公开(公告)号：US20070190597A1

公开(公告)日：2007-08-16

申请号：US11674136

申请日：2007-02-12

申请人： Brian AGNEW ,  Schuyler Corry ,  Kyle Gee

发明人： Brian AGNEW ,  Schuyler Corry ,  Kyle Gee

IPC分类号： C12P21/06 ,  C07K16/46

CPC分类号： C12P21/005 ,  A61K47/549 ,  A61K47/61 ,  C07K1/13 ,  C07K14/47 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C12Y302/01096 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/531 ,  G01N33/532 ,  G01N33/533 ,  G01N33/534 ,  G01N33/581 ,  G01N33/582 ,  G01N33/583 ,  Y10T436/17

摘要： The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.

摘要翻译： 本发明通常涉及在寡糖键上官能化蛋白质，特别是抗体的方法，通过修饰糖基化来人源化抗体的方法，以及与修饰的寡糖连接的新型抗体。 本发明还涉及可用于产生本发明抗体的试剂盒。

公开(公告)号：US20050244976A1

公开(公告)日：2005-11-03

申请号：US11063707

申请日：2005-02-22

申请人： Kyle Gee ,  Wayne Patton

发明人： Kyle Gee ,  Wayne Patton

IPC分类号： C07D403/06 ,  C09B23/06 ,  G01N33/00 ,  G01N33/533 ,  G01N33/58 ,  G01N33/68

CPC分类号： C09B23/06 ,  G01N33/6839 ,  G01N33/6842

摘要： The present invention relates to methods of detecting anionic proteins in a sample with fluorescent carbocyanine dye compounds. The invention also describes methods of simultaneously detecting anionic and non-anionic proteins in a sample with discrete fluorescent signals produced by carbocyanine dye compounds. The invention is of use in a variety of fields including immunology, diagnostics, molecular biology and fluorescence based assays.

摘要翻译： 本发明涉及用荧光碳菁染料化合物检测样品中的阴离子蛋白质的方法。 本发明还描述了用碳菁染料化合物产生的离散荧光信号同时检测样品中阴离子和非阴离子蛋白的方法。 本发明可用于各种领域，包括免疫学，诊断学，分子生物学和基于荧光的测定。

公开(公告)号：US08846925B2

公开(公告)日：2014-09-30

申请号：US13471047

申请日：2012-05-14

申请人： Zhenjun Diwu ,  Jixiang Liu ,  Kyle Gee

发明人： Zhenjun Diwu ,  Jixiang Liu ,  Kyle Gee

IPC分类号： C07D471/04 ,  C07D491/14 ,  C07D495/14 ,  C07D495/04 ,  C07D491/04 ,  G01N33/58

CPC分类号： G01N33/582 ,  C07D471/04 ,  C07D491/04 ,  C07D491/044 ,  C07D491/14 ,  C07D491/147 ,  C07D491/22 ,  C07D495/04 ,  C07D495/14 ,  C07D495/22 ,  C07D498/14 ,  C12Q1/6816 ,  Y10S436/80 ,  Y10T436/141111 ,  Y10T436/143333 ,  Y10T436/145555 ,  C12Q2563/107

摘要： The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.

摘要翻译： 本发明描述了新颖的染料，包括掺入另外的稠合芳环的香豆素，罗丹明和罗丹醇。 本发明的染料比不具有稠合芳环的结构相似的染料长的波长吸收。 本发明的许多染料是有用的荧光染料。 本发明包括化学活性染料，染料共轭物，以及这些染料在染色样品和检测配体或其他分析物中的用途。

公开(公告)号：US20130323770A1

公开(公告)日：2013-12-05

申请号：US13985559

申请日：2012-02-14

申请人： Uma Lakshmipathy ,  Upinder Singh ,  Scott Grecian ,  Rene Quintanilla ,  Kyle Gee ,  Mahendra Rao

发明人： Uma Lakshmipathy ,  Upinder Singh ,  Scott Grecian ,  Rene Quintanilla ,  Kyle Gee ,  Mahendra Rao

IPC分类号： C12Q1/42

CPC分类号： G01N33/5005 ,  C09B11/24 ,  C09B19/00 ,  C09B21/00 ,  C09B57/00 ,  C09B57/02 ,  C12Q1/42 ,  C12Y301/03001 ,  G01N1/30 ,  G01N33/5073 ,  G01N33/581 ,  G01N33/582 ,  G01N33/586 ,  G01N2001/302 ,  G01N2333/916

摘要： The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.

摘要翻译： 本发明涉及用于染色活的干细胞的新的底物和方法。 染色可用于在体细胞重编程过程中鉴定诱导的多能干细胞集落。

公开(公告)号：US08124368B2

公开(公告)日：2012-02-28

申请号：US11774554

申请日：2007-07-06

申请人： Kyle Gee

发明人： Kyle Gee

IPC分类号： C12Q1/48 ,  C12Q1/50 ,  C12M1/34 ,  C12N9/48 ,  G01N27/327 ,  C12Q1/25 ,  C12Q1/00 ,  C12M1/00 ,  C12N9/10 ,  G01N27/411

CPC分类号： C12Q1/485 ,  G01N33/573

摘要： The present invention relates to kinase sensors comprising a metal chelator and a fluorophore, where the chelator comprises a quinoline group and where the fluorophore is conjugated to the chelator. The invention also relates to methods of using these kinase sensors as well as kits comprising the kinase sensors.

