Process for producing peptides in E. coli
    63.
    发明授权
    Process for producing peptides in E. coli 失效
    在大肠杆菌中生产肽的方法

    公开(公告)号:US5670340A

    公开(公告)日:1997-09-23

    申请号:US352179

    申请日:1994-12-05

    摘要: The present invention is a process to express a target peptide in a large amount and accumulate the target peptide in host cells in the form of inclusion bodies. The process comprises: A) culturing host cells transformed with a plasmid able to express a gene coding for a fusion protein represented in the formula A--L--B, wherein B is a target peptide, A is a protective peptide comprising a 90-210 amino acid fragment E. coli .beta.-galactosidase, and L is a linker peptide positioned between the C-terminus of the protective peptide and the N-terminus of the target peptide and selected so that when the fusion protein is treated by an enzyme or chemical substance, the target peptide is separated, and wherein the protective peptide and linker peptide are selected so that the isoelectric point of the fusion protein in between 4.9 and 6.9; B) obtaining an insoluble fraction comprising inclusion bodies by homogenization of th cultured transformed cells; C) solubilizing the fusion protein in the inclusion bodies by treatment of the insoluble fraction with a solubilizing agent; and, D) cleaving the peptide bond between the C-terminus of the linker peptide and the N-terminus of the target peptide of the solubilized fusion protein to release the target peptide from the other peptides followed by purification of the target peptide.

    摘要翻译: 本发明是大量表达目标肽并以包涵体的形式在宿主细胞中聚集靶肽的方法。 该方法包括:A)培养用能够表达编码式ALB中表示的融合蛋白的基因的质粒转化的宿主细胞,其中B是靶肽,A是包含90-210个氨基酸片段E的保护肽 大肠杆菌β-半乳糖苷酶,L是位于保护肽的C-末端和靶肽的N末端之间的连接肽,并且被选择使得当融合蛋白被酶或化学物质处理时,靶 分离肽,并且其中选择保护性肽和接头肽,使得融合蛋白的等电点在4.9和6.9之间; B)通过均质培养的转化细胞获得包含包含体的不溶性级分; C)通过用增溶剂处理不溶性级分将融合蛋白溶解在包涵体中; 并且D)切割接头肽的C末端和溶解的融合蛋白的靶肽的N末端之间的肽键,从其他肽释放靶肽,然后纯化目标肽。

    Bass reproduction speaker apparatus
    64.
    发明授权
    Bass reproduction speaker apparatus 失效
    低音再现扬声器设备

    公开(公告)号:US5588065A

    公开(公告)日:1996-12-24

    申请号:US447429

    申请日:1995-05-23

    IPC分类号: H04R1/28 H04R3/00

    摘要: A bass reproduction speaker apparatus of the present invention includes: a cabinet with an opening, having a division member inside thereof; a speaker unit disposed at the division member; a passive radiator disposed in the opening; an amplifier for driving the speaker unit; a detector for detecting a vibration of a moving system of the speaker unit; and a feedback circuit for feeding back an output signal from the detector to the amplifier.

    摘要翻译: 本发明的低音再现扬声器装置包括:具有开口的机壳,在其内部具有分隔构件; 设置在分割部件上的扬声器单元; 设置在开口中的无源辐射器; 用于驱动扬声器单元的放大器; 用于检测扬声器单元的移动系统的振动的检测器; 以及用于将来自检测器的输出信号反馈到放大器的反馈电路。

    Production of oxide superconductors having large magnetic levitation
force
    65.
    发明授权
    Production of oxide superconductors having large magnetic levitation force 失效
    生产具有较大磁悬浮力的氧化物超导体

    公开(公告)号:US5474976A

    公开(公告)日:1995-12-12

    申请号:US39561

    申请日:1993-03-29

    摘要: A method for producing an REBaCuO oxide superconductor having large magnetic levitation force, where RE is a rare earth element selected from the group consisting of Y, Sm, Eu, Gd, Dy, Ho, Er, and Yb, involves the steps of using a raw material mixture as the starting feed, heating the raw material mixture for partial melting, followed by cooling and solidification, pulverizing and mixing the resulting solid, shaping the resulting mixture into a given shape, placing or embedding nucleates on or in the resulting shape, followed by heating for partial melting, and cooling the resulting partial melt to a substantial temperature at which a superconducting phase starts to form, followed by slow cooling, whereby the superconducting phase is preferentially formed and grown from a nucleation site.

