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101 条记录 (耗时 0.069 秒)

    Mutant luciferases
    61.
    发明授权
    Mutant luciferases 失效

    公开(公告)号：US06552179B1

    公开(公告)日：2003-04-22

    申请号：US08478205

    申请日：1995-06-07

    申请人： Keith V. Wood ,  Monika G. Gruber

    发明人： Keith V. Wood ,  Monika G. Gruber

    IPC分类号： C12N1553

    CPC分类号： C12N9/0069 ,  C12Y113/12007

    摘要： The invention provides active, non-naturally occurring mutants of beetle luciferases and DNAs which encode such mutants. A mutant luciferase of the invention differs from the corresponding wild-type luciferase by producing bioluminescence with a wavelength of peak intensity that differs by at least 1 nm from the wavelength of peak intensity of the bioluminescence produced by the wild-type enzyme. The mutant luciferases and DNAs of the invention are employed in various biosensing applications.

    Quenching reagents and assays for enzyme-mediated luminescence
    63.
    发明授权
    Quenching reagents and assays for enzyme-mediated luminescence 失效
    淬灭试剂和酶介导的发光测定

    公开(公告)号：US5744320A

    公开(公告)日：1998-04-28

    申请号：US472546

    申请日：1995-06-07

    申请人： Bruce A. Sherf ,  Keith V. Wood ,  Elaine T. Schenborn

    发明人： Bruce A. Sherf ,  Keith V. Wood ,  Elaine T. Schenborn

    IPC分类号： C12Q1/66 ,  G01N21/78 ,  G01N33/58 ,  C09K11/06 ,  C12Q1/00 ,  C12Q1/37

    CPC分类号： G01N33/581 ,  C12Q1/66 ,  Y10S435/963 ,  Y10S435/975

    摘要： The present invention relates to single and dual-reporter luminescence assays utilizing general and specific reagents to quench enzyme-mediated reactions. In one embodiment of the invention, a reagent is added to the assay which non-specifically quenches enzyme-mediated luminescent reactions. In another embodiment of the invention, a reagent is added to the assay which simultaneously quenches one enzyme-mediated luminescent reaction while activating another distinct enzyme-mediated luminescent reaction. An assay kit containing specific quench reagents, and the reagents themselves are also disclosed.

    摘要翻译： 本发明涉及使用一般和特异性试剂来淬灭酶介导的反应的单和双重报道发光测定。 在本发明的一个实施方案中，将试剂加入到非特异性淬灭酶介导的发光反应的测定中。 在本发明的另一个实施方案中，将试剂加入到测定中，同时淬灭一种酶介导的发光反应，同时激活另一种不同的酶介导的发光反应。 还公开了包含特定淬灭试剂的测定试剂盒和试剂本身。

    Kit for luciferase assay
    64.
    发明授权
    Kit for luciferase assay 失效
    荧光素酶测定试剂盒

    公开(公告)号：US5641641A

    公开(公告)日：1997-06-24

    申请号：US485834

    申请日：1995-06-07

    申请人： Keith V. Wood

    发明人： Keith V. Wood

    IPC分类号： G01N21/76 ,  C12N9/02 ,  C12Q1/66 ,  G01N33/53

    CPC分类号： C12N9/0069 ,  C12Q1/66 ,  C12Y113/12007 ,  Y10S435/81 ,  Y10S435/968 ,  Y10S435/975 ,  Y10S436/808

    摘要： Methods and compositions are provided for improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions. Thus, the invention provides methods, compositions and test kits for assaying samples for the presence of a beetle luciferase or, using a beetle luciferase-luciferin reaction, the presence of ATP. The invention also provides the complex of the coenzyme, Coenzyme A, a beetle luciferase and oxyluciferin in its exited state in the luciferase-luciferin reaction and luciferyl-CoA, the thioester of Coenzyme A and luciferin (D-(-)-2-(6'-hydroxy -2'-benzothiazolyl)-.DELTA..sup.2 -thiazoline-4-carboxylic acid).

