-
公开(公告)号：US09347091B2
公开(公告)日：2016-05-24
申请号：US13862530
申请日：2013-04-15
CPC分类号： C12Q1/6841 , C12Q1/6876 , C12Q1/6886 , C12Q2565/1025 , C12Q2563/107
摘要： The present disclosure relates to a set of at least 100 single-stranded oligonucleotide probes directed against (or complementary to) portions of a genomic target sequence of interest. The present disclosure also relates to a method of detecting a genomic target sequence of interest using the set of oligonucleotide probes and a method of generating the set of oligonucleotide probes. Further, the present disclosure relates to a kit comprising the set of oligonucleotide probes and at least one further component.
-
公开(公告)号：US08101744B2
公开(公告)日：2012-01-24
申请号：US12105556
申请日：2008-04-18
IPC分类号： C07H21/00
CPC分类号： C12N15/1006
摘要： Disclosed are water-soluble ionic liquids suitable for promoting adsorption of nucleic acids to a solid phase. The use thereof, particularly methods for the isolation of nucleic acids from an aqueous solution, as well as kits for performing those methods are disclosed.
摘要翻译： 公开了适用于促进核酸吸附到固相的水溶性离子液体。 公开了其用途，特别是用于从水溶液中分离核酸的方法，以及用于实施这些方法的试剂盒。
-
公开(公告)号：US20110053251A1
公开(公告)日：2011-03-03
申请号：US12855803
申请日：2010-08-13
摘要： The invention provides a composition containing an aqueous liquid phase, bacterial cells, and an ionic compound dissolved in the liquid phase. The ionic compound is selected from the group consisting of 1-butyl-3-methyl-imidazolium-thiocyanate, 1-butyl-3-methyl-imidazolium-2(2-methoxy-ethoxy)ethylsulfate, 1-methyl-1-[4-(3-methyl-3H-imidazol-1-ium)-but-1-yl]-3H-imidazolium-di(toluylsulfate), and 1-butyl-3-methyl-imidazolium-octylsulfate. The compositions of the invention are advantageously used for preparing lysates of biological cells, particularly bacterial cells.
摘要翻译： 本发明提供一种含有水相液相，细菌细胞和溶于液相的离子化合物的组合物。 离子化合物选自1-丁基-3-甲基 - 咪唑鎓 - 硫氰酸酯，1-丁基-3-甲基 - 咪唑鎓-2（2-甲氧基 - 乙氧基）乙基硫酸酯，1-甲基-1- [4 - （3-甲基-3H-咪唑-1-基） - 丁-1-基] -3H-咪唑鎓 - 二（甲苯基硫酸盐）和1-丁基-3-甲基 - 咪唑鎓辛基硫酸盐。 本发明的组合物有利地用于制备生物细胞，特别是细菌细胞的裂解物。
-
公开(公告)号：US20100285603A1
公开(公告)日：2010-11-11
申请号：US12326972
申请日：2008-12-03
申请人： Uwe Kobold , Thomas Duelffer , Michael Grol , Rupert Herrmann , Herbert Von der Eltz , Leopold Von Proff
发明人： Uwe Kobold , Thomas Duelffer , Michael Grol , Rupert Herrmann , Herbert Von der Eltz , Leopold Von Proff
IPC分类号： G01N33/00
CPC分类号： G01N33/82 , Y10T436/20 , Y10T436/203332
摘要： The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.
摘要翻译： 本发明涉及一种测量样品中维生素D代谢物的方法，所述方法包括以下步骤：（a）在适于从维生素D结合物释放维生素D代谢物的条件下用维生素D代谢物释放试剂处理所述样品 蛋白质，不会引起蛋白质沉淀，（b）使步骤（a）中得到的处理样品进行色谱分离，和（c）在所述色谱分离期间或之后测量维生素D代谢物。 本发明还涉及确定用于疾病诊断的受试者的维生素D状态的方法以及用于实施本发明的方法的试剂和试剂盒。
-
55.发明授权Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification 有权苯并咪唑鎓化合物和苯并咪唑化合物的盐用于核酸扩增公开(公告)号：US07781165B2
公开(公告)日：2010-08-24
申请号：US11581735
申请日：2006-10-16
IPC分类号： C12Q1/68 , C12P19/34 , C07D235/00
CPC分类号： C12Q1/6844 , C12Q2527/125
摘要： The present invention is directed to the use of a benzimidazolium compound comprising a side chain at at least one of its N-residues, said chain being either a Cn-alkyl or a substituted Cn-alkyl, characterized in that preferably n is ≧3, as an additive for a nucleic acid amplification reaction.
摘要翻译： 本发明涉及在其N-残基中的至少一个上含有侧链的苯并咪唑化合物的用途，所述链是C 1 - 烷基或取代的C n - 烷基，其特征在于n优选≥3， 作为核酸扩增反应的添加剂。
-
公开(公告)号：US20090246866A1
公开(公告)日：2009-10-01
申请号：US12327854
申请日：2008-12-04
IPC分类号： C12M3/08
CPC分类号： G01N33/5002
摘要： The present invention relates to a sampling tube for collecting and processing a whole blood sample. The sampling tube contains a reagent for differential hemolysis of whole blood, wherein said reagent for differential hemolysis comprises a chemical for differential hemolysis and an anti-coagulant, and wherein said sampling tube is a ready-to-use and single-use sampling tube. It also relates to the use of said sampling tube in the processing of a whole blood sample for liquid chromatography and also to the use of a blood sample processed in such sampling tube in a liquid chromatography-based analysis.
