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65 条记录 (耗时 0.035 秒)

    Pyrroles substituted by oligonucleotides
    61.
    发明授权
    Pyrroles substituted by oligonucleotides 失效
    由寡核苷酸取代的吡咯

    公开(公告)号：US07446186B2

    公开(公告)日：2008-11-04

    申请号：US10536317

    申请日：2003-12-16

    申请人： Bernard Mandrand ,  Daniela Zsoldos ,  Alain Laurent ,  Carole Chaix ,  Nicolas Spinelli

    发明人： Bernard Mandrand ,  Daniela Zsoldos ,  Alain Laurent ,  Carole Chaix ,  Nicolas Spinelli

    IPC分类号： C07H21/00 ,  C12Q1/68

    CPC分类号： C07H21/00 ,  Y02P20/55

    摘要： The invention relates to novel pyrrole derivatives of the formula (I) which make it possible to immobilize and address oligonucleotides by electropolymerization. Said invention also relates to thus produced electroactive polymers and to methods for using them for detecting, identifying and dosing analytes in a sample. (I) wherein R1 is one type of oligonucleotide, Y is S or O, X is a spacer arm selected from —(CH2)—O—, —(CH2) P O—[(CH2)2—O]q—, —(CH2)r; —CO—NR′—(CH2)r—O—, —CH2r—NCH3—(CH2)r—O—, —CH2)rCO—NR′—[CH2)2—O]s—, —(CH2)rNCH3[(CH2)2—O] S —, R′ is H or CH3, n is an integer number ranging from 1 to 5, p is an integer number ranging from 1 to 2, q is an integer number ranging from 1 to 4, r is an integer number ranging from 1 to 3, r′ is an integer number ranging from 1 to 3, s is an integer number ranging from 1 to 3, n, p, q, r, r′ and s are identical or different, a pyrrole cycle is substituted in a position 2, 3, 4 or 5.

    摘要翻译： 本发明涉及式（I）的新型吡咯衍生物，其可以通过电聚合固定和解决寡核苷酸。 所述发明还涉及如此制备的电活性聚合物以及使用它们用于检测，鉴定和给药样品中分析物的方法。 （I）其中R 1是一种类型的寡核苷酸，Y是S或O，X是选自以下的间隔臂 - （CH 2）2 - ， - （ CH 2 O - [（CH 2）2 -O] q - ， - （CH 2）n - -CO-NR' - （CH 2）2 -O - ， - CH 2 R n -NCH 3 - （CH 2）n -O - ， - CH 2）n CO-NR' - [CH 2 - 2 - > - - - - - - - - - - - - - - - - （CH 2） [（CH 2）2 -O] -SS - ，R'是H或CH 3， 3 ，n为1〜5的整数，p为1〜2的整数，q为1〜4的整数，r为1〜3的整数， r'为1〜3的整数，s为1〜3的整数，n，p，q，r，r'和s相同或不同，吡咯环在2位， 3，4或5。

    Bi-functional metallocenes, preparation process and use in the labeling or biological molecules
    62.
    发明授权
    Bi-functional metallocenes, preparation process and use in the labeling or biological molecules 失效

    公开(公告)号：US07301041B2

    公开(公告)日：2007-11-27

    申请号：US10501347

    申请日：2003-02-14

    申请人： Carole Chaix-Bauvais ,  Corinne Moustrou ,  Aude-Emmanuelle Navarro ,  Hugues Brisset ,  Francis Garnier ,  Bernard Mandrand ,  Nicolas Spinelli

    发明人： Carole Chaix-Bauvais ,  Corinne Moustrou ,  Aude-Emmanuelle Navarro ,  Hugues Brisset ,  Francis Garnier ,  Bernard Mandrand ,  Nicolas Spinelli

    IPC分类号： C07F17/02 ,  C07K1/00

    CPC分类号： C07F17/02 ,  Y02P20/55

    摘要： The invention relates to bi-functionalised metallocenes of general formula (I) where Me=a transition metal, preferably chosen from Fe, Ru and Os, Y and Z, when identical are selected from —(CH2)n—O—, (CH2)—O—[(CH2)2—O]P— and —(CH2)q—CONH—(CH2)r—O—, or Y=—(CH2)S—NH— and Z=—(CH2)t—COO—, n=a whole number from 3 to 6 inclusive, p=a whole number from 1 to 4 inclusive, q=a whole number from 0 to 2 inclusive, r=a whole number from 0 to 2 inclusive, s=a whole number from 2 to 5 inclusive, t=a whole number from 3 to 6 inclusive, R and R′=H atoms or are protective groups used in oligonucleotide and peptide synthesis, where at least one of R or R′ is protective group used in oligonucleotide and peptide synthesis and R and R′ are as defined below: (i) when Z and Y are selected from (CH2)n—O—, —(CH2)—O—[(CH2)2—O]p— and —(CH2)q—CONH—(CH2)r—O—, then R and R′ are protective groups used in oligonucleotide synthesis and R is a group which can leave a free OH group after deprotection, preferably a photolabile group such as monomethroxythoxytrityl, dimethoxytrityl, t-butyldimethylsilyl, acetyl or trifluroacetyl, and R′ is a phosphorylated group which can react with a free OH, preferably a phosphodiester, phosphoramidite or H-phosphonate and (ii) when Y=—(CH2)s—NH— and Z=—(CH2)t—COO—, then R is a protective group used in the synthesis of peptides and is an amino-protecting group, preferably 9-fluorenyloxycarbonyl, t-butoxycarbonyl or benzyloxycarbonyl and R′=H. The above is applied in marking.

    Reagent and method for the detection of a nucleotide sequence with signal amplification
    65.
    发明授权
    Reagent and method for the detection of a nucleotide sequence with signal amplification 失效
    用于检测具有信号放大的核苷酸序列的试剂和方法

    公开(公告)号：US5695936A

    公开(公告)日：1997-12-09

    申请号：US433505

    申请日：1995-06-14

    申请人： Bernard Mandrand ,  Philippe Cros ,  Thierry Delair ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    发明人： Bernard Mandrand ,  Philippe Cros ,  Thierry Delair ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    IPC分类号： C08G81/02 ,  C12Q1/68

    CPC分类号： C12Q1/6813 ,  C08G81/02 ,  C12Q1/682

    摘要： Methods for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The methods comprise use of a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with a probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefor lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.

    摘要翻译： PCT No.PCT / FR94 / 01084第 371 1995年6月14日第 102（e）1995年6月14日PCT PCT 1994年9月15日PCT公布。 WO95 / 08000 PCT出版物 1995年3月23日的方法检测目标核苷酸序列的方法包括用示踪剂标记的至少一个核苷酸探针。 所述方法包括使用基本上包含具有侧向取代基的线性主链共聚物的试剂，其链由第一类型的重复单元和至少一种其它类型的重复单元组成，其中第一类型的单元的至少一部分 具有包含核苷酸单元的侧向取代基，这样的侧向取代基不存在于其它类型的单元上。 所述全部相同的所述核苷酸单元包含至少一个能够与所述感兴趣序列杂交的核苷酸序列和能够与探针杂交的核苷酸序列，所述试剂平均包含两个以上的所述核苷酸单元， 摩尔当量，每摩尔聚合物。 这样的试剂能够获得信号放大，从而降低灵敏度阈值。 特别是应用于生产用于检测病原体的检测或遗传疾病的诊断。