公开(公告)号：US10004799B2

公开(公告)日：2018-06-26

申请号：US15447972

申请日：2017-03-02

申请人： Longhorn Vaccines and Diagnostics, LLC

发明人： Gerald W. Fischer ,  Luke T. Daum

IPC分类号： A61K39/145 ,  C07K14/33 ,  A61K39/12 ,  A61K39/04 ,  C07K14/35 ,  C07K16/10 ,  C07K16/12 ,  C12N7/00 ,  A61K39/00 ,  C07K14/005

CPC分类号： A61K39/145 ,  A61K39/04 ,  A61K39/12 ,  A61K2039/525 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/53 ,  A61K2039/543 ,  A61K2039/545 ,  A61K2039/55566 ,  A61K2039/575 ,  A61K2039/6037 ,  A61K2039/6043 ,  A61K2039/6068 ,  A61K2039/70 ,  C07K14/005 ,  C07K14/33 ,  C07K14/35 ,  C07K16/1018 ,  C07K16/1289 ,  C07K2319/40 ,  C07K2319/42 ,  C12N7/00 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2760/16161

摘要： The invention relates to composite antigens comprising a peptide with contiguous amino acid sequence derived from a plurality of antigenic epitopes of one or more pathogens that induces an immune response in a mammal that is protective against infection by the one or more pathogens. In addition, the invention relates to vaccines comprising composite antigens and to method for treating and preventing an infection.

公开(公告)号：US20180147266A1

公开(公告)日：2018-05-31

申请号：US15881135

申请日：2018-01-26

申请人： MERZ PHARMA GMBH & CO. KGAA

发明人： Michael PFEIL ,  Andreas WIESENBURG

IPC分类号： A61K38/48 ,  A61K39/08 ,  C12N9/52 ,  C12N1/20 ,  C12M1/12 ,  C12M1/34 ,  C07K14/33

CPC分类号： A61K38/4893 ,  A61K39/08 ,  C07K14/33 ,  C12M37/00 ,  C12M41/40 ,  C12N1/20 ,  C12N9/52 ,  C12Y304/24069 ,  Y02A50/469

摘要： The present invention relates to a process for preparing a highly pure neurotoxic component of a botulinum toxin by cultivating clostridium botulinum under conditions that allow production of a botulinum toxin, and isolating the neurotoxic component from the botulinum toxin. In addition, the present invention relates to a highly pure neurotoxic component of a botulinum toxin obtainable by the process of the present invention and uses thereof.

公开(公告)号：US09975929B2

公开(公告)日：2018-05-22

申请号：US15123408

申请日：2015-03-04

申请人： MERZ PHARMA GmbH & CO. KGaA

发明人： Jürgen Frevert ,  Fred Hofmann ,  Michael Schmidt

IPC分类号： C07K14/33 ,  A61K38/00

CPC分类号： C07K14/33 ,  A61K38/00 ,  C07K2319/00 ,  C12Y304/11013 ,  Y02A50/469

摘要： This invention relates to novel recombinant clostridial neurotoxins exhibiting increased duration of effect and to methods for the manufacture of such recombinant clostridial neurotoxins. These novel recombinant clostridial neurotoxins comprise a random coil domain, and the methods comprise the steps of inserting a nucleic acid sequence coding for a random coil domain into a nucleic acid sequence coding for a parental clostridial neurotoxin and expression of the recombinant nucleic acid sequence comprising the random coil domain-coding sequence in a host cell. The invention further relates to novel recombinant single-chain precursor clostridial neurotoxins used in such methods, nucleic acid sequences encoding such recombinant single-chain precursor clostridial neurotoxins, and pharmaceutical compositions comprising the recombinant clostridial neurotoxin with increased duration of effect.

公开(公告)号：US09957305B2

公开(公告)日：2018-05-01

申请号：US14344749

申请日：2012-09-13

申请人： Jimmy D. Ballard ,  Jordi M. Melton ,  Latisha Heinlen ,  Elaine E. Hamm

发明人： Jimmy D. Ballard ,  Jordi M. Melton ,  Latisha Heinlen ,  Elaine E. Hamm

IPC分类号： A61K38/00 ,  C07K14/33 ,  A61K39/08 ,  C07K16/12 ,  G01N33/569

CPC分类号： C07K14/33 ,  A61K39/08 ,  C07K16/1282 ,  G01N33/56911 ,  G01N2333/33 ,  G01N2469/20

摘要： Embodiments of the invention are directed to a composition comprising a recombinant protein in soluble form wherein said recombinant protein comprises a portion of the Clostridium difficile toxin B sequence that comprises an epitope for anti-toxin B antibody. Other embodiments of the invention are directed to the generation of antibodies using peptide fragments of C. difficile toxin B.

公开(公告)号：US09915647B2

公开(公告)日：2018-03-13

申请号：US15087521

申请日：2016-03-31

申请人： Baystate Medical Center, Inc. ,  UNIVERSITY OF MASSACHUSETTS

发明人： Nagendra Yadava ,  Alejandro Pablo Heuck ,  Chul Kim

IPC分类号： C12Q1/02 ,  G01N33/50 ,  C07K14/33

CPC分类号： G01N33/5079 ,  C07K14/33 ,  G01N33/5005 ,  G01N2333/33

摘要： The invention provides methods, compositions, devices, and kits relating to the use of cholesterol-dependent cytolysins (e.g., PFOs) for measuring intracellular mitochondrial activity.

