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190 条记录 (耗时 0.038 秒)

    Bio-acceptable conduits and method providing the same
    52.
    发明申请
    Bio-acceptable conduits and method providing the same 审中-公开
    生物可接受的管道和提供它的方法

    公开(公告)号：US20100047310A1

    公开(公告)日：2010-02-25

    申请号：US12458526

    申请日：2009-07-15

    申请人： Chien-Chung CHEN ,  Jeng-Chang YANG ,  Suhan LI ,  En-Sheng KE ,  Yung-Sheng LIN

    发明人： Chien-Chung CHEN ,  Jeng-Chang YANG ,  Suhan LI ,  En-Sheng KE ,  Yung-Sheng LIN

    IPC分类号： A61F2/04 ,  C12N5/06 ,  A61F2/02 ,  B29C47/00

    CPC分类号： A61L27/58 ,  A61B17/1128 ,  A61L27/383 ,  A61L27/3878 ,  A61L27/48 ,  A61L2430/32 ,  D01D5/0069 ,  D01D5/24

    摘要： Disclosed is a bio-electrospinning technique for preparing a cell-containing, oriented, continuous tubular scaffold, made of biodegradable polymer, designed for use as a nerve guide conduit (NGC) in nerve regeneration. With a coaxial spinneret, the PC-12 cell medium solution was co-electrospun into a core of tubular fibers, with PLA on the outer shell. The resulted fibers' morphology was characterized via SEM and optical microscopy, and following structural characteristics were found: 1. the larger, hollow fibers had diameters in tenth of microns and wall thicknesses around few microns, 2. an orientation in a preferred direction with the aid of a high-rotating collection device. The fluorescent PC12 cells embedded within the scaffold were cultured and nerve growth factor was added. We observed cells could not only survive the process, but also sustain their viability by undergoing differentiation process, extending neurite along the micro tubular scaffold in the desired direction. All these results demonstrate its potential application for advanced NGC.

    摘要翻译： 公开了一种生物电纺丝技术，用于制备由可生物降解的聚合物制成的含细胞定向的连续管状支架，设计用于神经再生中的神经导管（NGC）。 使用同轴喷丝头，将PC-12细胞培养基溶液共同电纺丝成管状纤维的核心，PLA在外壳上。 通过SEM和光学显微镜对所得纤维的形态进行了表征，并发现了以下结构特征：1.较大的中空纤维的直径为十分之一微米，壁厚约为几微米，2.优选方向的取向为 辅助高旋转收集装置。 培养嵌入支架内的荧光PC12细胞，加入神经生长因子。 我们观察到细胞不仅可以在该过程中存活，而且通过进行分化过程维持其活力，沿着所需方向沿着微管状支架延伸神经突。 所有这些结果表明其潜在应用于先进的NGC。

    Cell-free tissue replacement for tissue engineering
    53.
    发明授权
    Cell-free tissue replacement for tissue engineering 有权
    组织工程无细胞组织置换

    公开(公告)号：US07402319B2

    公开(公告)日：2008-07-22

    申请号：US10672689

    申请日：2003-09-26

    申请人： Christine Schmidt ,  Terry Hudson

    发明人： Christine Schmidt ,  Terry Hudson

    IPC分类号： A61F2/02

    CPC分类号： A61L27/3633 ,  A61L27/3604 ,  A61L27/3675 ,  A61L27/3687 ,  A61L27/383 ,  A61L27/3878 ,  A61L2430/40

    摘要： The present invention is a natural, cell-free tissue replacement that does not require difficult or extensive preparation made by washing tissue replacement in a solution including one or more sulfobetaines and an anionic surface-active detergent and washing the tissue replacement in serial solutions of the buffered salt to remove excess detergent. The natural, cell-free tissue replacement may be a nerve graft that supports axonal regeneration, guides the axons toward the distal nerve end and/or is immunologically tolerated. Other forms of the invention are a composition and kit prepared by the method of making a native, cell-free tissue replacement. The present invention may be modified for use in diagnostic, therapeutic, and prophylactic applications.

