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    Magnetic particles, method for obtaining same and uses for separating molecules
    6.
    发明授权
    Magnetic particles, method for obtaining same and uses for separating molecules 有权
    磁性粒子，其获得方法和用于分离分子的方法

    公开(公告)号：US06521341B1

    公开(公告)日：2003-02-18

    申请号：US09582352

    申请日：2000-09-21

    申请人： Abdelhamid Elaissari ,  Christian Pichot ,  Bernard Mandrand

    发明人： Abdelhamid Elaissari ,  Christian Pichot ,  Bernard Mandrand

    IPC分类号： G11B516

    CPC分类号： H01F1/111 ,  B03C1/01 ,  G01N33/5434 ,  G01N2446/20 ,  G01N2446/86 ,  Y10S428/90 ,  Y10T428/2991 ,  Y10T428/2998

    摘要： The invention concerns magnetic and heat-sensitive particles having a predetermined size between 0.05 and 10 &mgr;m, each particle comprising: an inner composite core consisting of a solid organic or inorganic particle, containing within it a magnetic filler; an outer coati based on a polymer capable of interacting with at least a biological molecule, the outer polymer is heat-sensitive and has a predetermined low critical solubility temperature (LCST) ranging between 10 and 100° C. and preferably between 20 and 60° C. The invention also concerns methods for obtaining said particles and their uses. The particles are characterized in that there is an intermediate layer between the inner core and the outer layer isolating said inner core magnetic filler with respect to said functionalized outer layer. Said invention is particularly useful for separating proteins and/or nucleic acids.

    摘要翻译： 本发明涉及具有0.05-10μm之间的预定尺寸的磁性和热敏颗粒，每个颗粒包括：由固体有机或无机颗粒组成的内部复合芯，其中含有磁性填料; 基于能够与至少一种生物分子相互作用的聚合物的外涂层，外部聚合物是热敏感的，并且具有在10和100℃之间，优选在20和60℃之间的预定的低临界溶解温度（LCST） 本发明还涉及获得所述颗粒及其用途的方法。 颗粒的特征在于，在内芯和外层之间存在相对于所述官能化外层隔离所述内芯磁性填料的中间层。 所述发明对于分离蛋白质和/或核酸特别有用。

    Method for transporting or storing a protein, a virus, a prokaryotic cell, or an eukaryotic cell
    9.
    发明授权
    Method for transporting or storing a protein, a virus, a prokaryotic cell, or an eukaryotic cell 有权
    运送或储存蛋白质，病毒，原核细胞或真核细胞的方法

    公开(公告)号：US08093026B2

    公开(公告)日：2012-01-10

    申请号：US12656821

    申请日：2010-02-17

    申请人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    发明人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    IPC分类号： C12N13/00

    CPC分类号： C12N15/1013 ,  C07K1/22 ,  G01N33/5434

    摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

    摘要翻译： 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。

    Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses
    10.
    发明申请
    Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses 有权
    用于分离蛋白质或蛋白质和核酸关联的方法，或颗粒和蛋白质复合物，试剂和用途

    公开(公告)号：US20060105326A1

    公开(公告)日：2006-05-18

    申请号：US11320574

    申请日：2005-12-30

    申请人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    发明人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    IPC分类号： C12Q1/70 ,  C12Q1/68 ,  C12N1/08

    CPC分类号： C12N15/1013 ,  C07K1/22 ,  G01N33/5434

    摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

    摘要翻译： 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。