公开(公告)号：US20180043032A1

公开(公告)日：2018-02-15

申请号：US15727860

申请日：2017-10-09

申请人： Allergan, Inc.

发明人： Jennifer L. Ton ,  Ronald C. Bates ,  Ji Zheng ,  Phillip P. Nguyen ,  Timothy E. O'Neill

IPC分类号： A61K47/64 ,  C07K14/33 ,  G01N27/447

CPC分类号： A61K47/6415 ,  A61K47/64 ,  C07K14/33 ,  G01N27/447

摘要： The present invention provides for a carrier complex for administration of therapeutic agents. In one aspect, an isolated C. botulinum carrier complex is provided, where the carrier complex lacks a native neurotoxin subunit.

公开(公告)号：US20170327811A1

公开(公告)日：2017-11-16

申请号：US15664456

申请日：2017-07-31

申请人： Allergan, Inc.

发明人： Jennifer L. Ton ,  Hemant A. Patel ,  Ronald C. Bates ,  Wajdie M. Ahmad

IPC分类号： C12N9/52 ,  B01D15/36 ,  C07K14/33

摘要： Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

公开(公告)号：US09777045B2

公开(公告)日：2017-10-03

申请号：US15205476

申请日：2016-07-08

申请人： Longhorn Vaccines and Diagnostics, LLC

发明人： Gerald W. Fischer ,  Luke T. Daum

IPC分类号： C07K14/005 ,  C07K14/33 ,  C07K14/35 ,  C07K7/06 ,  C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K39/102 ,  A61K39/09 ,  A61K39/00 ,  A61K39/085

CPC分类号： C07K14/005 ,  A61K39/085 ,  A61K39/092 ,  A61K39/102 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/525 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/543 ,  A61K2039/545 ,  A61K2039/55566 ,  A61K2039/575 ,  A61K2039/6037 ,  A61K2039/6043 ,  A61K2039/6068 ,  A61K2039/70 ,  C07K7/06 ,  C07K14/33 ,  C07K14/35 ,  C07K2319/40 ,  C07K2319/42 ,  C12N7/00 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2760/16161

摘要： Disclosed are immunogenic compositions and methods for their use in the formulation and administration of therapeutic and prophylactic pharmaceutical agents. In particular, the invention provides immunogenic compositions and methods for preventing, treating, and/or ameliorating the symptoms of one or more microbial infections, including, for example, influenza.

公开(公告)号：US09745354B2

公开(公告)日：2017-08-29

申请号：US14529147

申请日：2014-10-31

申请人： WYETH LLC

发明人： Mark E. Ruppen ,  Justin Keith Moran ,  Kathrin Ute Jansen ,  Annaliesa Sybil Anderson ,  Robert G. K. Donald ,  Narender K. Kalyan ,  Maninder K. Sidhu ,  Michael James Flint

IPC分类号： C12P1/00 ,  C07K14/33 ,  C07K16/12 ,  C12N15/74 ,  C12N9/10 ,  C12N9/99 ,  A61K39/08

CPC分类号： C07K14/33 ,  A61K39/08 ,  C07K16/1282 ,  C07K2317/33 ,  C07K2317/76 ,  C07K2317/92 ,  C12N9/1051 ,  C12N9/99 ,  C12N15/74

摘要： In one aspect, the invention relates to an immunogenic composition that includes a mutant Clostridium difficile toxin A and/or a mutant Clostridium difficile toxin B. Each mutant toxin includes a glucosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation, relative to the corresponding wild-type C. difficile toxin. The mutant toxins may further include at least one amino acid that is chemically crosslinked. In another aspect, the invention relates to antibodies or binding fragments thereof that binds to said immunogenic compositions. In further aspects, the invention relates to isolated nucleotide sequences that encode any of the foregoing, and methods of use of any of the foregoing compositions.

公开(公告)号：US20170239340A1

公开(公告)日：2017-08-24

申请号：US15421808

申请日：2017-02-01

申请人： Valneva Austria GmbH ,  Intercell USA, Inc.

发明人： LARRY R. ELLINGSWORTH ,  David Flyer ,  Jing-Hui Tian ,  Steven R. Fuhrmann ,  Stefanie Kluepfel-Stahl ,  Gregory M. Glenn ,  Kerstin Westritschnig

IPC分类号： A61K39/08 ,  C07K16/12 ,  C07K14/33

CPC分类号： A61K39/08 ,  A61K2039/53 ,  A61K2039/55505 ,  A61K2039/55544 ,  A61K2039/575 ,  C07K14/33 ,  C07K16/1282 ,  C07K2317/76 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/55

摘要： This present invention provides C-TAB.G5 and C-TAB.G5.1 isolated polypeptides comprising the receptor binding domains of C. difficile toxin A and toxin B as set forth in the amino acid sequences of SEQ ID NO: 2 and SEQ ID NO: 4. The C-TAB.G5 and C-TAB.G5.1 isolated polypeptides may be used to neutralize toxic effects of C. difficile toxin A and/or toxin B.