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    Ultrasensitive detection of a biological target by aptamer-conjugated gold nanoparticles
    62.
    发明授权
    Ultrasensitive detection of a biological target by aptamer-conjugated gold nanoparticles 有权

    公开(公告)号：US09891227B2

    公开(公告)日：2018-02-13

    申请号：US14701267

    申请日：2015-04-30

    申请人： Academia Sinica

    发明人： Konan Peck ,  Pan-Chyr Yang ,  Yi-Chung Chang

    IPC分类号： G01N33/53 ,  G01N33/58 ,  G01N33/543 ,  G01N33/569

    CPC分类号： G01N33/585 ,  G01N33/5308 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/56938

    摘要： Methods of assaying a biological target are disclosed. The method comprises: (a) providing a sample containing the biological target; (b) providing biotin-labeled first aptamers conjugated to a gold nanoparticle (GNP), and second aptamers conjugated to a magnetic bead, wherein the first and the second aptamers exhibit specific binding affinities to the target; (c) incubating the sample with the first and the second aptamers to obtain target-bound aptamers; (d) separating the target-bound aptamers from unbound aptamers; (e) eluting the first aptamers from the GNP; (f) incubating the eluted biotin-labeled first aptamers with streptavidin-magnetic beads and reporter gold nanoparticles (GNPs) to obtain a complex comprising: the bead, the first aptamers, attached to the bead; and the reporter GNPs captured by the bead through the first aptamers; (g) eluting the reporter GNPs captured; and (h) detecting the target by measuring and analyzing a light-scattering signal of the eluted reporter GNPs.

    Crystal structure of bifunctional transglycosylase PBP1b from E. coli and inhibitors thereof
    63.
    发明授权
    Crystal structure of bifunctional transglycosylase PBP1b from E. coli and inhibitors thereof 有权

    公开(公告)号：US09890111B2

    公开(公告)日：2018-02-13

    申请号：US14960025

    申请日：2015-12-04

    申请人： Academia Sinica

    发明人： Chi-Huey Wong ,  Che Alex Ma ,  Ting-Jen Rachel Cheng ,  Wei-Chieh Cheng

    IPC分类号： C07C235/64 ,  C12Q1/18 ,  C12Q1/48 ,  C07C235/84

    CPC分类号： C07C235/64 ,  C07C235/84 ,  C07K2299/00 ,  C12Q1/18 ,  C12Q1/48 ,  G01N2333/91102 ,  G01N2500/00

    摘要： The crystal structure at 2.16 Å resolution of the full-length bacterial bifunctional transglycosylase penicillin-binding protein 1b (PBP1b) from Escherichia coli, in complex with its inhibitor moenomycin, is provided. The atomic coordinates of the complex as well as the moenomycin binding site are provided. Three dimensional structures of amino acid residues involved in moenomycin binding and transglycosylation activity are identified. Binding site for peptidoglycan synthesis inhibitors comprising inhibitor-binding site comprises amino acid residues from at least one of transglycosylase (TG), UvrB domain 2 homolog (UB2H) and transmembrane (TM) domains of PBP1b are identified at an atomic level of resolution. Methods for rational drug design based on the atomic coordinates are provided. Methods for screening for antibiotics based on anisotropic binding assay and transglycosylase inhibitor assays are provided. Novel antibiotics based on the screening assays of the invention are disclosed.