    摘要翻译： 提供了方法和组合物，用于改善甲虫荧光素酶 - 荧光素反应中荧光素酶活性的光产生动力学。 因此，本发明提供了用于测定样品中存在甲虫荧光素酶的方法，组合物和测试试剂盒，或者使用甲虫荧光素酶 - 荧光素反应存在ATP。 本发明还提供辅酶A，甲壳素萤光素酶和荧光素酶荧光素酶在荧光素酶荧光素反应中的氧化荧光素与荧光素-CoA的络合物，辅酶A的硫酯和荧光素（D - （ - ） - 2- 6'-羟基-2'-苯并噻唑基） - DELTA 2-噻唑啉-4-羧酸）。

    Permuted and nonpermuted luciferase biosensors
    65.
    发明授权
    Permuted and nonpermuted luciferase biosensors 有权
    有色和非精子萤光素酶生物传感器

    公开(公告)号：US09359635B2

    公开(公告)日：2016-06-07

    申请号：US11732105

    申请日：2007-04-02

    申请人： Brock Binkowski ,  Frank Fan ,  Susan Wigdal ,  Keith V. Wood ,  Monika G. Wood

    发明人： Brock Binkowski ,  Frank Fan ,  Susan Wigdal ,  Keith V. Wood ,  Monika G. Wood

    IPC分类号： C12N9/02 ,  C07H21/04 ,  C12N15/00 ,  C12N1/20 ,  C12N1/00 ,  C12Q1/66

    CPC分类号： C12N9/0069 ,  C12N9/0004 ,  C12Q1/66 ,  C12Y113/12007 ,  G01N33/84

    摘要： A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.

    摘要翻译： 提供了一种修饰的荧光素酶蛋白，其是用于分子的传感器，包括cAMP，cGMP，钙，其螯合剂，激酶或磷酸酶。 还提供了循环置换的anthozoan萤光素酶蛋白质和十足配甲壳素荧光素酶蛋白质，任选地含有一个或多个异源氨基酸序列，其中至少一个直接或间接与感兴趣的分子相互作用。 进一步提供了一种修饰的anthozoan萤光素酶蛋白质和十足类甲壳类荧光素酶蛋白质，其含有一个或多个异源氨基酸序列的插入，其中至少一个与感兴趣的分子直接或间接相互作用。

    Permuted and nonpermuted luciferase biosensors
    69.
    发明申请
    Permuted and nonpermuted luciferase biosensors 有权
    有色和非精子萤光素酶生物传感器

    公开(公告)号：US20110039257A1

    公开(公告)日：2011-02-17

    申请号：US11732105

    申请日：2007-04-02

    申请人： Brock Binkowski ,  Frank Fan ,  Susan Wigdal ,  Keith V. Wood ,  Monika G. Wood

    发明人： Brock Binkowski ,  Frank Fan ,  Susan Wigdal ,  Keith V. Wood ,  Monika G. Wood

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12N15/63 ,  C12N5/10 ,  C12N9/02 ,  C12Q1/02 ,  C12Q1/66

    CPC分类号： C12N9/0069 ,  C12N9/0004 ,  C12Q1/66 ,  C12Y113/12007 ,  G01N33/84

    摘要： A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.

    摘要翻译： 提供了一种修饰的荧光素酶蛋白，其是用于分子的传感器，包括cAMP，cGMP，钙，其螯合剂，激酶或磷酸酶。 还提供了循环置换的anthozoan萤光素酶蛋白质和十足配甲壳素荧光素酶蛋白质，任选地含有一个或多个异源氨基酸序列，其中至少一个直接或间接与感兴趣的分子相互作用。 进一步提供了一种修饰的anthozoan萤光素酶蛋白质和十足类甲壳类荧光素酶蛋白质，其含有一个或多个异源氨基酸序列的插入，其中至少一个与感兴趣的分子直接或间接相互作用。