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139 条记录 (耗时 0.032 秒)

    Method and Device for Gravity Flow Chromatography
    61.
    发明申请
    Method and Device for Gravity Flow Chromatography 审中-公开
    重力流动色谱法和装置

    公开(公告)号：US20100140173A1

    公开(公告)日：2010-06-10

    申请号：US12709487

    申请日：2010-02-21

    申请人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

    发明人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

    IPC分类号： B01D15/20 ,  B01D15/22

    CPC分类号： C07K1/16 ,  B01J20/286 ,  B01J20/287 ,  B01J20/3244 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/64 ,  C12N15/1006 ,  G01N1/405 ,  G01N30/02 ,  G01N30/466 ,  G01N30/603 ,  G01N30/6043 ,  G01N30/6065 ,  G01N30/6091 ,  G01N2030/062 ,  G01N2035/103 ,  G01N2035/1053 ,  B01D15/3804 ,  B01D15/34

    摘要： The invention provides gravity chromatographic columns for the automated purification of a material (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of media positioned above a bottom frit or between a bottom and top frit. In some embodiments, the columns employ modified pipette tips as column bodies. In some embodiments, the columns employ modified plates or racks as column bodies. In some embodiments, the invention provides methods and devices for gel filtration, desalting, buffer exchange, ion exchange, ion-pairing, normal phase and reverse phase chromatography. In some embodiments, the invention provides multiplexing gravity flow chromatography on a liquid handling robotic system.

    摘要翻译： 本发明提供用于从样品溶液自动纯化材料（例如生物大分子，如肽，蛋白质或核酸）的重力色谱柱，以及制备和使用这些色谱柱的方法。 柱通常包括位于底部玻璃料之上或底部和顶部玻璃料之间的介质床。 在一些实施方案中，柱使用改进的移液管尖作为柱体。 在一些实施例中，列使用改性板或架作为柱体。 在一些实施方案中，本发明提供了用于凝胶过滤，脱盐，缓冲液交换，离子交换，离子配对，正相和反相色谱法的方法和装置。 在一些实施方案中，本发明提供了液体处理机器人系统上的多重重力流色谱法。

    Highly Parallel Gel-Free Cloning Method
    62.
    发明申请
    Highly Parallel Gel-Free Cloning Method 审中-公开
    高度平行的无凝胶克隆方法

    公开(公告)号：US20100129873A1

    公开(公告)日：2010-05-27

    申请号：US12625535

    申请日：2009-11-24

    申请人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde ,  David P. Hornby

    发明人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde ,  David P. Hornby

    IPC分类号： C12P19/34

    CPC分类号： C12N15/64 ,  C12N15/1093 ,  C12N15/66

    摘要： A highly parallel method for gene cloning is presented. PCR products can be isolated using a solid phase and ligated into a positive selection vector. The cloning method has a very high success rate and can be performed entirely by a liquid handling robot with very little human intervention.

    摘要翻译： 提出了一种高度并行的基因克隆方法。 可以使用固相分离PCR产物并连接到阳性选择载体中。 克隆方法具有非常高的成功率，并且可以完全由液体处理机器人完成，人为干预很少。

    Process for performing polynucleotide separations
    63.
    发明授权
    Process for performing polynucleotide separations 失效

    公开(公告)号：US6156206A

    公开(公告)日：2000-12-05

    申请号：US324350

    申请日：1999-06-02

    申请人： Douglas T. Gjerde ,  Robert M. Haefele ,  David W. Togami

    发明人： Douglas T. Gjerde ,  Robert M. Haefele ,  David W. Togami

    IPC分类号： B01D15/08 ,  B01D15/12 ,  B01D15/36 ,  B01D15/38 ,  C12N15/10 ,  G01N30/14 ,  G01N30/52

    CPC分类号： B01J45/00 ,  B01D15/12 ,  B01D15/366 ,  B01D15/38 ,  C12N15/101 ,  G01N2030/528 ,  G01N30/14

    摘要： The invention recognizes the deleterious effects of trace, and even undetectable amounts of multivalent cations on the separation of mixtures of polynucleotides, especially double stranded polynucleotides, and provides an improved method for separating such mixtures on wide pore, non-polar separation media by eliminating multivalent cations from the all aspects of the separation process. This is accomplished by using components in the separation process which are materials which do not release metal cations. In addition, the use of cation capture resins and other methods to remove residual traces of multivalent cations from eluting solvents, sample solutions, separation media, and system components is described. It is also important to remove any traces or organic contaminants from solvents solutions and system parts. Taking similar steps to remove residual traces of multivalent cations and organic impurities from the separation process, the invention may also be used in a batch process to separate mixtures of polynucleotide fragments.