摘要翻译： 本发明涉及一种用于收集和处理全血样本的取样管。 采样管含有用于全血差异溶血的试剂，其中所述用于差异溶血的试剂包括用于差异溶血的化学药剂和抗凝血剂，并且其中所述采样管是即用型和一次性采样管。 它还涉及在液相色谱法的全血样品的处理中使用所述采样管以及在基于液相色谱的分析中使用在这种采样管中处理的血液样品。
-
57.发明授权N8- and C8-linked purine bases and structurally related heterocycles as universal nucleosides used for oligonucleotides hybridization 有权N8和C8连接的嘌呤碱基和结构相关杂环作为用于寡核苷酸杂交的通用核苷公开(公告)号：US07566775B2
公开(公告)日：2009-07-28
申请号：US11338149
申请日：2006-01-23
摘要： The present invention is directed to a nucleic acid binding compound comprising N8- or C8-linked purine bases or structurally related heterocycles, a compound useful for the preparation of such compound, a binding product of this nucleic acid binding compound with a nucleic acid, a method for the determination of a nucleic acid using said compound, and several uses of 8-linked purine bases and structurally related heterocycles. Compounds according to the present invention have advantageous properties when used in hybridization methods.
摘要翻译： 本发明涉及包含N8-或C8-连接的嘌呤碱基或结构相关杂环的核酸结合化合物，可用于制备该化合物的化合物，该核酸结合化合物与核酸的结合产物， 使用所述化合物测定核酸的方法以及8-连接的嘌呤碱基和结构相关杂环的几种用途。 当用于杂交方法时，根据本发明的化合物具有有利的性质。
-
公开(公告)号：US07276592B2
公开(公告)日：2007-10-02
申请号：US10816298
申请日：2004-04-01
CPC分类号： A61K31/513 , A61K31/52 , A61K31/675 , C07D405/04 , C07D495/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H21/00 , C07H21/04 , C12P19/34 , C12P19/38 , C12Q1/68 , G01N33/53 , G01N33/566 , G01N33/58 , G01N37/00 , Y02P20/55
摘要： The present invention is related to compounds comprising six-membered rings capable of being incorporated into nucleic acids. Particularly, the six-membered ring is a derivative of cyclohexane, cyclohexene, tetrahydropyran, tetrahydrothiopyran or piperidine. These compounds may be used to build up oligomeric compounds. The invention is further related to uses of these oligomeric compounds for hybridization and as probes. In addition, methods for the detection of nucleic acids are provided wherein the oligomeric compounds are used.
摘要翻译： 本发明涉及能够并入核酸中的包含六元环的化合物。 特别地，六元环是环己烷，环己烯，四氢吡喃，四氢噻喃或哌啶的衍生物。 这些化合物可用于构建低聚化合物。 本发明还涉及这些寡聚化合物用于杂交和作为探针的用途。 此外，提供了检测核酸的方法，其中使用低聚化合物。
-
公开(公告)号：US20070149565A1
公开(公告)日：2007-06-28
申请号：US11627572
申请日：2007-01-26
申请人： Gerald Sigler , Raymond Hui , Ina Deras , Richard Root , Mitali Ghoshal , Erasmus Huber , Herbert Von Der Eltz , Sigrun Metz , Peter Kern
发明人： Gerald Sigler , Raymond Hui , Ina Deras , Richard Root , Mitali Ghoshal , Erasmus Huber , Herbert Von Der Eltz , Sigrun Metz , Peter Kern
IPC分类号： A61K31/47
摘要： Activated haptens useful for generating immunogens to HIV protease inhibitors, immunogens useful for producing antibodies to HIV protease inhibitors, and antibodies and labeled conjugates useful in immunoassays for the HIV protease inhibitor saquinavir. The novel haptens feature an activated functionality at the central, non-terminal hydroxyl group. Also described are monoclonal antibodies specific for saquinavir having less than 10% cross-reactivity with lopinavir, nelfinavir, amprenavir, ritonavir, and indinavir, and a murine hybridoma producing said antibodies.
摘要翻译： 可用于产生HIV蛋白酶抑制剂的免疫原的活化的半抗原，可用于产生HIV蛋白酶抑制剂的抗体的免疫原，以及可用于HIV蛋白酶抑制剂沙奎那韦的免疫测定的抗体和标记的缀合物。 新型半抗原在中心非末端羟基处具有活化的功能。 还描述了与洛匹那韦，奈非那韦，安仁那韦，利托那韦和茚地那韦具有小于10％交叉反应性的沙奎那韦特异性单克隆抗体，以及产生所述抗体的鼠杂交瘤。
-
公开(公告)号：US20070111234A1
公开(公告)日：2007-05-17
申请号：US11514777
申请日：2006-09-01
CPC分类号： C12Q1/6806 , C12Q2565/627 , C12Q2561/113
摘要： The present invention provides a method, a kit and a system for the analysis of samples with respect to the presence of biological DNA based on real-time polymerase chain reaction (PCR) in combination with an analyzing step. The method comprises the steps of performing a universal real-time PCR to amplify biological DNA in the sample, and analyzing the amplified biological DNA using a mass spectrometer (MS).
摘要翻译： 本发明提供了一种与分析步骤相结合的基于实时聚合酶链式反应（PCR）的生物DNA存在的样品分析方法，试剂盒和系统。 该方法包括以下步骤：进行通用实时PCR以扩增样品中的生物DNA，并使用质谱仪（MS）分析扩增的生物DNA。
-
-
-
-
-
-
-
-
-
搜索结果
87 条记录 (耗时 0.035 秒)
