日：2024-12-05

申请号：US18748622

申请日：2024-06-20

Applicant: CYTIVA BIOPROCESS R&D AB

Inventor： Klaus Gebauer ,  Dan Hermansson

IPC: G01N30/60 ,  B01D15/20 ,  B01D15/22 ,  G01N30/56

Abstract: A method for packing a chromatography column with chromatography media, comprising the steps of: providing a system comprising a column tube having a closed first end comprising an inlet/outlet, a media inlet adjacent a second end of the column tube and an adaptor positioned inside the column tube initially adjacent the second end of the column tube for sliding and sealing contact with an inner face of the column tube, the column tube and adaptor arranged initially such that they define an internal volume and such that the media inlet is in fluid connection with the internal volume; connecting a media slurry source to the media inlet; at least partially filling the internal volume with media slurry via the media inlet; forcing the adaptor towards the first end of the column tube to reduce the internal volume such that the media inlet is no longer in fluid connection with the reduced internal volume.

公开(公告)号：US20240294568A1

公开(公告)日：2024-09-05

申请号：US18549552

申请日：2022-04-11

Applicant: Cytiva BioProcess R&D AB

Inventor： Gustav Rodrigo

IPC: C07K1/36

CPC classification number: C07K1/36

Abstract: Method of clarifying a crude protein solution, the method comprising to provide (100) a volume of the crude protein solution, mixing (101) Na2HPO4 and CaCl2) in water, forming a first solution, adding (102) the first solution to the volume of crude protein solution, thereby forming a second solution. NaCl is added to the first solution, the crude protein solution and/or to the second solution. The second solution is mixed (103). The thus formed flocculated material is separated (104) from the second solution, obtaining a clarified protein solution. The clarified protein solution may thereafter be purified by chromatography.

公开(公告)号：US11911711B2

公开(公告)日：2024-02-27

申请号：US17938653

申请日：2022-10-06

Applicant: Cytiva BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: B01D15/14 ,  B01D15/18 ,  B01D15/22 ,  G01N30/46 ,  G01N30/60 ,  G01N30/88

CPC classification number: B01D15/14 ,  B01D15/1864 ,  B01D15/1885 ,  B01D15/22 ,  G01N30/466 ,  G01N30/6043 ,  G01N30/6091 ,  G01N30/6039 ,  G01N2030/8881 ,  Y10T29/49826

Abstract: A parallel assembly of chromatography column modules, the assembly having one common assembly inlet and one common assembly outlet, each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet, wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

公开(公告)号：US20230331870A1

公开(公告)日：2023-10-19

申请号：US18093624

申请日：2023-01-05

Applicant: Cytiva Bioprocess R&D AB

Inventor： Gustav RODRIGO ,  Tomas Bjorkman ,  Mats Ander

IPC: C07K16/46 ,  B01J20/289 ,  B01J20/32 ,  B01D15/38 ,  C07K16/06 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00

CPC classification number: C07K16/46 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3274 ,  B01D15/3809 ,  C07K16/065 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00

Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:22, SEQ ID NO 51 or SEQ ID NO 52 wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO:4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.

公开(公告)号：US20230257417A1

公开(公告)日：2023-08-17

申请号：US18181974

申请日：2023-03-10

Applicant: Cytiva BioProcess R&D AB

Inventor： Annika Kristina Forss ,  Gustav Jose Rodrigo ,  Tomas Bjorkman ,  Jesper Ulf Hansson ,  Mats Ander

IPC: C07K1/22 ,  C07K16/12 ,  C07K17/10 ,  B01J20/286 ,  C07K14/31 ,  C07K16/06 ,  B01D15/00 ,  B01J20/32

CPC classification number: C07K1/22 ,  C07K16/1271 ,  C07K17/10 ,  B01J20/286 ,  C07K14/31 ,  C07K16/065 ,  B01D15/00 ,  B01J20/3274 ,  B01J20/3212 ,  B01J2220/52

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support: b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing said separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA).

公开(公告)号：US11667671B2

公开(公告)日：2023-06-06

申请号：US16911511

申请日：2020-06-25

Applicant: Cytiva BioProcess R&D AB

Inventor： Annika Forss ,  Mats Ander ,  Tomas Bjorkman ,  Hans Blom ,  Jesper Hansson ,  Gustav Rodrigo

IPC: C07K1/16 ,  C07K1/22 ,  C07K16/00 ,  B01J20/286 ,  B01J20/285 ,  B01D15/18 ,  B01D15/34 ,  C07K14/31

CPC classification number: C07K1/165 ,  B01D15/1807 ,  B01D15/1864 ,  B01D15/34 ,  B01J20/285 ,  B01J20/286 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2319/30

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of:



a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml;
b) contacting a liquid sample comprising an immunoglobulin with the separation matrix;
c) washing the separation matrix with a washing liquid;
d) eluting the immunoglobulin from the separation matrix with an elution liquid; and
e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

公开(公告)号：US20220032266A1

公开(公告)日：2022-02-03

申请号：US17296684

申请日：2019-12-11

Applicant: Cytiva BioProcess R&D AB

Inventor： David Jansson ,  Jesper Hansson ,  Anna Akerblom ,  Emma Michaelsson

IPC: B01J20/24 ,  C08J3/075 ,  C08J3/24 ,  C12N7/00 ,  B01J20/285 ,  B01J20/06 ,  B01J20/28

Abstract: The present invention relates to porous cross-linked agarose gel beads which have a low agarose content, a method for the preparation of the beads and their use in chromatographic applications. The beads are suitable for the separation/purification of biomolecules from a biological sample. Due to the high porosity of the beads, they are especially suitable for separation/isolation of larger particles, such as virus particles e.g. adeno virus.

公开(公告)号：US11136357B2

公开(公告)日：2021-10-05

申请号：US16252015

申请日：2019-01-18

Applicant: Cytiva BioProcess R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Tomas Bjorkman

IPC: C07K14/195 ,  C07K1/22 ,  C07K16/00

Abstract: A kappa light chain-binding polypeptide comprising or consisting essentially of one or more mutated binding domains of Peptostreptococcus Protein L.

公开(公告)号：US20210148870A1

公开(公告)日：2021-05-20

申请号：US17156939

申请日：2021-01-25

Applicant: CYTIVA BIOPROCESS R&D AB

Inventor： Klaus Gebauer

IPC: G01N30/60 ,  B01D15/22 ,  G01N30/86 ,  G01N30/56 ,  B01D15/20 ,  B01D15/18

Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.