    摘要翻译： 本发明是一种天然的无细胞组织替代物，其不需要通过在包括一种或多种磺基甜菜碱和阴离子表面活性洗涤剂的溶液中洗涤组织置换而制备的困难或广泛的制备，并且洗涤组织替换物 缓冲盐以除去多余的洗涤剂。 天然的无细胞组织置换可以是支持轴突再生的神经移植物，将轴突引导到远端神经末端和/或免疫耐受。 本发明的其它形式是通过制备天然的，无细胞的组织置换的方法制备的组合物和试剂盒。 本发明可以被修改用于诊断，治疗和预防应用。

    Methods and Composition for Transplantation of Dopaminergic Neurons for Parkinson's Disease
    54.
    发明申请
    Methods and Composition for Transplantation of Dopaminergic Neurons for Parkinson's Disease 审中-公开
    用于帕金森病多巴胺能神经元移植的方法和组成

    公开(公告)号：US20070274962A1

    公开(公告)日：2007-11-29

    申请号：US10580547

    申请日：2004-12-02

    申请人： Ge Lui

    发明人： Ge Lui

    IPC分类号： A61K35/12 ,  A61P25/16

    CPC分类号： A61L27/383 ,  A61K9/0085 ,  A61K35/30 ,  A61L27/3878 ,  A61L27/3895 ,  A61L27/58

    摘要： This invention discloses methods to attach and grow a monolayer of cultured human retinal pigment epithelial cells (RPE) for use in implantation into the brain as a treatment for Parkinson's disease. The invention will enable the delivery of the transplanted RPE cells in microcarriers composed of intergratable or degradable substrates, including glass, plastic, polymer gels, gelatin and collagen, and glycosaminoglycans (GAGS). The invention involves the coating of the microcarrier surface with diamond-like carbon, alone or in combination with attachment factors such as laminin, fibronectin, RGDS, and extracellular matrix to increase the attachment of the RPE to the surface. Additionally the invention discloses the use of bFGF conjugated with polycarbophil, EGF conjugated with polycarbophil and heparin sulfate as also being incorporated into the micro carrier to augment attachment and proliferation of RPE during transplantation.

    摘要翻译： 本发明公开了将培养的人视网膜色素上皮细胞（RPE）的单层附着和生长用于植入脑中作为帕金森病治疗的方法。 本发明将能够将包含玻璃，塑料，聚合物凝胶，明胶和胶原以及糖胺聚糖（GAGS）的可耐受或可降解的基材组成的微载体中的移植的RPE细胞递送。 本发明涉及用类金刚石碳单独或与附着因子如层粘连蛋白，纤连蛋白，RGDS和细胞外基质组合来涂覆微载体表面，以增加RPE与表面的连接。 此外，本发明公开了与聚卡波非缀合的bFGF，与聚卡波非和硫酸肝素结合的EGF也被并入微载体中以增加移植期间RPE的附着和增殖的用途。

    Composition and Methods for Cell Culturing and Tissue Culture Platforms
    55.
    发明申请
    Composition and Methods for Cell Culturing and Tissue Culture Platforms 有权
    细胞培养和组织培养平台的组成和方法

    公开(公告)号：US20070274961A1

    公开(公告)日：2007-11-29

    申请号：US10575247

    申请日：2004-10-08

    申请人： Ge Lui

    发明人： Ge Lui

    IPC分类号： A61K35/12 ,  A61P25/00 ,  B05D7/22 ,  C12M1/34 ,  C12M3/04 ,  C12N1/04 ,  C12N1/38 ,  C12N5/02

    CPC分类号： A61L27/3878 ,  A61L27/303 ,  A61L27/383 ,  C12M25/14 ,  C12N5/0068 ,  C12N5/0621 ,  C12N2502/28 ,  C12N2533/10 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/54

    摘要： This present invention describes a method of coating a polymer surface with diamond-like carbon (DLC) to render it useful as a carrier for cells derived from neural crest origin, preferable neuronal cells that form dendrites. The biopolymer to be coated with the DLC will include biodegradable polymers and other implantable biopolymers to act as a carrier system for cell transplantation into the various parts of the body, including the brain, the eye, the central and peripheral nervous system, the lung, the liver, the spleen, the kidney, and the bone and cartilage. The biopolymer can be in sheet form or microparticle form, and can be imbedded with, or incorporated into during its synthesis, attachment or growth promoting reagents to enhance and support neuraonal call attachment and growth. This coating method can also augment other coating agents such as extracellular matrix (ECM) secreted by cultured bovine corneal endothelial cells, as well as adhesive molecules such as fibronectin, laminin, and RGDS. The coating step can be a sequential process where the DLC layer will be aded on top of an ECM coated surface or an attachment factor coated surface.