    摘要翻译: 制备具有大的磁悬浮力的REBaCuO氧化物超导体的方法,其中RE是选自Y,Sm,Eu,Gd,Dy,Ho,Er和Yb的稀土元素,包括使用 原料混合物作为起始原料,加热原料混合物进行部分熔融,然后冷却和固化,粉碎和混合所得固体,将所得混合物成形为给定形状,将成核体放置或嵌入所形成的形状, 然后加热部分熔融,并将所得部分熔体冷却至开始形成超导相的实质温度,然后缓慢冷却,由此优选从成核位点形成和生长超导相。

    Process for producing oxide superconductor
    66.
    发明授权
    Process for producing oxide superconductor 失效
    氧化物超导体的制造方法

    公开(公告)号:US5459124A

    公开(公告)日:1995-10-17

    申请号:US144947

    申请日:1993-10-29

    摘要: A process for producing an oxide superconductor, comprising putting a formed body of raw material powders for forming an oxide superconductor on silver or silver oxide within a pan which does not melt at the melting point of silver, heating the pan to a temperature higher than the melting point of silver to bring the formed body to a semi-molten state with the formed body being floated on molten silver, cooling the pan and taking the formed body out of the re-solidified silver. This process enables a large bulk material having a diameter of 10 cm or more to be produced without occurrence of cracking.

    摘要翻译: 一种氧化物超导体的制造方法,其特征在于,将在所述锅内的银或氧化银上形成氧化物超导体的原料粉末的成形体放置在不在银熔点熔化的锅内,将所述锅加热至高于 银的熔点使成形体处于半熔融状态,其中成形体浮在熔融的银上,冷却盘并将成形体从重新凝固的银中取出。 该方法能够生产直径为10cm或更大的大体积材料而不发生开裂。

    Laser-assisted CVD process forming oxide superconducting films
    70.
    发明授权
    Laser-assisted CVD process forming oxide superconducting films 失效
    激光辅助CVD工艺形成氧化物超导薄膜

    公开(公告)号:US5276012A

    公开(公告)日:1994-01-04

    申请号:US833509

    申请日:1992-02-11

    IPC分类号: H01L39/24 B05D3/06 C23C16/00

    摘要: A method is disclosed of forming an oxide superconducting film comprising the steps of (1) mixing (a) the vapors of organic metal materials in such proportions as to provide a predetermined metal composition, or (b) said organic metal materials in such proportions as to provide a predetermined metal composition vaporizing and mixture, and (2) bringing the mixture into contact with a heated substrate so that an oxide superconducting film is formed on said substrate by a chemical vapor deposition process, wherein laser light is applied onto said substrate during formation of said oxide superconducting film on said substrate, whereby the crystallographic orientation of said oxide superconducting film being formed in the irradiated area of said substrate is such that the c-axis is parallel to the substrate.

    摘要翻译: 公开了一种形成氧化物超导膜的方法,包括以下步骤:(1)将(a)有机金属材料的蒸气以提供预定金属组合物的比例混合,或(b)所述有机金属材料的比例如 以提供预定的金属组合物蒸发和混合,和(2)使混合物与加热的基底接触,使得通过化学气相沉积工艺在所述基底上形成氧化物超导膜,其中在所述基底上施加激光 在所述衬底上形成所述氧化物超导膜,由此在所述衬底的照射区域中形成的所述氧化物超导膜的结晶取向使得c轴平行于衬底。