摘要翻译： 本发明涉及包含金属螯合剂和荧光团的激酶传感器，其中螯合剂包含喹啉基团，其中荧光团与螯合剂结合。 本发明还涉及使用这些激酶传感器的方法以及包含激酶传感器的试剂盒。

公开(公告)号：US20100009381A1

公开(公告)日：2010-01-14

申请号：US12500854

申请日：2009-07-10

申请人： Brian Agnew ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Thomas Steinberg ,  Wayne Patton

发明人： Brian Agnew ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Thomas Steinberg ,  Wayne Patton

IPC分类号： G01N33/53

CPC分类号： G01N33/5008 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/502 ,  G01N33/5091 ,  G01N2333/4709 ,  Y10T436/142222 ,  Y10T436/145555 ,  Y10T436/147777 ,  Y10T436/201666

摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

摘要翻译： 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

公开(公告)号：US20080009026A1

公开(公告)日：2008-01-10

申请号：US11774554

申请日：2007-07-06

申请人： Kyle Gee

发明人： Kyle Gee

IPC分类号： C12Q1/48

CPC分类号： C12Q1/485 ,  G01N33/573

摘要： The present invention relates to kinase sensors comprising a metal chelator and a fluorophore, where the chelator comprises a quinoline group and where the fluorophore is conjugated to the chelator. The invention also relates to methods of using these kinase sensors as well as kits comprising the kinase sensors.

摘要翻译： 本发明涉及包含金属螯合剂和荧光团的激酶传感器，其中螯合剂包含喹啉基团，其中荧光团与螯合剂结合。 本发明还涉及使用这些激酶传感器的方法以及包含激酶传感器的试剂盒。

公开(公告)号：US20070093652A1

公开(公告)日：2007-04-26

申请号：US11560579

申请日：2006-11-16

申请人： Zhenjun Diwu ,  Jixiang Liu ,  Kyle Gee

发明人： Zhenjun Diwu ,  Jixiang Liu ,  Kyle Gee

IPC分类号： C07K14/47 ,  C07H21/04 ,  C07D491/14

CPC分类号： G01N33/582 ,  C07D471/04 ,  C07D491/04 ,  C07D491/044 ,  C07D491/14 ,  C07D491/147 ,  C07D491/22 ,  C07D495/04 ,  C07D495/14 ,  C07D495/22 ,  C07D498/14 ,  C12Q1/6816 ,  Y10S436/80 ,  Y10T436/141111 ,  Y10T436/143333 ,  Y10T436/145555 ,  C12Q2563/107

摘要： The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.

摘要翻译： 本发明描述了新颖的染料，包括掺入另外的稠合芳环的香豆素，罗丹明和罗丹醇。 本发明的染料比不具有稠合芳环的结构相似的染料长的波长吸收。 本发明的许多染料是有用的荧光染料。 本发明包括化学活性染料，染料共轭物，以及这些染料在染色样品和检测配体或其他分析物中的用途。

公开(公告)号：US20060141554A1

公开(公告)日：2006-06-29

申请号：US10966536

申请日：2004-10-14

申请人： Kyle Gee ,  Courtenay Hart ,  Richard Haugland ,  Wayne Patton ,  Scott Whitney

发明人： Kyle Gee ,  Courtenay Hart ,  Richard Haugland ,  Wayne Patton ,  Scott Whitney

IPC分类号： G01N33/48 ,  C07F15/04

CPC分类号： C07D263/62 ,  A61K49/0021 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/0052 ,  C07D311/12 ,  C07D311/16 ,  C07D311/18 ,  C07D405/14 ,  C07F5/022 ,  G01N1/30 ,  G01N33/533 ,  G01N33/582

摘要： The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

公开(公告)号：US20060024833A1

公开(公告)日：2006-02-02

申请号：US11191799

申请日：2005-07-27

申请人： Kyle Gee ,  Vladimir Martin

发明人： Kyle Gee ,  Vladimir Martin

IPC分类号： G01N33/20

CPC分类号： G01N33/84 ,  C07C229/18 ,  C07C311/44 ,  C07D209/14 ,  C07D221/14 ,  C07D265/38 ,  C07D311/82 ,  C07D311/90 ,  C07D413/04 ,  C07K14/195 ,  C07K14/43595 ,  C09B5/60 ,  C09B5/62 ,  C09B11/06 ,  C09B11/08 ,  C09B19/00 ,  C09B19/005 ,  C09B57/08 ,  C09K11/06 ,  C09K2211/1014 ,  G01N33/20 ,  G01N2458/00

摘要： The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.

摘要翻译： 本发明提供用于检测靶金属离子的荧光化合物，其中化合物表现出大于50nm的斯托克斯位移，并且可检测信号通过光诱导电子转移（PET）调节。 本发明化合物由三种功能元件组成，即离子感测部分（螯合部分），报道部分（荧光团或荧光蛋白）以及本发明化合物的感测和报道部分之间的间隔物或接头，其允许在结合金属时的PET 通过适当的波长离子和激发。