-
51.发明授权公开(公告)号：US09663559B2
公开(公告)日：2017-05-30
申请号：US15063471
申请日：2016-03-07
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
CPC classification number: B01J20/24 , B01D15/3809 , B01J20/28019 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , B01J2220/54 , C07K1/22 , C07K14/31 , C07K16/00 , C07K17/00 , C07K17/10 , C07K2317/55
Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
-
公开(公告)号：US20170097327A1
公开(公告)日：2017-04-06
申请号：US15379447
申请日：2016-12-14
Applicant: GE Healthcare BioProcess R&D AB
Inventor： KLAUS GEBAUER
CPC classification number: G01N30/6004 , B01D15/18 , B01D15/22 , G01N30/6017 , G01N30/6021 , G01N30/6052 , G01N30/606
Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.
-
公开(公告)号：US09573973B2
公开(公告)日：2017-02-21
申请号：US14396207
申请日：2013-04-22
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Jesper Hansson , Gustav Rodrigo , Tobias E Soderman
IPC: B01D15/36 , C07K1/22 , B01D15/18 , B01J20/286 , B01J20/32 , B01D15/38 , B01J20/26 , B01J20/28 , B01J20/289 , B01J20/30 , C07H1/08 , G01N30/88
CPC classification number: B01J39/26 , B01D15/1864 , B01D15/34 , B01D15/361 , B01D15/362 , B01D15/3809 , B01J20/24 , B01J20/261 , B01J20/265 , B01J20/267 , B01J20/28014 , B01J20/28033 , B01J20/286 , B01J20/289 , B01J20/3085 , B01J20/3212 , B01J20/3278 , B01J20/3282 , B01J2220/80 , C07H1/08 , C07K1/22 , G01N2030/8827 , Y02P20/582
Abstract: The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.
Abstract translation: 本发明公开了一种从液体中的至少一种其它组分分离生物分子的方法，包括使所述液体与包含固体支持物和与所述固体支持物结合的聚合物链的分离基质接触的步骤。 聚合物链包含衍生自结构为CH 2 = CH-L-X的第一单体的单元，其中L为共价键或包含2-6个碳原子的烷基醚或羟基取代的烷基醚链，X为磺酸盐或膦酸酯基团。
-
公开(公告)号：US10933350B2
公开(公告)日：2021-03-02
申请号：US16154639
申请日：2018-10-08
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Klaus Gebauer
Abstract: A parallel assembly of chromatography column modules, the assembly having one common assembly inlet and one common assembly outlet, each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet, wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.
-
公开(公告)号：US20200318120A1
公开(公告)日：2020-10-08
申请号：US16615552
申请日：2018-05-23
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Mats ANDER , Magnus BERGMAN , Tomas BJORKMAN , Joakim GALLI , Gustav RODRIGO
Abstract: A recombinant protein comprising a functional polypeptide and, linked to the N-terminus of said functional polypeptide, an N-terminal spacer having a length such that the number of amino acid residues between a signal peptide cleaving site and an N-terminus proximal structural unit of said functional polypeptide is 14-24.
-
Patent Agency Ranking
公开(公告)号：US20200299326A1
公开(公告)日：2020-09-24
申请号：US16893574
申请日：2020-06-05
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav José Rodrigo , Tomas Bjorkman , Mats Ander , Jesper Ulf Hansson
IPC: C07K1/22 , C07K16/00 , C07K14/31 , B01J20/28 , B01J20/24 , B01D15/38 , C07K17/10 , B01J20/286 , B01J20/32 , C07K16/06
Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
-
公开(公告)号：US10751645B2
公开(公告)日：2020-08-25
申请号：US15882998
申请日：2018-01-29
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Carina Engstrand , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J Johansson , Jean-Luc Maloisel
IPC: B01D15/38 , B01D15/32 , B01D15/36 , B01J20/32 , B01J20/286 , B01J41/20 , C07K1/36 , C07K16/06 , C07K1/16 , C07K16/00
Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
-
公开(公告)号：US10711035B2
公开(公告)日：2020-07-14
申请号：US16682855
申请日：2019-11-13
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav José Rodrigo , Tomas Bjorkman , Mats Ander , Jesper Ulf Hansson
IPC: A23J1/00 , C07K1/22 , C07K16/00 , C07K14/31 , B01J20/28 , B01J20/24 , B01J20/32 , B01D15/38 , C07K17/10 , B01J20/286 , C07K16/06
Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
-
公开(公告)号：US10654887B2
公开(公告)日：2020-05-19
申请号：US15282367
申请日：2016-09-30
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Gustav Jose Rodrigo , Tomas Bjorkman , Mats Ander , Jesper Ulf Hansson
IPC: C07K1/22 , B01J20/26 , C07K16/00 , C07K14/31 , B01J20/32 , B01J20/285 , B01J20/286 , C07K16/12 , C07K17/10
Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
-
公开(公告)号：US20200079878A1
公开(公告)日：2020-03-12
申请号：US16594731
申请日：2019-10-07
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:22, SEQ ID NO 51 or SEQ ID NO 52 wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO:4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
-
-
-
-
-
-
-
-
-
Search Results
92
records
(took 0.028 seconds)