    Method and apparatus for chromatographic quantitative analysis
    65.
    发明授权
    Method and apparatus for chromatographic quantitative analysis 失效
    色谱定量分析方法和仪器

    公开(公告)号：US4272246A

    公开(公告)日：1981-06-09

    申请号：US67855

    申请日：1979-08-20

    申请人： James S. Fritz ,  Douglas T. Gjerde ,  Gabriella Schmuckler

    发明人： James S. Fritz ,  Douglas T. Gjerde ,  Gabriella Schmuckler

    IPC分类号： G01N30/96 ,  G01N31/04 ,  G01N27/08

    CPC分类号： G01N30/96 ,  Y10T436/173076 ,  Y10T436/18 ,  Y10T436/193333

    摘要： An improved apparatus and method for the quantitative analysis of a solution containing a plurality of anion species by ion exchange chromatography which utilizes a single eluent and a single ion exchange bed which does not require periodic regeneration. The solution containing the anions is added to an anion exchange resin bed which is a low capacity macroreticular polystyrene-divinylbenzene resin containing quarternary ammonium functional groups, and is eluted therefrom with a dilute solution of a low electrical conductance organic acid salt. As each anion species is eluted from the bed, it is quantitatively sensed by conventional detection means such as a conductivity cell.

    摘要翻译： 一种用于通过离子交换色谱定量分析含有多种阴离子种类的溶液的改进的装置和方法，其利用单一洗脱液和不需要周期性再生的单一离子交换床。 将含有阴离子的溶液加入到阴离子交换树脂床中，所述阴离子交换树脂床是含有季铵官能团的低容量大网络聚苯乙烯 - 二乙烯基苯树脂，并用低电导有机酸盐的稀溶液洗脱。 由于每个阴离子物质从床上洗脱，所以通过传统的检测手段例如电导池定量地检测。

    METHODS AND DEVICES FOR NUCLEIC ACID PURIFICATION
    68.
    发明申请
    METHODS AND DEVICES FOR NUCLEIC ACID PURIFICATION 审中-公开
    用于核酸净化的方法和装置

    公开(公告)号：US20170029809A1

    公开(公告)日：2017-02-02

    申请号：US15290847

    申请日：2016-10-11

    申请人： Chris Suh ,  Carrie Loan Kim Huynh ,  Lee Hoang ,  Douglas T. Gjerde ,  Jonathan Michael Grambow

    发明人： Chris Suh ,  Carrie Loan Kim Huynh ,  Lee Hoang ,  Douglas T. Gjerde ,  Jonathan Michael Grambow

    IPC分类号： C12N15/10 ,  B01D37/03 ,  B01D15/10

    摘要： The invention provides pipette tip columns and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.

    摘要翻译： 本发明提供用于纯化包括质粒在内的核酸的移液管末端柱和自动化方法。 核酸可以从含有细胞碎片的未澄清，澄清或部分澄清的细胞裂解物中纯化。 柱通常包括位于移液管末端柱中的介质床，位于底部玻璃料上方并具有可选的顶部玻璃料。 质粒制备标准包括miniprep，midiprep，maxiprep，megaprep和gigaprep。

    Devices and Methods for Purification of Biological Cells
    70.
    发明申请
    Devices and Methods for Purification of Biological Cells 有权
    用于纯化生物细胞的装置和方法

    公开(公告)号：US20150240229A1

    公开(公告)日：2015-08-27

    申请号：US14563994

    申请日：2014-12-08

    申请人： Douglas T. Gjerde

    发明人： Douglas T. Gjerde

    IPC分类号： C12N11/06

    CPC分类号： C12N1/02 ,  C12M47/02 ,  C12N5/0081 ,  C12N5/061 ,  C12N5/0647 ,  C12N5/0693

    摘要： This invention relates to devices and methods for purifying biological cells. For example, viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Cells can be purified in a sealed chromatographic system. Methods of the invention can aid research, diagnosis and treatment of cancer.

    摘要翻译： 本发明涉及用于纯化生物细胞的装置和方法。 例如，可以使用柱（包括移液管尖端柱）从复杂的生物样品中纯化可行的肿瘤，茎，免疫和精子细胞。 细胞可以在密封的色谱系统中纯化。 本发明的方法可以帮助癌症的研究，诊断和治疗。