Methods, systems and compositions for monitoring enzyme activity and applications thereof
    57.
    发明申请
    Methods, systems and compositions for monitoring enzyme activity and applications thereof 有权
    用于监测酶活性的方法,系统和组合物及其应用

    公开(公告)号:US20070231804A1

    公开(公告)日:2007-10-04

    申请号:US11394637

    申请日:2006-03-31

    CPC classification number: C12Q1/66 C12Q1/42 G01N33/573

    Abstract: Methods of monitoring enzyme mediated reactions, and particularly nucleic acid synthesis reactions such as pyrosequencing methods that employ enzymatic reporter systems. The methods and systems provide elevated signal levels as compared to conventional pyrosequencing processes, and/or mediate de-phasing of sequencing analyses employing pyrosequencing or other “sequencing by synthesis” methods.

    Abstract translation: 监测酶介导的反应的方法,特别是使用酶报告系统的核酸合成反应,如焦磷酸测序方法。 与常规焦磷酸测序方法相比,该方法和系统提供升高的信号水平,和/或介导使用焦磷酸测序或其他“合成测序”方法的测序分析的脱相。

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