    摘要翻译： 本发明描述了用金刚石样碳（DLC）涂覆聚合物表面的方法，以使其可用作来自神经嵴起源的细胞的载体，优选的形成树突的神经元细胞。 要涂覆DLC的生物聚合物将包括可生物降解的聚合物和其他可植入的生物聚合物，用作细胞移植到身体各个部位的载体系统，包括脑，眼睛，中枢和周围神经系统，肺， 肝，脾，肾，骨和软骨。 生物聚合物可以是片状或微粒形式，并且可以在其合成中嵌入或并入其中，附着或生长促进试剂以增强和支持神经调节附着和生长。 该涂布方法还可以增加其它包被剂，例如由培养的牛角膜内皮细胞分泌的细胞外基质（ECM），以及粘附分子如纤连蛋白，层粘连蛋白和RGDS。 涂覆步骤可以是顺序过程，其中DLC层将被涂覆在ECM涂覆的表面或附着因子涂覆的表面的顶部上。

    Nano- and micro-scale engineering of polymeric scaffolds for vascular tissue engineering
    56.
    发明申请
    Nano- and micro-scale engineering of polymeric scaffolds for vascular tissue engineering 审中-公开
    用于血管组织工程的聚合物支架的纳米和微尺度工程

    公开(公告)号：US20060085063A1

    公开(公告)日：2006-04-20

    申请号：US11252229

    申请日：2005-10-17

    申请人： V. Shastri ,  Jay Sy

    发明人： V. Shastri ,  Jay Sy

    IPC分类号： A61F2/06

    CPC分类号： A61L31/005 ,  A61F2/0077 ,  A61F2/02 ,  A61F2/06 ,  A61F2/82 ,  A61K47/6957 ,  A61L27/18 ,  A61L27/34 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3826 ,  A61L27/383 ,  A61L27/3878 ,  A61L27/3886 ,  A61L27/507 ,  A61L31/06 ,  A61L31/10 ,  A61L2430/32 ,  B82Y5/00 ,  C12N5/0691 ,  C12N2535/10 ,  C08L75/04 ,  C08L67/04 ,  C08L89/00

    摘要： In one aspect, the invention relates to a synthetic conduit comprising a substantially tubular body comprising substantially circumferential polymer fibers; wherein the body has an exterior surface, an interior surface, and a lumen extending therethrough; and wherein the body has microscale features disposed at the interior surface. In a further aspect, cells can be optionally adhered to the exterior and/or interior surface of the substantially tubular body. In various aspects, the conduit can be used as a vascular prosthesis, a stent, or a nerve regeneration scaffold. Methods of preparing and implanting same are also provided. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

    摘要翻译： 一方面，本发明涉及一种合成管道，其包括基本上管状的主体，其包括基本上周向的聚合物纤维; 其中所述主体具有外表面，内表面和延伸穿过其中的内腔; 并且其中所述主体具有设置在所述内表面处的微细特征。 在另一方面，细胞可以任选地粘附到基本管状体的外部和/或内表面。 在各个方面，导管可用作血管假体，支架或神经再生支架。 还提供了制备和植入其的方法。 该摘要旨在作为用于在特定技术中进行搜索的扫描工具，而不意在限制本发明。

    Materials and methods for nerve grafting
    59.
    发明申请
    Materials and methods for nerve grafting 审中-公开
    神经移植的材料和方法

    公开(公告)号：US20030077258A1

    公开(公告)日：2003-04-24

    申请号：US10218315

    申请日：2002-08-13

    发明人： David F. Muir

    IPC分类号： A61K045/00 ,  A61K038/47 ,  C12N005/08

    CPC分类号： A61L27/3687 ,  A61K35/30 ,  A61K38/51 ,  A61L27/227 ,  A61L27/3604 ,  A61L27/3675 ,  A61L27/3683 ,  A61L27/383 ,  A61L27/3878 ,  A61L31/047 ,  A61L2430/32 ,  A61K2300/00

    摘要： The subject invention pertains to compositions and methods for promoting repair of damaged nerve tissue using nerve grafts and preparation of nerve grafts. The compositions and methods of the subject invention can be employed to restore the continuity of nerve interrupted by disease, traumatic events or surgical procedures. Compositions of the subject invention comprise one or more chondroitin sulfate proteoglycan (CSPG)-degrading enzymes that promote axonal penetration into damaged nerve tissue and nerve graft. The invention also concerns methods for promoting repair of damaged nerve tissue using the present compositions and nerve tissue treated according to such methods. The invention also includes storage solutions for nerve tissue.

    摘要翻译： 本发明涉及使用神经移植物促进修复损伤的神经组织并制备神经移植物的组合物和方法。 本发明的组合物和方法可以用于恢复由疾病，外伤事件或外科手术中断的神经的连续性。 本发明的组合物包含一种或多种硫酸软骨素蛋白聚糖（CSPG）降解酶，其促进轴突穿透进入损伤的神经组织和神经移植物。 本发明还涉及使用本发明组合物和根据这些方法治疗的神经组织促进受损神经组织修复的方法。 本发明还包括神经组织的储存解决方案。