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公开(公告)号：US5780234A
公开(公告)日：1998-07-14
申请号：US709263
申请日：1996-09-06
IPC分类号： C12N15/09 , C07H21/00 , C07H23/00 , C12Q1/68 , G01N33/566 , C07H21/02 , C07H21/04 , C12Q1/70
CPC分类号： C07H23/00 , C07H21/00 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827
摘要： The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
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公开(公告)号：US08441633B2
公开(公告)日：2013-05-14
申请号：US12915921
申请日：2010-10-29
申请人： Thai V. Truong , John M. Choi , Scott E. Fraser , Willy Supatto , David S. Koos
发明人： Thai V. Truong , John M. Choi , Scott E. Fraser , Willy Supatto , David S. Koos
IPC分类号： G01J3/40 , G01J3/30 , G01J1/58 , F21V9/16 , G01T1/10 , G21H3/02 , G21K5/00 , H01J65/06 , H01J65/08
CPC分类号： G02B21/16 , G01N21/6408 , G01N21/6428 , G01N21/6458 , G01N21/6486 , G01N2021/655 , G02B21/002 , G02B21/367
摘要： An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal illumination of light sheet microscopy are provided. With live imaging of whole Drosophila and zebrafish embryos, the high performance of MP-LISH compared to current state-of-the-art imaging techniques in maintaining good signal and high spatial resolution deep inside biological tissues (two times deeper than one-photon light sheet microscopy), in acquisition speed (more than one order of magnitude faster than conventional two-photon laser scanning microscopy), and in low phototoxicity are demonstrated. The inherent multi-modality of this new imaging technique is also demonstrated second harmonic generation light sheet microscopy to detect collagen in mouse tail tissue. Together, these properties create the potential for a wide range of applications for MP-LISH in 4D imaging of live biological systems.
摘要翻译： 提供了一种多光子激光荧光与光谱显微镜正交照明相结合的多光子光谱显微镜（MP-LISH）的设备和方法。 通过对整个果蝇和斑马鱼胚胎的实时成像，MP-LISH的高性能与目前最先进的成像技术相比，保持生物组织内部的良好信号和高空间分辨率（比单光子光更深两倍） 片状显微镜），在采集速度（比常规双光子激光扫描显微镜快超过一个数量级），并且具有低的光毒性。 这种新的成像技术的固有多模态也被证明了二次谐波发生光片显微镜检测小鼠尾部组织中的胶原蛋白。 共同的，这些属性为MP-LISH在活体生物系统4D成像中的应用提供了广泛的应用。
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公开(公告)号：US20120190835A1
公开(公告)日：2012-07-26
申请号：US13363022
申请日：2012-01-31
申请人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
发明人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
CPC分类号： C12Q1/682 , C12Q1/6818 , C12Q1/6841 , Y10T436/143333 , C12Q2563/107 , C12Q2537/149 , C12Q2525/301
摘要： The present invention relates to the use of fluorescently labeled nucleic acid probes to identify and image analytes in a biological sample. In the preferred embodiments, a probe is provided that comprises a target region able to specifically bind an analyte of interest and an initiator region that is able to initiate polymerization of nucleic acid monomers. After contacting a sample with the probe, labeled monomers are provided that form a tethered polymer. Triggered probes and self-quenching monomers can be used to provide active background suppression.
摘要翻译： 本发明涉及使用荧光标记的核酸探针来鉴定和成像生物样品中的分析物。 在优选的实施方案中，提供了包含能够特异性结合目标分析物的靶区域和能够引发核酸单体聚合的引发区域的探针。 在将样品与探针接触后，提供形成系留聚合物的标记单体。 触发的探针和自熄单体可用于提供活性背景抑制。
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公开(公告)号：US20120141981A1
公开(公告)日：2012-06-07
申请号：US11944264
申请日：2007-11-21
申请人： Periklis Pantazis , Ye Pu , Demetri Psaltis , John H. Hong , Scott E. Fraser
发明人： Periklis Pantazis , Ye Pu , Demetri Psaltis , John H. Hong , Scott E. Fraser
CPC分类号： G01N33/483 , A61B5/0075 , A61K49/0002 , B82Y5/00 , B82Y15/00 , B82Y20/00 , G01N21/636 , G02F1/37 , G06K9/00134
摘要： Second harmonic nanoprobes for imaging biological samples and a method of using such probes to monitor the dynamics of biological process using a field resonance enhanced second harmonic (FRESH) technique are provided. The second harmonic generating (SHG) nanoprobes are comprised of various kinds of nanocrystals that do not possess an inversion symmetry and therefore are capable of generating second harmonic signals that can then be detected by conventional two-photon microscopy for in vivo imaging of biological processes and structures such as cell signaling, neuroimaging, protein conformation probing, DNA conformation probing, gene transcription, virus infection and replication in cells, protein dynamics, tumor imaging and cancer therapy evaluation and diagnosis as well as quantification in optical imaging.
摘要翻译： 提供了用于成像生物样品的二次谐波纳米探针和使用这种探针来监测使用场共振增强二次谐波（FRESH）技术的生物过程动力学的方法。 二次谐波产生（SHG）纳米探针由不具有反转对称性的各种纳米晶体组成，因此能够产生二次谐波信号，然后可以通过传统的双光子显微镜检测生物过程的体内成像， 结构如细胞信号传导，神经成像，蛋白质构象探索，DNA构象探索，基因转录，细胞中的病毒感染和复制，蛋白质动力学，肿瘤成像和癌症治疗评估和诊断以及光学成像中的定量。
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公开(公告)号：US08107081B2
公开(公告)日：2012-01-31
申请号：US12243580
申请日：2008-10-01
IPC分类号： G01N21/00
CPC分类号： G01N21/7746 , G01N21/783 , G01N33/0037 , G01N33/0047 , G01N33/0049 , G01N33/0054 , G01N33/22 , G01N2021/773 , G01N2021/7789 , Y02A50/245 , Y02A50/246 , Y02A50/25
摘要： Micro-cavity gas or vapor sensors and gas or vapor detection methods. Optical energy is introduced into a resonant micro-cavity having a deformable coating such as a polymer. The coating swells or expands when it is exposed to or absorbs a gas or vapor, thereby changing the resonant wavelength of optical energy circulating within the micro-cavity/coating. Expansion or swelling of the coating may be reversible such that it contracts when gas or vapor diffuses from the coating. The coating deformation and/or a change of one or more optical properties of the optical energy circulating within the micro-cavity are used to detect the presence of the gas or vapor or molecules or particulates thereof.
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公开(公告)号：US07989198B2
公开(公告)日：2011-08-02
申请号：US12216062
申请日：2008-06-27
IPC分类号： C12M3/00
CPC分类号： G01N29/036 , B82Y5/00 , B82Y35/00 , G01N33/54373 , G01N2291/0257
摘要： A biofunctionalized nanoelectromechanical device (BioNEMS) for sensing single-molecules in solution by measuring the variation in the mechanical displacement of the BioNEMS device during a binding event is provided. The biofunctionalized nanoelectromechanical device according to the invention generally comprises a nanomechanical mechanical resonator, a detector integral with the mechanical resonator for measuring the mechanical displacement of the resonator, and electronics connected to the detector for communicating the results to a user. A system of biofunctionalized nanoelectromechanical devices and a method for utilizing the biofunctionalized nanoelectromechanical device of the present invention are also provided.
摘要翻译： 提供了一种生物功能化的纳米机电装置（BioNEMS），用于通过在绑定事件期间测量BioNEMS装置的机械位移的变化来感测溶液中的单分子。 根据本发明的生物功能化纳米机电装置通常包括纳米机械机械谐振器，与用于测量谐振器的机械位移的机械谐振器集成的检测器，以及连接到检测器的电子装置，用于将结果传达给用户。 还提供了一种生物功能化的纳米机电装置系统和利用本发明的生物功能化纳米机电装置的方法。
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公开(公告)号：US07727721B2
公开(公告)日：2010-06-01
申请号：US11371346
申请日：2006-03-07
申请人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
发明人： Niles A. Pierce , Robert Dirks , Scott E. Fraser
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6818 , C12Q1/6841 , Y10T436/143333 , C12Q2563/107 , C12Q2537/149 , C12Q2525/301
摘要： The present invention relates to the use of fluorescently labeled nucleic acid probes to identify and image analytes in a biological sample. In the preferred embodiments, a probe is provided that comprises a target region able to specifically bind an analyte of interest and an initiator region that is able to initiate polymerization of nucleic acid monomers. After contacting a sample with the probe, labeled monomers are provided that form a tethered polymer. Triggered probes and self-quenching monomers can be used to provide active background suppression.
摘要翻译： 本发明涉及使用荧光标记的核酸探针来鉴定和成像生物样品中的分析物。 在优选的实施方案中，提供了包含能够特异性结合目标分析物的靶区域和能够引发核酸单体聚合的引发区域的探针。 在将样品与探针接触后，提供形成系留聚合物的标记单体。 触发的探针和自熄单体可用于提供活性背景抑制。
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公开(公告)号：US20100085573A1
公开(公告)日：2010-04-08
申请号：US12573009
申请日：2009-10-02
申请人： Tao Lu , Tsu-Te Judith Su , Kerry J. Vahala , Scott E. Fraser
发明人： Tao Lu , Tsu-Te Judith Su , Kerry J. Vahala , Scott E. Fraser
IPC分类号： G01B9/02
CPC分类号： G01N21/7746
摘要： Resonant sensors and molecule detection methods utilizing split frequency. Optical energy is introduced into a microcavity, such as a toroid-shaped or spherical microcavity. A portion of the optical energy is backscattered and interacts with the introduced optical energy to form first and second modes of optical energy at respective first and second frequencies, also referred to as split frequency or mode doublets. One or more molecules bind to an outer surface of the microcavity and interact with an evanescent field of optical energy resonating within the microcavity. Binding of one or more molecules to the outer surface is detected based at least in part upon a change of the split frequency relative to a baseline split frequency.
摘要翻译： 谐振传感器和分子检测方法利用分频。 将光能引入微腔中，例如环形或球形微腔。 光能的一部分被反向散射并与引入的光能相互作用以在相应的第一和第二频率处形成第一和第二光能模式，也被称为分裂频率或模式双峰。 一个或多个分子结合到微腔的外表面并与在微腔内共振的光能的消逝场相互作用。 至少部分地基于分割频率相对于基线分割频率的变化来检测一个或多个分子与外表面的结合。
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59.发明申请公开(公告)号：US20090041316A1
公开(公告)日：2009-02-12
申请号：US12188076
申请日：2008-08-07
申请人： David S. Koos , Scott E. Fraser
发明人： David S. Koos , Scott E. Fraser
IPC分类号： G06K9/00
CPC分类号： G06K9/4638 , G02B21/367 , G06K9/00134
摘要： An system and method provide the ability to image a biological sample. A sample is embed to a support matrix that is compatible with an aqueous nature of the sample. A vibrating tissue sectioning system is coupled to a microscope and is used to remove a region of the sample without moving the sample. The sectioning of the sample occurs under a surface of an aqueous buffer in a basin. A positioning system enables the microscope to image adjacent sub-regions of the sample. The microscope image multiple sections of the sample in adjacent subregions using the vibrating tissue sectioning system and the positioning system.
摘要翻译： 系统和方法提供了对生物样品进行成像的能力。 将样品嵌入到与样品的水性质相容的载体基质中。 振动的组织切片系统耦合到显微镜并且用于去除样品的区域而不移动样品。 样品的切片发生在盆中的水性缓冲液的表面下。 定位系统使显微镜能够对样品的相邻子区域进行成像。 显微镜图像使用振动组织切片系统和定位系统在相邻次区域中的样品的多个部分。
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公开(公告)号：US20080284430A1
公开(公告)日：2008-11-20
申请号：US12109174
申请日：2008-04-24
IPC分类号： G01V3/00
CPC分类号： G01R33/302 , G01R33/31 , G01R33/341 , G01R33/3815 , G01R33/385 , G01R33/4808 , G01R33/5604
摘要： A magnetic resonance (MR) microscope and a dual-mode optic and MR microscope system are disclosed, The MR microscope is provided with a horizontal stage above which a sample to be analyzed is located, a radiofrequency coil assembly located above the horizontal stage and below the sample, a magnetic field gradient module located under the horizontal stage, and a heat exchange unit thermally coupled to the magnetic field gradient module and located under the magnetic field gradient module. The dual-mode optic and MR microscope system is provided with an MR microscope and an optical microscope, the optical microscope comprising a mirror located above the sample
摘要翻译： 公开了一种磁共振（MR）显微镜和双模光学和MR显微镜系统。该MR显微镜具有水平平台，在该水平平台上，待分析的样品位于其上，位于水平平台及以下的射频线圈组件 样品，位于水平台下方的磁场梯度模块，以及热耦合到磁场梯度模块并位于磁场梯度模块下方的热交换单元。 双模光学和MR显微镜系统配备有MR显微镜和光学显微镜，光学显微镜包括位于样品上方的反射镜
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